BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0199.p (718 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310609|ref|YP_003774701.1| outer membrane Iron(III) dicitr... 1242 0.0 gi|300311660|ref|YP_003775752.1| TonB-dependent receptor protein... 79 1e-15 gi|300310859|ref|YP_003774951.1| TonB-dependent siderophore rece... 62 1e-10 gi|300311652|ref|YP_003775744.1| TonB-dependent siderophore rece... 62 2e-10 gi|300309529|ref|YP_003773621.1| TonB dependent ferrisiderophore... 60 8e-10 >gi|300310609|ref|YP_003774701.1| outer membrane Iron(III) dicitrate transport protein [Herbaspirillum seropedicae SmR1] Length = 703 Score = 1242 bits (3213), Expect = 0.0, Method: Compositional matrix adjust. Identities = 588/703 (83%), Positives = 650/703 (92%) Query: 16 MKNNQAPFNHARDLRIATSTLASLVMLGFPLAGMAQQSAAPDGQSGLEPIQVSSDWLGTG 75 MK +QAP RDLRI T+ LASL +L FP+AG+AQQ++ DGQ GLEPIQVS DWLGTG Sbjct: 1 MKTHQAPSLFVRDLRITTTALASLAILAFPVAGLAQQASPSDGQPGLEPIQVSGDWLGTG 60 Query: 76 LESSVKTYPGARTVLKKQEIDSTGAVSIGEVMRRIPGVQSSDSAGTSGSAIALNIGVRGL 135 LESSVKTYPGARTV+KKQEI+STGAVSIG+ MRRIPGVQSSD++GT+GSAIALNIGVRGL Sbjct: 61 LESSVKTYPGARTVVKKQEIESTGAVSIGDAMRRIPGVQSSDNSGTAGSAIALNIGVRGL 120 Query: 136 TGRFSPRSTVLLDGIPLSVAPYGQPQLSFAPLSLNNIESIDVIRGGGAVRFGPQNVGGII 195 GR+SPRST+LLDGIP++VAPYGQPQLSFAPLSLNNIESIDV+RGGGAVRFGPQNVGGII Sbjct: 121 AGRYSPRSTILLDGIPMAVAPYGQPQLSFAPLSLNNIESIDVVRGGGAVRFGPQNVGGII 180 Query: 196 NFKTRSIPASEGISGDASVRYNSYGKGGGNAQYTAFAGGQSGNGLGMAFLYSGQDGSGWR 255 NFKTRSIP + GISGDA+VRYNSYG+GGGN QYTAFAGGQ+ NG GMAFLYSGQ+GSGWR Sbjct: 181 NFKTRSIPTTPGISGDAAVRYNSYGQGGGNTQYTAFAGGQAENGFGMAFLYSGQEGSGWR 240 Query: 256 DKSDEHLNDFALKMRYEISPRSELHAKFNYYDVLSRTPGGLTTAQYAADPFQNTRQRDNW 315 DKSDEHLNDFALKMRYEISP SEL+AK NYYDVLSRTPGGL+ AQYAADPFQ+TRQRDNW Sbjct: 241 DKSDEHLNDFALKMRYEISPTSELYAKINYYDVLSRTPGGLSVAQYAADPFQSTRQRDNW 300 Query: 316 SGTRKGFDVGYLNTLSDHQELEIRTYFNQATRQSSLINVTGVRLSHQPRNYETLGVEPRY 375 GTRKGFDVGYLNT+SD QE EIRTYF+QATRQS+LIN G RLSHQPRNYETLG+EPRY Sbjct: 301 GGTRKGFDVGYLNTISDRQEFEIRTYFSQATRQSTLINPAGTRLSHQPRNYETLGIEPRY 360 Query: 376 TQRFAWGGTTHDVTVGYRYIRERGDDNIYNEAVRTGVLSGFTQFQNSTDAHAGYIDDKIA 435 TQRFAWGGTTHDVTVGYRY+RERGDDN+Y+EAV TGVLSGFTQFQNSTDAHAGYIDDKIA Sbjct: 361 TQRFAWGGTTHDVTVGYRYVRERGDDNVYSEAVLTGVLSGFTQFQNSTDAHAGYIDDKIA 420 Query: 436 FGKWRITPGLRFEQVRSSRYEMGKKDPYEVANDKLLPSINLAYLLTEQLTLFSNYNTSFG 495 FGKWRITPGLRFE V+S+RY++ Y+V+N+K LPSIN+AYLLTEQLTLFSNYNTSFG Sbjct: 421 FGKWRITPGLRFEHVQSTRYDVANNASYQVSNNKPLPSINVAYLLTEQLTLFSNYNTSFG 480 Query: 496 VVQNSQLNTMSSANPLSPELARTMEGGMRWKSAQASAEATVFHINFDNQIVSVQGTTPNV 555 VVQNSQLNTMSSANPLSPELA+T+EGG+RWKSA+ASAEATVFHI+FDNQIVSVQGT P+V Sbjct: 481 VVQNSQLNTMSSANPLSPELAKTIEGGVRWKSARASAEATVFHIDFDNQIVSVQGTNPSV 540 Query: 556 FRNIGRTQHDGVELAMDYAFAPDSLLKGWSVFANYTYTRALQKSGSNPGKDLPFYARTTD 615 FRNIG+TQHDG+ELAMDYAF S+LKGWSV+ANYTYTRALQKSG+NPGKDLPFYARTTD Sbjct: 541 FRNIGKTQHDGIELAMDYAFDQQSVLKGWSVYANYTYTRALQKSGANPGKDLPFYARTTD 600 Query: 616 TLGVRMQAGPWTLNLSTTHQGRQFADEANTVAESADGSIGEIPGVRLWNAQASWKVPGKK 675 T+G R ++GPW NLSTTHQGRQF+DEANT+AESADGSIGEIPG RLWNAQ WKVPG K Sbjct: 601 TVGTRFKSGPWGFNLSTTHQGRQFSDEANTIAESADGSIGEIPGFRLWNAQVDWKVPGTK 660 Query: 676 GFDLTAGVNNLLDRRYFTRTVDGNLGKLVGAPRTLYIQGRYAF 718 GFD+ AG+NNL DRRYFTRT D NLGKLVGAPRT+Y+QGRY+F Sbjct: 661 GFDVFAGINNLTDRRYFTRTTDNNLGKLVGAPRTVYVQGRYSF 703 >gi|300311660|ref|YP_003775752.1| TonB-dependent receptor protein [Herbaspirillum seropedicae SmR1] Length = 705 Score = 79.3 bits (194), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 131/559 (23%), Positives = 220/559 (39%), Gaps = 70/559 (12%) Query: 73 GTGLESSVKTYPGARTVLKKQEIDSTGAVSIGEVMRRIPGVQSSDSAGTSGSAIALNIGV 132 T ++ V P + +V+ + E+D+ G S+ +V+R +PG +S++ G + Sbjct: 58 ATKTDTPVLEVPQSVSVVTRDEMDARGVTSLLDVLRYMPGA-NSETHGVDPRGYEY-FNL 115 Query: 133 RGLTGRFSPRSTVLLDGIPLSVAPYGQPQLSFAPLSLNNIESIDVIRGGGAVRFGPQNVG 192 RG + ++ L+G+ +GQ + +E I+V+RG G+V FG + G Sbjct: 116 RGFIN--AQVTSNFLNGLRQIPGGFGQFRTE-----TYGMERIEVLRGPGSVTFGQADPG 168 Query: 193 GIINFKTR----SIPASEGISGDASVRYNSYGKGGGNAQYTAFAGGQSGNGLGMAFLYSG 248 G+IN ++ IP G+ + R G+ Q + G GL Y Sbjct: 169 GVINRVSKLPGMDIPNELGVDIGSFNRKQVVADLNGHTQDGEYLWRVVGLGLDSNNQYKF 228 Query: 249 QDGSGWRDKSDEHLNDFALKMRYEISPRSELHAKFNYYDVLSRT-------PGGLTTAQY 301 +G D + A + + S + L +Y + S + PG + T Sbjct: 229 NNGQ----SGDNNRFYLAPSLTWRPSASTSLTLMADYMNDRSGSSRWTAVRPGNVLTHTL 284 Query: 302 AADPFQNTRQRDNWSGTRKGFDVGYLNTLSDHQELEIRTYFNQATRQS--SLIN---VTG 356 DP + + D WS GY R F QA +S + +N +TG Sbjct: 285 IGDPNFDRQSNDQWS-------FGYQFEHKFDNAWTFRQNFRQANLKSVYAALNPGTLTG 337 Query: 357 VRLSHQPRNY----ETLGVEPRYTQRFAWGGTTHDVTVGY-------RYIRERG------ 399 L+ Y + V+ + R WG T H V G R +R RG Sbjct: 338 SVLTRGTTVYNSQVDNTLVDNQLEGRLKWGATEHTVLFGLDWSRMYDREVRYRGAAPSLN 397 Query: 400 -DDNIYNEAVRTGVLSGFTQFQNSTDAHAGYIDDKIAFG-KWRITPGLRFEQVRSSRYEM 457 D+ +Y + + F + + + Y D+I F + +T G R+++V+ Sbjct: 398 IDNPVYGVTI-PAATTLFGNLEENMNQTGLYAQDQIKFDQRLTLTLGGRWDKVKDETRNY 456 Query: 458 GKKDPYEVANDKLLPSINLAYLLTEQLTLFSNYNTSFGVVQNSQLNTMSSANPLSPELAR 517 + + L I L+YL+T + + +Y TSF S N P P AR Sbjct: 457 FNRTTTRSDDSALSGRIGLSYLVTRDIAPYVSYATSFLPQTGSDFN----GKPFDPTKAR 512 Query: 518 TMEGGMRWKSAQASA--EATVFHINFDN-QIVSVQGTTPNVFRNIGRTQHDGVELAMDYA 574 E G++++ A A +F + N Q T NV G + G+EL Sbjct: 513 QAEVGIKYQPADGKTLFTAALFELTKTNVQTPDPAHTNFNVLS--GEIKSRGLELE---- 566 Query: 575 FAPDSLLKGWSVFANYTYT 593 A ++KG ++ A+YTYT Sbjct: 567 -AKGEIVKGLNLSASYTYT 584 >gi|300310859|ref|YP_003774951.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 705 Score = 62.4 bits (150), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 69/275 (25%), Positives = 115/275 (41%), Gaps = 31/275 (11%) Query: 429 YIDDKIAFG-KWRITPGLRFEQVRSSRYEMGKKDPYEVANDKLLPSINLAYLLTEQLTLF 487 Y+ D+++ +WR+ G+R + RS+ + P + L YLLT +++ Sbjct: 428 YLQDQLSLSERWRLLAGVRADAFRSTLNNLRNGAVTRQDKHAYSPRLGLTYLLTPSWSVY 487 Query: 488 SNYNTSFGVVQNSQLNTMSSANPLSPELARTMEGGMRWKSAQASAEAT--VFHINFDNQI 545 + + SF S + S N SPE +R E G++++S + AT VF I N + Sbjct: 488 VSASKSFRPNSGSDV----SGNAFSPETSRAAEAGLKFQSEDKRSGATLAVFDITKQN-V 542 Query: 546 VSVQGTTPNVFRNIGRTQHDGVELAMDYAFAPDSLLKGWSVFANYTYTRA-LQKSGSNP- 603 ++ PN G + GVEL A L W V N YT A + + + P Sbjct: 543 LTTDPANPNFSIAAGEVRSKGVELD-----ANARLGHHWRVTGNLAYTDAYVSRDNTLPV 597 Query: 604 GKDLPFYARTTDTL-----GVRMQAGPWTLNLSTTHQGRQFADEANTVAESADGSIGEIP 658 G L R++ +L P+ + + GR+ D+A + +P Sbjct: 598 GSRLVNIPRSSASLLAVFEDADAHGAPYGVGGGLNYVGRRTGDQAGSFM---------LP 648 Query: 659 GVRLWNAQASWKVPGKKGFDLTAGVNNLLDRRYFT 693 A A W+ K L+ VNNL ++RY++ Sbjct: 649 SYVTARALAYWQFTPK--MKLSLDVNNLFNKRYYS 681 >gi|300311652|ref|YP_003775744.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 832 Score = 61.6 bits (148), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 143/678 (21%), Positives = 257/678 (37%), Gaps = 96/678 (14%) Query: 88 TVLKKQEIDSTGAVSIGEVMRRIPGVQSSDSAGTSGSAIALNIGVRGLTGRFSPRSTVLL 147 +V+ + E+++ GA SI +V+R +PG + N+ RG + ++ L Sbjct: 200 SVVTRAEMEARGANSILDVLRYMPGANTETHGVDPRGYDYFNL--RGFIN--AQTTSNYL 255 Query: 148 DGIPLSVAPYGQPQLSFAPLSLNNIESIDVIRGGGAVRFGPQNVGGIINFKTRSIPASEG 207 +G+ + +G + +E ++V+RG + FG + GG++N ++ A G Sbjct: 256 NGLRQIPSGFGMFRTE-----TYGLERVEVLRGANSASFGQADPGGVVNRVSKL--AGSG 308 Query: 208 ISGDASVRYNSYGKGGGNAQYTAFAGGQSGNGLGMA----FLYSGQDGSGWRDKSDEHLN 263 + V S+ + A +G G A L G + + + Sbjct: 309 ARNELMVDVGSFQRRQVAAD---LSGDLDPEGRVQARLVGLLLDGDTQFRYANGRAGDND 365 Query: 264 DFALKMRYEISPRSELHAKFNYYDVLSRTPGGLTTAQYA---------ADPFQNTRQRDN 314 L +I P ++ + R+ G TA A DP + ++ + Sbjct: 366 RLYLAPSLKIRPSADTSLILLAEYLKDRSGSGRWTAVRADGSQTHTLLGDPDFDQQRGEQ 425 Query: 315 WSGTRKGFDVGYLNTLSDHQELEIRTYFNQATRQSSLINVT-----GVRLSHQPRNYET- 368 WS +G++ +R +F QA +S V G LS Y+T Sbjct: 426 WS-------LGWMLEHRLDATWTLRQHFRQAALRSQYAAVNPGSFNGSILSRSTAVYDTQ 478 Query: 369 ---LGVEPRYTQRFAWGGTTHDVTVGY-------RYIRERG-------DDNIYNEAVRTG 411 ++ + R WG H V +G R R RG D+ +Y + + Sbjct: 479 VENTLLDNQALARVQWGQAEHQVLLGLDWSHMSAREQRYRGVAPSLDIDNPVYTQPI-PA 537 Query: 412 VLSGFTQFQNSTDAHAGYIDDKIAFGKWRITPGLRFEQVRSSRYEMGKKDPYEVANDKLL 471 + F + Y D+I + + +T G R+++ R S + Sbjct: 538 ATTRFGDLDQTLVQTGLYAQDQIRYQRIVLTLGARYDRSRDSTRNAANNSLQAAEENAFS 597 Query: 472 PSINLAYLLTEQLTLFSNYNTSFGVVQNSQLNTMSSANPLSPELARTMEGGMRWKSAQAS 531 + L+YL++ +L + +Y +SF Q P AR +E G++++ Sbjct: 598 GRVGLSYLVSPELAPYVSYGSSF----LPQAGQDVDGKPFKAATARQIEAGVKYQPGHGR 653 Query: 532 A--EATVFHINFDNQIVSVQGTTP---NVFRNIGRTQHDGVELAMDYAFAPDSLLKGWSV 586 A A ++ + DN + G+ P N + G+ + GVEL A LL G +V Sbjct: 654 AVYTAALYQLVKDNAL----GSDPLHANFSVSNGQVRSRGVELE-----AKGELLPGLNV 704 Query: 587 FANYTYTRALQKSGSNP---GKDLPFYARTTDTLGV--RMQAGPWT---LNLSTTHQGRQ 638 A YTY + + + P GK R +L + +Q G + + GR Sbjct: 705 TAAYTYAQVVNTQNAAPELIGKTPILVPRQAASLWLDYTVQRGDLAGMGVGAGARYTGRN 764 Query: 639 FADEANTVAESADGSIGEIPGVRLWNAQASWKVPGKKGFDLTAGVNNLLDRRYFTRTVDG 698 +A ANTV +A + + VR+ + P + F+ V+NL +R+Y + + Sbjct: 765 YATAANTVENAAQVILDAM--VRI------DRGPWRYAFN----VSNLANRQYTSCLAEP 812 Query: 699 NLGKLVGAPRTLYIQGRY 716 L RT + RY Sbjct: 813 TLTCFWAPERTAVLSARY 830 >gi|300309529|ref|YP_003773621.1| TonB dependent ferrisiderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 704 Score = 59.7 bits (143), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 77/320 (24%), Positives = 131/320 (40%), Gaps = 59/320 (18%) Query: 424 DAHAGYIDDKIAFG-KWRITPGLRFEQVRS----------SRYEMGKKDPYEVANDKLL- 471 D A Y+ D ++ +W+ GLR+++ R S ++ + D L+ Sbjct: 415 DTFATYLQDSLSLSEQWKALVGLRYDRYRQQSRILNLANGSTADLQRTDTAVSPRAGLVW 474 Query: 472 -PSINLAYLLTEQLTLFSNYNTSFGVVQNSQLNTMSSANPLSPELARTMEGGMRWK--SA 528 PS+ +Y L+ + F SF + N+ L+PE R E G ++ Sbjct: 475 QPSLTQSYYLSWSRS-FQPSGESFALATNNA--------DLAPEQTRNTEAGAKYDFWEG 525 Query: 529 QASAEATVFHINFDNQIVSVQGTTPN-VFRNIGRTQHDGVELAMDYAFAPDSLLKGWSVF 587 +ASA +VF + DN ++ PN V +G+ + DG EL+ F+ D L +GW + Sbjct: 526 RASATVSVFRLERDN----IKSALPNGVVIPVGKQRTDGAELS----FSAD-LKQGWKLL 576 Query: 588 ANYTYTRALQKSGSNPGKDLPFYARTTDTLGVRMQAGPWTLNLSTTHQ---------GRQ 638 A Y + A+ S+ +PF + TL R W G + Sbjct: 577 AGYAWLNAVITESSSFDNGVPFKGKNA-TLTPRHSGNLWLTRELGEGLVVGGGANAVGAR 635 Query: 639 FADEANTVAESADGSIGEIPGVRLWNAQASWKVPGKKGFDLTAGVNNLLDRRYFT--RTV 696 FA+ NTV +PG +A A+++ +++ + NL + Y Sbjct: 636 FANPGNTVT---------LPGYVTVDAMAAYRT---SKYEVQLNIGNLFNTGYIISGHGT 683 Query: 697 DGNLGKLVGAPRTLYIQGRY 716 + NL + GAPR + + RY Sbjct: 684 NPNL-SMPGAPRNVALTLRY 702 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,404,329 Number of extensions: 106067 Number of successful extensions: 344 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 347 Number of HSP's successfully gapped: 12 Length of query: 718 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 626 Effective length of database: 1,184,250 Effective search space: 741340500 Effective search space used: 741340500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)