BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0175.p (454 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310313|ref|YP_003774405.1| acyl-CoA dehydrogenase [Herbasp... 670 0.0 gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbasp... 109 5e-25 gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 105 7e-24 gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbasp... 103 2e-23 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 86 8e-18 >gi|300310313|ref|YP_003774405.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 550 Score = 670 bits (1729), Expect = 0.0, Method: Compositional matrix adjust. Identities = 334/365 (91%), Positives = 349/365 (95%) Query: 1 MTFAAIPVLQQEPALFEQLRSRLFSREHDGRDLPITQKRSILIGMGMTEKQGGSDVRSNA 60 MTFAAI VLQQEPALFEQLR+RLFSREHD RDLPI QKRSILIGMGMTEKQGGSDVRSNA Sbjct: 134 MTFAAIAVLQQEPALFEQLRTRLFSREHDARDLPIPQKRSILIGMGMTEKQGGSDVRSNA 193 Query: 61 SIAMPADPLEDGRGKAYLLSGHKWFFSAPMCDAHLMLARTENGLSCFFVPRWLPDGTRNP 120 SIAMP DPL DGRG AYLLSGHKWFFSAPMCDAHLMLARTENGLSCFFVPRWLPDGTRNP Sbjct: 194 SIAMPVDPLNDGRGAAYLLSGHKWFFSAPMCDAHLMLARTENGLSCFFVPRWLPDGTRNP 253 Query: 121 ILIQRLKDKLGNRSNSSSEVEFEEAMGIMVGEDGRGIPTIIEMANHTRLDCIIGSAALMR 180 ILIQRLKDKLGNRSNSSSEVEFEEAMGIMVG++GRGIPTIIEMANHTRLDCIIGSAALMR Sbjct: 254 ILIQRLKDKLGNRSNSSSEVEFEEAMGIMVGDEGRGIPTIIEMANHTRLDCIIGSAALMR 313 Query: 181 QALVQALHHARHRSAFGRRLAEQPIMRAVLADLALESEAATLLMLRVAHAFDAPDDPLQR 240 QALVQA+HHARHRSAFGRRLAEQP+MRAVLADLALESEAAT+LMLRVAHAFDAPDDPLQR Sbjct: 314 QALVQAIHHARHRSAFGRRLAEQPLMRAVLADLALESEAATMLMLRVAHAFDAPDDPLQR 373 Query: 241 AWKRIVTPAAKFWICKRALEFTGECMEVWGGNGYVETGPMARLYREAPVNSIWEGSGNVM 300 AWKRI+TPAAKFWICKR LEFTGECMEVWGGNGYVET PMARLYREAPVNSIWEGSGNVM Sbjct: 374 AWKRIITPAAKFWICKRTLEFTGECMEVWGGNGYVETAPMARLYREAPVNSIWEGSGNVM 433 Query: 301 CLDVLRAISRETEGFAQLLIALDDAASTHPALAQRVRSLKQMLSAAEEEREACARRLVQQ 360 CLDVLRA++RET+G A LL+ LDDAA+ HPAL Q+V +LKQML+A EEEREA ARRLVQQ Sbjct: 434 CLDVLRAMARETQGLALLLLELDDAAAGHPALRQQVDALKQMLAAPEEEREAGARRLVQQ 493 Query: 361 LVLAL 365 LVLAL Sbjct: 494 LVLAL 498 >gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 109 bits (272), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 83/296 (28%), Positives = 145/296 (48%), Gaps = 41/296 (13%) Query: 45 MGMTEKQGGSDVRSNASIAMPADPLEDGRGKAYLLSGHKWFFSAPMCDA-----HLMLAR 99 M +TE G+D+ + A+P DG +Y ++G+K F SA D HL+LAR Sbjct: 165 MCLTEAHCGTDL---GLLRSKAEPQADG---SYKITGNKIFISAGENDVSANIIHLVLAR 218 Query: 100 TEN------GLSCFFVPRWLPD-----GTRNPILIQRLKDKLGNRSNSSSEVEFEEAMGI 148 + G+S F VP++LP+ G RNPI +++K+G N++ ++ + A G Sbjct: 219 LPDAPEGSKGISLFLVPKFLPNADGSLGARNPIFCGAIEEKMGIHGNATCQMNLDGATGW 278 Query: 149 MVGEDGRGIPTIIEMANHTRLDCIIGSAALMRQALVQALHHARHR----SAFGRRLAEQP 204 ++G+ +G+ + N RL + L A AL +A+ R S G + A++P Sbjct: 279 LIGQPHKGLQAMFVFMNAARLGVGMQGLGLTEVAYQNALVYAKDRLQMRSLSGIKAADKP 338 Query: 205 ----IMRAVLADLALESEA--------ATLLMLRVAHAFDAPDDPLQRAWKRIV---TPA 249 I+ + + L ++A + + L + PD+ +++ IV TP Sbjct: 339 ADPIIVHPDVRRMLLTAKAFAEGGRAFTSYVALELDKELTHPDEQVRKDSADIVALLTPV 398 Query: 250 AKFWICKRALEFTGECMEVWGGNGYVETGPMARLYREAPVNSIWEGSGNVMCLDVL 305 K ++ A T EC++V+GG+GY+ M + R+A +N I+EG+ + LD+L Sbjct: 399 VKAFLTDNAWMATSECLQVYGGHGYIAEWGMEQYVRDARINMIYEGTNTIQSLDLL 454 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 105 bits (262), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 87/289 (30%), Positives = 134/289 (46%), Gaps = 31/289 (10%) Query: 42 LIGMGMTEKQGGSDVRSNASIAMPADPLEDGRGKAYLLSGHKWFFSAPMCD-----AHLM 96 L M +TE Q GSD+ + A+P D DG +Y LSG K F S D HL+ Sbjct: 161 LATMCLTEAQAGSDLGQLRTRAVPQD---DG---SYRLSGDKIFISGGEHDLTPNIVHLV 214 Query: 97 LARTEN------GLSCFFVPRWLPDGTRNPILIQRLKDKLGNRSNSSSEVEFEEAMGIMV 150 LAR + GLS F PR L DG+RN + ++ K+G +++ + FE+A +V Sbjct: 215 LARLPDAPAGSKGLSLFLAPRVLDDGSRNGVHCTGIEHKMGIHGSATCSLHFEQAQAWLV 274 Query: 151 GEDGRGIPTIIEMANHTRLDCIIGSAALMRQALVQALHHARHR-------SAFGRRLAEQ 203 GE GRG+ + M N RL L A +A +AR R S+ + Sbjct: 275 GEAGRGLAAMFVMMNAARLHVGAQGLGLAEAAYQRASAYARERRQSRAPGSSGNDLIVHH 334 Query: 204 PIMRAVLADLALESEAATLLMLRVAHAFD-APDDPL----QRAWKRI--VTPAAKFWICK 256 P ++ +L + E A LL D A DP R +R+ +TP K ++ Sbjct: 335 PAVQKLLMEQRCRIEGARLLTCWAGLLLDLAEHDPEAALRSRHHERLEFITPVIKAFLTD 394 Query: 257 RALEFTGECMEVWGGNGYVETGPMARLYREAPVNSIWEGSGNVMCLDVL 305 + + ++V+GG+GYV + + R+A + I+EG+ + +D L Sbjct: 395 QGFQCASRALQVFGGHGYVVETGIEQFMRDARITMIYEGTNEIQAIDFL 443 >gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 103 bits (258), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 94/313 (30%), Positives = 152/313 (48%), Gaps = 43/313 (13%) Query: 45 MGMTEKQGGSDVRSNASIAMPADPLEDGRGKAYLLSGHKWFFSAPMCD-----AHLMLAR 99 M +TE Q GSD+ A + A P DG Y L G K F + D HL+LAR Sbjct: 161 MNLTEPQAGSDL---ALVRTRAVPEGDG---TYKLFGTKIFITYGEHDMAENIVHLVLAR 214 Query: 100 TEN------GLSCFFVPRWLPD-----GTRNPILIQRLKDKLGNRSNSSSEVEFEE---A 145 T N G+S F VP++L + G RN + ++ KLG +++ ++ ++F + A Sbjct: 215 TPNAPEGVKGISLFIVPKFLVNADGSLGARNDVHCVSIEHKLGIKASPTAVLQFGDHGGA 274 Query: 146 MGIMVGEDGRGIPTIIEMANHTRLDCIIGSAALMRQALVQALHHARHRSAFGRRLA---- 201 +G +VGE+ RG+ + M N R I ++ +A +A+ +AR R R LA Sbjct: 275 IGTLVGEENRGLEYMFIMMNAARFAVGIQGLSVSERAYQKAVAYARDRIQ-SRDLAGSPG 333 Query: 202 -----EQPIMRAVLADLALESEAATLLMLRVAHAFDA---PDDPLQR----AWKRIVTPA 249 QP +R +L + + EAA L A DA DD R A+ + P Sbjct: 334 PVAIIHQPDVRRMLMSMRAQIEAARALSYVAAATSDAAHFSDDEATRKSNQAFYEYLVPI 393 Query: 250 AKFWICKRALEFTGECMEVWGGNGYVETGPMARLYREAPVNSIWEGSGNVMCLDVL-RAI 308 K WI + +++ T ++V GG G++E A+ YR+A + I+EG+ + D++ R Sbjct: 394 VKGWITEMSIDVTSTGVQVHGGMGFIEETGAAQYYRDARILPIYEGTTAIQANDLVGRKT 453 Query: 309 SRETEGFAQLLIA 321 +R+ A+ ++A Sbjct: 454 ARDGGAVAKGILA 466 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 85.5 bits (210), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 72/284 (25%), Positives = 128/284 (45%), Gaps = 21/284 (7%) Query: 25 SREHDGRDLPITQKRSILIGMGMTEKQGGSDVRSNASIAMPADPLEDGRGKAYLLSGHK- 83 + E + LP + + M+E GSDV S+ + AD +G Y+L+G K Sbjct: 109 NEEQKRKYLPRLISGDFIGALAMSEPNAGSDV---VSMKLRADK----KGDRYVLNGSKM 161 Query: 84 WFFSAPMCDAHLMLARTE-----NGLSCFFVPRWLPDGTRNPILIQRLKDKLGNRSNSSS 138 W + P D ++ A+T+ G++ F V + G + + Q+L DKLG R + + Sbjct: 162 WITNGPDADVLVVYAKTDLEAGARGMTAFLVEK----GYKGFSVAQKL-DKLGMRGSHTG 216 Query: 139 EVEFEEAM---GIMVGEDGRGIPTIIEMANHTRLDCIIGSAALMRQALVQALHHARHRSA 195 E+ F++ ++G GRG+ ++ + R G +M+ + + + R Sbjct: 217 ELVFQDCEVPEENVLGGVGRGVNVLMSGLDFERSVLSGGPLGIMQACMDVVVPYVHDRKQ 276 Query: 196 FGRRLAEQPIMRAVLADLALESEAATLLMLRVAHAFDAPDDPLQRAWKRIVTPAAKFWIC 255 FG+ + E +M+ LAD+ A + V A D D + R A + Sbjct: 277 FGQAIGEFQLMQGKLADMYSTMMACKAYVYAVGQACDRADSADKVRALRKDAAGAILYSA 336 Query: 256 KRALEFTGECMEVWGGNGYVETGPMARLYREAPVNSIWEGSGNV 299 ++A GE ++ GGNGY+ P+ RL+R+A + I G+ + Sbjct: 337 EKATWMAGEAIQSLGGNGYINEYPVGRLWRDAKLYEIGAGTSEI 380 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.134 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,454,350 Number of extensions: 61641 Number of successful extensions: 202 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 193 Number of HSP's successfully gapped: 8 Length of query: 454 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 366 Effective length of database: 1,203,190 Effective search space: 440367540 Effective search space used: 440367540 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)