BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0014.p (268 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313636|ref|YP_003777728.1| short-chain alcohol dehydrogena... 462 e-132 gi|300310391|ref|YP_003774483.1| 3-oxoacyl-[acyl-carrier-protein... 103 2e-23 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 90 2e-19 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 84 1e-17 gi|300313914|ref|YP_003778006.1| oxidoreductase [Herbaspirillum ... 84 1e-17 >gi|300313636|ref|YP_003777728.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 268 Score = 462 bits (1190), Expect = e-132, Method: Compositional matrix adjust. Identities = 233/262 (88%), Positives = 248/262 (94%) Query: 6 VVAAQERVFLTGASSGIGMALAQAYARRGAILGLVARRTAELEALRASLPHPERHRVYAL 65 +V A RVFLTGASSG+GMALA+AYA +GA+LGLVARRTAELEALRA+LPH ERHRVYAL Sbjct: 6 IVGASLRVFLTGASSGLGMALARAYASQGAVLGLVARRTAELEALRAALPHAERHRVYAL 65 Query: 66 DVTDHAALAAAADAFLAEFGGADVVIANAGISQGTLTEYQEDLPAFERILAVNVTATFAT 125 DVTDHAALAAAA +F+ EFGGADVVIANAGISQGTLTE+QEDL AFERILAVNVTATFAT Sbjct: 66 DVTDHAALAAAATSFMDEFGGADVVIANAGISQGTLTEFQEDLAAFERILAVNVTATFAT 125 Query: 126 FTPFVARMKAAPPAQRNRCRLVGIGSVAGIRGLPGAGAYSASKSAVISYCESLRVELRSS 185 FTPFVARMKA+P AQR RCRLVGIGSVAGIRGLPGAGAYSASKSAVISYCESLRVELR+S Sbjct: 126 FTPFVARMKASPEAQRRRCRLVGIGSVAGIRGLPGAGAYSASKSAVISYCESLRVELRAS 185 Query: 186 GIKVVTIAPGYIDTPMTRVNTYPMPFLMPVERFAERAVESIAEGVSYRVIPWQMGVVAKL 245 GIKVVTIAPGYIDTPMTRVNTY MPFLMPVERFA RAV++I EGVSYRVIPWQMGVVAKL Sbjct: 186 GIKVVTIAPGYIDTPMTRVNTYRMPFLMPVERFAARAVQTIDEGVSYRVIPWQMGVVAKL 245 Query: 246 LRLLPNWLYDMAFARAPHKKRN 267 LR+LPNWLYDMAFARAPHK+R Sbjct: 246 LRMLPNWLYDMAFARAPHKQRK 267 >gi|300310391|ref|YP_003774483.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 283 Score = 103 bits (256), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 86/261 (32%), Positives = 121/261 (46%), Gaps = 11/261 (4%) Query: 1 MANPLVVAAQERVFLTGASSGIGMALAQAYARRGAILGLVARRTAELEALRASLPHPERH 60 M P+ R++L GASSGIG LA+ GA + L ARR LE + + HPE Sbjct: 29 MNEPMADLRGRRIWLIGASSGIGAELARQALAAGARVALSARRAEVLEEVAGA--HPEAF 86 Query: 61 RVYALDVTDHAALAAAADAFLAEFGGADVVIANAGISQGTLT-EYQEDLPAFERILAVNV 119 + LDV H A + FGG D++I A + + E QED E + N+ Sbjct: 87 -IAPLDVLCHDAWRDQHARIVEAFGGVDLLIFCAARYRPERSWEVQED--EAEHTIRTNL 143 Query: 120 TATFATFTPFVARMKAAPPAQRNRCRLVGIGSVAGIRGLPGAGAYSASKSAVISYCESLR 179 + ++ + M A R + + SVAG GLPGA Y K+A+I+ E L Sbjct: 144 ASVYSALGAALPDMLA-----RGSGGIALVASVAGYVGLPGATVYGPGKAALINLAEILY 198 Query: 180 VELRSSGIKVVTIAPGYIDTPMTRVNTYPMPFLMPVERFAERAVESIAEGVSYRVIPWQM 239 +LR G+ V + PG++ T +T N + MP L ++ A I EG P + Sbjct: 199 SDLRPKGVNVYLVNPGFVKTDLTDKNDFTMPALQTPQQAASAIWRGIGEGRFEIHFPLRF 258 Query: 240 GVVAKLLRLLPNWLYDMAFAR 260 KLLR+LP L M F R Sbjct: 259 TAWLKLLRILPFRLRFMLFQR 279 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 89.7 bits (221), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 69/211 (32%), Positives = 109/211 (51%), Gaps = 15/211 (7%) Query: 13 VFLTGASSGIGMALAQAYARRGAILGLVARRTAELEALRASLPHPERHRVYALDVTDHAA 72 V +TGA+SGIG A A+ ++ GA + L R A L + SLP PER DV+ H Sbjct: 17 VIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLP-PERTAARETDVSHHEQ 75 Query: 73 LAAAADAFLAEFGGADVVIANAGI-SQGTLTEYQ-EDLPAFERILAVNVTATFATFTPFV 130 + D + FG DV++++AG+ ++G +TE ED + R+ A NV F + Sbjct: 76 VRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPED---WHRVQATNVNGVF-----YG 127 Query: 131 ARMKAAPPAQRNRCRLVGIGSVAGIRGLPGAGAYSASKSAVISYCESLRVELRSSGIKVV 190 AR +A P ++ R +V + SV+G+ AY+ASK AV + ++ ++ G+++ Sbjct: 128 AR-EALPHLEKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRIN 186 Query: 191 TIAPGYIDTPMTRVNTYPMPFLMPVERFAER 221 + P T MT P L ++FAER Sbjct: 187 AVCPSLTHTAMTADMADDPPLL---DKFAER 214 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 84.0 bits (206), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 79/236 (33%), Positives = 116/236 (49%), Gaps = 28/236 (11%) Query: 14 FLTGASSGIGMALAQAYARRGA-ILGLVARRTAELEALRASLPH------PERHRVYALD 66 +TGAS GIG A+A AR+GA ++G TA E+ A++ PE R L+ Sbjct: 12 LVTGASRGIGRAIATELARQGAKVIG-----TATSESGAAAITEYLAALGPEAGRGAVLN 66 Query: 67 VTDHAALAAAADAFLAEFGGADVVIANAGISQGTLTEYQEDLPAFERILAVNVTATFATF 126 V D A A + +FG +++ NAGI+Q L +D ++ ++A N+TA Sbjct: 67 VNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKD-EEWDSVIATNLTA-VGRL 124 Query: 127 TPFVAR--MKAAPPAQRNRCRLVGIGSVAGIRGLPGAGAYSASKSAVISYCESLRVELRS 184 + V R MKA R++ I SV G G PG Y+A+K+ V +L E+ S Sbjct: 125 SRAVLRGMMKARTG------RIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGS 178 Query: 185 SGIKVVTIAPGYIDTPMTR-VNTYPMPFLM---PVERFAERAVESIAEGVSYRVIP 236 I V +APG+IDT MTR +N ++ P+ R A E IA V++ P Sbjct: 179 RNITVNCVAPGFIDTDMTRALNEQQSAAILQQIPLGRLG--AAEEIAYAVAFLASP 232 >gi|300313914|ref|YP_003778006.1| oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 83.6 bits (205), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 65/206 (31%), Positives = 101/206 (49%), Gaps = 10/206 (4%) Query: 13 VFLTGASSGIGMALAQAYARRGAILGLVARRTAELEALRASLPHPERHRVYALDVTDHAA 72 +F+TG +SGIG LA+A+ + G + + RR A L+ + A+ P + A DV D A+ Sbjct: 8 IFITGGASGIGRGLAEAFHKLGNTVIIGGRRQALLDEVVAANPG---MQAIAFDVADPAS 64 Query: 73 LAAAADAFLAEFGGADVVIANAGISQGTLTEYQEDLPAFERILAVNVTATFATFTPFVAR 132 + AAA A + G +V++ NAGI D RIL N+ + + Sbjct: 65 IDAAAATLKARYPGLNVLVNNAGIMPFDDASGHIDDEVARRILDTNLLGPIRLTSALIDL 124 Query: 133 MKAAPPAQRNRCRLVGIGSVAGIRGLPGAGAYSASKSAVISYCESLRVELRSSGIKVVTI 192 +K P R ++ SV L YSASK+A+ SY S R LR + ++V I Sbjct: 125 LKQQP-----RATILHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRETSVRVQEI 179 Query: 193 APGYIDTPMTRVNTYPMPFLMPVERF 218 AP ++DT + + + P MP++ F Sbjct: 180 APPWVDTDLVKKSGDPRA--MPLDAF 203 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 680,792 Number of extensions: 22461 Number of successful extensions: 214 Number of sequences better than 1.0e-08: 42 Number of HSP's gapped: 170 Number of HSP's successfully gapped: 42 Length of query: 268 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 184 Effective length of database: 1,222,130 Effective search space: 224871920 Effective search space used: 224871920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)