BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0214.p (311 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310336|ref|YP_003774428.1| hypothetical protein Hsero_1003... 513 e-147 gi|300312311|ref|YP_003776403.1| enzyme involved in meta-pathway... 91 2e-19 gi|300313864|ref|YP_003777956.1| enzyme involved in meta-pathway... 60 2e-10 >gi|300310336|ref|YP_003774428.1| hypothetical protein Hsero_1003 [Herbaspirillum seropedicae SmR1] Length = 305 Score = 513 bits (1322), Expect = e-147, Method: Compositional matrix adjust. Identities = 250/311 (80%), Positives = 273/311 (87%), Gaps = 6/311 (1%) Query: 1 VKRGTVPARRLGNRAVLAVVAMIGSASMQSAQAFEGGVSPFPTGSTSEYIAALPPIPGLF 60 +KRG VPA RL A A++ A+ +A AFEGGVSPF TGSTSEYIAALPPIPGLF Sbjct: 1 MKRGFVPACRL------ATCALLTMAAGPAAYAFEGGVSPFLTGSTSEYIAALPPIPGLF 54 Query: 61 AVEQFNYSSADGLYDNKGNKRPIPFKTDVFSATTRLLASYGVEWMGAKVYSQLVLPVVSL 120 AVEQFNYSS++GLYDN GNK PIPFK+ +SATTRLLASYGV+W+GAKVYSQLVLPVVSL Sbjct: 55 AVEQFNYSSSNGLYDNNGNKLPIPFKSSAYSATTRLLASYGVQWLGAKVYSQLVLPVVSL 114 Query: 121 HSEVMGHGENHNGLSNVTVTPLLLRWDVAAHTNVTFGFDFALKTGSYDPNRTSVAVGYTS 180 H+EV GH E HNGLSNVT TP+LLRWDVAAHTNVTFGFD +L+TGSY P R SVAVGYTS Sbjct: 115 HTEVAGHSETHNGLSNVTATPVLLRWDVAAHTNVTFGFDISLETGSYSPTRPSVAVGYTS 174 Query: 181 YQPVLAIRHNDPNGLDLGISNRLLFNDKNSDTNYRSGTAYVADFIGGWNFGKWKVGVTGS 240 +QPVLA+RHN+P+GLDLGISNRLL ND+NS TNYRSGTAYVADFIGGWN GKWK GVTGS Sbjct: 175 WQPVLAVRHNNPDGLDLGISNRLLINDRNSSTNYRSGTAYVADFIGGWNVGKWKFGVTGS 234 Query: 241 YLNQFTDDRQNGTDITGNRTRSFAMGPTVAYNAGTYSINMNVQRGLYAANTAKSDAIWIN 300 YLNQFTDDRQNG DITGNR RS ++GPTVAYNAG +SINMN QRGLYAANTAKSDAIWIN Sbjct: 235 YLNQFTDDRQNGADITGNRARSLSLGPTVAYNAGPFSINMNYQRGLYAANTAKSDAIWIN 294 Query: 301 FAMPLWMGGPH 311 FAMPLWMGG H Sbjct: 295 FAMPLWMGGGH 305 >gi|300312311|ref|YP_003776403.1| enzyme involved in meta-pathway of phenol degradation protein [Herbaspirillum seropedicae SmR1] Length = 316 Score = 90.5 bits (223), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 80/295 (27%), Positives = 123/295 (41%), Gaps = 12/295 (4%) Query: 20 VAMIGSASMQSAQAFEGGVSPFPTGSTSEYIAALPPIPGLFAVE-QFNYSSADGLYDNKG 78 V +G+ AQA E G + P G T LPP V + +Y SA L D G Sbjct: 21 VVCLGAFFSLPAQAVEQGATITPIGITDFGAGMLPPSSPFGTVGVRASYYSASTLKDGAG 80 Query: 79 NKRPIPFKTDVFSATTRLLASYGVEWMGAKVYSQLVLPVVSLHSEV--------MGHGEN 130 P F DV + VE +GA+ VLP + + + + Sbjct: 81 KSIPNDFHIDVKTLALAYFYMTDVEVLGARFGVGGVLPFIDISGGLKVGTPAGPLSVSGR 140 Query: 131 HNGLSNVTVTPLLLRWDVAAHTNVTFGFDFALKTGSYDPNRT-SVAVGYTSYQPVLAIRH 189 +GL ++ V P +L W + V G TG+Y ++ + V + +Y P + + Sbjct: 141 DSGLGDMQVLPFILAWQLPPSVFVNAGLMIQAPTGAYSTSKAFNAGVNHWTYSPFVGATY 200 Query: 190 NDPNGLDLGISNRLLFNDKNSDTNYRSGTAYVADFIGGWNFGKWKVGVTGSYLNQFTDDR 249 +GL+L L N N T YRSG Y +F G + W G+ G Q +DD Sbjct: 201 ITSSGLELSTQVSLNVNTVNPATRYRSGVEYRQEFAIGQHIQSWTAGLAGYVYRQLSDDS 260 Query: 250 QNGTDITGNRTRSFAMGPTVAY-NAGTYSINMNVQRGLYAANTAKSDAIWINFAM 303 G+ GNR R +A+GP V++ G + ++ + A N A+ + + AM Sbjct: 261 GPGSG-DGNRGRVYALGPAVSFAQPGLPLVTLHGYQEFGARNRAEGYNLALRLAM 314 >gi|300313864|ref|YP_003777956.1| enzyme involved in meta-pathway of phenol degradation protein [Herbaspirillum seropedicae SmR1] Length = 316 Score = 60.5 bits (145), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 68/246 (27%), Positives = 103/246 (41%), Gaps = 36/246 (14%) Query: 51 AALPPIPGLFAVEQFNYSSADGLYDNKGNKRPIPFKTD--VFSATTRLL-ASYGVEWMGA 107 A LP PG + VE YS A L DN GN+ P D VF+ T+ + G ++ Sbjct: 39 APLPGGPGFYFVEYLTYSKATKLVDNNGNRLGFPATQDISVFAPVTQFIYVPPGAKFGNK 98 Query: 108 KVYSQLVLPVVSLHSEVMGHGENHNGLSNVTVTPLLLRWDVAAHTNVT------------ 155 ++ Q+++P V+ S + G N+ L T D++A +V Sbjct: 99 QLGFQVLVPWVA--SAKVDDGANNAVLRGNTGIG-----DISAGVSVQYDPIMGAQGPLY 151 Query: 156 ---FGFDFALKTGSYDPNRTSVAVGYT-SYQPVLAIRHNDPNGLDLGISNRLLFNDKNSD 211 F F L TG+YD N T T ++ P A L L+N +N+ Sbjct: 152 AQRFELQFILPTGAYDRNATVNPGSNTWAFDPYWAGTLWASQNLSFSWRLHYLWNARNNA 211 Query: 212 ---------TNYRSGTAYVADFIGGWNFG-KWKVGVTGSYLNQFTDDRQNGTDITGNRTR 261 ++ ++G A A+F + W VG G +L Q TD + NG D++G R R Sbjct: 212 PSAAFGNNVSSTQAGQAAHANFAAEYVLNPTWTVGAAGYWLQQVTDTKVNGADVSGRRER 271 Query: 262 SFAMGP 267 +GP Sbjct: 272 VVGLGP 277 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,064,502 Number of extensions: 46526 Number of successful extensions: 122 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 115 Number of HSP's successfully gapped: 3 Length of query: 311 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 226 Effective length of database: 1,217,395 Effective search space: 275131270 Effective search space used: 275131270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)