BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0201.p (153 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane... 100 4e-23 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 96 1e-21 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 96 2e-21 gi|300309360|ref|YP_003773452.1| hypothetical protein Hsero_0014... 96 2e-21 gi|300311036|ref|YP_003775128.1| signal transduction protein [He... 92 2e-20 >gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 797 Score = 100 bits (250), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 48/117 (41%), Positives = 71/117 (60%) Query: 1 MCNDPAVVRIHAKELKALGYTFSLDDFGTGFSGLSRLNTLPFDEIKIDASFISQIETNPK 60 + +DPA + + L +G+ S+DDFGTG+S L+ L LP DE+KID SF+ ++ + Sbjct: 662 IMDDPARAQNTLERLSNMGFALSIDDFGTGYSSLAYLKRLPVDELKIDKSFVMKMAQDQD 721 Query: 61 SLPLVRWMIALGSGLGMDGVAEGIETQAQLDLLLRTGCRLGQGYLFGRPIPAHCCLA 117 +VR + LG LG+ VAEG+E++ LL + GC GQGY G+P+P LA Sbjct: 722 DAKIVRSTVDLGHNLGLRVVAEGVESEEVWFLLRQMGCDQGQGYFMGKPMPVQHFLA 778 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 96.3 bits (238), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 46/108 (42%), Positives = 66/108 (61%) Query: 15 LKALGYTFSLDDFGTGFSGLSRLNTLPFDEIKIDASFISQIETNPKSLPLVRWMIALGSG 74 ++A G+T S+DDFGTG+S L+ L P + +KID SFI+ I NP S +V+ +IA+ Sbjct: 988 IRAEGFTISIDDFGTGYSSLAYLKRFPVNTLKIDKSFINDIPDNPNSSAIVQAIIAMSHA 1047 Query: 75 LGMDGVAEGIETQAQLDLLLRTGCRLGQGYLFGRPIPAHCCLALLSSQ 122 L + V EG+ET+ QL L G QGY F +P+P + LL+ Q Sbjct: 1048 LNLKVVTEGVETEKQLQALREFGSDEYQGYYFSKPVPYAQFVQLLAGQ 1095 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 95.5 bits (236), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 44/100 (44%), Positives = 59/100 (59%) Query: 15 LKALGYTFSLDDFGTGFSGLSRLNTLPFDEIKIDASFISQIETNPKSLPLVRWMIALGSG 74 LKA G FSLDDFGTG+S LS L LP +++KID SF+ + +P + + ++ L Sbjct: 922 LKAYGVEFSLDDFGTGYSSLSYLKRLPLNQLKIDQSFVRDVLIDPNDASIAKTIVNLAQS 981 Query: 75 LGMDGVAEGIETQAQLDLLLRTGCRLGQGYLFGRPIPAHC 114 LG+ +AEG+ET AQ L GC QGY F RP+ Sbjct: 982 LGLGVIAEGVETDAQRSFLADAGCHAYQGYFFCRPVTVEA 1021 >gi|300309360|ref|YP_003773452.1| hypothetical protein Hsero_0014 [Herbaspirillum seropedicae SmR1] Length = 726 Score = 95.5 bits (236), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 44/109 (40%), Positives = 67/109 (61%) Query: 13 KELKALGYTFSLDDFGTGFSGLSRLNTLPFDEIKIDASFISQIETNPKSLPLVRWMIALG 72 + L++ + SLDDFGTG+S LS L P D+IKID +F+ +++ + ++R +I LG Sbjct: 613 QALRSNEVSISLDDFGTGYSSLSYLGRFPIDQIKIDQTFVREMQNDAAMASIIRSIIMLG 672 Query: 73 SGLGMDGVAEGIETQAQLDLLLRTGCRLGQGYLFGRPIPAHCCLALLSS 121 L + +AEG+ET+ Q D L GC L QGY + +PIP LL++ Sbjct: 673 QSLNLSIIAEGVETEQQRDFLHAQGCLLYQGYWYSKPIPGEQFADLLNT 721 >gi|300311036|ref|YP_003775128.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 750 Score = 92.4 bits (228), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 44/111 (39%), Positives = 61/111 (54%) Query: 1 MCNDPAVVRIHAKELKALGYTFSLDDFGTGFSGLSRLNTLPFDEIKIDASFISQIETNPK 60 + +D VR L+A G +LDDFGTG+S +S L D +KID SF+ + Sbjct: 619 LLDDSEAVRSQLNNLRAAGIQVALDDFGTGYSAMSYLKKFSMDYLKIDRSFVQDMVDADS 678 Query: 61 SLPLVRWMIALGSGLGMDGVAEGIETQAQLDLLLRTGCRLGQGYLFGRPIP 111 + +V +I + LGM +AEG+ET Q LL GC QGYL+ RP+P Sbjct: 679 NKAIVEAIIVMAHRLGMKVIAEGVETMVQAQLLRDAGCEYVQGYLYSRPLP 729 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.139 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 541,216 Number of extensions: 22843 Number of successful extensions: 72 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 71 Number of HSP's successfully gapped: 18 Length of query: 153 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 76 Effective length of database: 1,255,275 Effective search space: 95400900 Effective search space used: 95400900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 126 (53.1 bits)