BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0163.p (337 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309449|ref|YP_003773541.1| TonB-dependent hemoglobin/trans... 82 8e-17 gi|300313664|ref|YP_003777756.1| tonB-dependent receptor for iro... 65 8e-12 gi|300310609|ref|YP_003774701.1| outer membrane Iron(III) dicitr... 63 3e-11 gi|300309529|ref|YP_003773621.1| TonB dependent ferrisiderophore... 61 1e-10 gi|300312681|ref|YP_003776773.1| TonB-dependent siderophore rece... 60 2e-10 >gi|300309449|ref|YP_003773541.1| TonB-dependent hemoglobin/transferrin/lactoferrin receptor protein [Herbaspirillum seropedicae SmR1] Length = 765 Score = 81.6 bits (200), Expect = 8e-17, Method: Compositional matrix adjust. Identities = 61/158 (38%), Positives = 80/158 (50%), Gaps = 19/158 (12%) Query: 38 VAQDAGAGLKEVVVTATGFEQEVVD-APASITVIGQEELKKRPFRDLTDALRDVEGVTI- 95 +AQ +G L+EVVVTA D A A+++VI EE+++ RD+ DALR GV + Sbjct: 48 LAQASGKKLQEVVVTANRSGSSDSDHAAATVSVISAEEIEQHNARDIKDALRYEPGVEVK 107 Query: 96 ---------------TGTASEKDIFIRGLPGAYTLILVDGKRQSTRDARTNGNSGFEQSY 140 TG + I IRGL G L+L DG S + + G S + Sbjct: 108 RSVYRMSGITGPGATTGRGGNEGISIRGLDGNRVLMLEDGI--SMPRSFSYGTSSVGRGA 165 Query: 141 IPPLEAIERIEVVRGPMSSLYGSDAMGGVINIITKKVA 178 E RIEV+RGP SSLYGSD + G +N ITK A Sbjct: 166 TTDTELYRRIEVLRGPASSLYGSDGLTGAVNFITKDPA 203 >gi|300313664|ref|YP_003777756.1| tonB-dependent receptor for iron transport protein [Herbaspirillum seropedicae SmR1] Length = 798 Score = 65.1 bits (157), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 51/151 (33%), Positives = 77/151 (50%), Gaps = 31/151 (20%) Query: 44 AGLKEVVVTAT--------------GFEQEVVDAPASITVIGQEELKKRPFRDLTDALRD 89 +GLKEVVVTA F Q ++D P SITV+ +E L ++ ++L D L++ Sbjct: 59 SGLKEVVVTAPREAPEYKAQAVSSPKFTQPLLDLPQSITVVPKEVLTQQNAQNLQDILKN 118 Query: 90 VEGVTIT---GTASEKDIF-IRGLPGAYTLILVDGKRQSTRDARTNGNSGFEQSYIPPLE 145 V G+T T G D+F IRG +L VDG R + +R N F Sbjct: 119 VPGITFTSGEGNLGWGDMFTIRGFSSEQSLT-VDGVRDAGMSSR---NDTFN-------- 166 Query: 146 AIERIEVVRGPMSSLYGSDAMGGVINIITKK 176 +E++EV +G S G A+GG +N+++K+ Sbjct: 167 -LEQVEVFKGTGSIESGVSAIGGSVNLVSKE 196 >gi|300310609|ref|YP_003774701.1| outer membrane Iron(III) dicitrate transport protein [Herbaspirillum seropedicae SmR1] Length = 703 Score = 63.2 bits (152), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 75/275 (27%), Positives = 115/275 (41%), Gaps = 28/275 (10%) Query: 6 HHLPALGA--LSVTRMAQAVAAALALYVSGCGL-AVAQDAGAGLKEVVVTA----TGFEQ 58 H P+L L +T A A A LA V+G A D GL+ + V+ TG E Sbjct: 4 HQAPSLFVRDLRITTTALASLAILAFPVAGLAQQASPSDGQPGLEPIQVSGDWLGTGLES 63 Query: 59 EVVDAPASITVIGQEELKKRPFRDLTDALRDVEGVTIT------GTASEKDIFIRGLPGA 112 V P + TV+ ++E++ + DA+R + GV + G+A +I +RGL G Sbjct: 64 SVKTYPGARTVVKKQEIESTGAVSIGDAMRRIPGVQSSDNSGTAGSAIALNIGVRGLAGR 123 Query: 113 YT---LILVDGKRQSTRDARTNGNSGFEQSYIPPL--EAIERIEVVRGPMSSLYGSDAMG 167 Y+ IL+DG + G Q PL IE I+VVRG + +G +G Sbjct: 124 YSPRSTILLDGIPMAV------APYGQPQLSFAPLSLNNIESIDVVRGGGAVRFGPQNVG 177 Query: 168 GVINIITKKVARQWGGSLSADTTLQQHGDSGDAYQGQFYVNGPIASEVLGLQLWGKVYKR 227 G+IN T+ + G S A +G G Q + G A G+ +Y Sbjct: 178 GIINFKTRSIPTTPGISGDAAVRYNSYGQGGGNTQYTAFAGGQ-AENGFGMAF---LYSG 233 Query: 228 QEDRILNGNTKALDTDFTARLALTPNKNHDILAEI 262 QE + DF ++ + ++ A+I Sbjct: 234 QEGSGWRDKSDEHLNDFALKMRYEISPTSELYAKI 268 >gi|300309529|ref|YP_003773621.1| TonB dependent ferrisiderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 704 Score = 61.2 bits (147), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 41/130 (31%), Positives = 64/130 (49%), Gaps = 21/130 (16%) Query: 51 VTATGFEQEVVDAPASITVIGQEELKKRPFRDLTDALRDVEGVTI-TGTASEKDIFIRGL 109 V+ T E + D P +I V+ + ++ + L D L++V G+ + TG +FIRG Sbjct: 55 VSGTKSEAPLRDVPQTINVVPAQVIRDQNASSLQDVLKNVPGLGLSTGDGQRDQVFIRG- 113 Query: 110 PGAYTLI---LVDGKRQSTRDARTNGNSGFEQSYIPPLEAIERIEVVRGPMSSLYGSDAM 166 +T I VDG R + Y L +E +EV++GP + LYG + Sbjct: 114 ---FTAIGDQFVDGFRD-------------DALYFRDLSNVESVEVIKGPAAVLYGRGSS 157 Query: 167 GGVINIITKK 176 GG+IN +TKK Sbjct: 158 GGLINRVTKK 167 >gi|300312681|ref|YP_003776773.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 738 Score = 60.5 bits (145), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 39/126 (30%), Positives = 65/126 (51%), Gaps = 17/126 (13%) Query: 54 TGFEQEVVDAPASITVIGQEELKKRPFRDLTDALRDVEGVTITGTASEK---DIFIRGLP 110 T ++ D P S+TV+ + +++ + L+DALR+V G+T+ G + +I + G Sbjct: 62 TKLPADLRDVPQSVTVVNKAQMQSQGASSLSDALRNVSGITLGGAEGGQIGNNINLNGF- 120 Query: 111 GAYTLILVDGKRQSTRDARTNGNSGFEQSYIPPLEAIERIEVVRGPMSSLYGSDAMGGVI 170 A T I +DG R + Y AI+ +EV+ GP S L+G + GG+I Sbjct: 121 SARTDIYLDGFRDRGQ-------------YYRDTFAIDEVEVLMGPSSMLFGRGSTGGII 167 Query: 171 NIITKK 176 N ++KK Sbjct: 168 NQVSKK 173 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.130 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 960,397 Number of extensions: 39977 Number of successful extensions: 175 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 170 Number of HSP's successfully gapped: 10 Length of query: 337 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 251 Effective length of database: 1,212,660 Effective search space: 304377660 Effective search space used: 304377660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)