BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0119.p (356 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310289|ref|YP_003774381.1| dibenzothiophene desulfurizatio... 713 0.0 gi|300314032|ref|YP_003778124.1| acyl-CoA dehydrogenase [Herbasp... 358 e-100 gi|300314033|ref|YP_003778125.1| acyl-CoA dehydrogenase [Herbasp... 346 2e-96 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 58 1e-09 gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbasp... 57 2e-09 >gi|300310289|ref|YP_003774381.1| dibenzothiophene desulfurization flavin reductase [Herbaspirillum seropedicae SmR1] Length = 419 Score = 713 bits (1841), Expect = 0.0, Method: Compositional matrix adjust. Identities = 348/356 (97%), Positives = 354/356 (99%) Query: 1 VEEIEAYSQSGLWAINVPRAFGGAEVSYATLAEVIAIISAADPSLGQITQNHLGFVAAVR 60 VEEIEAYSQSGLWAINVPRAFGGAEVSYATLAEVIAIISAADPSLGQITQNHLGFVAAVR Sbjct: 64 VEEIEAYSQSGLWAINVPRAFGGAEVSYATLAEVIAIISAADPSLGQITQNHLGFVAAVR 123 Query: 61 TVSDPEQQQRFFADFLRGVRWGNAFSESGSKRAADFETRFVDAGDHVIVSGKKFYSTGAL 120 TVSDPEQQ+RFFADFLRGVRWGNAFSESGSKRAADFETRFVDAGDHVIV+GKKFYSTGAL Sbjct: 124 TVSDPEQQRRFFADFLRGVRWGNAFSESGSKRAADFETRFVDAGDHVIVTGKKFYSTGAL 183 Query: 121 LAHYVPIVANDEENRTWYVVAERNAPGLTVIDDWSAFGQRTTLSGTVILDQVKVPKTHLI 180 LAHYVPIVANDEENRTWYVVAERNAPGLTVIDDWSAFGQRTTLSGTVI+DQVKVPKTHLI Sbjct: 184 LAHYVPIVANDEENRTWYVVAERNAPGLTVIDDWSAFGQRTTLSGTVIIDQVKVPKTHLI 243 Query: 181 PAYKGYLEPSADGAIFQIIQAAVDVGIARAAIADTQDFVRTKSRPWIDSQRDHAWEDPYS 240 PAYKGYLEPSADGAIFQIIQAAVDVGIARAAIADTQDFVRTKSRPWIDSQRDHAWEDPYS Sbjct: 244 PAYKGYLEPSADGAIFQIIQAAVDVGIARAAIADTQDFVRTKSRPWIDSQRDHAWEDPYS 303 Query: 241 IQAVGDLQIRLHASEALLERAGRAIDVAVAAPDAENVARAQILTAEAKVLSTEIAIHATN 300 IQA+GDLQIRLHASEALLERAGRAID AVAAPDA++VA AQILTAEAKVLSTEIAIHATN Sbjct: 304 IQAIGDLQIRLHASEALLERAGRAIDAAVAAPDAQSVAHAQILTAEAKVLSTEIAIHATN 363 Query: 301 KLFELAGTRSTLGQYNLDRHWRNARTHTLHDPVRWKYAIVGNYYLNGVKPPFHAWS 356 KLFELAGTRSTLGQYNLDRHWRNARTHTLHDPVRWKYAIVGNYYLNGVKPPFHAWS Sbjct: 364 KLFELAGTRSTLGQYNLDRHWRNARTHTLHDPVRWKYAIVGNYYLNGVKPPFHAWS 419 >gi|300314032|ref|YP_003778124.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 421 Score = 358 bits (919), Expect = e-100, Method: Compositional matrix adjust. Identities = 192/355 (54%), Positives = 247/355 (69%), Gaps = 2/355 (0%) Query: 2 EEIEAYSQSGLWAINVPRAFGGAEVSYATLAEVIAIISAADPSLGQITQNHLGFVAAVRT 61 +E+E Y +SGLW I VP+ +GGA VSY TLAEVIA ISAAD S+GQI QNH V +R Sbjct: 65 DELERYVRSGLWGITVPKEYGGAGVSYGTLAEVIATISAADGSIGQIPQNHFYAVELIRV 124 Query: 62 VSDPEQQQRFFADFLRGVRWGNAFSESGSKRAADFETRFVDA--GDHVIVSGKKFYSTGA 119 Q++ FF L+G R+GNA +E G+K + D +TR G + G+KFY+TGA Sbjct: 125 NGSESQKRFFFERVLQGDRFGNALAELGTKTSHDRKTRLSRNAHGPGYRIDGRKFYATGA 184 Query: 120 LLAHYVPIVANDEENRTWYVVAERNAPGLTVIDDWSAFGQRTTLSGTVILDQVKVPKTHL 179 L A +VP D++ + R+APGLT+IDDWS FGQRTT SG+ + + V VP + Sbjct: 185 LYADWVPTSVIDDDGVQQLAIVPRHAPGLTIIDDWSGFGQRTTGSGSAVFENVHVPADAV 244 Query: 180 IPAYKGYLEPSADGAIFQIIQAAVDVGIARAAIADTQDFVRTKSRPWIDSQRDHAWEDPY 239 I + P+ G + QI AA+D+GIARAA AD FVRT+SR W+DS + A +DP Sbjct: 245 IGFATAFSRPTQIGPMAQIQHAAIDLGIARAAYADLLTFVRTRSRAWVDSGVERAVDDPL 304 Query: 240 SIQAVGDLQIRLHASEALLERAGRAIDVAVAAPDAENVARAQILTAEAKVLSTEIAIHAT 299 +I+ VGDL I+LHA+EALL RAGR +D A AAPD ++VA A I AEA+VL+TE+++ A Sbjct: 305 TIREVGDLTIQLHAAEALLRRAGRKVDAAAAAPDEDSVAAASIAVAEARVLTTEVSLAAG 364 Query: 300 NKLFELAGTRSTLGQYNLDRHWRNARTHTLHDPVRWKYAIVGNYYLNGVKPPFHA 354 +KLFELAG++STL ++NLDRHWRNARTHTLHDPVRWKY +GNY LNG KPP H Sbjct: 365 SKLFELAGSQSTLAEHNLDRHWRNARTHTLHDPVRWKYHAIGNYVLNGKKPPRHG 419 >gi|300314033|ref|YP_003778125.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 411 Score = 346 bits (888), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 190/359 (52%), Positives = 248/359 (69%), Gaps = 4/359 (1%) Query: 2 EEIEAYSQSGLWAINVPRAFGGAEVSYATLAEVIAIISAADPSLGQITQNHLGFVAAVRT 61 E+IE +SQSGLWAI VPR+ GG S+ T+AEVI +ISAADPS+GQI QNH G + + Sbjct: 53 EQIERFSQSGLWAITVPRSHGGIGASWQTVAEVIRLISAADPSIGQIPQNHFGNLNLIAR 112 Query: 62 VSDPEQQQRFFADFLRGVRWGNAFSESGSKRAADFETRFV---DAGD-HVIVSGKKFYST 117 ++ EQQ+ FFA+ L G R GNA E K D TR AGD +++G +FYST Sbjct: 113 LASQEQQRLFFAEVLDGARLGNAGPERNGKNVLDVRTRATLDRAAGDGSYLLNGTRFYST 172 Query: 118 GALLAHYVPIVANDEENRTWYVVAERNAPGLTVIDDWSAFGQRTTLSGTVILDQVKVPKT 177 GAL AH++P A D++ + V R+APG+ VIDDWS+FGQRTT SGTV+ D V+VP T Sbjct: 173 GALYAHWIPSRAIDDDGQPLVVYTRRDAPGVRVIDDWSSFGQRTTASGTVVFDNVRVPAT 232 Query: 178 HLIPAYKGYLEPSADGAIFQIIQAAVDVGIARAAIADTQDFVRTKSRPWIDSQRDHAWED 237 ++ +P+ G + Q+IQAA+D GIA AA+ DT FV T+SRP++DS + A +D Sbjct: 233 QVLALKDALQQPNNIGPVSQLIQAAIDAGIAHAAVQDTIAFVTTRSRPYVDSGLERASDD 292 Query: 238 PYSIQAVGDLQIRLHASEALLERAGRAIDVAVAAPDAENVARAQILTAEAKVLSTEIAIH 297 PY ++ +G+L +RLHA++ALLE A +D A E +A+A AEAKVLSTE+A+ Sbjct: 293 PYILRDIGNLSLRLHAADALLEDAAALLDSLPAQLSFEQMAQASAAVAEAKVLSTELALL 352 Query: 298 ATNKLFELAGTRSTLGQYNLDRHWRNARTHTLHDPVRWKYAIVGNYYLNGVKPPFHAWS 356 A+ KLFEL+G+ +TL +NLDRHWRNAR HTLHDPVRWKY VGNY LNG PP H+WS Sbjct: 353 ASEKLFELSGSAATLASHNLDRHWRNARVHTLHDPVRWKYFAVGNYALNGTPPPRHSWS 411 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 58.2 bits (139), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 82/346 (23%), Positives = 146/346 (42%), Gaps = 38/346 (10%) Query: 2 EEIEAYSQSGLWAINVPRAFGGAEVSYATLAEVIAIISAADPSLGQI--TQNHLGFVAAV 59 E ++ ++ G + VP +GGA + Y +L + I+A D + I QN L Sbjct: 38 EALKELAELGAMGMCVPEQWGGAGMDYMSLVLALEEIAAGDGATSTIVSVQNSLA-CGIT 96 Query: 60 RTVSDPEQQQRFFADFLRGVRWGN-AFSE--SGSKRAADFETRFVDAGDHVIVSG-KKFY 115 P Q++++ RG G +E +GS AA TR GD I++G K+F Sbjct: 97 EKYGSPTQKEQWLKPLARGEMLGCFCLTEPHTGSDAAA-IRTRAERDGDDFILNGTKQFI 155 Query: 116 STGALLAHYVPIVAND----EENRTWYVVAERNAPGLTVIDDWSAFGQRTTLSGTVILDQ 171 ++G + D ++ + ++V + PG V GQ + + ++LD Sbjct: 156 TSGKHAGVAIVFAVTDRSAGKKGISCFLVP-CDTPGFVVGRSEEKMGQHASDTVQIMLDN 214 Query: 172 VKVPKTHLIPAY-KGYLEPSADGAIFQIIQAAVDVGIARAAIADTQDFVRTKSR---PWI 227 +VP T L+ +GY ++ +I AA VG+ARAA + R + P I Sbjct: 215 CRVPATALLGKEGEGYRIALSNLEAGRIGIAAQSVGMARAAFEAAVSYARQRESFGVPII 274 Query: 228 DSQRDHAWEDPYSIQAVGDLQIRLHASEALLERAGRAIDVAVAAPDAENVARAQILTAEA 287 + Q + + D+ L A+ ++ RA + D P + + A++ +EA Sbjct: 275 EHQAVNFR--------LADMNTLLDAARLMVWRAAQLKD--QGRPCLKEASMAKMFASEA 324 Query: 288 --KVLSTEIAIHATNKLFELAGTRSTLGQYNLDRHWRNARTHTLHD 331 K+ S I IH G + ++R +R+ R +++ Sbjct: 325 AEKIASDAIQIH---------GGVGYTSDFPVERIYRDVRICQIYE 361 >gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 415 Score = 57.0 bits (136), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 79/355 (22%), Positives = 142/355 (40%), Gaps = 48/355 (13%) Query: 5 EAYSQSGLWAINVPRAFGGAEVS---YATLAEVIAIISAADPSLGQITQNHLGFVAAVRT 61 +A + +G +P +FGG +S +A AE + + PS + LG A V Sbjct: 51 QAMADAGFIKALLPESFGGTALSTLDFALAAEELTAVDVNVPS--ALLGTGLGLHAVVHH 108 Query: 62 VSDPEQQQRFFADFLR-GVRWGN-AFSE-------SGSKRAADFETRFVDAGDHVIVSGK 112 PEQ+QRF DF+ G R AF+E + + +T GD +++G Sbjct: 109 -GTPEQKQRFLPDFIEDGSRLAALAFTEVTGGANYDSTDPSVGVQTFARLEGDEWVINGA 167 Query: 113 KFYSTGALL-----AHYVPIVANDE-----ENRTWYVVAERNAPGLTVIDDWSAFGQRTT 162 K Y+T AH ++ + + ++ NAPG+ ++ G R T Sbjct: 168 KHYTTNGTGWDGKGAHLYSVICRTDPALPPQQSLAVIMVPGNAPGIEIVGLLDTMGHRAT 227 Query: 163 LSGTVILDQVKVPKTHLI--PAYKGYLEPSADGAIFQIIQAAVDVGIARAAIADTQDFVR 220 +S V + V+VP ++++ P + +A +I +A VG+ R A F + Sbjct: 228 ISPRVHFNNVRVPASNIVGKPGDGISMLEAAFSWTAALIGSAC-VGVMRTAFDIALRFAQ 286 Query: 221 TKSRPWIDSQRDHAWEDPYSIQAVG----DLQIRLHASEALLERAGRAIDVAVAAPDAEN 276 + R +H Q VG D+++R+ A+ L ++ +D+ Sbjct: 287 SDRRSGTIPVIEH--------QNVGFMLADIKMRIEAARYLTWKSCHQLDL--------T 330 Query: 277 VARAQILTAEAKVLSTEIAIHATNKLFELAGTRSTLGQYNLDRHWRNARTHTLHD 331 R++ L K +E+ + + G + Y L ++A L+D Sbjct: 331 EGRSRELAIITKTYCSELCVQVVYDAMRVVGVDAYTDLYPLAGLMQDALCFPLYD 385 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,151,445 Number of extensions: 47208 Number of successful extensions: 180 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 178 Number of HSP's successfully gapped: 6 Length of query: 356 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 270 Effective length of database: 1,212,660 Effective search space: 327418200 Effective search space used: 327418200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)