BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0153.p (775 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311015|ref|YP_003775107.1| anaerobic (selenocysteine-conta... 1593 0.0 gi|300310774|ref|YP_003774866.1| anaerobic formate dehydrogenase... 721 0.0 gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydroge... 139 1e-33 gi|300312208|ref|YP_003776300.1| assimilatory nitrate reductase ... 97 7e-21 gi|300313158|ref|YP_003777250.1| anaerobic dehydrogenase family ... 60 9e-10 >gi|300311015|ref|YP_003775107.1| anaerobic (selenocysteine-containing) formate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 775 Score = 1593 bits (4124), Expect = 0.0, Method: Compositional matrix adjust. Identities = 758/775 (97%), Positives = 771/775 (99%) Query: 1 MPSKPRIEQYNQPAGGWGALKYVALNLVKEHVADGKGLRTLLSQNQPDGFDCPGCAWPDR 60 MPSKPRIEQYNQPAGGWGALKYVALNLVKEHVADG+GLRTLLSQNQPDGFDCPGCAWPDR Sbjct: 1 MPSKPRIEQYNQPAGGWGALKYVALNLVKEHVADGRGLRTLLSQNQPDGFDCPGCAWPDR 60 Query: 61 EHTSTFEFCENGVKAVAAEATKKRVTPEFFDKHTVTELMQQSDYELEEHGRLTHPMVYDA 120 EHTSTFEFCENGVKAVAAEATKKRVTPEFF +HTVTELMQQSD+ELEEHGRLTHPMVYDA Sbjct: 61 EHTSTFEFCENGVKAVAAEATKKRVTPEFFAQHTVTELMQQSDFELEEHGRLTHPMVYDA 120 Query: 121 ATDKYKKIDWSAAFELMAKHLNALPDPDMADFYVSGRASNEAAFLFQLFVRQYGTNNFPD 180 ATDKYKKI+W+ AF LMAKHLNALPDPDMADFYVSGRASNEAAFLFQLFVRQYGTNNFPD Sbjct: 121 ATDKYKKIEWADAFALMAKHLNALPDPDMADFYVSGRASNEAAFLFQLFVRQYGTNNFPD 180 Query: 181 CSNMCHEPTSVGLPGTVGIGKGTVLLEDFDHCDTLLLFGQNPATNHPRMMGELRHASKRG 240 CSNMCHEPTSVGLPGTVGIGKGTVLLEDFDHCDTLLLFGQNPATNHPRMMGELRHASKRG Sbjct: 181 CSNMCHEPTSVGLPGTVGIGKGTVLLEDFDHCDTLLLFGQNPATNHPRMMGELRHASKRG 240 Query: 241 ATIVAINPLKERGLERFADPQSKVEMLTMGSTRISSMFIHPTLGGDLALIKGVIKRTIEL 300 ATIVAINPLKERGLERFADPQSKVEMLTMGSTRISSMFIHPTLGGDLALIKGVIKRTIEL Sbjct: 241 ATIVAINPLKERGLERFADPQSKVEMLTMGSTRISSMFIHPTLGGDLALIKGVIKRTIEL 300 Query: 301 DDAAKAAGAERVIDVKFIEEHTAGFEEFAAQARAESWDDIIAESGVSREDIEKLAQVYVK 360 DDAA+AAGAERVIDVKFIEEHTAGFEEFAAQARAESWDDI+AESGVSREDIEKLAQVYVK Sbjct: 301 DDAARAAGAERVIDVKFIEEHTAGFEEFAAQARAESWDDIVAESGVSREDIEKLAQVYVK 360 Query: 361 GKAVIATWGMGLTQHKYAVATIQLLSNMMMLRGNIGRQGAGLCPVRGHSNVQGDRTVGID 420 GKAVIATWGMGLTQHK+AVATIQLLSNMMMLRGNIGRQGAGLCPVRGHSNVQGDRTVGID Sbjct: 361 GKAVIATWGMGLTQHKHAVATIQLLSNMMMLRGNIGRQGAGLCPVRGHSNVQGDRTVGID 420 Query: 421 EKPTPAFLDRLEQVFGFKAPRHHGNDTVGSVMAMLEGRTKVFIGLGGNFAMATPDTPRTF 480 EKPTPAFLDRLEQVFGFKAPRHHGNDTVGSVMAMLEGRTKVFIGLGGNFAMATPDTPRTF Sbjct: 421 EKPTPAFLDRLEQVFGFKAPRHHGNDTVGSVMAMLEGRTKVFIGLGGNFAMATPDTPRTF 480 Query: 481 EAMRSCNLTVHITTKLNRSHLVHGKDALILPTMGRTEIDVQKSGPQGVTVEDSMSMVHVS 540 EAMRSCNLTVHITTKLNRSHLVHGKDALILPTMGRTEID+QKSGPQGVTVEDSMSMVHVS Sbjct: 481 EAMRSCNLTVHITTKLNRSHLVHGKDALILPTMGRTEIDMQKSGPQGVTVEDSMSMVHVS 540 Query: 541 YGINKPASEHLMSETAIVAHLAEATMKSRNNGPKIDWLWYAEDYSRIRDAIEKVYDAFKG 600 YGINKPASEHLMSETAIVAHLAEATMKSRNNGPKIDWLWYAEDYSRIRDAIE+VYDAFKG Sbjct: 541 YGINKPASEHLMSETAIVAHLAEATMKSRNNGPKIDWLWYAEDYSRIRDAIEQVYDAFKG 600 Query: 601 YNERIANPGGFHLGVASRDRVWKTGSGKANFIVHEIPKDTPIHRARAIHGEKLLTLMTTR 660 YNERIANPGGFHLGVASRDRVWKT SGKANFIVH+IPKDTPIHRARAIHGEKLLTLMTTR Sbjct: 601 YNERIANPGGFHLGVASRDRVWKTASGKANFIVHDIPKDTPIHRARAIHGEKLLTLMTTR 660 Query: 661 SHDQYNTTIYGMDDRYRGVFGQRRVVFINPADLQMLGLKDGEWVDITSVWDDGVERRADG 720 SHDQYNTTIYGMDDRYRGVFGQRRVVFINPADLQMLGLKDGEWVDITSVWDDGVERRA+G Sbjct: 661 SHDQYNTTIYGMDDRYRGVFGQRRVVFINPADLQMLGLKDGEWVDITSVWDDGVERRAEG 720 Query: 721 FLLVEYDIPRGCIGSYYPETNPLVPLESFADKARTPTSKSIPVLLSRSAVQRSAA 775 FLLVEY+IPRGCIGSYYPETNPLVPLESFADKARTPTSKSIPVLLSRSAVQRSAA Sbjct: 721 FLLVEYEIPRGCIGSYYPETNPLVPLESFADKARTPTSKSIPVLLSRSAVQRSAA 775 >gi|300310774|ref|YP_003774866.1| anaerobic formate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 790 Score = 721 bits (1860), Expect = 0.0, Method: Compositional matrix adjust. Identities = 363/773 (46%), Positives = 487/773 (63%), Gaps = 23/773 (2%) Query: 1 MPSKPRIEQYNQPAGGWGALKYVALNLVKEHVADGKGLRTLLSQNQPDGFDCPGCAWPDR 60 +P ++YN AGGWG++K + L +EHV G R L+ QN+P+GF C C+W Sbjct: 23 IPEDATFDEYNNAAGGWGSVKALGSILQQEHVI-ASGTRILMHQNKPEGFACVSCSWAKP 81 Query: 61 EHTSTFEFCENGVKAVAAEATKKRVTPEFFDKHTVTELMQQSDYELEEHGRLTHPMVYDA 120 FEFCENG KA A E T+KR T FF +HTVTE+ +D ELE GR+T PM YDA Sbjct: 82 ADPHLFEFCENGAKATAWEITRKRATAAFFAQHTVTEMEAWNDLELESSGRVTEPMKYDA 141 Query: 121 ATDKYKKIDWSAAFELMAKHLNALPDPDMADFYVSGRASNEAAFLFQLFVRQYGTNNFPD 180 A+D+Y + WS AF+ +A L A+ DP FY SGRAS E ++L+QL R YGTNN PD Sbjct: 142 ASDRYLPVSWSEAFDDIAAQLRAM-DPKKVVFYASGRASLETSYLYQLMARMYGTNNLPD 200 Query: 181 CSNMCHEPTSVGLPGTVGIGKGTVLLEDFDHCDTLLLFGQNPATNHPRMMGELRHASKRG 240 SNMCHE TSV LP T+G+ GTV L+DF D +L FG N TN PRM+ L KRG Sbjct: 201 SSNMCHESTSVALPQTIGVPVGTVTLDDFGQTDCILFFGHNTGTNAPRMLHPLEEVRKRG 260 Query: 241 ATIVAINPLKERGLERFADPQSKVEMLTMGSTRISSMFIHPTLGGDLALIKGVIKRTIEL 300 ++ NPL+ERGLERF +PQS EM+T T IS+ ++ +GGD A G+ KR +E Sbjct: 261 VPVITFNPLRERGLERFVNPQSPKEMVTPAHTDISTQYLQIKIGGDSAAAIGMAKRILER 320 Query: 301 DDAAKAAGAERVIDVKFIEEHTAGFEEFAAQARAESWDDIIAESGVSREDIEKLAQVYVK 360 DD A+ G R++D+ FIEEHT+GFE+FA RA WD++ +GVSR ++E A Y Sbjct: 321 DDKARQEGLPRLLDLAFIEEHTSGFEQFADAVRAAPWDELERHAGVSRAELELAADTYAG 380 Query: 361 GKAVIATWGMGLTQHKYAVATIQLLSNMMMLRGNIGRQGAGLCPVRGHSNVQGDRTVGID 420 + +GMG+TQH+ AV TI +L+N++++RGNIG+ GAG+CP+RGHSNVQG RTVGI Sbjct: 381 AGRAMLMYGMGVTQHREAVRTIHMLTNLLLMRGNIGKPGAGICPIRGHSNVQGQRTVGIT 440 Query: 421 EKPTPAFLDRLEQVFGFKAPRHHGNDTVGSVMAMLEGRTKVFIGLGGNFAMATPDTPRTF 480 EKP D+L ++ F+ P G +TV + + +G F LGGNF A PD Sbjct: 441 EKPELVPNDKLRALYHFEPPMEKGLNTVEACEKIRDGELSAFFMLGGNFVRAIPDHGVME 500 Query: 481 EAMRSCNLTVHITTKLNRSHLVHGKDALILPTMGRTEIDVQKSGPQGVTVEDSMSMVHVS 540 A R LTV + T NRS ++HG+ + +LP +GR EID Q+ G Q V+VEDS +H S Sbjct: 501 AAWRKLPLTVQVVTHFNRSCVIHGQTSYVLPCLGRIEIDRQRGGEQAVSVEDSTGCMHGS 560 Query: 541 YGINKPASEHLMSETAIVAHLAEATMKSRNNGPKIDWLWYAEDYSRIRDAIEKVY-DAFK 599 G +PAS HL+SE AIVA LA+ + P++DW + DY+RIRDAIE Y D F+ Sbjct: 561 RGRAEPASPHLLSEPAIVAELAKRLLPF---NPQVDWDGWVGDYARIRDAIEATYPDIFR 617 Query: 600 GYNERIANPGGFHLGVASRDRVWKTGSGKANFIV-------HEIPKDTPIHRARAIHGEK 652 +N R+ NPGGF +A+R+R WKT +G+ANF+ ++P D P + Sbjct: 618 DFNARMWNPGGFARPLAARERQWKTPNGRANFMTPDGFDPDPDMPGDAP----------E 667 Query: 653 LLTLMTTRSHDQYNTTIYGMDDRYRGVFGQRRVVFINPADLQMLGLKDGEWVDITSVWDD 712 +L LMTTR Q+NTT+Y +DDR+RGV+G RRV+ +N DLQ GL G++V +V D Sbjct: 668 VLRLMTTRGDSQFNTTVYSLDDRFRGVWGTRRVLLMNLRDLQQHGLAAGDFVCAETVSAD 727 Query: 713 GVERRADGFLLVEYDIPRGCIGSYYPETNPLVPLESFADKARTPTSKSIPVLL 765 GVERR + +DIP GC+ Y+PE N L+PL A ++ P +KS+P+ L Sbjct: 728 GVERRVPHLRVEPFDIPVGCVMGYFPELNRLIPLFHHARGSKVPAAKSVPIRL 780 >gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydrogenase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 957 Score = 139 bits (350), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 101/397 (25%), Positives = 167/397 (42%), Gaps = 42/397 (10%) Query: 111 RLTHPMVYDAATDKYKKIDWSAAFELMA---KHLNALPDPDMADFYVSGRASNEAAFLFQ 167 R+ +PM+ + D ++++ W A A + + A D S R +NE +L Q Sbjct: 298 RILNPMIRKSINDPWREVSWEEALSYAASEFRRIQAKHGKDSIGGITSSRCTNEETYLVQ 357 Query: 168 LFVRQ-YGTNNFPDCSNMCHEPTSVGLPGTVGIGKGTVLLEDFDHCDTLLLFGQNPATNH 226 VR +G NN C+ +CH PT GL T+G GT E D +L+ G NPA H Sbjct: 358 KLVRAAFGNNNVDTCARVCHSPTGYGLKQTLGESAGTQTFESVMKSDVILIMGANPAAGH 417 Query: 227 PRMMGELRHASKRGATIVAINPLKERGLERFADPQSKVEMLTMGSTRISSMFIHPTLGGD 286 P +++ ++GA ++ I+P R + M P + D Sbjct: 418 PVFASQIKRRVRQGAKLIVIDP------------------------RTTEMVKSPHIQAD 453 Query: 287 LALIKGVIKRTIELDDAAKAAGAERVIDVKFIEEHT-----AGFEEFAAQARAESWDDII 341 L T + A +E + D +I+E ++EF + S + + Sbjct: 454 YHLKLRPGTNTAIITALAHVILSEGLQDEAYIQERCDLQSYQDWKEFVLR-EDNSPEAMA 512 Query: 342 AESGVSREDIEKLAQVYVKGKAVIATWGMGLTQHKYAVATIQLLSNMMMLRGNIGRQGAG 401 A +GV E I A++Y G +G+G+T+H T+ ++N+ M GN+GR+G G Sbjct: 513 AITGVPAEIIRGAARLYATGGNAAIYYGLGVTEHAQGSTTVMGIANLAMCTGNVGREGVG 572 Query: 402 LCPVRGHSNVQGDRTVGIDEKPTPAF--------LDRLEQVFGFKAPRHHGNDTVGSVMA 453 + P+RG +NVQG +G P + ++ EQ +G G A Sbjct: 573 VNPLRGQNNVQGSCDMGSFPHELPGYRHISDSTVRNQFEQAWGVTLNPEPGLRIPNMFDA 632 Query: 454 MLEGRTKVFIGLGGNFAMATPDTPRTFEAMRSCNLTV 490 L+G K G + + P+T A+++ V Sbjct: 633 ALDGSFKGLYCEGEDIVQSDPNTQHVAAALQAMECIV 669 >gi|300312208|ref|YP_003776300.1| assimilatory nitrate reductase large subunit [Herbaspirillum seropedicae SmR1] Length = 922 Score = 96.7 bits (239), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 105/431 (24%), Positives = 173/431 (40%), Gaps = 56/431 (12%) Query: 110 GRLTHPMVYDAATDKYKKIDWSAAFELMAKHLNAL---PDPDMADFYVSGRASNEAAFLF 166 GRL HP + DA + +++ W A + +A +A+ PD YVSG+ E ++ Sbjct: 68 GRLLHPQLRDA-QGQLQQLAWDQALDHVASKFSAIIAEHGPDAVALYVSGQLLTEDYYIA 126 Query: 167 QLFVRQY-GTNNFPDCSNMCHEPTSVGLPGTVGIGKGTVLLEDFDHCDTLLLFGQNPATN 225 ++ Y G+ N S +C G G V ED + D ++L G N A Sbjct: 127 NKLMKGYIGSANIDTNSRLCMSSAVAGHKRAFGEDLVPVCYEDLELADLVVLVGSNAAWC 186 Query: 226 HPRMMGEL-RHASKRGAT-IVAINPLKERGLERFADPQSKVEMLTMGSTRISSMFIHPTL 283 HP + + R R A +V I+P + E ++ + + Sbjct: 187 HPILFQRIARLKETRPALRLVVIDPRRTATCE------------------LADLHLPIKP 228 Query: 284 GGDLALIKGVIKRTIELDDAAKAAGAERVI-DVKFIEEHTAGFEEFAAQARAESWD--DI 340 G D+ L G++ A R + D FIE HT GF+E A A A+ D + Sbjct: 229 GTDVWLFNGLMSYL-----------AHRGLRDNAFIEAHTKGFDEALAVADADCADPATV 277 Query: 341 IAESGVSREDIEKLAQVYVKGKAVIATWGMGLTQHKYAVATIQLLSNMMMLRGNIGRQGA 400 + +D+ + + + V+ + MG+ Q + + N + G IG+ G Sbjct: 278 ARLCKIDLKDLLAFYEAFANTEKVVTGFSMGVNQSSAGTDKVNSIINCHLATGRIGKPGM 337 Query: 401 GLCPVRGHSNVQGDRTVGIDEKPTPAFLD-------RLEQVFGFKAPR---HHGNDTVGS 450 G + G N G R VG A +D ++ Q F +++PR G V Sbjct: 338 GPFSITGQPNAMGGREVGGLANMLAAHMDLDQPLHRQIVQDF-WQSPRIADKPGLKAVDM 396 Query: 451 VMAMLEGRTKVFIGLGGNFAMATPDTPRTFEAMRSCNLTVH--ITTKLNRSHLVHGKDAL 508 A+ EGR K + N ++ PD A++ C L V + T+ + + L H + Sbjct: 397 FRAIEEGRIKAVWIMATNPMVSLPDADLVQRALQKCELVVSSDVITQTDTNALAH----V 452 Query: 509 ILPTMGRTEID 519 +LP + E D Sbjct: 453 LLPALAWGEKD 463 >gi|300313158|ref|YP_003777250.1| anaerobic dehydrogenase family protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 59.7 bits (143), Expect = 9e-10, Method: Compositional matrix adjust. Identities = 67/268 (25%), Positives = 109/268 (40%), Gaps = 43/268 (16%) Query: 111 RLTHPM--VYDAATDKYKKIDWSAAFELMAKHLN--ALPDPD--MADFYVSGRASNEAAF 164 RL HPM V K+++I W A +A+ L AL DP + Y + Sbjct: 61 RLLHPMRRVGKKGEGKFERISWDEAIATIAQRLGQIALRDPQAILPYSYAGTMGQVQGES 120 Query: 165 LFQLFVRQYGTNNFPDCSNMCHEPTSVGLPGTVGIGKGTVLLEDFDHCDTLLLFGQNPAT 224 + F + G + +C G T+G G L E DH +L++G NP Sbjct: 121 MAGRFFHRLGASQLE--RTICASAGGAGYRYTIGARVGADL-EQVDHAKLILIWGGNPIA 177 Query: 225 NHPRMMGELRHASKRGATIVAINPLKERGLERFADPQSKVEMLTMGSTRISSMFIHPTLG 284 ++ ++ A + GA ++AI+P + E+ + +L G Sbjct: 178 SNLHFWTRVQTAKRNGARLIAIDPYRSLTAEKC---HQHIALLP---------------G 219 Query: 285 GDLALIKGVIKRTIELDDAAKAAGAERVIDVKFIEEHTAGFEEFAAQARAESWDDI-IAE 343 D AL G++ I +E ++D +I HT GFE+ + R W AE Sbjct: 220 TDAALALGMMHVLI----------SEDLLDHDYIARHTLGFEQL--RERVAEWTPARTAE 267 Query: 344 -SGVSREDIEKLAQVYVKGKAVIATWGM 370 G+S E++ LA+ Y G+ +A G+ Sbjct: 268 VCGISAEEVLNLAREY--GRVALAGEGV 293 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,537,051 Number of extensions: 111810 Number of successful extensions: 275 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 267 Number of HSP's successfully gapped: 5 Length of query: 775 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 682 Effective length of database: 1,179,515 Effective search space: 804429230 Effective search space used: 804429230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)