BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0033.p (261 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313655|ref|YP_003777747.1| signal peptide protein [Herbasp... 392 e-110 gi|300313766|ref|YP_003777858.1| alpha/beta hydrolase superfamil... 198 3e-52 gi|300311758|ref|YP_003775850.1| alpha/beta hydrolase superfamil... 71 7e-14 >gi|300313655|ref|YP_003777747.1| signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 392 bits (1006), Expect = e-110, Method: Compositional matrix adjust. Identities = 227/261 (86%), Positives = 246/261 (94%) Query: 1 MKSAFPRFSFKTTLGMLALAAAFTGSSAIAADASDKPSVVIVHGAFADGSDWAKVIPLLQ 60 M+++ F+ KT LG LA+AAAF+ + +A +++ SVVIVHGAFADGSDWAKVIPLLQ Sbjct: 1 MQASSRLFNLKTALGALAVAAAFSAAGPASAAPAEQASVVIVHGAFADGSDWAKVIPLLQ 60 Query: 61 AKGIKVTAVQNPLTSLADDVAAAKRAIDNQPGKVVLVGHSWGGTVITEAGNHEKVASLVY 120 AKG+KVTAVQNPLTSLADDVAAA+RAIDNQPGKVVLVGHSWGGTVITEAGNH+KVASLVY Sbjct: 61 AKGVKVTAVQNPLTSLADDVAAARRAIDNQPGKVVLVGHSWGGTVITEAGNHDKVASLVY 120 Query: 121 VAAFAPDAGQATGDLGKDLPVPPGIAKLSVDKAGFASLPPAALAADFAQDVPARQAAVMA 180 VAAFAPDAGQATGDLGKDLPVPPGIAKLSVDKAGFASLPPAALAADFAQDVPA+QAAVMA Sbjct: 121 VAAFAPDAGQATGDLGKDLPVPPGIAKLSVDKAGFASLPPAALAADFAQDVPAKQAAVMA 180 Query: 181 ATQGPIAMKAFGDKVETAAWKTRPSWYIVSTKDRMIQPALERSFAKQINAKKTELAASHV 240 ATQGPIAMKAFGDKVETAAWKTRPSWYIVSTKDRMIQPALER+FAKQINAK TEL ASHV Sbjct: 181 ATQGPIAMKAFGDKVETAAWKTRPSWYIVSTKDRMIQPALERAFAKQINAKTTELPASHV 240 Query: 241 PQQSRPAEVAKVILEAVAATR 261 PQQSRPA+VAKVILEAVAA++ Sbjct: 241 PQQSRPADVAKVILEAVAASK 261 >gi|300313766|ref|YP_003777858.1| alpha/beta hydrolase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 251 Score = 198 bits (504), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 109/239 (45%), Positives = 148/239 (61%), Gaps = 3/239 (1%) Query: 16 MLALAAAFTGSSAIAADASDKPSVVIVHGAFADGSDWAKVIPLLQAKGIKVTAVQNPLTS 75 ML L + + D + +V+VHGAFADGS W++VI LLQ KG +VTAVQNPL+S Sbjct: 1 MLFLIPLSCAAQNMMNDKHNAIDIVLVHGAFADGSSWSQVIALLQQKGYRVTAVQNPLSS 60 Query: 76 LADDVAAAKRAIDNQPGKVVLVGHSWGGTVITEAGNHEKVASLVYVAAFAPDAGQATGDL 135 LADDV+A +R ID QP +V+LVGHSWGG VIT AGN KV LVY++A APD+GQ+ + Sbjct: 61 LADDVSATRRVIDRQPHEVLLVGHSWGGAVITAAGNASKVRGLVYLSALAPDSGQSVAQM 120 Query: 136 GKDLPVPPGIAKLSVDKAGFASLPPAALAAD-FAQDVPARQAAVMAATQGPIAMKAFGDK 194 + L P ++ + D G L AA A A DV Q +AA Q PIA AFG+ Sbjct: 121 LQRLHAP--MSGMQPDADGLIWLDDAAAYAQVMAGDVSPEQVRHLAAVQQPIAAAAFGEP 178 Query: 195 VETAAWKTRPSWYIVSTKDRMIQPALERSFAKQINAKKTELAASHVPQQSRPAEVAKVI 253 + AAW+ +PSWY+++ D+ + A++R A + A+ +L +SH+ S P VA I Sbjct: 179 LVHAAWRDKPSWYLITEGDQALPTAVQRKLATLLGARTRQLISSHMSLVSHPQVVADFI 237 >gi|300311758|ref|YP_003775850.1| alpha/beta hydrolase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 243 Score = 71.2 bits (173), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 72/250 (28%), Positives = 108/250 (43%), Gaps = 42/250 (16%) Query: 38 SVVIVHGAFADGSDWAKVIPLLQAKGIKVTAVQNP-------------LTSLADDVAAAK 84 + +++HGA+ W V L +G +V ++ P L+ AD + A Sbjct: 3 NFLLIHGAWQGKWVWPAVSAELTMRGHEVHSIDLPGSGADTTPLDQVTLSLYADAIVKAI 62 Query: 85 RAIDNQPGKVVLVGHSWGG--TVITEAGNHEKVASLVYVAAFAP---DAGQATGDLGK-D 138 +AI +V LVGHS GG + +A ++Y+ A+ P D+ A DL Sbjct: 63 KAIGK---RVTLVGHSMGGIAVTAAAERAADSLARIIYLCAYVPVNGDSLSALSDLAPAR 119 Query: 139 LPVPPGIAKLSVDKAGFAS-LPPAALAADFAQDVPAR---------QAAVMAATQGPIAM 188 LP P + ++ A AS P+A F QD P +A MA P+ Sbjct: 120 LPSPVALGHDAL--AALASDTQPSARVETFMQDAPYAVAHWAAPQFRAQAMAPMTTPV-- 175 Query: 189 KAFGDKVETAAWKTRPSWYIVSTKDRMIQPALERSFAKQINAKKT-ELAASHVPQQSRPA 247 +V A+ P YIV T+DR I P L+R A + + ELA+ H P SRP Sbjct: 176 -----QVSEQAYGKLPKSYIVCTRDRAIDPVLQRVMAARSGCSRIKELASDHSPFLSRPT 230 Query: 248 EVAKVILEAV 257 E A+++ V Sbjct: 231 ETAEMLHRMV 240 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.128 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 768,122 Number of extensions: 30510 Number of successful extensions: 157 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 154 Number of HSP's successfully gapped: 3 Length of query: 261 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 178 Effective length of database: 1,226,865 Effective search space: 218381970 Effective search space used: 218381970 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)