BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0167.p (290 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310309|ref|YP_003774401.1| methyl-accepting chemotaxis tra... 463 e-132 gi|300312458|ref|YP_003776550.1| methyl-accepting chemotaxis sen... 223 1e-59 gi|300309601|ref|YP_003773693.1| methyl-accepting chemotaxis tra... 160 1e-40 gi|300313825|ref|YP_003777917.1| methyl-accepting chemotaxis tra... 147 1e-36 gi|300309839|ref|YP_003773931.1| metal-dependent phosphohydrolas... 57 2e-09 >gi|300310309|ref|YP_003774401.1| methyl-accepting chemotaxis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 625 Score = 463 bits (1191), Expect = e-132, Method: Compositional matrix adjust. Identities = 235/277 (84%), Positives = 257/277 (92%) Query: 1 MTSSSLRTRLIALCAGIVVLAMLAIVLTNFLTARSRTMSVLDGQMVQLAQSQASAIAEWS 60 M S+SLRTRLIALCAGIVVLAMLAIVLTNFLTARSRTMS LD QM QLAQSQA+AIAEWS Sbjct: 20 MKSTSLRTRLIALCAGIVVLAMLAIVLTNFLTARSRTMSTLDTQMTQLAQSQAAAIAEWS 79 Query: 61 DARKLVVSSIKQAADAAEPLPFVKAAETAGGFDLAYIGYADKHAVFSQQRNRAPDYDPTK 120 D++KLVV+SIK+AADAA+PLPFVKAA+TAGGFDLAY+GYADKHAVFSQQR+RAPDYDPTK Sbjct: 80 DSKKLVVTSIKEAADAADPLPFVKAAQTAGGFDLAYLGYADKHAVFSQQRSRAPDYDPTK 139 Query: 121 RPWYQLAASSKGPVLTAPYTSASTGKLLVTFAEAISKDGQVTAVAAADVLLDSVVADVVA 180 RPWYQ AA + PVLTAPYTSASTGKLLVTFA+++SKDGQV AVAAADVLLD+VVADVVA Sbjct: 140 RPWYQQAAKTSEPVLTAPYTSASTGKLLVTFAQSVSKDGQVAAVAAADVLLDTVVADVVA 199 Query: 181 IKPTPNSYAFLVDLGGKIIAHPNDKLRLKPLSDLDAAITPQSLAAAMAANSHQELALDGR 240 IKPTPNSYAFL DL GKIIAHPNDKLRLKPL+DLDAAITPQ+L AM HQ++ALDGR Sbjct: 200 IKPTPNSYAFLADLSGKIIAHPNDKLRLKPLADLDAAITPQTLDEAMRTGVHQDVALDGR 259 Query: 241 QQMLFAARVAGTDWLLVVAVDRADALASLTAMLASSG 277 +QMLF ARV GTDWLLVVA+DRADALASL+AML SSG Sbjct: 260 RQMLFVARVGGTDWLLVVALDRADALASLSAMLVSSG 296 >gi|300312458|ref|YP_003776550.1| methyl-accepting chemotaxis sensory transducer protein [Herbaspirillum seropedicae SmR1] Length = 684 Score = 223 bits (569), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 122/270 (45%), Positives = 165/270 (61%), Gaps = 5/270 (1%) Query: 4 SSLRTRLIALCAGIVVLAMLAIVLTNFLTARSRTMSVLDGQMVQLAQSQASAIAEWSDAR 63 SSLR RLI +C IV LAM+ + N T RS T+ + Q QL +S A+ I+EW ++ Sbjct: 3 SSLRARLIFICVLIVALAMIILSAANIFTVRSDTLEAISSQTQQLTESHAANISEWVRSK 62 Query: 64 KLVVSSIKQAADAAEPLPFVKAAETAGGFDLAYIGYADKHAVFSQQRNRAPDYDPTKRPW 123 ++V ++KQA ++ LP V AA+ AG FD AYIGY DK + YDPT RPW Sbjct: 63 RVVTGAMKQALKQSDVLPIVAAAQEAGSFDDAYIGYPDKRMLALHPM--PAGYDPTARPW 120 Query: 124 YQLAASSKGPVLTAPYTSASTGKLLVTFAEAISKDGQVTAVAAADVLLDSVVADVVAIKP 183 Y+ A + PVLTAPY A+TGKL+VTFAE + + AV +DV LD+VV V IKP Sbjct: 121 YKQAVEAGKPVLTAPYVDATTGKLVVTFAEPVGDKSNLQAVLGSDVQLDNVVRTVAGIKP 180 Query: 184 TPNSYAFLVDLGGKIIAHPNDKLRLKPLSDLDAAITPQSLAAAMAANSHQELALDGRQQM 243 TP+S+AF+V G II HPN +L LKP+S LD ++ QSLA S + G + + Sbjct: 181 TPSSFAFIVGKDGVIITHPNKELALKPVSSLDQTLSAQSLAGMKQGGSA---TIGGIRYL 237 Query: 244 LFAARVAGTDWLLVVAVDRADALASLTAML 273 LF +A TDW LVVA+D +A ++ A++ Sbjct: 238 LFTQPIANTDWSLVVALDYREATHAIGALM 267 >gi|300309601|ref|YP_003773693.1| methyl-accepting chemotaxis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 160 bits (406), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 95/266 (35%), Positives = 147/266 (55%), Gaps = 2/266 (0%) Query: 4 SSLRTRLIALCAGIVVLAMLAIVLTNFLTARSRTMSVLDGQMVQLAQSQASAIAEWSDAR 63 SSLR R+I +V+ ++L N+L AR + D + A+ I +W + Sbjct: 3 SSLRARVICALILLVISSVLISTAANYLIARKGLNAATDDDLATAVDQHAAVIGDWLAGK 62 Query: 64 KLVVSSIKQAADAAEPLPFVKAAETAGGFDLAYIGYADKHAVFSQQRNRAPDYDPTKRPW 123 +++S++ + ++P +K + AGGF +GY DK A F+ N YDPT RPW Sbjct: 63 SKMIASLEDISLTSDPDAMLKQIQVAGGFYDVGVGYPDKRAKFTDWPNIPATYDPTTRPW 122 Query: 124 YQLAASSKGPVLTAPYTSASTGKLLVTFAEAISKDGQVTAVAAADVLLDSVVADVVAIKP 183 Y+ + PV PY S S G LLV A + +DG + AV DV LDSVV ++ +I P Sbjct: 123 YKGVLQAGKPV-ALPYVSTS-GLLLVALARPVMRDGVLKAVVVGDVALDSVVENIKSIHP 180 Query: 184 TPNSYAFLVDLGGKIIAHPNDKLRLKPLSDLDAAITPQSLAAAMAANSHQELALDGRQQM 243 TP+S+ LV+ GG+IIAHP+ +LR K S+L + ++A A + +DGR+++ Sbjct: 181 TPSSFGMLVEQGGRIIAHPDAQLRFKQASELAPEFSDLNVANAGPGQPPVHIVVDGREKL 240 Query: 244 LFAARVAGTDWLLVVAVDRADALASL 269 + V GTDW +VVA+D+ +A A + Sbjct: 241 VRVQAVPGTDWNIVVAIDQQEASAGV 266 >gi|300313825|ref|YP_003777917.1| methyl-accepting chemotaxis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 598 Score = 147 bits (370), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 85/266 (31%), Positives = 142/266 (53%) Query: 4 SSLRTRLIALCAGIVVLAMLAIVLTNFLTARSRTMSVLDGQMVQLAQSQASAIAEWSDAR 63 +S++ R++ +++ ++ + +F + ++ +A S I EW AR Sbjct: 3 NSIKARILFYTITLLIASLAVVGTVSFFIIKLDNDRAIERNAESVASGFGSTINEWVAAR 62 Query: 64 KLVVSSIKQAADAAEPLPFVKAAETAGGFDLAYIGYADKHAVFSQQRNRAPDYDPTKRPW 123 + + A+P+ +K + +GGF + +G DK A S YDPT RPW Sbjct: 63 TAMTVAAADGVLNADPVFVIKTLQKSGGFYVTTLGKEDKTAFTSAAEGLPAGYDPTARPW 122 Query: 124 YQLAASSKGPVLTAPYTSASTGKLLVTFAEAISKDGQVTAVAAADVLLDSVVADVVAIKP 183 Y+ A ++ PVLT PYT +T K +V+F + ++GQ V AA + LD V V +I P Sbjct: 123 YKQAVAAGKPVLTKPYTDVATKKPMVSFTAPVMQNGQRAGVVAAAMFLDGVSDVVRSIHP 182 Query: 184 TPNSYAFLVDLGGKIIAHPNDKLRLKPLSDLDAAITPQSLAAAMAANSHQELALDGRQQM 243 TP S+AFLVD G ++AHP+ + KP + +A +TP+ L A A + E+ L+G ++ Sbjct: 183 TPASFAFLVDRDGLMLAHPDGQWVNKPAKEWNAELTPERLNALAADDKASEVMLEGAAKL 242 Query: 244 LFAARVAGTDWLLVVAVDRADALASL 269 + + GT+W L++A+D+AD A L Sbjct: 243 VKGVPIKGTEWTLMLAMDKADVTAGL 268 >gi|300309839|ref|YP_003773931.1| metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 975 Score = 56.6 bits (135), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 44/165 (26%), Positives = 73/165 (44%), Gaps = 24/165 (14%) Query: 72 QAADAAEPLPFVKAAETA------------GGFDLAYIGYADKHAVFSQQRNRAPDYDPT 119 +A AE F +A E+A G Y Y D + S + +DP Sbjct: 140 RALRGAEQRSFFRAPESARFLVQTITRNANGQEHGQYRFYDDDLRLLSLREQPEYHFDPR 199 Query: 120 KRPWYQLAASSKGPVLTAPYTSASTGKLLVTFAEAISKDGQVTAVAAADVLLDSVVADVV 179 RPWY A + P ++APY +T ++ ++ ++ + AV A D+ LD + + Sbjct: 200 TRPWYASANRALMPSISAPYVFFTTRQIGISLSQ---RAHASEAVVAIDMTLDDLATSLG 256 Query: 180 AIKPTPNSYAFLVDLGGKIIAHPN---------DKLRLKPLSDLD 215 ++ TP+S LVD GK+IA+ + D R + L +L+ Sbjct: 257 NLRLTPHSELALVDEAGKVIAYSDMNRVLEQDGDSFRFRSLDELN 301 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.128 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 699,296 Number of extensions: 23957 Number of successful extensions: 157 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 155 Number of HSP's successfully gapped: 5 Length of query: 290 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 206 Effective length of database: 1,222,130 Effective search space: 251758780 Effective search space used: 251758780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)