BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0131.p (510 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310299|ref|YP_003774391.1| glycerol-3-phosphate dehydrogen... 965 0.0 gi|300314008|ref|YP_003778100.1| glycerol-3-phosphate dehydrogen... 614 e-177 >gi|300310299|ref|YP_003774391.1| glycerol-3-phosphate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 510 Score = 965 bits (2494), Expect = 0.0, Method: Compositional matrix adjust. Identities = 485/510 (95%), Positives = 494/510 (96%) Query: 1 MTLEYDLLVVGGGINGAGIARDAAGRGLRVLLCEQHDLAQHTSSASTKLIHGGLRYLEYY 60 MTLEYDLLVVGGGINGAGIARDAAGRGLRVLLCEQHDLAQHTSSASTKLIHGGLRYLEYY Sbjct: 1 MTLEYDLLVVGGGINGAGIARDAAGRGLRVLLCEQHDLAQHTSSASTKLIHGGLRYLEYY 60 Query: 61 EFKLVRKALQEREVLLRSAPHIIWPLRFVMPHDANQRPAWMIRAGLFMYDYLAKREILPA 120 EFKLVRKALQEREVLLRSAPHIIWPLRFVMPHDANQRPAWMIRAGLFMYD+LAKREILP Sbjct: 61 EFKLVRKALQEREVLLRSAPHIIWPLRFVMPHDANQRPAWMIRAGLFMYDHLAKREILPP 120 Query: 121 SEGANLARHPGGQPLKGDYRKGFVYSDGWVDDARLVVLNAMDAQERGARILTRTKCISAR 180 SEGANLARH GQPLKGDYRKGFVYSDGWVDDARLVVLNAMDAQERGARILTRTKCISAR Sbjct: 121 SEGANLARHDAGQPLKGDYRKGFVYSDGWVDDARLVVLNAMDAQERGARILTRTKCISAR 180 Query: 181 REGDTWHAVLEHQHASGVQQEEVRARAIVNAAGPWAARFAQSVPQAKTGRSLRLIKGSHI 240 REGD WHAVLEHQ GVQQEEVRARAIVNAAGPWAARFAQ+VPQAK+GRSLRLIKGSHI Sbjct: 181 REGDIWHAVLEHQREGGVQQEEVRARAIVNAAGPWAARFAQAVPQAKSGRSLRLIKGSHI 240 Query: 241 VVKRLFDHPYAYIFQNPDKRIIFAIPYQEEFTLIGTTDLEYHGDAGKVEIDAEEIDYLCE 300 VVKR+FDHPYAYIFQNPDKRIIFAIPYQEEFTLIGTTDLEYHGDAGKVEIDA EIDYLC+ Sbjct: 241 VVKRMFDHPYAYIFQNPDKRIIFAIPYQEEFTLIGTTDLEYHGDAGKVEIDAAEIDYLCD 300 Query: 301 MANRYFKRQIGKEDVVWTYSGVRPLLDDSAENASAITRDYLLECDDAGAPMLNVWGGKIT 360 MANRYFKRQIGKEDVVWTYSGVRPLLDDSAENASAITRDYLLECDDAGAPMLNVWGGKIT Sbjct: 301 MANRYFKRQIGKEDVVWTYSGVRPLLDDSAENASAITRDYLLECDDAGAPMLNVWGGKIT 360 Query: 361 TYRKLAEEALFMLAPHLGASSQPWTAGAPLPGGDLEQPGKMVARYDFDAYLRKLQARYPW 420 TYRKLAEEAL MLAPHLGASSQ WTAGAPLPGGDLEQPGKMVARYDFDAYLRK QAR+PW Sbjct: 361 TYRKLAEEALAMLAPHLGASSQSWTAGAPLPGGDLEQPGKMVARYDFDAYLRKFQARHPW 420 Query: 421 LPAGLAHRYARSYGSRVDQIIGQADSLAALGEELTPGLYEAEARYLVQHEWARGADDILW 480 LPA LAHRYARSYGSRV+QIIGQADSLAALGEEL+PGLYEAEARYLVQHEWAR ADDILW Sbjct: 421 LPAELAHRYARSYGSRVEQIIGQADSLAALGEELSPGLYEAEARYLVQHEWARSADDILW 480 Query: 481 RRSKLGLHCGPQHRGRLEAWLANLPDATPL 510 RRSKLGLHCGPQHRGRLEAWLA+LPD L Sbjct: 481 RRSKLGLHCGPQHRGRLEAWLASLPDHATL 510 >gi|300314008|ref|YP_003778100.1| glycerol-3-phosphate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 534 Score = 614 bits (1584), Expect = e-177, Method: Compositional matrix adjust. Identities = 315/501 (62%), Positives = 383/501 (76%), Gaps = 6/501 (1%) Query: 4 EYDLLVVGGGINGAGIARDAAGRGLRVLLCEQHDLAQHTSSASTKLIHGGLRYLEYYEFK 63 +YD+LVVGGGINGAGIARDAAGRGL V LCEQHDLAQHTSS+S+KLIHGGLRYLE+YEF Sbjct: 14 QYDVLVVGGGINGAGIARDAAGRGLSVALCEQHDLAQHTSSSSSKLIHGGLRYLEHYEFG 73 Query: 64 LVRKALQEREVLLRSAPHIIWPLRFVMPHDANQRPAWMIRAGLFMYDYLAKREILPASEG 123 LVRKAL EREVLLRSAPHI+WPLRFVMPHD QRPAW+IR G+F+YD+LA+RE+LP S Sbjct: 74 LVRKALIEREVLLRSAPHIMWPLRFVMPHDQGQRPAWLIRLGMFLYDHLARRELLPGSRA 133 Query: 124 ANLARHPGGQPLKGDYRKGFVYSDGWVDDARLVVLNAMDAQERGARILTRTKCISARREG 183 +L RH G PLK + +GFVYSDGWVDDARLVVLNAMDA E+GA I+TRT+C S R+ Sbjct: 134 LDLRRHASGAPLKAQFTRGFVYSDGWVDDARLVVLNAMDAAEKGAHIMTRTRCESLTRQD 193 Query: 184 DTWHAVLEHQHASGVQQEEVRARAIVNAAGPWAARFAQSVPQAKTGRSLRLIKGSHIVVK 243 + W AVL + +Q VRAR +VNAAGPW A+F + +S+RL+KGSHIVV Sbjct: 194 NGWQAVLRREDGHAMQ---VRARCVVNAAGPWVAQFLGGAAHVHSSKSVRLVKGSHIVVP 250 Query: 244 RLFDHPYAYIFQNPDKRIIFAIPYQEEFTLIGTTDLEYHGDAGKVEIDAEEIDYLCEMAN 303 RLF+H +AYIFQNPD RI+FAIPY+++FTLIGTTD+EY G V I A+E YLC++AN Sbjct: 251 RLFEHDHAYIFQNPDGRIVFAIPYEQDFTLIGTTDIEYQGRVEDVAISADETAYLCQLAN 310 Query: 304 RYFKRQIGKEDVVWTYSGVRPLLDDSAENASAITRDYLLECDDAGAPMLNVWGGKITTYR 363 RYF++ I +DVVW+YSGVRPLL+D+A +ASA+TRDY L+ + GAP+L+++GGKITT+R Sbjct: 311 RYFEKPITPDDVVWSYSGVRPLLEDAAADASAVTRDYQLDLNLQGAPLLSIFGGKITTFR 370 Query: 364 KLAEEALFMLAPHLGASSQPWTAGAPLPGGDL--EQPGKMVARYDFDAYLRKLQARYPWL 421 KLAEEA+ M+AP LG WT LPGGDL QP R FD Y++ LQ +YPWL Sbjct: 371 KLAEEAVDMIAPVLGNQRGAWTEHGCLPGGDLYGAQPSNRSVR-QFDQYVQGLQQQYPWL 429 Query: 422 PAGLAHRYARSYGSRVDQIIGQADSLAALGEELTPGLYEAEARYLVQHEWARGADDILWR 481 PA L RYAR+YGSR ++ ++ +GEE+ GLY AE YL++HEWA A DILWR Sbjct: 430 PAALLQRYARAYGSRTHTLLEGRQNVEEMGEEILDGLYAAEVDYLLRHEWASNAADILWR 489 Query: 482 RSKLGLHCGPQHRGRLEAWLA 502 RSKLGLH L+AWLA Sbjct: 490 RSKLGLHFPKGSEAVLDAWLA 510 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,702,891 Number of extensions: 75721 Number of successful extensions: 195 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 193 Number of HSP's successfully gapped: 2 Length of query: 510 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 421 Effective length of database: 1,198,455 Effective search space: 504549555 Effective search space used: 504549555 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)