BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0217.p (123 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310339|ref|YP_003774431.1| Rieske (2Fe-2S) domain-containi... 193 3e-51 gi|300311213|ref|YP_003775305.1| nitrite reductase ferredoxin su... 59 1e-10 >gi|300310339|ref|YP_003774431.1| Rieske (2Fe-2S) domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 112 Score = 193 bits (491), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 94/114 (82%), Positives = 101/114 (88%), Gaps = 2/114 (1%) Query: 1 MYLCHVTQLPEDGARGFDTAGAGQATIFLLRRGDQVRAWHDSCPHHGTPLPWRRDAYLDA 60 MYLCH+TQLPE+GARGFDT AGQATIF+LRRGDQVRAW DSCPHHGTPLPWRRDAY D Sbjct: 1 MYLCHITQLPENGARGFDTESAGQATIFVLRRGDQVRAWRDSCPHHGTPLPWRRDAYFDG 60 Query: 61 SGQHVVCAAHGALFDPLTGECTLGPCLGDSLTPVALRIDDDGGLHLATGEDDPL 114 +GQH+VCAAHGALFDPLTG CTLGPC GD+LT V LRIDDDGGLH+ G DPL Sbjct: 61 TGQHLVCAAHGALFDPLTGVCTLGPCQGDTLTAVPLRIDDDGGLHVDAG--DPL 112 >gi|300311213|ref|YP_003775305.1| nitrite reductase ferredoxin subunit [Herbaspirillum seropedicae SmR1] Length = 124 Score = 58.5 bits (140), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 32/104 (30%), Positives = 56/104 (53%), Gaps = 3/104 (2%) Query: 1 MYLCHVTQLPEDG-ARGFDTAGAG-QATIFLLRRGDQVRAWHDSCPHHGTPLPWRRDAYL 58 + +C +L E G + F G +A+ F++R G V + + C H L W + Sbjct: 7 ILICASAELVEGGLGKRFPVQVRGDEASGFVVRYGGVVYGYLNRCAHLPVELDWAEGEFF 66 Query: 59 DASGQHVVCAAHGALFDPLTGECTLGPCLGDSLTPVALRIDDDG 102 ++SG +++CA HGA++ P TG+C GPC G L + + ++ +G Sbjct: 67 ESSGLYLMCATHGAVYAPDTGKCAGGPCTGAHLRKI-MTVEREG 109 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.141 0.468 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 470,435 Number of extensions: 20793 Number of successful extensions: 56 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 56 Number of HSP's successfully gapped: 2 Length of query: 123 Length of database: 1,619,870 Length adjustment: 74 Effective length of query: 49 Effective length of database: 1,269,480 Effective search space: 62204520 Effective search space used: 62204520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 125 (52.8 bits)