BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0141.p (391 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 691 0.0 gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protei... 488 e-139 gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protei... 451 e-128 gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protei... 301 5e-83 gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protei... 290 1e-79 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 691 bits (1784), Expect = 0.0, Method: Compositional matrix adjust. Identities = 330/394 (83%), Positives = 359/394 (91%), Gaps = 4/394 (1%) Query: 1 MYFCGFEAAWLDHPFWKNKFLLKNEADLRQAHGSGIEHCWIDPERGLDVA---EAAAEAG 57 MYFCGFEAAWLDHPFWKNKFLLKN+++LRQAH SGI +CWID ERGLDVA EAA A Sbjct: 14 MYFCGFEAAWLDHPFWKNKFLLKNDSELRQAHASGIAYCWIDTERGLDVAQEEEAADSAA 73 Query: 58 PDEAAAPAEPSPELPPGPDIDTTLMEAEALRENAKKATKAMFSEARLGKALDMERCVTVV 117 P + P+ P PE P ID L++A ALRE AKKATKAMFSEAR+GK LDMERCV+VV Sbjct: 74 PLQETEPS-PEPEHAAAPPIDAVLLDAMALRETAKKATKAMFSEARMGKVLDMERCVSVV 132 Query: 118 EEIAGAVLQSPNGTLSVLRLKLADEYTYLHSVAVCALMVALGRTLGLDEKACREAGLGGF 177 EEIAG+V++SPNG LSVLRLKLADEYTYLHSVAVCALMVALGRTLGLDE ACREAGLGGF Sbjct: 133 EEIAGSVIKSPNGALSVLRLKLADEYTYLHSVAVCALMVALGRTLGLDETACREAGLGGF 192 Query: 178 LHDVGKAFLPHELLNKPGKLTDEEFTQIKKHPELGYKYLSQDPNVPDYARDVCLHHHEKM 237 LHDVGKAF+PHELLNKPGKL+DEEF+QIKKHPELGYKYLSQDPNVPDYARDVCLHHHEK+ Sbjct: 193 LHDVGKAFIPHELLNKPGKLSDEEFSQIKKHPELGYKYLSQDPNVPDYARDVCLHHHEKI 252 Query: 238 DGHGYPELLRSGQISLYARMAAICDVYDALTSDRPYKRAWDPAEAISSMASWTGHFDRAM 297 DG+GYPE LR+G I+LY+RMAAICDVYDALTSDRPYKRAWDPAEAIS+MASWTGHFDRAM Sbjct: 253 DGNGYPEGLRAGAITLYSRMAAICDVYDALTSDRPYKRAWDPAEAISAMASWTGHFDRAM 312 Query: 298 FLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFRYNDDMEMSDTEILDLM 357 FLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVF Y+D M ++DTEILDL+ Sbjct: 313 FLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFHYDDSMTVTDTEILDLL 372 Query: 358 ATPEADTIVARGGEEWARLSHLDTMRMLSTGTPS 391 A PEADTIVARGGEEWARLSHLD M++LSTG P+ Sbjct: 373 ANPEADTIVARGGEEWARLSHLDAMQLLSTGVPT 406 >gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 488 bits (1257), Expect = e-139, Method: Compositional matrix adjust. Identities = 242/381 (63%), Positives = 285/381 (74%), Gaps = 3/381 (0%) Query: 1 MYFCGFEAAWLDHPFWKNKFLLKNEADLRQAHGSGIEHCWIDPERGLDVAEAAAEAGPDE 60 MYFCGFEA+WL HPFWK +FLL E+DL+QA SGI +CWID RG DVA AA Sbjct: 14 MYFCGFEASWLAHPFWKTRFLLSRESDLQQARASGIAYCWIDVSRGRDVAGAAPAKAEAA 73 Query: 61 AAAPA-EPSPELPPGPDIDTTLMEAEALRENAKKATKAMFSEARLGKALDMERCVTVVEE 119 AA + + S G D D A ALRE+A++ T+ MF+ AR+GK +++ +CV +VEE Sbjct: 74 AAPASPDQSAATHAGADADRA--SAVALREHAREVTRQMFNAARMGKMVELPQCVALVEE 131 Query: 120 IAGAVLQSPNGTLSVLRLKLADEYTYLHSVAVCALMVALGRTLGLDEKACREAGLGGFLH 179 IA +VL+ PNG LSVLRLKL DEYTYLHSV+VCAL++ LGRTLGL E ACREAGL G+LH Sbjct: 132 IACSVLRDPNGLLSVLRLKLNDEYTYLHSVSVCALVIGLGRTLGLSEAACREAGLSGYLH 191 Query: 180 DVGKAFLPHELLNKPGKLTDEEFTQIKKHPELGYKYLSQDPNVPDYARDVCLHHHEKMDG 239 DVGKAF+ E+LNKPGKLT EEF IK+HPELGY+YL Q P++P YA DVC HHHE++DG Sbjct: 192 DVGKAFIDDEILNKPGKLTAEEFALIKRHPELGYQYLIQTPDMPAYASDVCRHHHERLDG 251 Query: 240 HGYPELLRSGQISLYARMAAICDVYDALTSDRPYKRAWDPAEAISSMASWTGHFDRAMFL 299 GYP+ L + I+LYARM A+CDVYDALTS+RPYK WDPAEAIS MA W GHFDR + L Sbjct: 252 RGYPDALPAESITLYARMTAVCDVYDALTSERPYKHGWDPAEAISQMARWEGHFDRRLLL 311 Query: 300 AFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFRYNDDMEMSDTEILDLMAT 359 AFVKTLGIYPVGS+V+LESG SG VIRQN DLTRPVLKV E + +LDLMA Sbjct: 312 AFVKTLGIYPVGSLVRLESGVSGVVIRQNPADLTRPVLKVLHLQGPHETTIGGVLDLMAW 371 Query: 360 PEADTIVARGGEEWARLSHLD 380 P DTI+ RG EEW RL D Sbjct: 372 PRTDTIIGRGDEEWTRLRQQD 392 >gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 408 Score = 451 bits (1160), Expect = e-128, Method: Compositional matrix adjust. Identities = 207/379 (54%), Positives = 275/379 (72%), Gaps = 6/379 (1%) Query: 1 MYFCGFEAAWLDHPFWKNKFLLKNEADLRQAHGSGIEHCWIDPERGLDVAEAAAEAGPDE 60 MYF GF+ AW+D PFW+ FL++ +++ +A +G +CWID ++G+D+A A A+ P+ Sbjct: 16 MYFSGFDGAWIDTPFWRTSFLVRKASEIARARAAGYTYCWIDTDQGIDLAGAEADRLPET 75 Query: 61 AAAPAEPSPELPPGPDIDTTLMEAEALRENAKKATKAMFSEARLGKALDMERCVTVVEEI 120 PE + L +A L ENAK+ T+ MF +ARLGK LD+E C ++VEEI Sbjct: 76 ------EEPEAAGSSSSRSELDDALRLYENAKQTTQQMFKQARLGKTLDVENCKSLVEEI 129 Query: 121 AGAVLQSPNGTLSVLRLKLADEYTYLHSVAVCALMVALGRTLGLDEKACREAGLGGFLHD 180 A +V + N LS++RLK DEYTYLHS+AVC LMVAL RTLG+D+ AC+EAGL G+LHD Sbjct: 130 ATSVARDSNALLSIIRLKRVDEYTYLHSIAVCTLMVALARTLGMDDIACKEAGLAGYLHD 189 Query: 181 VGKAFLPHELLNKPGKLTDEEFTQIKKHPELGYKYLSQDPNVPDYARDVCLHHHEKMDGH 240 VGKAF+P E+LNKP +L+++E+ I++HP LGY YL VPDY DVCLHHHEK+DG Sbjct: 190 VGKAFIPLEILNKPDRLSEQEYKIIQRHPALGYDYLKGFSGVPDYVLDVCLHHHEKIDGS 249 Query: 241 GYPELLRSGQISLYARMAAICDVYDALTSDRPYKRAWDPAEAISSMASWTGHFDRAMFLA 300 GYP + QIS+Y+RMAA+CDVYDA+TS+RPYK WDPAE++S MASW GHFDR +FLA Sbjct: 250 GYPSRHQGNQISVYSRMAAVCDVYDAVTSERPYKSGWDPAESLSRMASWQGHFDRPIFLA 309 Query: 301 FVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFRYNDDMEMSDTEILDLMATP 360 FVK LGIYP+GSIVKL+SGR+ VIRQN DLT+PV+K F +E+ ++EI+DL A Sbjct: 310 FVKALGIYPIGSIVKLQSGRTALVIRQNHVDLTKPVVKAFVTTPSIEVVESEIIDLSAPG 369 Query: 361 EADTIVARGGEEWARLSHL 379 + D I R G++W + + L Sbjct: 370 QTDAIAERSGQDWLKSNGL 388 >gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 301 bits (772), Expect = 5e-83, Method: Compositional matrix adjust. Identities = 156/381 (40%), Positives = 219/381 (57%), Gaps = 9/381 (2%) Query: 1 MYFCGFEAAWLDHPFWKNKFLLKNEADLRQAHGSGIEHCWIDPERGLDVAEAAAEAG--- 57 MY F AW P W+ FLL+ + DL S I ID +G DV AE G Sbjct: 14 MYIQSFSGAWRRPPAWRKPFLLEKDKDLLALQASPIREIVIDIAKGADVQTDEAEQGNGV 73 Query: 58 -----PDEAAAPAEPSPELPPGPDIDTTLMEAEALRENAKKATKAMFSEARLGKALDMER 112 + A+ PE DI A + +++K MFS+AR+GK +D++ Sbjct: 74 ESSLGKNAEIEVAQALPEAGKRTDIQAERERAARIISSSEKTVLTMFSQARMGKTIDVKE 133 Query: 113 CVTVVEEIAGAVLQSPNGTLSVLRLKLADEYTYLHSVAVCALMVALGRTLGLDEKACREA 172 +V EI +V ++ + +S++RLK AD YTY+HSVAVCA+M++L LGL + ++A Sbjct: 134 ASDLVREINASVSRNADAMISLVRLKTADNYTYMHSVAVCAMMISLADQLGLSAQERQQA 193 Query: 173 GLGGFLHDVGKAFLPHELLNKPGKLTDEEFTQIKKHPELGYKYLSQDPNVPDYARDVCLH 232 G+ G LHD+GK +P +LNKP KLT+EEF ++ HP G++ L + P + D DV LH Sbjct: 194 GMAGLLHDIGKMTVPPSILNKPSKLTEEEFVSVQSHPLAGHRLLQELPGIGDAPLDVSLH 253 Query: 233 HHEKMDGHGYPELLRSGQISLYARMAAICDVYDALTSDRPYKRAWDPAEAISSMASWTGH 292 HHEKMDG GYP L IS+ ARM AICDVYDA+TSDRPYK WDP+ +I MA GH Sbjct: 254 HHEKMDGTGYPYKLSGRDISMTARMGAICDVYDAITSDRPYKSGWDPSRSIQHMAQSKGH 313 Query: 293 FDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFRYNDDMEMSDTE 352 FD + AFVK +GIYPVGS V +++G V+ Q L P++++F Y+ + +++ T Sbjct: 314 FDFDVLQAFVKAVGIYPVGSCVLMQTGHLAMVLEQRPDSLLAPLVRIF-YSTNKKIAVTP 372 Query: 353 ILDLMATPEADTIVARGGEEW 373 L + + A E+W Sbjct: 373 TLIDLRRSANKIVGAVDPEKW 393 >gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 401 Score = 290 bits (742), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 154/361 (42%), Positives = 213/361 (59%), Gaps = 5/361 (1%) Query: 1 MYFCGFEAAWLDHPFWKNKFLLKNEADLRQAHGSGIEHCWIDPERGLDVAEAAAE---AG 57 MY W+ HPF + F ++ DL+ I+ I+ +G DV E A Sbjct: 14 MYIHSIPGGWISHPFRRKSFKVETLEDLQTLRDCPIDAITINTAKGRDVTREELEEDDAD 73 Query: 58 PDEAAAPAEPSPELPPGPDIDTTLMEAEALR--ENAKKATKAMFSEARLGKALDMERCVT 115 D AAP + P DT + A R ++K MFSEAR+GKA+D++ Sbjct: 74 QDAPAAPPADAANRNPASRTDTAIERERAQRIISSSKSTVLHMFSEARMGKAIDVKDAAE 133 Query: 116 VVEEIAGAVLQSPNGTLSVLRLKLADEYTYLHSVAVCALMVALGRTLGLDEKACREAGLG 175 +V EI +V ++ + +S+ RLK D+YTYLHSVAVCA+M++L LG+ + ++AG+ Sbjct: 134 LVTEITASVSRNADALISLARLKTTDDYTYLHSVAVCAMMISLANQLGMTPQQTKQAGMA 193 Query: 176 GFLHDVGKAFLPHELLNKPGKLTDEEFTQIKKHPELGYKYLSQDPNVPDYARDVCLHHHE 235 G LHDVGK +P ++LNKP KLT+EEF ++ H G+ L Q + + A DV LHHHE Sbjct: 194 GLLHDVGKMAVPLDILNKPAKLTEEEFASVRMHTVQGHGILQQIEGIGEVALDVSLHHHE 253 Query: 236 KMDGHGYPELLRSGQISLYARMAAICDVYDALTSDRPYKRAWDPAEAISSMASWTGHFDR 295 K+DG GYP L++ IS+ A+M A+CDVYDA+TS+RPYK WDPA +I MA+ GHFD Sbjct: 254 KIDGSGYPFNLKADSISIMAKMGAVCDVYDAITSNRPYKAGWDPARSIRHMAASAGHFDP 313 Query: 296 AMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFRYNDDMEMSDTEILD 355 AFVK +GIYP GS V L+SGR G V+ Q L P +KVF + ++EI+D Sbjct: 314 ITMQAFVKAIGIYPTGSCVMLQSGRLGVVVDQRPDQLLTPRVKVFYSTANRMPLESEIID 373 Query: 356 L 356 L Sbjct: 374 L 374 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,175,520 Number of extensions: 46345 Number of successful extensions: 135 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 126 Number of HSP's successfully gapped: 10 Length of query: 391 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 304 Effective length of database: 1,207,925 Effective search space: 367209200 Effective search space used: 367209200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)