BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0211.p (327 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane ... 192 3e-50 gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane ... 187 1e-48 gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane ... 144 9e-36 gi|300312829|ref|YP_003776921.1| Fe2+-dicitrate sensor membrane ... 130 1e-31 gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [H... 109 3e-25 >gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 326 Score = 192 bits (488), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 119/320 (37%), Positives = 166/320 (51%), Gaps = 15/320 (4%) Query: 9 NDDIVMAAIDWMVRLQSGNASDHEHEACRRWRAEQPQHELAWQRLQSLSERVATLPRALA 68 ++ I+ A W++ + G +WR P+HE AW R L E A LP Sbjct: 12 DEAILTEAATWLMEMHEGPLDAARRSKLAQWRQRSPEHERAWSRAALLQETFAALP---- 67 Query: 69 HGSIGASEARTRNNRRFALKTLCVATGAGLLAASAARWTPWQAWM-----AGYTTRTGER 123 G A + RR A+K L A L A W + A A T GER Sbjct: 68 PGGATALKQVGPAGRRKAIKLLAYA-----LTLGPAGWLAYHALSGPEQGARIATAIGER 122 Query: 124 RRITLADGTLLQLNSATAVDVSFDEQQRLIRLHRGEVLITTGHEERPSYRPFLVQTRLGQ 183 R + L DG+ L LN+ T + + F QRL+RL RGE+++ T + + RPFLV TR G Sbjct: 123 REVQLDDGSRLWLNTDTRITLDFTPAQRLLRLQRGEIMVLTAPDRQQPPRPFLVATRQGS 182 Query: 184 ARALGTRFQLRDAEEGMRLAVYEGAVEFRSQNENAPVRVDAGRQGLFDAQGLVGRLVAAN 243 R LGTRF +R+ ++ + +AV +GAVE + + +G Q F G + Sbjct: 183 LRPLGTRFTVRERDQAVEVAVLQGAVEITPASSAQQTVLPSGMQTRFTRSGSAAPRPCDD 242 Query: 244 EQAIAWTDGVIVADRMPLGQLVAELDRYLPGKLRCDPAVAGLPISGVFPLDQPARILAAI 303 QA AW+ G+++A+RMPL Q + EL RY G L+ DPA+AGL +SGVFPL R+L + Sbjct: 243 TQA-AWSRGLLIAERMPLAQFIGELGRYRSGLLQADPAIAGLEVSGVFPLADTDRVLRLL 301 Query: 304 ARTLPVRVDTFTDYWITLRP 323 A+TLPVRV T YW+ + P Sbjct: 302 AQTLPVRVQQLTRYWVRVLP 321 >gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 187 bits (475), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 119/308 (38%), Positives = 173/308 (56%), Gaps = 13/308 (4%) Query: 16 AIDWMVRLQSGNASDHEHEACRRWRAEQPQHELAWQRLQSLSERVATLPR----ALAHGS 71 A++W++ LQ+ + EA WR+ P HE AW+R++S+ R+ L ALA + Sbjct: 24 ALEWLLELQTQPLAPSTLEAWEAWRSAHPDHERAWRRIESVRGRLQALASPVNAALAQAA 83 Query: 72 IGASEARTRNNRRFALKTLCVATGAGLLAASAARWTPWQAWMAGYTTRTGERRRITLADG 131 + S NRR +KTL + G +A R PWQ W A T G++RR+ L DG Sbjct: 84 LTPS-----GNRRQVIKTLALLVFGGGVAWGLTRSEPWQQWRADLRTDIGQQRRLVLEDG 138 Query: 132 TLLQLNSATAVDVSFDEQQRLIRLHRGEVLITTGHEERPSYRPFLVQTRLGQARALGTRF 191 + L LN+ +AV+++F ++R +RL GE+L+TTG RPFLV+T G A ALGTR+ Sbjct: 139 SQLVLNTHSAVNLAFSAEERRLRLISGELLVTTGKAG--GGRPFLVETAQGLAYALGTRY 196 Query: 192 QLRDAEEGMRLAVYEGAVEFRSQNENAPVRV-DAGRQGLFDAQGLVGRLVAANEQAIAWT 250 +R ++V+EGAV + RV AG+Q F AQG VG + A+E +AWT Sbjct: 197 AVRQRAGRTEVSVWEGAVRLVPRQAAQQARVLQAGQQAAFSAQG-VGDVTTADETRMAWT 255 Query: 251 DGVIVADRMPLGQLVAELDRYLPGKLRCDPAVAGLPISGVFPLDQPARILAAIARTLPVR 310 DGVIVA M L +AEL RY +L C PAV+G+ ISG +P+ ++A +A L ++ Sbjct: 256 DGVIVARGMRLDAFLAELGRYTTDRLSCAPAVSGVRISGSYPVADSDAVIATLAAALGLQ 315 Query: 311 VDTFTDYW 318 +W Sbjct: 316 AQLRQRFW 323 >gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 144 bits (363), Expect = 9e-36, Method: Compositional matrix adjust. Identities = 116/342 (33%), Positives = 176/342 (51%), Gaps = 31/342 (9%) Query: 1 MGSERTAPNDDIVMAAIDWMVRLQS--GNAS--DHEHEACRRWRAEQPQHELAWQRLQSL 56 M S D ++ AI W+V+L++ G+A+ WRA P+HE W L + Sbjct: 1 MPSNPIPLQDPVLDQAIAWLVKLRAFPGDAAVAPELQAGIAAWRAADPRHEQVWSELMAS 60 Query: 57 SERVATL-----PRALAHGSIGASEARTRNNRRFA--LKTLCVATGAGLLAASAARWTPW 109 E+ L P + HG + R ++++ + + GA L A AAR Sbjct: 61 EEQFQRLVALPVPASQWHGPLKRLNQRRQHSQSRRRWMGNAMLGLGAIALGAVAAR---- 116 Query: 110 QAWM------AGYTTRTGERRRITLADGTLLQLNSATAVDVSFDEQQRLIRLHRGEVLIT 163 QA + A Y TRTG++R++ L DGT L LN++++V + FD+ +RL+ L +GE+ I Sbjct: 117 QAGLFEALDGASYATRTGDQRQLRLGDGTALALNTSSSVQLRFDDTRRLLLLRQGEIFID 176 Query: 164 TGHEER-PSYRPFLVQTRLGQARALGTRFQLRDAEEGMRLAVYEGAVEFRSQNENAPVRV 222 TG + + P +RPF VQT + +ALGTRF +R +G +L V EG V + P+ Sbjct: 177 TGADAQWPHHRPFWVQTAHARLQALGTRFDVRQHGDGTQLTVLEGRVAVHVAGVD-PLVA 235 Query: 223 DAGRQGLFDAQGLVGRLV-----AANEQAIAWTDGVIVADRMPLGQLVAELDRYLPGKLR 277 G + D GRLV A+ AW + +V +M L +VA+L RY G L+ Sbjct: 236 QPGE--IVDIHP-DGRLVRRAARASAMDPTAWLEQSLVVRQMRLADVVAQLARYRSGWLQ 292 Query: 278 CDPAVAGLPISGVFPLDQPARILAAIARTLPVRVDTFTDYWI 319 CDPAVA +SGVF L L A+A+ LP+ ++ T +W+ Sbjct: 293 CDPAVADWQVSGVFQLQDTDVALEALAQALPLTIERRTRFWV 334 >gi|300312829|ref|YP_003776921.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 316 Score = 130 bits (327), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 107/324 (33%), Positives = 160/324 (49%), Gaps = 18/324 (5%) Query: 9 NDDIVMAAIDWMVRLQSGNASDHEHEACRRWRAEQPQHELAWQRLQSLSERVATLPRALA 68 ++ IV AI+WM++L+ G+ E A +W+A P+H +A+ L S S + +PR Sbjct: 4 DEKIVEQAIEWMLQLEQGDLPPGERAAFEQWKAADPRHAMAYANL-SQSVQQFDVPR--- 59 Query: 69 HGSIGASEARTRN------NRRFALKTLCVATGAGLLAASAA-RWTPWQAWMAGYTTRTG 121 +GA+ RN RR ALK + G + AA+ R TP A A T TG Sbjct: 60 --QVGAAPVILRNTLQQKSKRRKALKRIAALAGLAVGAAALLDRVTPVAALTADLHTGTG 117 Query: 122 ERRRITLADGTLLQLNSATAVDVSFDEQQRLIRLHRGEVLITTGHEERPSYRPFLVQTRL 181 +R+R TLADG+++ LN+ +A+D R +RL GE+ + + PF ++T Sbjct: 118 QRQRTTLADGSIITLNARSAIDHLISPVLRQVRLLEGEIQVQIAAA---NALPFSIETVH 174 Query: 182 GQARALGTRFQLRDAEEGMRLAVYEGAVEFRSQNENAPVRVDAGRQGLFDAQGLVGRLVA 241 G A L L + A ++N + AG++ FD Q +G A Sbjct: 175 GSVSATAGTLMLGLQPLRTDLVAQQAAALLSTRNGQHAT-LAAGQRTWFD-QDEIGAPRA 232 Query: 242 ANEQAIAWTDGVIVADRMPLGQLVAELDRYLPGKLRCDPAVAGLPISGVFPLDQPARILA 301 A +W +G A L ++VA L Y G LR DPAVA L ISG FPLD R L+ Sbjct: 233 AGGAENSWVEGYYTASDNSLEEVVAALRPYRKGLLRLDPAVAQLRISGAFPLDDTDRALS 292 Query: 302 AIARTLPVRVDTFTDYWITLRPRA 325 A+A LP+ V+ + YW+++ R+ Sbjct: 293 ALASALPITVNRVSPYWVSISGRS 316 >gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 109 bits (273), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 109/335 (32%), Positives = 167/335 (49%), Gaps = 22/335 (6%) Query: 7 APNDDIVMAAIDWMVRLQSGNASDHEHEACR--RWRAEQPQHELAWQRLQSLSERVATL- 63 A ++DI A DW+VRL +G+A+ + A RW+ + QH A + LQ + L Sbjct: 10 ASSEDITAMAADWIVRLSAGDAAQQQETAAAFARWQGQSRQHAQAARELQGVLAHCEGLL 69 Query: 64 ------PRALAHGSIGASEARTRNNR-RFALKTLCVATGAGLLAASAARWTPWQAWMAGY 116 P L G A R R R A+ L +A GL + P + +A Sbjct: 70 HSAQGDPSPLLAGLEAAMAPSPRPGRGRRAVAALALACCIGLPLWLGLQAYPARYLLADM 129 Query: 117 TTRTGERRRITLADGTLLQLNSATAVDVSFDEQQRLIRLHRGEVLITTGHEERPSYRPFL 176 T TG TLADG+ L L+ ++A + FD Q+R + L +GE+L+ R RPF+ Sbjct: 130 RTATGHWDARTLADGSRLLLSGSSAANFHFDAQRRTVELVQGELLVEVA---RDPARPFV 186 Query: 177 VQTRLGQARALGTRFQLRDAEEGMRLAVYEGAVEFR--SQNENAPVRVDAGRQGLFDAQG 234 +QT G RALGTRF +R G +++ E V + + AP+ ++AG++ G Sbjct: 187 IQTADGSIRALGTRFLVRRDRAGTLVSMLESKVSIQPGADTTQAPLVLEAGQRVRLHG-G 245 Query: 235 LVGRLVAANEQAI--AWTDGVIVADRMPLGQLVAELDRYLPGKLRCDPA-VAGLPISGVF 291 G L + A+ AW +V++ PL +++ EL+R+ PG++ D A +AGL ++ V Sbjct: 246 AAGPLEHIDAAALERAWRRHQLVSNARPLTEVLDELNRHRPGRILYDQAQLAGLHMTVVL 305 Query: 292 PLDQPARILAAIARTLP-VRVDTFTDY--WITLRP 323 PLD R L + +P +RV FT Y +I L P Sbjct: 306 PLDDTQRALQLLVDGVPGLRVRQFTPYLTYIDLAP 340 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,056,639 Number of extensions: 44260 Number of successful extensions: 190 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 173 Number of HSP's successfully gapped: 6 Length of query: 327 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 242 Effective length of database: 1,217,395 Effective search space: 294609590 Effective search space used: 294609590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)