BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0030.0158.p
         (223 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311017|ref|YP_003775109.1| glutathione S-transferase famil...   412   e-117
gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herb...    61   6e-11

>gi|300311017|ref|YP_003775109.1| glutathione S-transferase family
           protein [Herbaspirillum seropedicae SmR1]
          Length = 223

 Score =  412 bits (1060), Expect = e-117,   Method: Compositional matrix adjust.
 Identities = 198/223 (88%), Positives = 213/223 (95%)

Query: 1   MITVHHLNNSRSQRVLWLLEELGLDYEIKFYQRDPKTMLAPPDLLKVHPLGKSPVITDGD 60
           MITVHHLNNSRSQRVLWLLEELGLDYEIKFYQRDPKTMLAPP+LLKVHPLGKSPVITDG+
Sbjct: 1   MITVHHLNNSRSQRVLWLLEELGLDYEIKFYQRDPKTMLAPPELLKVHPLGKSPVITDGE 60

Query: 61  LTVAESGNIVEYLLERYGADSGLLPAPGTPERLRYRYWLHFAEGSAMPWLLLGLIFSKMP 120
           LTVAESGNI+EYLLERYGA SGLLPA GTPERLRYRYWLHFAEGSAMPWL++ LIF+KMP
Sbjct: 61  LTVAESGNIIEYLLERYGAHSGLLPASGTPERLRYRYWLHFAEGSAMPWLVMQLIFAKMP 120

Query: 121 KAVPALIRPIARMLSEGMRSRLVAPQIERQMRYMEDELGKDGWFAGKSITGADIQMSFPV 180
           KAVP LIRPIARML+EGMRSR+VAPQ ERQ+R+MEDELG+ GWFAG   +GADIQMSFPV
Sbjct: 121 KAVPTLIRPIARMLAEGMRSRVVAPQQERQLRFMEDELGQQGWFAGAQFSGADIQMSFPV 180

Query: 181 EGMRARGGLDGRHPNLLKFLEAIHARPAYQRALEKGGPYELLR 223
           E +RARGGLDGR+PNLLK+LEAIHARPAYQRALEKGGPYELLR
Sbjct: 181 EALRARGGLDGRYPNLLKYLEAIHARPAYQRALEKGGPYELLR 223


>gi|300312317|ref|YP_003776409.1| glutathione S-transferase
           [Herbaspirillum seropedicae SmR1]
          Length = 230

 Score = 61.2 bits (147), Expect = 6e-11,   Method: Compositional matrix adjust.
 Identities = 65/232 (28%), Positives = 90/232 (38%), Gaps = 49/232 (21%)

Query: 1   MITVHHLNNSRSQRVLWLLEELGLDYEIKFYQRDPKTMLAPPDLLKVHPLGKSPVITDGD 60
           MI V+        +V  +LEE GL Y              P + LK+ P  K P I D D
Sbjct: 1   MIDVYSWATPNGHKVHIMLEECGLPYTAHAIDIGAGDQFTP-EFLKISPNNKIPAIVDPD 59

Query: 61  ------LTVAESGNIVEYLLERYGADSGLLPAPGTPERLRYRYWLHFAEGSAMPWLLLGL 114
                 +++ ESG I+ YL       +G      T E+     W+ F  G   P      
Sbjct: 60  GPDGEPISLFESGAILIYL----AGKTGRFLGETTREKYEVLQWVMFQMGGLGP------ 109

Query: 115 IFSKMPKAVPALIRPIARMLSEGMRSRLVAP-QIE-----------RQMRYMEDELGKDG 162
                             ML +    RL AP QIE           R  + M+ +LG+  
Sbjct: 110 ------------------MLGQAHHFRLYAPEQIEYAVNRYTNEARRLYQVMDKQLGQHA 151

Query: 163 WFAGKSITGADIQMSFPVEGMRARGGLD-GRHPNLLKFLEAIHARPAYQRAL 213
           + AG+  T ADI   FP        G+D    PN+ ++ +AI ARPA QR +
Sbjct: 152 YLAGEHYTIADI-ACFPWTRSHKNQGIDLDDFPNVKRWYDAISARPAVQRGV 202


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.321    0.140    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 812,045
Number of extensions: 36649
Number of successful extensions: 110
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 110
Number of HSP's successfully gapped: 2
Length of query: 223
Length of database: 1,619,870
Length adjustment: 82
Effective length of query: 141
Effective length of database: 1,231,600
Effective search space: 173655600
Effective search space used: 173655600
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 129 (54.3 bits)