BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0225.p (562 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312549|ref|YP_003776641.1| hemolysin activation/secretion ... 739 0.0 gi|300310625|ref|YP_003774717.1| hemolysin activation translocat... 366 e-102 >gi|300312549|ref|YP_003776641.1| hemolysin activation/secretion protein [Herbaspirillum seropedicae SmR1] Length = 584 Score = 739 bits (1909), Expect = 0.0, Method: Compositional matrix adjust. Identities = 368/563 (65%), Positives = 453/563 (80%), Gaps = 1/563 (0%) Query: 1 LSFAFSLGQWGVAHELHAQTDPATQQYQREQQRERALRRQQETTPDVRLPAESAAPALPL 60 L L Q GV AQ D +TQQ+QR+Q+RER LRRQQE PDVRLPA+ A A + Sbjct: 22 LPIVLCLCQAGVMSSAWAQADLSTQQFQRQQERERELRRQQERGPDVRLPAQPGAAAEAV 81 Query: 61 PAQETPCYPIRRVELRGEEAARFDWLLG-DEASVDGRCLGVQGINALMTRLQNAIVARGY 119 PAQE+PC+ + RV L G +ARFDWLL EA+ GRCLGVQGINA+M+ LQNAIVARGY Sbjct: 82 PAQESPCFAVHRVVLDGPGSARFDWLLDLPEANPGGRCLGVQGINAVMSALQNAIVARGY 141 Query: 120 ITTRVLAEPQDLNSGVLMLTLLPGRVRQIRFAADADPRGTQWNALPVRPGELLNLRDIEQ 179 +TTR+LA+PQD+ SG L LT++PG++R IRFA +ADPR TQWNALP PG+LLNLRDIEQ Sbjct: 142 VTTRILAQPQDIASGELRLTIVPGKIRAIRFADNADPRATQWNALPASPGDLLNLRDIEQ 201 Query: 180 GLENLKRVPTAEADIKLEPAQGEGALPGQSDLVISYRQAFPLRLAVSVDDSGSDATGKYQ 239 GLENLKRVPTAEAD+++ P++G+ A PG SDLVISY+Q P+RL +SVDDSG+ ATGKYQ Sbjct: 202 GLENLKRVPTAEADVQIAPSEGKDAAPGDSDLVISYQQGIPIRLTLSVDDSGTKATGKYQ 261 Query: 240 GSITLSYDNWWTLNDLFYVSVNHDLGGGNAGPRGSRGSTVHYSLPFGYWNLGFTTSSSDY 299 GS T+S DNW TLNDLFY+S +HDLGGG+ G RGS+ VHYSLP GYW L FT SS+ Y Sbjct: 262 GSATVSLDNWLTLNDLFYISFSHDLGGGDPGARGSQSQVVHYSLPLGYWALAFTNSSNRY 321 Query: 300 HQTIAGLTQTYLYRGQSETSEIKLARMVYRDASRKTSVSAKAFLRRASNFVNDTEIEVQR 359 HQ +AG+ QTYLY GQSET+E+KL+R+VYRDA RKT++S K F+R +SN+V+DTE++VQR Sbjct: 322 HQAVAGINQTYLYSGQSETNELKLSRLVYRDAVRKTTLSFKGFVRSSSNYVDDTEVQVQR 381 Query: 360 RRTAGWELGVGHREFIADGALDLSLAYRRGTGAFSALPAPEDVFGEGSARFSLATLDLNL 419 RR AGWE+G+ HREFI +G LDL+LAY+RGTGAF A+ APE+ FGEG++RF L T DLNL Sbjct: 382 RRVAGWEMGLAHREFIGEGTLDLALAYKRGTGAFDAMAAPEEAFGEGTSRFRLMTADLNL 441 Query: 420 SQPFSLDAPWGRQALRYTLAARGQWNGTALAPQERFSIGGRYTVRGFDGEMTLLADHGWF 479 + PF+L PWG+QA+RYTL AR QWN T L PQ++FS+GGRY+VRGFDGE LLA+ GW+ Sbjct: 442 NAPFALPMPWGKQAMRYTLGARAQWNRTPLTPQDKFSLGGRYSVRGFDGESNLLAERGWY 501 Query: 480 VRNELAAALGSSGQEFFIGLDYGEVAGPSAQLLVGKHLAGAVAGLRGGYKSLSWEAFAGT 539 VRNELAAALG+S QE + GLDYGEV GPS +LLVG+ LAGAV GLRG ++E FAG Sbjct: 502 VRNELAAALGASKQEIYAGLDYGEVGGPSTELLVGRRLAGAVLGLRGALGRTNYEVFAGV 561 Query: 540 PVSKPEGFETARLTAGFTLNWSY 562 P+S+P+ F+T+ LTAG +L WS+ Sbjct: 562 PISQPDRFKTSPLTAGMSLMWSF 584 >gi|300310625|ref|YP_003774717.1| hemolysin activation translocator protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 366 bits (940), Expect = e-102, Method: Compositional matrix adjust. Identities = 219/536 (40%), Positives = 310/536 (57%), Gaps = 28/536 (5%) Query: 45 PDVRLPAESAAPALP--LPAQETPCYPIRRVEL----RGEEAAR-----------FDWLL 87 P V LP + + L A E+ C+P++RV L + EA R F ++ Sbjct: 56 PSVSLPRQRSDEDLQSYTLAPESLCFPVQRVTLAFPEQVSEAVRRTGGFALQMDPFHFIQ 115 Query: 88 GDEASVDGRCLGVQGINALMTRLQNAIVARGYITTRVLAEPQDLNSGVLMLTLLPGRVRQ 147 A G CLG QGI ++ R+ I+ RGY TTRV QDL++G L +TL+PG + Sbjct: 116 TYLAGYAGSCLGQQGIETILRRVTGQILERGYSTTRVGIGQQDLSTGQLSITLVPGIIHA 175 Query: 148 IRFAADADPRGTQWNALPVRPGELLNLRDIEQGLENLKRVPTAEADIKLEPAQGEGALPG 207 IRF D + +A P + G LLNLRD+EQGLE +KRV + + D+++ P +PG Sbjct: 176 IRFK-DPAITASYRSAFPTQAGALLNLRDLEQGLEQMKRVASQDVDMQILPTD----VPG 230 Query: 208 QSDLVISYRQAFPLRLAVSVDDSGSDATGKYQGSITLSYDNWWTLNDLFYVSVNHDLGGG 267 QSD+V+ ++ P +L +S+DDSG+ +TGKYQ I L+ D+ +NDLF + D Sbjct: 231 QSDVVLEVKRIKPWKLTLSLDDSGAKSTGKYQAGINLAIDDLLGINDLFNAGFSSD-ADR 289 Query: 268 NAGPRGSRGSTVHYSLPFGYWNLGFTTSSSDYHQTIAGLTQTYLYRGQSETSEIKLARMV 327 RG+ G+ YS P GYW G + S+S YHQ IAGL QT++ G SE E+++ ++ Sbjct: 290 MGQARGTTGNNFSYSAPAGYWTFGVSGSTSRYHQRIAGLFQTFVSSGHSENLEVRVQKLF 349 Query: 328 YRDASRKTSVSAKAFLRRASNFVNDTEIEVQRRRTAGWELGVGHREFIADGALDLSLAYR 387 RDA +K S+ + R + ++++DTEIEVQRR T ELG HR + D LD +LA R Sbjct: 350 QRDAQQKNSLQLRIGKRWSRSYIDDTEIEVQRRNTTFAELGWMHRHYFGDVQLDATLANR 409 Query: 388 RGTGAFS-ALPAPEDVFGEGSARFSLATLDLNLSQPFSLDAPWGRQALRYTLAARGQWNG 446 G F+ A AP+ + + R+S+ T+D + PF L G Q+L Y RGQ + Sbjct: 410 WGVSWFNGAADAPDRLSDGPTYRYSMQTIDATVVAPFKL----GGQSLTYVGTLRGQHSR 465 Query: 447 TALAPQERFSIGGRYTVRGFDGEMTLLADHGWFVRNELAAALGSSGQEFFIGLDYGEVAG 506 + L ++F+IG RYTVRGFDGE+TL ++ G F+RN+L ALGS+G + GLD G + G Sbjct: 466 SRLYLTDQFAIGSRYTVRGFDGELTLASESGVFLRNDLEIALGSTGAALYAGLDAGRLYG 525 Query: 507 PSAQLLVGKHLAGAVAGLRGGYKSLSWEAFAGTPVSKPEGFETARLTAGFTLNWSY 562 PS L G LAGA GLRG L +E FAG + +PE F+TA AGF L + + Sbjct: 526 PSVANLQGDTLAGAAIGLRGAVSGLHYELFAGWALVRPEQFQTAMPAAGFNLIYQF 581 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,749,361 Number of extensions: 75277 Number of successful extensions: 211 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 204 Number of HSP's successfully gapped: 3 Length of query: 562 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 472 Effective length of database: 1,193,720 Effective search space: 563435840 Effective search space used: 563435840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)