BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0159.p (223 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313666|ref|YP_003777758.1| two component sensor histidine ... 134 8e-33 gi|300310848|ref|YP_003774940.1| two component sensor histidine ... 127 5e-31 gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbas... 125 4e-30 gi|300310697|ref|YP_003774789.1| two component hybrid sensor his... 86 2e-18 gi|300311272|ref|YP_003775364.1| two component sensor histidine ... 83 2e-17 >gi|300313666|ref|YP_003777758.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 479 Score = 134 bits (336), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 91/224 (40%), Positives = 129/224 (57%), Gaps = 27/224 (12%) Query: 1 MLAAISHDLRTPITSLRLRAELVDDAALRTAMIRTLDEMRVMVEETVRFARDDAHAEATR 60 LAA+SHDLR+PIT ++LR E++ D + + LDEM ++ T+ +D E + Sbjct: 280 FLAAVSHDLRSPITRMKLRTEMLPDERGKEKFRKDLDEMDALISATLNVVQDIDVQERPQ 339 Query: 61 EIDLAELLQELAQEHASQGRDVQITVQKNAQQKVQNNVLHETLAQQDAPLRYRCRPVHLK 120 IDL LL+ L + A G +V TL+ APL + P L+ Sbjct: 340 LIDLDSLLESLRTDQAEVGGEV-------------------TLSGHAAPL--QGYPRSLR 378 Query: 121 RALSNLVDNAVRHGQRARLSLIVEHDGSLSILVDDDGPGIAEEHLQEVFKPFVRLDPARS 180 R L NLV+NAVR+GQRAR+ L + D + I VDDDGPGI E L+ V +PF RL+ +R+ Sbjct: 379 RCLQNLVENAVRYGQRARVQLQDDAD-QVCIRVDDDGPGIPEALLERVQEPFYRLERSRN 437 Query: 181 GGN--FGLGLAIARSCIEAHGGLLELRNRRQPGDQGLQACISLP 222 G FGLGL+IA + ++AH G+++L NR PG GL+ I +P Sbjct: 438 AGTGGFGLGLSIAATVVQAHRGVMKLVNR--PGG-GLRVEILIP 478 >gi|300310848|ref|YP_003774940.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 465 Score = 127 bits (320), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 85/224 (37%), Positives = 122/224 (54%), Gaps = 26/224 (11%) Query: 1 MLAAISHDLRTPITSLRLRAELVDDAALRTAMIRTLDEMRVMVEETVRFARDDAHAEATR 60 MLAAI+HDL+TP+T +RLR E V D L+ +I L M MV E + AR +E + Sbjct: 258 MLAAITHDLQTPLTRMRLRLEKVSDPGLQQKLIDDLGVMHGMVREGLDLARSMDSSEKIQ 317 Query: 61 EIDLAELLQELAQEHASQGRDVQITVQKNAQQKVQNNVLHETLAQQDAPLRYRCRPVHLK 120 +D+ LL + + A G+DV + Q + +AQ P L+ Sbjct: 318 ALDIDSLLDSVCADAADAGQDVAL----------QGSTRAFVMAQ----------PGALR 357 Query: 121 RALSNLVDNAVRHGQRARLSLIVEHDGSLSILVDDDGPGIAEEHLQEVFKPFVRLDPARS 180 R L+NL+DNA ++G+ AR+S+ +E ++I + D GPGI E L+ VF+PF RL+ +RS Sbjct: 358 RCLTNLIDNACKYGKSARVSIALEQQ-KIAIRIRDQGPGIPESELEAVFEPFYRLEASRS 416 Query: 181 --GGNFGLGLAIARSCIEAHGGLLELRNRRQPGDQGLQACISLP 222 G GLGL IA + E H G L LRN + GL+ + LP Sbjct: 417 RDTGGTGLGLTIALNIAENHHGQLRLRNL---AEGGLEVLLELP 457 >gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 478 Score = 125 bits (313), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 84/229 (36%), Positives = 125/229 (54%), Gaps = 31/229 (13%) Query: 1 MLAAISHDLRTPITSLRLRAELVDDAALRTAMIRTLDEMRVMVEETVRFARDDAHAEATR 60 + +ISHDLRTPIT L+LR EL+DD A+RT LDE+ +MV+ ++ +D E Sbjct: 274 LFVSISHDLRTPITRLKLRTELMDDDAMRTDFHDDLDELDMMVKGALQSVKDSDIHENRT 333 Query: 61 EIDLAELLQELAQEHASQGRDVQITVQKNAQQKVQNNVLHETLAQQDAPLRYRCRPVHLK 120 E+ L L+ + ++ G ++ T NV+ +P+ LK Sbjct: 334 EVRLDALVLRMIRDARLAGHEIAFTPSG-------INVI--------------AKPLALK 372 Query: 121 RALSNLVDNAVRHGQRARLSLIVEHDGSLSILVD----DDGPGIAEEHLQEVFKPFVRLD 176 RA+ NL DNA+ +GQ+ +S I G+ LV+ D GPG+ E+ + +F+P+VRL+ Sbjct: 373 RAIGNLFDNALYYGQKVEIS-IRPSPGAEGDLVEIEIRDHGPGVPEDAMGTLFQPYVRLE 431 Query: 177 PARS--GGNFGLGLAIARSCIEAHGGLLELRNRRQPGDQGLQACISLPA 223 R+ G GLGL I+R+ ++AHGG L LRN + GL A I LPA Sbjct: 432 HGRAQNSGGMGLGLGISRNIVQAHGGELLLRNHPE---GGLMATIILPA 477 >gi|300310697|ref|YP_003774789.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 916 Score = 86.3 bits (212), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 79/238 (33%), Positives = 113/238 (47%), Gaps = 33/238 (13%) Query: 1 MLAAISHDLRTPITSLRLRAELVDDAALRTA-----MIRTLDEMRVMVEETVRFARDDA- 54 L ISHDLRTP+TS+ A+++ + A ++R+ M MV + + +AR D Sbjct: 454 FLTRISHDLRTPLTSILGFADMIQASGGPHAGHGRIIMRSARHMLTMVNDLIDYARGDPL 513 Query: 55 HAEATREIDLAELLQELAQEHASQGRDVQITVQKNAQQKVQNNVLHETL-AQQDAPLRYR 113 + L LLQE+AQE Q+ Q+ Q+N H TL D P Sbjct: 514 DTPQPTPLYLHALLQEIAQEG-------QVLAQR------QHN--HFTLDISPDLPPVLA 558 Query: 114 CRPVHLKRALSNLVDNAVRHGQRARLSLIVEH--------DGSLSILVDDDGPGIAEEHL 165 L R L NL+DNA ++ + R+ L V+ G+L I V D G GIA + Sbjct: 559 LDARRLHRILGNLLDNAAKYTREGRIGLHVQWQADHADAGKGALQIEVSDTGCGIAPQFQ 618 Query: 166 QEVFKPFVRLDPARSGGNFGLGLAIARSCIEAHGGLLELRNRRQPGDQGLQACISLPA 223 +F+PF R D R G+GLAI R ++ GG + L + QPG G + + LPA Sbjct: 619 SRIFEPFERADADRRQPGIGMGLAIVRLWVQRMGGSIVLES--QPG-MGTRMHLQLPA 673 >gi|300311272|ref|YP_003775364.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 82.8 bits (203), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 68/221 (30%), Positives = 105/221 (47%), Gaps = 28/221 (12%) Query: 3 AAISHDLRTPITSLRLRAELVDDAAL------RTAMIRTLDEMRVMVEETVRFARDDAH- 55 AAI+H+LRTP+T+ + R + + D + ++R LD++ ++ + + A Sbjct: 180 AAIAHELRTPLTAAKARLQGIMDGIFDRSPQQLSMIMRQLDQLNRLISDLYLLSLVSAGQ 239 Query: 56 -AEATREIDLAELLQELAQEHASQGRDVQITVQKNAQQKVQNNVLHETLAQQDAPLRYRC 114 A + L LL+E A R + ++ + + N + L Q Sbjct: 240 LALVRSQFQLRPLLEERLAWAAPSLRLLPMSTALYCCEHLPLNADRDRLGQ--------- 290 Query: 115 RPVHLKRALSNLVDNAVRHGQRARLSLIVE--HDGSLSILVDDDGPGIAEEHLQEVFKPF 172 A+S L+DNA+R+ +L I HDG + I V+DDGPG A EHL+ F Sbjct: 291 -------AISILIDNALRYAASGKLLTISAGLHDGQIHIDVEDDGPGFAPEHLELACDRF 343 Query: 173 VRLDPARS--GGNFGLGLAIARSCIEAHGGLLELRNRRQPG 211 R + +RS G GLGLA+A + EAHGG L + NR G Sbjct: 344 WRAEHSRSRHAGGSGLGLAVAVAICEAHGGKLSVHNRPAGG 384 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 609,690 Number of extensions: 23195 Number of successful extensions: 228 Number of sequences better than 1.0e-08: 32 Number of HSP's gapped: 176 Number of HSP's successfully gapped: 32 Length of query: 223 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 141 Effective length of database: 1,231,600 Effective search space: 173655600 Effective search space used: 173655600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)