BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0213.p (296 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310335|ref|YP_003774427.1| transcription regulator protein... 526 e-151 gi|300309871|ref|YP_003773963.1| transcription regulator protein... 129 2e-31 gi|300310675|ref|YP_003774767.1| LysR family transcription regul... 129 4e-31 gi|300314079|ref|YP_003778171.1| LysR family transcription regul... 128 4e-31 gi|300313281|ref|YP_003777373.1| LysR family transcription regul... 120 1e-28 >gi|300310335|ref|YP_003774427.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 309 Score = 526 bits (1356), Expect = e-151, Method: Compositional matrix adjust. Identities = 260/295 (88%), Positives = 277/295 (93%) Query: 1 MRFNKLDLNLLVALDALLTELNISRAAEKIHLSQSAMSNALARLREYFDDELLIQVGRRM 60 MRFNKLDLNLLVALDALLTE++ISRAAEKIHLSQSAMSNALARLREYFDDELLIQVGRRM Sbjct: 1 MRFNKLDLNLLVALDALLTEMSISRAAEKIHLSQSAMSNALARLREYFDDELLIQVGRRM 60 Query: 61 EATPRAEVLRDAVNDVLRRIEGSIAAKPAFVPAESHREFRISVSDFSLNVLIPRVIARAH 120 E TPRAEVL+DAV+DVLRRI+GSIAA PAFVPAES REFRISVSDF+L+VLIPRV+ARAH Sbjct: 61 EPTPRAEVLKDAVHDVLRRIDGSIAALPAFVPAESTREFRISVSDFTLSVLIPRVLARAH 120 Query: 121 AEGPQIRFALMPQVQDPTRSLDRAEVDLLVLPQEFCTPDHPAEEVFRERHVCVVWRDSPL 180 AEG IRFALMPQVQDPTRSLDRAEVDLLVLPQEFCTPDHPAEEVFRERHVCVVWRDS L Sbjct: 121 AEGKHIRFALMPQVQDPTRSLDRAEVDLLVLPQEFCTPDHPAEEVFRERHVCVVWRDSAL 180 Query: 181 AKGKLTLERYMEAGHVVMVPPGANASSVEAWMASKLGFARRVEITSFSFASCVALVQGTD 240 A+G+LTLERYM +GHVVMVPPGANASSVEAWMA KLGFARRVE+TSFSFAS +ALVQGTD Sbjct: 181 AQGELTLERYMASGHVVMVPPGANASSVEAWMARKLGFARRVEVTSFSFASALALVQGTD 240 Query: 241 RIATVHGRLARKLATQLPVVLKESPLPLAEMHQMMQWHRYRTNDPGIEWLRWVXL 295 RIATVH RLA+ LA Q PVV+KESPL L EM QMMQWHRYR+NDPGI+WLR V L Sbjct: 241 RIATVHARLAQLLAPQWPVVIKESPLSLGEMRQMMQWHRYRSNDPGIQWLRRVFL 295 >gi|300309871|ref|YP_003773963.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 332 Score = 129 bits (325), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 97/297 (32%), Positives = 144/297 (48%), Gaps = 9/297 (3%) Query: 3 FNKLDLNLLVALDALLTELNISRAAEKIHLSQSAMSNALARLREYFDDELLIQVGRRMEA 62 DLNLL+ALDAL+ E +++ AA ++ LS SAMS L RLRE D LL++ GR+M Sbjct: 19 MTGTDLNLLIALDALIAEASVAGAARRLGLSASAMSRTLTRLREATGDPLLVRAGRQMVL 78 Query: 63 TPRAEVLRDAVNDVLRRIEGSI-AAKPAFVPAESHREFRISVSDFSLNVLIPRVIARAHA 121 TP AE LR+ V +V+ + + P + R+F I +D + L ++I A Sbjct: 79 TPCAEALRERVRNVVHEARSVLRPSVPELDLSRLERDFSIRANDGFIEALGAQLIEAVTA 138 Query: 122 EGPQIRFALMPQVQDPTRSLDRAEVDLLVLPQEFCTPDHPAEEVFRERHVCVVWRDSPLA 181 E P +R +P+V+ L DL + P+ + +FR+R V V PLA Sbjct: 139 EAPLVRLRFVPKVERGPVPLREGSADLEIGVASEMGPEVRLQTLFRDRFVGAVRTGHPLA 198 Query: 182 K-GKLTLERYMEAGHVVMVPPGANASSVEAWMASKLGFARRVEITSFSFASCVALVQGTD 240 +++ ERY HV P G A + +A++ G RRV SF +A+V+ +D Sbjct: 199 ALRRVSAERYCAFDHVSASPRGVAAGPLHEALAAR-GLVRRVVAVVPSFPGALAVVRTSD 257 Query: 241 RIATVHGRL------ARKLATQLPVVLKESPLPLAEMHQMMQWHRYRTNDPGIEWLR 291 + V G RK + + + E PLPL + + WH DP WLR Sbjct: 258 LVTMVPGSFLTRAGAERKDGSAAGIHVFELPLPLEPIIVSLMWHPRVELDPAHRWLR 314 >gi|300310675|ref|YP_003774767.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 309 Score = 129 bits (323), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 90/300 (30%), Positives = 150/300 (50%), Gaps = 11/300 (3%) Query: 3 FNKLDLNLLVALDALLTELNISRAAEKIHLSQSAMSNALARLREYFDDELLIQVGRRMEA 62 FN +DLNLL A+ E +++ A ++HLSQ A+S AL RLR+ FDD L I+ M+ Sbjct: 4 FNNMDLNLLRVFQAIADERSLTLAGNRLHLSQPAVSYALGRLRQIFDDPLFIRTKEGMQP 63 Query: 63 TPRAEVLRDAVNDVLRRIEGSIAAKPAFVPAESHREFRISVSDFSLNVLIPRVIARAHAE 122 TP A L +N L+ ++ ++ F PA+S R FR S++D + + +P + A+ Sbjct: 64 TPAALELAKPINRALQAVQDALRYTERFDPAQSTRVFRASMTDVAEMMFLPDLCEILTAQ 123 Query: 123 GPQIRFALMPQVQDPTRSLDRA----EVDLLVLPQEFCTPDHPAEEVFRERHVCVV-WRD 177 P +R + Q P L+ A ++D P E +FRE +VC++ RD Sbjct: 124 APDVRLQVE---QVPPAHLEEALRTGQLDFAFGNLPALKPVTCHELLFRETYVCLMRQRD 180 Query: 178 SPLAKGKLTLERYMEAGHVVMVPPGANASSVEAWMASKLGFARRVEITSFSFASCVALVQ 237 + +L L+ ++ HV++ ++ VE KLG RR+ ++ F ++ Sbjct: 181 GLPQRAQLELDEFLAMSHVLVESAESSHHQVENSF-QKLGVQRRISLSIPHFTVLPRILG 239 Query: 238 GTDRIATVHGRLARKLATQLPVVLKESPLP--LAEMHQMMQWHRYRTNDPGIEWLRWVXL 295 +D +ATV R+AR ++ P V LP + + + WH+ +D G WLR + + Sbjct: 240 KSDLVATVPARIARLFHSEHPGVFVSYQLPVNIPVVEVTLHWHQSFDHDAGNSWLRQIVI 299 >gi|300314079|ref|YP_003778171.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 319 Score = 128 bits (322), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 90/303 (29%), Positives = 152/303 (50%), Gaps = 14/303 (4%) Query: 1 MRFNKLDLNLLVALDALLTELNISRAAEKIHLSQSAMSNALARLREYFDDELLIQVGRRM 60 M F LDLNLL DA++TE N++RAA + ++Q A+SNAL RLR+ +D+L+I+ + Sbjct: 1 MSFLTLDLNLLRVFDAVMTEQNLTRAANLLAMTQPAVSNALRRLRDTLNDDLVIRTAHGV 60 Query: 61 EATPRAEVLRDAVNDVLRRIEGSIAAKPAFVPAESHREFRISVSDFSLNVLIPRVIARAH 120 + TPRAE L V L +E IA + +F + + FR+++ D + + +P ++ Sbjct: 61 KPTPRAEELWPTVRRALSELEAVIAPE-SFDISRAQNTFRMAMVDATAALWLPSLVRTIM 119 Query: 121 AEGPQIRFALMP-QVQDPTRSLDRAEVDLLV--LP---------QEFCTPDHPAEEVFRE 168 + P+I+ +MP +DP L R ++DL V P Q ++ Sbjct: 120 RDAPRIKIRMMPLTTRDPRAMLLRGDIDLAVGFFPGVTAQLADGQGMAKSQISHARLYTG 179 Query: 169 RHVCVVWRDSPLAKGKLTLERYMEAGHVVMVPPGANASSVEAWMASKLGFARRVEITSFS 228 ++VCV+ + PLA LTL+ Y A H+++ G + ++ +A + R++ +T Sbjct: 180 QYVCVMHKSHPLAGQPLTLDSYCNADHLLVSLSGKPHAVIDDVLAG-MNRERKILLTVNQ 238 Query: 229 FASCVALVQGTDRIATVHGRLARKLATQLPVVLKESPLPLAEMHQMMQWHRYRTNDPGIE 288 F + +V ++ I + L +V+KE P E H M WH +P + Sbjct: 239 FFTAGRIVASSELITVLPRHLVAATGMAEALVVKELPFQTPEEHVDMLWHERDARNPAHK 298 Query: 289 WLR 291 WLR Sbjct: 299 WLR 301 >gi|300313281|ref|YP_003777373.1| LysR family transcription regulator [Herbaspirillum seropedicae SmR1] Length = 323 Score = 120 bits (302), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 90/300 (30%), Positives = 147/300 (49%), Gaps = 21/300 (7%) Query: 5 KLDLNLLVALDALLTELNISRAAEKIHLSQSAMSNALARLREYFDDELLIQVGRRMEATP 64 DLNLL A+ E ++S AA K+ +SQ A+S AL +LR D L ++ GR M+ TP Sbjct: 3 NFDLNLLPIALAVFEERSVSAAARKLDMSQPAVSVALNKLRSALGDPLFVKTGRGMQPTP 62 Query: 65 RAEVLRDAVNDVLRRIEGSIAAKPAFVPAESHREFRISVSDFSLNVLIPRVIARAHAEGP 124 RA L ++L+R+ + + F PA + + F +++SD +L+PR++ R E P Sbjct: 63 RAVSLIGPTREILQRVHAEVLSSDQFTPATTTKRFVLALSDIGELMLLPRLLERIRDEAP 122 Query: 125 Q---IRFALMPQVQDPTRSLDRAEVDLLVLPQEFCTPDHPAEEVFRER-----HVCVVWR 176 + + P + +L+ EVDL + PD F++R VC++ Sbjct: 123 HASVVTVTMAP--AELVAALESGEVDLAI----GYFPDLRNRNFFQQRLFSQDFVCLLRA 176 Query: 177 DSPLAKGKLTLERYMEAGHVVMVPPGANASSVEAWMASKLGFARRVEITSFSFASCVALV 236 PL K++LE Y AGH+V+ G + E ++ + RRV +++ F + LV Sbjct: 177 GHPLPAQKISLEHYQAAGHIVVKAEGRDQPMCERFLLRR-KIRRRVVLSTAHFMAIPLLV 235 Query: 237 QGTDRIATV---HGRLARKLATQLPVVLKESPLPLAEMHQMMQWHRYRTNDPGIEWLRWV 293 +D +ATV G + A + +V +P+ ++ Q WHR D G WLR + Sbjct: 236 ATSDLVATVPRAFGEFVVRFAG-IRLVEPGFEMPMLDLKQ--HWHRKYHKDAGNAWLRGL 292 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 886,413 Number of extensions: 35502 Number of successful extensions: 227 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 221 Number of HSP's successfully gapped: 8 Length of query: 296 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 211 Effective length of database: 1,217,395 Effective search space: 256870345 Effective search space used: 256870345 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)