BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0209.p (381 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC t... 577 e-166 gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC t... 350 1e-97 gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC t... 251 1e-67 gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC t... 174 1e-44 gi|300313247|ref|YP_003777339.1| branched-chain amino acid ABC t... 142 4e-35 >gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 380 Score = 577 bits (1487), Expect = e-166, Method: Compositional matrix adjust. Identities = 286/379 (75%), Positives = 319/379 (84%), Gaps = 2/379 (0%) Query: 3 KIKMIPVATALVAAFSFAGAAQAQEVIKIGHVAPLTGPNAHIGKDNENGARMAVDELNAK 62 K +IP+ A+ +FAGAA +QEV+KI HV PL+GPNAH+GKDNENGARMAVDELNAK Sbjct: 4 KSAIIPLTAAI--GLAFAGAAYSQEVVKIAHVGPLSGPNAHMGKDNENGARMAVDELNAK 61 Query: 63 GFEIGGKKVTFQLVPQDDASDPKQATTVAQALVDAKVKGVVGHMNSGTTIPASKIYFDAG 122 GF IGGKKV F+LV +DDASDPKQAT VA LVD KV V+GH+NSGTTIPASKIY DAG Sbjct: 62 GFTIGGKKVKFELVGEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYSDAG 121 Query: 123 IPQISPSATNPKYTQQGFNTAFRVVANDGQLGGVLGRYAVNELKGKNVAVIDDRTAYGQG 182 IPQ+SPSATNPKYTQQGF TAFRVVAND QLG LG+YAV +L K +AVIDDRTAYGQG Sbjct: 122 IPQVSPSATNPKYTQQGFKTAFRVVANDAQLGAALGKYAVQKLGTKQIAVIDDRTAYGQG 181 Query: 183 VAEEFRKSALAAGATIVATQYTTDKATDFNAILTSVKSKKPDLVFFGGMDAVAGPMLRQM 242 VA+EF K A AAG TIV TQYT DKATDFNAILTS+K KKPD+VFFGGMDAV GPMLRQM Sbjct: 182 VADEFAKGAKAAGGTIVGTQYTNDKATDFNAILTSLKGKKPDVVFFGGMDAVGGPMLRQM 241 Query: 243 DQLGVAAKFMGGDGICTTELPSLAGAGLKDSEVVCAEAGGVTEAGKKPLEDFKAAYKKKF 302 QLG++AKFMGGDGICT LP LAG GL D +V+CAEAGGV EAGKK ++DF+AAYKKKF Sbjct: 242 KQLGISAKFMGGDGICTGSLPGLAGDGLGDDQVICAEAGGVDEAGKKGMDDFRAAYKKKF 301 Query: 303 NQDVVIYAPYTYDALMTLADAMKQAGSSDPKVYLPVLAKIKHKGVTGEIAFDAKGDILNG 362 DVV A Y YDA MT+ADAM +AGS+DPK YLP LAKI HKGVTG IAFDAKGDI +G Sbjct: 302 GIDVVYNAAYAYDATMTVADAMAKAGSADPKKYLPELAKISHKGVTGVIAFDAKGDIKDG 361 Query: 363 TLTLYTYKGGKRTLLSVVK 381 +LTLYTYKGG+RTLL+V K Sbjct: 362 SLTLYTYKGGQRTLLAVTK 380 >gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 378 Score = 350 bits (898), Expect = 1e-97, Method: Compositional matrix adjust. Identities = 188/369 (50%), Positives = 242/369 (65%), Gaps = 6/369 (1%) Query: 4 IKMIPVATALVAAFSFAGAAQAQEVIKIGHVAPLTGPNAHIGKDNENGARMAVDELNAKG 63 + +I + AL+ AF+ A Q +V+KIG +PLTGP A GKDN+ G MA++ LNA+ Sbjct: 8 LNIIAASLALIPAFAMA---QETQVVKIGFSSPLTGPQASAGKDNQGGLMMAIERLNAQP 64 Query: 64 FEIGGKKVTFQLVPQDDASDPKQATTVAQALVDAKVKGVVGHMNSGTTIPASKIYFDAGI 123 +GGKK+ F ++ +DD +DPK VAQ L D VK +VG NSG TIPAS++Y DAGI Sbjct: 65 ITVGGKKIKFDVIAEDDQADPKSGVAVAQKLADQGVKAIVGPYNSGVTIPASRVYNDAGI 124 Query: 124 PQISPSATNPKYTQQGFNTAFRVVANDGQLGGVLGRYAVNELKGKNVAVIDDRTAYGQGV 183 ++ A+NPK TQQGF T FRV A+D QLGG + YA ELK K VAVIDDRTAYGQG+ Sbjct: 125 -VVATVASNPKITQQGFATLFRVAASDSQLGGKMALYAAKELKFKRVAVIDDRTAYGQGL 183 Query: 184 AEEFRKSALAAGATIVATQYTTDKATDFNAILTSVKSKKPDLVFFGGMDAVAGPMLRQMD 243 A+EF K A A G +V+T +T DKATDF AILTS+K KKPD VF GG GP+ RQM Sbjct: 184 AQEFIKVAKANGIDVVSTDFTNDKATDFTAILTSIKGKKPDAVFLGGYAPQGGPIKRQMK 243 Query: 244 QLGVAAKFMGGDGICTTELPSLAGAGLKDSEVVCAEAGGVTEAGKKPLEDFKAAYKKKFN 303 QLGV MGGDGIC+ E+ L G + +S V C + G + + K+ + F Y+KK+N Sbjct: 244 QLGVDVPLMGGDGICSPEMGRLGGDAIGES-VYCTQGGTMLDKAKEG-KVFSDEYQKKYN 301 Query: 304 QDVVIYAPYTYDALMTLADAMKQAGSSDPKVYLPVLAKIKHKGVTGEIAFDAKGDILNGT 363 + YA YD +M +A AMKQA S DPK + P LAKI +KGV G+ FDA D+ Sbjct: 302 RPAETYAVSFYDGMMLIAQAMKQANSVDPKQFGPALAKISYKGVAGQYDFDANHDLKQSP 361 Query: 364 LTLYTYKGG 372 +T+Y +K G Sbjct: 362 VTVYRFKDG 370 >gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 394 Score = 251 bits (640), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 127/348 (36%), Positives = 206/348 (59%), Gaps = 5/348 (1%) Query: 24 QAQEVIKIGHVAPLTGPNAHIGKDNENGARMAVDELNAKGFEIGGKKVTFQLVPQDDASD 83 Q + IG PL+GP+ +GK +NG ++A+ E N +G IGGK +L+ QDD +D Sbjct: 35 QETATLAIGFAGPLSGPSGGVGKSMQNGVQLALAEANRRGLRIGGKSWQLRLIAQDDRAD 94 Query: 84 PKQATTVAQALVDAKVKGVVGHMNSGTTIPASKIYFDAGIPQISPSATNPKYTQQGFNTA 143 P A VA++LV +V VVGH SGT++ A+ +Y AG+ Q++PSA + + T + Sbjct: 95 PATAEFVARSLVQQQVIAVVGHWTSGTSLAAAPVYHRAGVIQVTPSAMSRRLTAMSYPRI 154 Query: 144 FRVVANDGQLGGVLGRYAVNELKGKNVAVIDDRTAYGQGVAEEFRKSALAAGATIVATQY 203 FR + N+ LG + YAV++L+ + + IDDRT +GQG+AE+F ++A GA +V Sbjct: 155 FRTIPNNESLGRLAAGYAVDKLETRTIVTIDDRTPFGQGLAEQFARTASERGAQVVGRYS 214 Query: 204 TTDKATDFNAILTSVKSKKPDLVFFGGMDAVAGPMLRQMDQLGVAAKFMGGDGICTTELP 263 +DK +DFNA L + +PDL+FFGG+D AG + + + +L + A+ M G T LP Sbjct: 215 VSDKTSDFNAALLEARKLQPDLIFFGGLDWQAGVLAKSIRRLKLHARLMASPG--TLGLP 272 Query: 264 SLAGAGLKDSEVVCAEAGGVTEAGKKP-LEDFKAAYKKKFNQDVVIYAPYTYDALMTLAD 322 L AG + + E G + K P + F+ Y + F+ D+ +YA + Y+A + Sbjct: 273 FLMRAGPDANGALVLEPGPPQD--KMPGWKSFRQRYSENFDSDMDLYAVFAYEAAQAIIQ 330 Query: 323 AMKQAGSSDPKVYLPVLAKIKHKGVTGEIAFDAKGDILNGTLTLYTYK 370 ++QAGS+DP+ + +++ +GV+G +AF+ +GD+L + T+Y K Sbjct: 331 GIRQAGSADPERIAEAMHRLRFEGVSGPVAFNEEGDLLQPSFTMYEVK 378 >gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 373 Score = 174 bits (441), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 117/344 (34%), Positives = 182/344 (52%), Gaps = 8/344 (2%) Query: 27 EVIKIGHVAPLTGPNAHIGKDNENGARMAVDELNAKGFEIGGKKVTFQLVPQDDASDPKQ 86 E +KI P+TG A G + GA A++++NA G G K F+ V DDA +PKQ Sbjct: 27 ETVKIAIAGPMTGAVAQYGDMVKAGALTAIEQINAAGGANGNK---FEAVMMDDACEPKQ 83 Query: 87 ATTVAQALVDAKVKGVVGHMNSGTTIPASKIYFDAGIPQISPSATNPKYTQ-QGFNTAFR 145 A VA +V +K V+GH+ SG+TIPAS IY + G+ I+PSAT P+ T+ + FR Sbjct: 84 AVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGVVMITPSATAPQLTEAKPHKFIFR 143 Query: 146 VVANDGQLGGVLGRYAVNELKGKNVAVIDDRTAYGQGVAEEFRKSALAAGATIVATQYTT 205 + D Q G RY + +LK K VAV+ D+ +YGQGVA + + AA +V + Sbjct: 144 TIGRDDQQGPAAARYVIEKLKPKKVAVLHDKQSYGQGVASSVKAALDAAKVPVVVFEGIN 203 Query: 206 DKATDFNAILTSVKSKKPDLVFFGGMDAVAGPMLRQMDQLGVAAKFMGGDGICTTELPSL 265 +D++AI+T +KS+ D V+FGG G ++RQ + G+ A FMG +G+ ++ ++ Sbjct: 204 AGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQAREQGIKAVFMGPEGVGNKDITAI 263 Query: 266 AGAGLKDSEVVCAEAGGVTEAGKKPLEDFKAAYKKKFNQDVVIYAPYTYDALMTLADAMK 325 AG + +V A + L AA K+ N + A Y + +ADA+ Sbjct: 264 AGPA-SEGMLVTLPADFAADPANAALVKAFAAAKRDPNGPFQMPA---YSGVKIIADAIA 319 Query: 326 QAGSSDPKVYLPVLAKIKHKGVTGEIAFDAKGDILNGTLTLYTY 369 A S+DP+ + K G + +D KGD+ + ++T+ Sbjct: 320 GAKSTDPEKVAAYIHANSFKTPIGNVEYDKKGDLKSFKFVVFTW 363 >gi|300313247|ref|YP_003777339.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 142 bits (358), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 104/367 (28%), Positives = 174/367 (47%), Gaps = 27/367 (7%) Query: 13 LVAAFSFAGAAQAQEVIKIGHVAPLTGPNAHIGKDNENGARMAVDELNAKGFEIGGKK-- 70 L+ AF AG AQ ++ I +G L+ P I + GA++A+DE NA + K+ Sbjct: 15 LLWAFMQAGWAQERKTIALGFTGRLSEP---IAQSALQGAQLAIDEANA----LAAKRRS 67 Query: 71 -VTFQLVPQDDASDPKQATTVAQALVDAKVKGVVGHMNSGTTIPASKIYFDAGIPQISPS 129 + F+L+PQDD + A +A+ V KV GV+G +S + + IY A IPQI + Sbjct: 68 PLRFELLPQDDHGNENFAVNIARYYVKEKVAGVIGPWSSDAALATAAIYESAQIPQIGFT 127 Query: 130 ATNPKYTQQGFNTAFRVVANDGQLGGVLGRYAVNELKGKNVAVIDDRTAYGQGVAEEFRK 189 AT+ ++T G FRVV +L L A L+G+ + VI AY + +A+ + Sbjct: 128 ATSSQWTGMGNRMPFRVVGGTRELAATLAEVATRSLQGRRIFVIQSDAAYSRALADTLVE 187 Query: 190 SALAAGATIVATQYTT-DKATDFNAILTSVKSKKPDLVFFGGMDAVAGPMLRQMDQLGVA 248 + + I +Y ++TDF+A L + ++ + D++ F G+ A + ++ Sbjct: 188 NLGGSAQQIALARYQVGRRSTDFHAALKAAEAHQADVIVFVGLYPQARAFIDGANRTRTT 247 Query: 249 AKFMGGDGICTTELPSLAGAGLK--DSEVV---CAEAGGVTEAGKKPLEDFKAAYKKKFN 303 A+ + G LP+ L + E+ C + VT+ Y+++F Sbjct: 248 ARLLLTGGASNLSLPAPLELRLHVLEYEIARPHCPQWKVVTQ-----------TYQRRFG 296 Query: 304 QDVVIYAPYTYDALMTLADAMKQAGSSDPKVYLPVLAKIKHKGVTGEIAFDAKGDILNGT 363 Y+ Y YDA L A+ QAGS + L +++H G+ G ++FDA G + Sbjct: 297 AAPTTYSFYAYDAASVLTAAIVQAGSDNGDKIATALHRMRHAGLHGTLSFDATGANAHPR 356 Query: 364 LTLYTYK 370 TL Y+ Sbjct: 357 YTLLRYQ 363 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.132 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,154,578 Number of extensions: 50632 Number of successful extensions: 219 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 208 Number of HSP's successfully gapped: 17 Length of query: 381 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 294 Effective length of database: 1,207,925 Effective search space: 355129950 Effective search space used: 355129950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)