BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0036.p (330 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbas... 547 e-157 gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbas... 381 e-107 gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydra... 126 2e-30 gi|300313479|ref|YP_003777571.1| UDP-galactose-4-epimerase [Herb... 91 2e-19 gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbas... 89 5e-19 >gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 547 bits (1410), Expect = e-157, Method: Compositional matrix adjust. Identities = 268/332 (80%), Positives = 287/332 (86%), Gaps = 18/332 (5%) Query: 1 MNILVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPESVARVQQITGRSLALVKGDIR 60 MNILVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPESV RVQQITG++L+LVKGDIR Sbjct: 1 MNILVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPESVKRVQQITGKTLSLVKGDIR 60 Query: 61 NRDQLESVLREFKCDAVIHFAGLKAVGESVEKPLMYYDNNVVGTVRLLEAMEATGVKTIV 120 NR+QLESVLREFKC+AVIHFAGLKAVGESVE+PLMYYDNNVVGTVRLLEAM+ATGVKTIV Sbjct: 61 NREQLESVLREFKCEAVIHFAGLKAVGESVEQPLMYYDNNVVGTVRLLEAMQATGVKTIV 120 Query: 121 FSSSATVYGDPQYLPLTESHPLSASNPYGRSKIVIEDMLRDYHRAHPDWKIALLRYFNPV 180 FSSSATVYGDPQYLPL E+HPLSASNPYGRSKIVIEDMLRDY+RAHPDWKIALLRYFNPV Sbjct: 121 FSSSATVYGDPQYLPLDEAHPLSASNPYGRSKIVIEDMLRDYYRAHPDWKIALLRYFNPV 180 Query: 181 GAHESGMIGEDPGGIPNNLMPYVAQVAVGRREKLSIWGGDYPTPDGTGVRDFIHVVDLAT 240 GAHESG+IGEDPGGIPNNLMP+VAQVA+GRRE+L+IWGGDYPTPDGTGVRDFIHVVDLA Sbjct: 181 GAHESGLIGEDPGGIPNNLMPFVAQVAIGRRERLNIWGGDYPTPDGTGVRDFIHVVDLAR 240 Query: 241 GHLAALQALTEPQCFAVNLGTGNGYSVLDVVKAYEKAAGKPIPYT----KPMXM------ 290 GH+AALQAL PQCFAVNLGTG GYSVLDV+KAYEKA GK +PY +P + Sbjct: 241 GHVAALQALDAPQCFAVNLGTGVGYSVLDVLKAYEKACGKTLPYAIAERRPGDIASCYAN 300 Query: 291 PIRPKRAASWAGK--------PSWDWTPCAPT 314 P KR W SW W P Sbjct: 301 PAEAKRKLGWEATLGLEAMCADSWKWQQMNPN 332 >gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 343 Score = 381 bits (979), Expect = e-107, Method: Compositional matrix adjust. Identities = 182/283 (64%), Positives = 222/283 (78%), Gaps = 1/283 (0%) Query: 3 ILVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPESVARVQQITGRSLALVKGDIRNR 62 ILVTGGAGYIGSHTCVEL+ AG+ V+V DN CNS + R+ QI+G+ V+GDIR+R Sbjct: 6 ILVTGGAGYIGSHTCVELIHAGYAVVVLDNLCNSRASVIDRIAQISGQRPHFVQGDIRDR 65 Query: 63 DQLESVLREFKCDAVIHFAGLKAVGESVEKPLMYYDNNVVGTVRLLEAMEATGVKTIVFS 122 L+ + + + +AVIHFAGLKAVGESV +PL YYDNNV G+ L EAM A GVK IVFS Sbjct: 66 AVLDGIFKSHRIEAVIHFAGLKAVGESVAQPLRYYDNNVHGSNVLFEAMAAHGVKNIVFS 125 Query: 123 SSATVYGDPQYLPLTESHPLSASNPYGRSKIVIEDMLRDYHRAHPDWKIALLRYFNPVGA 182 SSATVYGDP +P+TE PLSA+NPYGRSK+++E +L D H A P W+IALLRYFNPVGA Sbjct: 126 SSATVYGDPASVPITEEFPLSATNPYGRSKLMVEQILGDLHVADPSWRIALLRYFNPVGA 185 Query: 183 HESGMIGEDPGGIPNNLMPYVAQVAVGRREKLSIWGGDYPTPDGTGVRDFIHVVDLATGH 242 HESG+IGEDP GIPNNL+P++AQVA GRR LS++G DYPTPDGTGVRD+IHVVDLA GH Sbjct: 186 HESGLIGEDPSGIPNNLLPFIAQVADGRRAALSVYGSDYPTPDGTGVRDYIHVVDLALGH 245 Query: 243 LAALQALTE-PQCFAVNLGTGNGYSVLDVVKAYEKAAGKPIPY 284 L L+ L + P + NLGTG G SVL+++ A+E+A G +PY Sbjct: 246 LKTLRKLEQGPGVYTYNLGTGRGNSVLEMLAAFEQACGNKLPY 288 >gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydratase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 126 bits (317), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 107/311 (34%), Positives = 155/311 (49%), Gaps = 32/311 (10%) Query: 3 ILVTGGAGYIGSHTCVELLKAG-HEVIVFDNFCNSSPESVARVQQITGRSLALVKGDIRN 61 I+V GGAG IGSHT L+K E++++DNF S E++ + + V GDI Sbjct: 8 IVVIGGAGLIGSHTVDHLVKEDVKEILIYDNFLRGSRENLQNALKDPRVKIYDVGGDIMQ 67 Query: 62 RDQLESVLREFKCDAVIHFAGLKAVGESVEKPLMYYDNNVVGTVRLLEAMEATGVKTIVF 121 D L+S L D V H A L + + E P +D NV GT ++EA A GVK +V+ Sbjct: 68 TDILQSALE--GADGVFHLAALWLL-QCHEYPRSAFDVNVRGTFNVMEACVAKGVKRLVY 124 Query: 122 SSSATVYGDPQYLPLTESHPLSASNPYGRSKIVIEDMLRDYHRAHPDWKIALLRYFNPVG 181 SSSA+VYGD P+ E HP + N YG +KI E MLR +H + + LRY N G Sbjct: 125 SSSASVYGDAVREPMDEDHPYNNKNFYGATKIAGETMLRSFHHRYGLNYVG-LRYMNVYG 183 Query: 182 AHESGMIGEDPGGIPNNLMPYVAQVAVGRREKLSIWGGDYPT--PDGTGVRDFIHVVDLA 239 +D G A +AV + +I G+ PT DG+ DF+ V D Sbjct: 184 PR------QDYHG---------AYIAVIMKMLDAIDKGESPTIMGDGSEAFDFVAVEDCG 228 Query: 240 TGHLAALQALTEPQCFAVNLGTGNGYSVLDVVKAYEKAAG--KPIPYT-KPMXMPIR--- 293 ++ A++A T Q + N+GTG S+ ++ + + G KPI + + +R Sbjct: 229 LANVCAMKAETADQYY--NVGTGKRTSLKELAELLLEITGCDKPITFAPRSQATLVRNRI 286 Query: 294 --PKRAASWAG 302 PK+A+S G Sbjct: 287 GCPKKASSEIG 297 >gi|300313479|ref|YP_003777571.1| UDP-galactose-4-epimerase [Herbaspirillum seropedicae SmR1] Length = 357 Score = 90.5 bits (223), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 82/304 (26%), Positives = 131/304 (43%), Gaps = 24/304 (7%) Query: 4 LVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPESVARVQQITG----RSLALVKGDI 59 LVTG AG+IGS+ LLK V+ DNF ++ V+ + + V+GDI Sbjct: 20 LVTGVAGFIGSNLLETLLKLNQRVVGLDNFSTGFQHNLDEVKALVTPEQWANFRFVEGDI 79 Query: 60 RNRDQLESVLREFK-----CDAVIHFAGLKAVGESVEKPLMYYDNNVVGTVRLLEAMEAT 114 R D + + + V+H A L +V S+E P+ NN+ G + +L A Sbjct: 80 RKLDDCRAAMHHEALGSNIVEYVLHQAALGSVPRSIEDPITTNQNNIDGFLNMLVAARDA 139 Query: 115 GVKTIVFSSSATVYGDPQYLPLTESHPLSASNPYGRSKIVIEDMLRDYHRAHPDWKIALL 174 VK V+++S++ YGD LP E +PY +K V E + R + I L Sbjct: 140 RVKRFVYAASSSTYGDHPGLPKVEDRIGKPLSPYAVTKFVNELYADVFARTYGLQTIG-L 198 Query: 175 RYFNPVGAHESGMIGEDPGGIPNNLMPYVAQVAVGRREKLSIWGGDYPTPDGTGVRDFIH 234 RYFN G ++P G ++P A+ + E++ I G DG RDF + Sbjct: 199 RYFNIFGRR------QNPDGAYAAVIPKWIAAAI-KGEQIYING------DGETSRDFCY 245 Query: 235 VVDLATGHLAALQALTEPQCFAV-NLGTGNGYSVLDVVKAYEKAAGKPIPYTKPMXMPIR 293 V + +L A L + V N+ G+ ++ ++ + P+ + R Sbjct: 246 VDNAVQANLLAACTLQDEAANQVYNVAVGDRTTLKELYQFLRDNLSATFPHLRDAQPIFR 305 Query: 294 PKRA 297 RA Sbjct: 306 EFRA 309 >gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 347 Score = 89.0 bits (219), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 85/291 (29%), Positives = 129/291 (44%), Gaps = 28/291 (9%) Query: 4 LVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPESVARVQQITGRS----LALVKGDI 59 L+TG AG+IGS+ LL G +V DNF ++ V+ G + ++GDI Sbjct: 19 LITGVAGFIGSNLLARLLTLGQQVTGLDNFSTGRQANLEEVRAEVGAAAWANFRFIEGDI 78 Query: 60 RNRDQLESVLREFK-CDAVIHFAGLKAVGESVEKPLMYYDNNVVGTVRLLEAMEATGVKT 118 + + LR D V+H A L +V S+E P+ NNV G + +L A GV Sbjct: 79 CD---AATCLRACDGADYVLHQAALGSVSRSIEDPVRTNLNNVNGFLNMLVAARDAGVAR 135 Query: 119 IVFSSSATVYGDPQYLPLTES---HPLSASNPYGRSKIVIEDMLRDYHRAHPDWKIALLR 175 V+++S++ YGD LP E PLS PY +K V E + R + I LR Sbjct: 136 FVYAASSSTYGDHPGLPKVEEVIGKPLS---PYAVTKYVNELYAEVFARCYGMESIG-LR 191 Query: 176 YFNPVGAHESGMIGEDPGGIPNNLMPYVAQVAVGRREKLSIWGGDYPTPDGTGVRDFIHV 235 YFN G +DP G ++P A+ R +L I G DG RDF ++ Sbjct: 192 YFNVFGRR------QDPHGAYAAVIPQWV-TAMIRNGELRING------DGQTSRDFCYI 238 Query: 236 VDLATGHLAALQALTEPQCFAVNLGTGNGYSVLDVVKAYEKAAGKPIPYTK 286 ++ +L A A E N+ + S+ D+ + + P+ + Sbjct: 239 DNVVQANLLAATAGEEAVNQVYNVALNDRTSLNDLYAMMRELLVQRFPHLQ 289 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,140,495 Number of extensions: 51408 Number of successful extensions: 190 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 187 Number of HSP's successfully gapped: 8 Length of query: 330 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 244 Effective length of database: 1,212,660 Effective search space: 295889040 Effective search space used: 295889040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)