BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0139.p (493 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum... 964 0.0 gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum s... 149 6e-37 gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirill... 103 3e-23 gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) ... 102 1e-22 gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herb... 90 3e-19 >gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 964 bits (2491), Expect = 0.0, Method: Compositional matrix adjust. Identities = 471/492 (95%), Positives = 481/492 (97%) Query: 1 MAYLLALDQGTSSSRSIIFDQSGAIVATAQQEFPQIFPQPGWVEHDPMDLWQSQLQTAQK 60 MAYLLALDQGTSSSRSIIFDQSGAIVATAQQEFPQIFPQPGWVEHDPMDLW SQLQTAQK Sbjct: 1 MAYLLALDQGTSSSRSIIFDQSGAIVATAQQEFPQIFPQPGWVEHDPMDLWHSQLQTAQK 60 Query: 61 VLAQSGIKAEELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALRDRGLGE 120 VLAQSGIKA ELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALR+RGLGE Sbjct: 61 VLAQSGIKASELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALRERGLGE 120 Query: 121 AIQKKTGLVLDPYFSGTKLRWILDNVDGLRARAERGELLFGTVDSWLVWNLTGGRLHVSD 180 AIQKKTGLVLDPYFSGTKLRWILD+VDGLRARAERGELLFGTVDSWLVWNLTGGRLHVSD Sbjct: 121 AIQKKTGLVLDPYFSGTKLRWILDHVDGLRARAERGELLFGTVDSWLVWNLTGGRLHVSD 180 Query: 181 VSNASRTMLWNIHTGAWDEELLQWLDIPAAMLPQVHASAHIYGEIDAQHLGAAVKIGGIA 240 VSNASRTMLWNIHTGAWDEELLQWL IPAAMLPQV SAH+YGEIDAQHLGAAVKIGGIA Sbjct: 181 VSNASRTMLWNIHTGAWDEELLQWLGIPAAMLPQVQPSAHLYGEIDAQHLGAAVKIGGIA 240 Query: 241 GDQQSALFGQACVRPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVDERKQYALE 300 GDQQSALFGQACVRPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVDE+KQYALE Sbjct: 241 GDQQSALFGQACVRPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVDEQKQYALE 300 Query: 301 GSVFIGGAVVQWLRDGLKAIKTSTDVEGLACTVDDSGGVVFVPSFTGLGAPYWNPGAKGA 360 GSVFIGGAVVQWLRDGLKAIKTSTDVEGLAC+V+DSGGVVFVPSFTGLGAPYWNP AKGA Sbjct: 301 GSVFIGGAVVQWLRDGLKAIKTSTDVEGLACSVEDSGGVVFVPSFTGLGAPYWNPSAKGA 360 Query: 361 IVGLSRGSTVAHIARAALESIAFQSTALLNAMVKDAVSPITELRVDGGAAANNLLLQFQA 420 IVGLSRGSTVAHIARAALESIAFQSTALLNAMV+DAVSPITELRVDGGAAANNLLLQFQA Sbjct: 361 IVGLSRGSTVAHIARAALESIAFQSTALLNAMVRDAVSPITELRVDGGAAANNLLLQFQA 420 Query: 421 DLLGIPVIRPQVTETTALGAAYLAGVATGVYRDLSELSAQWRMERTFEPRFTREQAQERI 480 DLLGIPVIRPQVTETTALGAAYLAG+ATGVYRDLSELS+QWRME TFEP R+QAQ RI Sbjct: 421 DLLGIPVIRPQVTETTALGAAYLAGIATGVYRDLSELSSQWRMEHTFEPSIGRDQAQARI 480 Query: 481 HAWEAAIRQVSA 492 HAWEAAIRQVSA Sbjct: 481 HAWEAAIRQVSA 492 >gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 149 bits (375), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 130/472 (27%), Positives = 209/472 (44%), Gaps = 28/472 (5%) Query: 3 YLLALDQGTSSSRSIIFDQSGAIVATAQQEFPQIFPQPGWVEHDPMDLWQSQLQTAQKVL 62 Y+L +D GT+S++S++F G +VA +E+P + +PG E DP + + L + + Sbjct: 9 YMLGVDIGTTSTKSVVFTLDGKVVAQHAEEYPVLCTEPGMAEQDPEQIVAAALASIAGAV 68 Query: 63 AQSGIKAEELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALRDRGLGEAI 122 + +E+ L + + + + A + + N++ W D RA V ++ G AI Sbjct: 69 KAAKAAPQEIALLSFSAAMHSVIALD-AENRLLSNSITWGDIRASVWAERIKHEHDGNAI 127 Query: 123 QKKTGLVLDPYFSGTKLRWILDNVDGLRARAERGELLFGTVDSWLVWNLTGGRLHVSDVS 182 ++TG + P KL W+ + RA R F + +L++ L G V D S Sbjct: 128 YRRTGTPVHPMSPLCKLMWMRHEKPDVFHRAAR----FVGIKEYLLYQLFGQW--VVDHS 181 Query: 183 NASRTMLWNIHTGAWDEELLQWLDIPAAMLPQVHASAHIYGEID---AQHLGAAVKIGGI 239 AS T ++N+ AWD+ L L + LP + H + AQ LG +V I Sbjct: 182 IASATGMFNLRELAWDQGALALLGVRPDQLPTPVPTTHRLPALSSEMAQRLGLSVDTPFI 241 Query: 240 AGDQQSAL--FGQACVRPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVDERKQY 297 G L G + G T GT M D+ T G + C K + Sbjct: 242 IGANDGVLSNLGVNAIEIGHVAVTIGTSGAMRT-VIDEPRTDPQGRLF---CYALTEKHW 297 Query: 298 ALEGSVFIGGAVVQWLRDGLKAIKTST-------DVEGLACTVDD----SGGVVFVPSFT 346 + G V GG + +W+RD L + + E L + + G++F P Sbjct: 298 VVGGPVNNGGNIFRWVRDELATAEAAAAREEGVDPYEALTRIAEKVRPGAEGLLFHPFMA 357 Query: 347 GLGAPYWNPGAKGAIVGLSRGSTVAHIARAALESIAFQSTALLNAMVKDAVSPITELRVD 406 G AP WN +G+ GL+ H+ RAALE + F ++L A+ ++ V P + Sbjct: 358 GERAPLWNADLRGSFFGLALHHGKHHMIRAALEGVIFNLYSILPAL-EELVGPTKRMMAT 416 Query: 407 GGAAANNLLLQFQADLLGIPVIRPQVTETTALGAAYLAGVATGVYRDLSELS 458 GG A + L Q AD+ V+ P+ E++ LGAA L A G+ LS +S Sbjct: 417 GGFARSALWRQMMADIFNREVVVPESVESSCLGAAVLGAWALGLVPSLSVIS 468 >gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 103 bits (258), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 112/455 (24%), Positives = 192/455 (42%), Gaps = 23/455 (5%) Query: 1 MAYLLALDQGTSSSRSIIFDQSGAIVATAQQEFPQIFPQPGWVEHDPMDLWQSQLQTAQK 60 M+YL +D GTS + ++ D+ ++A+ P P W E P W + L Sbjct: 1 MSYL-GIDLGTSEVKLVLTDEDSNVIASTSARLRVDNPHPLWSEQAPQSWWNATLDAIAA 59 Query: 61 VLAQSGIKAEELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALRDRGLGE 120 + Q+ + L +G++ Q + +R G + A++W D RA C L L Sbjct: 60 LRTQAPQAFQALRGIGLSGQMHGATLLDR-NGNVLRPAILWNDMRAHAECVEL--EALVP 116 Query: 121 AIQKKTGLVLDPYFSGTKLRWILDNVDGLRARAERGELLFGTVDSWLVWNLTGGRLHVSD 180 TG P F+ KL W L + RA LL +L W LTG VS+ Sbjct: 117 DAVAITGNRAMPGFTAPKLLW-LSKYEPAVYRAIDKVLL---PKDFLGWKLTGE--FVSE 170 Query: 181 VSNASRTMLWNIHTGAWDEELLQWLDIPAAMLPQVHASAHIYGEIDAQ-----HLGAAVK 235 +S+A+ T+ ++ W E +L + + +P++ + + G++ + + V Sbjct: 171 MSDAAGTLWLDVARRDWSERMLGATGLDRSHMPRLVEGSAVAGQLRDELRREWGISGPVV 230 Query: 236 IGGIAGDQQSALFGQACVRPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVDERK 295 + G AGD ++ G +R G A + G+ + T + G+ + C + Sbjct: 231 VAGGAGDNAASAVGIGVIRAGDAFLSLGSSGVLFAATAHHAPNAQQGVHAFCHCLPGQWH 290 Query: 296 QYALEGSVFIGGAVVQWLRDGLKAIKTSTDVEGLA--CTVDDSGGVVFVPSFTGLGAPYW 353 Q ++ + A + WL G+ A + V G + +F+P G P+ Sbjct: 291 QMSV---ILSAAASLHWL-SGVTARPVAELVSGAERLSAGQQAQAPLFLPYLNGERTPHN 346 Query: 354 NPGAKGAIVGLSRGSTVAHIARAALESIAFQSTALLNAMVKDAVSPITELRVDGGAAANN 413 + AKG + G++ AH+A A +E +AF + A A ++ A + + GG + + Sbjct: 347 DAAAKGVLFGMTPAHESAHLAYAVMEGVAF-AMADGYAALQAAGTTLESAAFVGGGSRSA 405 Query: 414 LLLQFQADLLGIPVIRPQVTET-TALGAAYLAGVA 447 A LGIP+ R E ALGAA L +A Sbjct: 406 FWGVLCATALGIPLRRHAGAEVGAALGAARLGRLA 440 >gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) kinase [Herbaspirillum seropedicae SmR1] Length = 490 Score = 102 bits (253), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 130/499 (26%), Positives = 219/499 (43%), Gaps = 42/499 (8%) Query: 5 LALDQGTSSSRSIIFDQSGAIVATAQQEFPQIFPQPGWVEHDPMDLWQSQLQTAQKVLAQ 64 L +D GTS ++++ D G++VA A P P W E P D WQ+ T K+ Q Sbjct: 3 LGIDLGTSEVKAVVIDAQGSLVALAGSTLNVARPHPRWSEQAPADWWQATCDTVAKLRTQ 62 Query: 65 SGI-KAEELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALRDRGLGEAIQ 123 G + + A+G++ Q V+ + + + A++W D R+ CA L R + Sbjct: 63 LGSERFGSIRAIGLSGQMHGAVLLDE-RDEVLRPAILWSDSRSAPECAELESR--APRLH 119 Query: 124 KKTGLVLDPYFSGTKLRWILDNVDGLRARAERGELLFGTV---DSWLVWNLTGGRLHVSD 180 G + P F+ KL W+ + L AR TV WL +TG + VSD Sbjct: 120 GIAGNLAMPGFTAPKLLWVARHEPQLFAR-------IATVLLPKDWLRLKMTGRK--VSD 170 Query: 181 VSNASRTMLWNIHTGAWDEELLQWLDIPAAMLPQVHASAHIYGEI-----DAQHLGAAVK 235 S+A+ T+ ++ W +ELL + +P + + + G + A L + V Sbjct: 171 PSDAAGTLWLDVEGRNWSDELLAASGMRRDQMPALVDGSAVSGSLLPEVAQAWGLRSDVI 230 Query: 236 IGGIAGDQQSALFGQACVRPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVDERK 295 + G AGD ++ G V+PG + GT + + D+ + G A C ++ Sbjct: 231 VAGGAGDGAASAVGIGAVKPGDGFLSLGTSGVLFV-VNDRF-RPNPGRAIHAFCHTLPQR 288 Query: 296 QYALEGSVFIGGA-VVQWLRDGLKAIKTSTDVEGLACTVDD------SGGVVFVPSFTGL 348 + + SV + A ++W S D + L ++ + +F+P +G Sbjct: 289 WHQM--SVMLSAASCLRWF-----CRLCSVDEKSLLAEIEQLDEQACNNAPLFLPYLSGE 341 Query: 349 GAPYWNPGAKGAIVGLSRGSTVAHIARAALESIAFQSTALLNAMVKDAVSPITELRVDGG 408 P+ +P A G GL+ A + A LE +AF L+A+ + A + + EL + GG Sbjct: 342 RTPHNDPYATGVFHGLTPEHQRAALGYAVLEGVAFGMVDGLDAL-RAAGTDVAELSLVGG 400 Query: 409 AAANNLLLQFQADLLGIPVIRPQVTET-TALGAAYLAGVATGVYRDLSELSAQWRMERTF 467 A + Q AD L + ++ +E ALGAA LA +A G D +E+ + + + Sbjct: 401 GARSAYWAQLLADALQVRIVTHVGSEAGGALGAARLAWLADG--GDEAEVCRKPAQQALY 458 Query: 468 EPRFTREQA-QERIHAWEA 485 +P R A Q R+ + A Sbjct: 459 QPDPARHAALQARLQRYRA 477 >gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herbaspirillum seropedicae SmR1] Length = 548 Score = 90.1 bits (222), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 125/506 (24%), Positives = 191/506 (37%), Gaps = 73/506 (14%) Query: 3 YLLALDQGTSSSRSIIFDQSGAIVATAQQEFPQIF--PQPGWVEHDPMDLWQSQLQTAQK 60 YL+ +D GT S+R+ +FD G ++A+A+ + +F + VE +W++ Q + Sbjct: 4 YLIGIDVGTGSARAGVFDPQGQLLASARHDI-DLFRDERRARVEQSSAQIWEAVCQAVRA 62 Query: 61 VLAQSGIKAEELTALGITNQRETTVVWERA----TGKPVYNAVVWQDRRAEVVCAALRDR 116 + +SGI + +G+ V A G P + +VW D RA + A R Sbjct: 63 AVTKSGIDPAAVAGIGVDATCSLVVQGASAGVGDPGHPERDVIVWMDHRA--LEQAQRIN 120 Query: 117 GLGEAIQKKTGLVLDPYFSGTKLRWILDNVDGLRARAERGELLFGTVDSWLVWNLTGGRL 176 +G A+ G V+ P KL W+ +N+ + A A F + +L W TG Sbjct: 121 RMGHAVLSYVGGVISPEMETPKLLWLKENLPEVYASAAH----FFDLTDFLTWKATGSLQ 176 Query: 177 HVSDVSNASRTMLWNIHTGAWDEELLQWL---DIPAAMLPQVHASAHIYG--------EI 225 S T L H WD + + D+ A ++ A G + Sbjct: 177 RSSCTVTCKWTYL--AHEAGWDASYFRQIGLGDLAEAGFARIGAEVVWPGTPLAGGLTQT 234 Query: 226 DAQHLGAAVKIGGIAG--DQQSALFGQACVRPGMAKNTYGTGCFMLLHTGDQCA-TSHNG 282 AQ +G I AG D + G R G C + C TSH Sbjct: 235 AAQAMGLRPGIAVAAGLIDAHAGGVGTVAARGGQGD---AAACMAYVFGTSSCTMTSHAD 291 Query: 283 LISTAACQVDERKQYAL-----EGSVFIGGAVV-------------------------QW 312 + K A EG GA + QW Sbjct: 292 PVLVPGVWGPYYKAMAPGMWLNEGGQSAAGAAIDHLLRLHPATPQAREEAARAQMELPQW 351 Query: 313 L-RDGLKAIKTSTDVEGLACTVDDSGGVVFVPSFTGLGAPYWNPGAKGAIVGLSRGSTVA 371 L + L A+ ++ LA G + VP F G +P +P A+ A+VGL + Sbjct: 352 LAQQALAAVDHPSEAVWLA------GQLTVVPEFLGNRSPLADPQARAALVGLGMEHDID 405 Query: 372 HIAR---AALESIAFQSTALLNAMVKDAVSPITELRVDGGAAANNLLLQFQADLLGIPVI 428 + A L S+ + ++ A V + + V GGA + L Q AD G+PV Sbjct: 406 SLVALYVAGLCSLGYGLRQIIEAQAACGVR-VQSISVSGGAGVHPLTRQLLADATGMPVE 464 Query: 429 RPQVTETTALGAAYLAGVATGVYRDL 454 E LG+A LA VA G++ DL Sbjct: 465 ITASPEPVLLGSAMLAAVAAGLHADL 490 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,578,629 Number of extensions: 66716 Number of successful extensions: 225 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 212 Number of HSP's successfully gapped: 6 Length of query: 493 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 404 Effective length of database: 1,198,455 Effective search space: 484175820 Effective search space used: 484175820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)