BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0187.p (464 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 780 0.0 gi|300311185|ref|YP_003775277.1| major facilitator superfamily p... 148 1e-36 gi|300312966|ref|YP_003777058.1| major facilitator superfamily p... 142 6e-35 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 132 5e-32 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 121 1e-28 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 780 bits (2015), Expect = 0.0, Method: Compositional matrix adjust. Identities = 415/454 (91%), Positives = 432/454 (95%) Query: 11 PHAGMLPLVALAIGFVMAMLDVTVVNVGLSNISTNLDAPLSMLVWIVDGYTLTFAAMLLV 70 P AGMLPLVALAIGFVMAMLDVTVVNVGLSNI+ +LDAPLS LVWIVDGYTLTFAAMLLV Sbjct: 25 PRAGMLPLVALAIGFVMAMLDVTVVNVGLSNIAGSLDAPLSTLVWIVDGYTLTFAAMLLV 84 Query: 71 GGALADRFGARRLYLTGLFLFVVASLLCGAAPNGPFLIAARLLQGLGAAFFMPSSLSLLT 130 GGALADRFGARRLYLTGLFLFV+ASLLCGAAPNGPFLI ARLLQGLGAAFFMPSSLSLLT Sbjct: 85 GGALADRFGARRLYLTGLFLFVLASLLCGAAPNGPFLIVARLLQGLGAAFFMPSSLSLLT 144 Query: 131 HVYEDDRVRARMLGVWSATVGLAAAAGPLLGGVLIHWLGWRSVFLINVPVGLLGLLMARK 190 HVYEDDRVRARMLGVWSATVGLAAA GPL+GGVLIHWLGWRSVFLINVPVGL+GL+MARK Sbjct: 145 HVYEDDRVRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGLVMARK 204 Query: 191 LIPQVAGHARALHLFSHLAGVVMLAGISFVLIQGPVYGWASPRILAALAVAVLAAVALVR 250 LIPQV GHARAL+L SHLAGVVMLAG+SFVLIQGPVYGW SPRILA LAVA+ A AL+R Sbjct: 205 LIPQVGGHARALNLSSHLAGVVMLAGLSFVLIQGPVYGWTSPRILAGLAVALSAGAALIR 264 Query: 251 HELRGIAPLLPKALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGRGADALQTGLQL 310 HELRGIAPLLPKALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGRGADALQTGLQL Sbjct: 265 HELRGIAPLLPKALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGRGADALQTGLQL 324 Query: 311 VPMMAVVFFGNLMSGRMAAHWGPRVPLLLGLSVGTVFTAVAMSFRPETPYWLLALVCAAA 370 VPMMAV+FFGNLMSGRM+AHWGPRVPLLLGLSVGTVFTA+AM F P TPYWLLALVCA+A Sbjct: 325 VPMMAVIFFGNLMSGRMSAHWGPRVPLLLGLSVGTVFTALAMGFSPATPYWLLALVCASA 384 Query: 371 NLGISTAIPSMTTVVMQVAGKAHANSAAAALNANRQIGALVGVALMGAILHSVAGWSVRN 430 NLGISTAIP+MTTVVMQVAGKAHANSAAAALNANRQIGALVGVALMGAILHSV GW++RN Sbjct: 385 NLGISTAIPAMTTVVMQVAGKAHANSAAAALNANRQIGALVGVALMGAILHSVTGWNLRN 444 Query: 431 PLAFGVMALAYGVALWLVWRFVGVVKEQWVTRRA 464 PLAFGVMA Y ALWLVWRFVGVVKEQW RRA Sbjct: 445 PLAFGVMAAGYASALWLVWRFVGVVKEQWQVRRA 478 >gi|300311185|ref|YP_003775277.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 532 Score = 148 bits (373), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 124/427 (29%), Positives = 198/427 (46%), Gaps = 19/427 (4%) Query: 18 LVALAIGFVMAM--LDVTVVNVGLSNISTNLDAPLSMLVWIVDGYTLTFAAMLLVGGALA 75 L L I FV+ + LD T+V L I L + W+ Y LT + + G L Sbjct: 22 LATLGICFVIMLVALDQTIVGTALPTIVAELKG-FELYAWVATAYLLTSVITVPIFGRLG 80 Query: 76 DRFGARRLYLTGLFLFVVASLLCGAAPNGPFLIAARLLQGLGAAFFMPSSLSLLTHVYED 135 D FG + + + +FV AS LCG A + FL+ AR LQG+G + + + + ++ D Sbjct: 81 DYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQGIGGGMLVGTCFASVADLFPD 140 Query: 136 DRVRARMLGVWSATVGLAAAAGPLLGGVLIHWLGWRSVFLINVPVGLLGLLMARKLIPQV 195 RVR R + S + G+A A GP LGG L LGWR VF N+P+G++ L + +P + Sbjct: 141 PRVRLRWQIILSVSFGIATAVGPSLGGFLTQGLGWRWVFYCNLPIGVISLFFVWRFVPHI 200 Query: 196 A--GHARALHL------FSHLAGVVMLAGISFVLIQGPVYGWASPRILAALAVAVLAAVA 247 HA + L L+ + + + +G G +L LAV+V A A Sbjct: 201 RHMQHAGKMRLDWPGALLISLSLGSLQLLVELLPKRGVTSG-----MLVLLAVSVAAFYA 255 Query: 248 LVRHELRGIAPLLPKALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGRGADALQTG 307 L + E R P+LP + A+ + L F ++ L Y L Q G G + G Sbjct: 256 LWKWEQRAAQPILPFEMLRDRALASLFLLSVLAGFAMFSMLMYAPLLFQGGFGLSPREAG 315 Query: 308 LQLVPMMAVVFFGNLMSGRMAAHW-GPRVPLLLGLS-VGTVFTAVAMSFRPETPYWLLAL 365 L + P++A + ++++GR+ P + + LG + + + + R +LLA Sbjct: 316 LLITPLVACITVASIVNGRIITRIPNPNLMMNLGFALIAASCLGIVLCDRVTGKGFLLA- 374 Query: 366 VCAAANLGISTAIPSMTTVVMQVAGKAHANSAAAALNANRQIGALVGVALMGAILHSVAG 425 V A G+ +P++T Q A + H A A L + R IG ++G A+ G ++ + G Sbjct: 375 VMLAGGFGLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGMLGTAITGTLVSQMYG 434 Query: 426 WSVRNPL 432 V L Sbjct: 435 AGVEKAL 441 >gi|300312966|ref|YP_003777058.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 544 Score = 142 bits (358), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 128/410 (31%), Positives = 187/410 (45%), Gaps = 1/410 (0%) Query: 22 AIGFVMAMLDVTVVNVGLSNISTNLDAPLSMLVWIVDGYTLTFAAMLLVGGALADRFGAR 81 + G M +LD VV + L ++ +L A S + W+V Y L FAA+LL GA ADR+G R Sbjct: 42 SFGCAMTVLDTNVVGIILPTVARDLGASFSQIEWVVSSYVLCFAALLLPAGATADRYGRR 101 Query: 82 RLYLTGLFLFVVASLLCGAAPNGPFLIAARLLQGLGAAFFMPSSLSLLTHVYEDDRVRAR 141 R+ L G+ LF + SL C AP+ L AR LQG+GAAF + +L+++ H + D+ R R Sbjct: 102 RVLLGGIALFALTSLACALAPSAQALYGARALQGVGAAFLLAPALAVIGHAFHDEDQRER 161 Query: 142 MLGVWSATVGLAAAAGPLLGGVLIHWLGWRSVFLINVPVGLLGLLMARKLIPQVAGH-AR 200 +W +GL PL+GGV+ LGWR F +N+P LL + + +P+ R Sbjct: 162 AWALWGGIMGLTMVLAPLIGGVVNTLLGWRWAFAVNLPACLLLAVAVWRHVPESRNPLPR 221 Query: 201 ALHLFSHLAGVVMLAGISFVLIQGPVYGWASPRILAALAVAVLAAVALVRHELRGIAPLL 260 L L+ + +++ LI GP +GW S +A L V + + R P+L Sbjct: 222 PLDPLGILSSSSAVFLLTWALITGPEHGWTSVACVARGLGGALLLVLFIAWQRRAAHPML 281 Query: 261 PKALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGRGADALQTGLQLVPMMAVVFFG 320 +LF F AA F L LFLQ RG A QTGL ++P + Sbjct: 282 ELSLFRHAGFVAAVAAMFAYAASAQVMASLLPLFLQNARGLSAWQTGLAMLPFALAMLLL 341 Query: 321 NLMSGRMAAHWGPRVPLLLGLSVGTVFTAVAMSFRPETPYWLLALVCAAANLGISTAIPS 380 + R+A L LGL V + + + AL A G Sbjct: 342 PQVGSRLARWLSSPQMLALGLGVTALGNLLMIQAALSGAAAWTALGMAVLGSGGGLLNGQ 401 Query: 381 MTTVVMQVAGKAHANSAAAALNANRQIGALVGVALMGAILHSVAGWSVRN 430 +M + A A+ R G L G A +GA+L A +++N Sbjct: 402 TQKAIMGNVPRERAGMASGISTTARFTGVLAGFAGLGAVLADTARHAMQN 451 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 132 bits (333), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 106/314 (33%), Positives = 155/314 (49%), Gaps = 9/314 (2%) Query: 4 PPLADAAPHAGMLPLVALAIGFVMAMLDVTVVNVGLSNISTNLDAPLSMLVWIVDGYTLT 63 PPL A G L ++++ M +LD ++ NV + IS NL + W++ + Sbjct: 15 PPLTGANLVLGTL---SVSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAA 71 Query: 64 FAAMLLVGGALADRFGARRLYLTGLFLFVVASLLCGAAPNGPFLIAARLLQGLGAAFFMP 123 A + + G L R GA RL++T + LFV+AS LCG AP P L+AAR+LQGL A +P Sbjct: 72 NAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVP 131 Query: 124 SSLSLLTHVYEDDRVRARMLGVWSATVGLAAAAGPLLGGVLIHWLGWRSVFLINVPVGLL 183 S SLL Y + + L +W T +A AGP LGG + W +F IN+PVGL+ Sbjct: 132 LSQSLLLGAYPKSK-SSFALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLI 190 Query: 184 --GLLMARKLIPQVAGHARALHLFSHLAGVVMLAGISFVLIQGPVYGW-ASPRILAALAV 240 G+ +A + H + V +A +L +G W ASP I+ + Sbjct: 191 AAGVTLAIYRSRETPTHKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGII 250 Query: 241 AVLAAVALVRHELRGIAPLLPKALFATPQFAAANGIGFLINFGV-YGNLFYLALFLQQGR 299 A++A V EL P++ LF FA I I +GV +GNL L +LQQ Sbjct: 251 ALIAFAYFVVWELNNDHPVVDLRLFGRRNFAGGT-IAISIGYGVFFGNLVILPQWLQQYL 309 Query: 300 GADALQTGLQLVPM 313 G ++ +GL P+ Sbjct: 310 GYRSIDSGLSTAPI 323 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 121 bits (303), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 131/419 (31%), Positives = 192/419 (45%), Gaps = 37/419 (8%) Query: 21 LAIGFVMAMLDVTVVNVGLSNISTNLDAPLSMLVWIVDGYTLTFAAMLLVGGALADRFGA 80 +A GF M LD T+VN L ++ +L P+ + +V YTLT A++ G LADRFG Sbjct: 58 VASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFGT 117 Query: 81 RRLYLTGLFLFVVASLLCGAAPNGPFLIAARLLQGLGAAFFMP-SSLSLLTHVYEDDRVR 139 RR+Y + LF + SLLC A + P L+AAR++QGLG + +P L++L V + Sbjct: 118 RRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIA 177 Query: 140 ARMLGVWSATVGLAAAAGPLLGGVLIHWLGWRSVFLINVPVGLLGLLMARKLIPQ--VAG 197 A L S L GP+LGG L+ L W +FLIN+PVG+LG+ +P +AG Sbjct: 178 A--LASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLAG 235 Query: 198 HARALHLFSHLAGVVMLAG--ISFVL-----IQGPVYGWASPRILAALAVAVLAAVALVR 250 R L G V+L+ ++F L GW+ +A VA L + R Sbjct: 236 TPRF-----DLLGCVLLSACMVAFSLALDVPFDSHRTGWS----IALFVVAALTVLGYWR 286 Query: 251 HELRGIAPLLPKALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGRGADALQTGLQL 310 H PL LF P F+ + G F L L LQ G L G+ L Sbjct: 287 HASVHPRPLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVL 346 Query: 311 VPMMAVVFFGNLMSGRMAAHWGPRVPLLLG----LSVGTVFTAVA-MSFRPETPYW-LLA 364 +P ++G +A W + G L V T A MSF TP W L A Sbjct: 347 LPAA--------IAGTIAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTA 398 Query: 365 LVCAAANLGISTAIP--SMTTVVMQVAGKAHANSAAAALNANRQIGALVGVALMGAILH 421 + A G S ++ +M +V ++ K A S + + + + +GV + G +++ Sbjct: 399 AIVQLAIFGASNSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVN 457 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.140 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,416,432 Number of extensions: 57623 Number of successful extensions: 494 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 471 Number of HSP's successfully gapped: 21 Length of query: 464 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 375 Effective length of database: 1,198,455 Effective search space: 449420625 Effective search space used: 449420625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)