BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0115.p (298 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310286|ref|YP_003774378.1| branched-chain amino acid ABC t... 497 e-142 gi|300311880|ref|YP_003775972.1| amino acid ABC transporter perm... 307 8e-85 gi|300310521|ref|YP_003774613.1| branched-chain amino acid ABC t... 120 2e-28 gi|300309519|ref|YP_003773611.1| branched-chain amino acid ABC t... 120 2e-28 gi|300311114|ref|YP_003775206.1| branched-chain amino acid ABC t... 94 2e-20 >gi|300310286|ref|YP_003774378.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 298 Score = 497 bits (1279), Expect = e-142, Method: Compositional matrix adjust. Identities = 288/298 (96%), Positives = 294/298 (98%) Query: 1 MADFDWVFFAEVLTGGLLSGVMYSLVAIGFVLIYKTSGVLNFAQGAQLLFAALTFVSLVE 60 M+DFD VFFAEVLTGGLLSGVMY+LVAIGFVLIYKTSGVLNFAQGAQLLFAALTFVSLVE Sbjct: 1 MSDFDGVFFAEVLTGGLLSGVMYALVAIGFVLIYKTSGVLNFAQGAQLLFAALTFVSLVE 60 Query: 61 RGVPFALALLLTLAAMILLGLSIERVVLRPLQNQSPMTLFMATLGLSYVIEGAAQLLWGT 120 RG+PF LALLLTLAAMILLGL+IERVVLRPLQNQSPM+LFMATLGLSYVIEGAAQLLWGT Sbjct: 61 RGIPFVLALLLTLAAMILLGLAIERVVLRPLQNQSPMSLFMATLGLSYVIEGAAQLLWGT 120 Query: 121 QVHALELGIADTPLQLGGMMISSFDLFAAATAAVMVALLSAFFRYTRLGLEFRALADDGF 180 QVHALELGIADTPLQLGGMMISSFDLFAAATAA+MVALLS FFRYTRLGLEFRALADDGF Sbjct: 121 QVHALELGIADTPLQLGGMMISSFDLFAAATAALMVALLSLFFRYTRLGLEFRALADDGF 180 Query: 181 AALAVGLRLPRILAAVWATAGLIALIAGLLWGARLGVQFSLSLVVLKALPVLVLGGFDSV 240 AALAVGLRLPRILAAVWATAGLIALIAGLLWGARLGVQFSLSLVVLKALPVLVLGGFDSV Sbjct: 181 AALAVGLRLPRILAAVWATAGLIALIAGLLWGARLGVQFSLSLVVLKALPVLVLGGFDSV 240 Query: 241 LGAIVGGLLIGALEKLAEVYLGPLVGGGIEGWFAYVAALAFLLVRPSGLFGQRAWQRA 298 LGAIVGGLLIGALEKLAEVYLGPLVG GIEGWFAYVAALAFLLVRPSGLFGQRAWQRA Sbjct: 241 LGAIVGGLLIGALEKLAEVYLGPLVGSGIEGWFAYVAALAFLLVRPSGLFGQRAWQRA 298 >gi|300311880|ref|YP_003775972.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 304 Score = 307 bits (786), Expect = 8e-85, Method: Compositional matrix adjust. Identities = 173/301 (57%), Positives = 218/301 (72%), Gaps = 11/301 (3%) Query: 8 FFAEVLTGGLLSGVMYSLVAIGFVLIYKTSGVLNFAQGAQLLFAALTFVSLVERGVPFAL 67 FF EV GLLSG+MYSLVA+GFVLIYK SGV NFAQGA + FAAL V L+E+G+P Sbjct: 3 FFLEVTLSGLLSGLMYSLVALGFVLIYKASGVFNFAQGAMVYFAALAVVGLMEKGMPMWA 62 Query: 68 ALLLTLAAMILLGLSIERVVLRPLQNQSPMTLFMATLGLSYVIEGAAQLLWGTQVHALEL 127 A++ MIL+GL+ ER VLR L NQ P+TLFMAT+GL++ +EG LL+G++V ++L Sbjct: 63 AIIGAFVVMILVGLATERFVLRKLVNQPPITLFMATIGLTFFLEGLGPLLFGSEVRPIDL 122 Query: 128 GIADTPLQLG----GMMISSFDLFAAATAAVMVALLSAFFRYTRLGLEFRALADDGFAAL 183 GI D P+Q G+ IS FDLFA+ +VA L+ FF+ T++G RA+ADD AAL Sbjct: 123 GIVDEPIQSVMDSIGIGISKFDLFASGMVVALVAALALFFQKTKVGRALRAVADDHQAAL 182 Query: 184 AVGLRLPRILAAVWATAGLIALIAGLLWGARLGVQFSLSLVVLKALPVLVLGGFDSVLGA 243 ++G+ L I A VW AG +ALIAGLLWG+R GVQF+L++ LKALPVL+LGGF S+ GA Sbjct: 183 SLGIPLQHIWAVVWGVAGFVALIAGLLWGSRNGVQFALTMTALKALPVLILGGFTSIPGA 242 Query: 244 IVGGLLIGALEKLAEVYLGPLVG-------GGIEGWFAYVAALAFLLVRPSGLFGQRAWQ 296 IVGGL+IGA EKLAE+Y+ P++ GGIEGWF YV AL FLLVRP GLFG+R Sbjct: 243 IVGGLIIGASEKLAEIYIPPVMQDAFGGNFGGIEGWFPYVFALLFLLVRPEGLFGERHID 302 Query: 297 R 297 R Sbjct: 303 R 303 >gi|300310521|ref|YP_003774613.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 309 Score = 120 bits (300), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 90/307 (29%), Positives = 159/307 (51%), Gaps = 16/307 (5%) Query: 8 FFAEVLTGGLLSGVMYSLVAIGFVLIYKTSGVLNFAQGAQLLFAALTFVSL------VER 61 F + + GL+ G MY+L+A+G+ ++Y ++NFA G L+ A+ +SL V Sbjct: 3 IFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQVAP 62 Query: 62 GVP----FALALLLTLAAMILLGLSIERVVLRPLQNQSPMTLFMATLGLSYVIEGAAQLL 117 G+P +A++ + I++ L IER+ RPL+N + + +G+S +++ A ++ Sbjct: 63 GLPGIVQLVIAIVGAIPVCIVVSLLIERIAYRPLRNAPRLAPLITAIGVSILLQTLAMMI 122 Query: 118 WGTQVHALELGIADTPLQLGGMMISSFDLFAAATAAVMVALLSAFFRYTRLGLEFRALAD 177 WG + P+ + G +IS + A A + + L T++G RA A+ Sbjct: 123 WGRSPLPFPQVMPSDPVHIAGALISPTQIMLLALAVLAMVGLVLIVEKTKMGRAMRATAE 182 Query: 178 DGFAALAVGLRLPRILAAVWATAGLIALIAGLLWGARLGV-QFSLSLVV-LKALPVLVLG 235 + A +G+ +++ +A +A IAG++W A QF++ V LKA VLG Sbjct: 183 NPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAVLG 242 Query: 236 GFDSVLGAIVGGLLIGALEKLAEVYLGPLVGG----GIEGWFAYVAALAFLLVRPSGLFG 291 G ++ GA++GG+L+G +E L Y+G L G + FA++ + L +RPSG+ G Sbjct: 243 GIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVLTLRPSGIMG 302 Query: 292 QRAWQRA 298 +R RA Sbjct: 303 ERVADRA 309 >gi|300309519|ref|YP_003773611.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 309 Score = 120 bits (300), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 90/307 (29%), Positives = 159/307 (51%), Gaps = 16/307 (5%) Query: 8 FFAEVLTGGLLSGVMYSLVAIGFVLIYKTSGVLNFAQGAQLLFAALTFVSL------VER 61 F + + GL+ G MY+L+A+G+ ++Y ++NFA G L+ A+ +SL V Sbjct: 3 IFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQVAP 62 Query: 62 GVP----FALALLLTLAAMILLGLSIERVVLRPLQNQSPMTLFMATLGLSYVIEGAAQLL 117 G+P +A++ + I++ L IER+ RPL+N + + +G+S +++ A ++ Sbjct: 63 GLPGIVQLVIAIVGAIPVCIVVSLLIERIAYRPLRNAPRLAPLITAIGVSILLQTLAMMI 122 Query: 118 WGTQVHALELGIADTPLQLGGMMISSFDLFAAATAAVMVALLSAFFRYTRLGLEFRALAD 177 WG + P+ + G +IS + A A + + L T++G RA A+ Sbjct: 123 WGRSPLPFPQVMPSDPVHIAGALISPTQIMLLALAVLAMVGLVLIVEKTKMGRAMRATAE 182 Query: 178 DGFAALAVGLRLPRILAAVWATAGLIALIAGLLWGARLGV-QFSLSLVV-LKALPVLVLG 235 + A +G+ +++ +A +A IAG++W A QF++ V LKA VLG Sbjct: 183 NPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAVLG 242 Query: 236 GFDSVLGAIVGGLLIGALEKLAEVYLGPLVGG----GIEGWFAYVAALAFLLVRPSGLFG 291 G ++ GA++GG+L+G +E L Y+G L G + FA++ + L +RPSG+ G Sbjct: 243 GIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVLTLRPSGIMG 302 Query: 292 QRAWQRA 298 +R RA Sbjct: 303 ERVADRA 309 >gi|300311114|ref|YP_003775206.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 296 Score = 93.6 bits (231), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 86/294 (29%), Positives = 145/294 (49%), Gaps = 21/294 (7%) Query: 16 GLLSGVMYSLVAIGFVLIYKTSGVLNFAQGAQLLFAALTFVSLVE--RGVPFA---LALL 70 G+ G++Y+++A G+ L + TSG LNF QG L+ AL +S+V G P+ L + Sbjct: 11 GIALGMIYAVIAFGYQLTFATSGTLNFGQGEALMLGALVGLSVVGNIHGGPYLNYWLMIP 70 Query: 71 LTLAAMILLGLSIERVVLRP-LQNQSPMTLFMATLGLSYVIEGAAQLLWGTQVHALELGI 129 + L L G+ +E + +RP ++ +S M+T+ L+ + + A+ +WG I Sbjct: 71 IVLVFGALQGMFVEWIGVRPAIKIKSEFGWIMSTIALAIIFKNVAENIWGKDDLPFPSPI 130 Query: 130 ADTPLQLGGMMISSFDLFAAATAAVMVALLSAFFRYTRLGLEFRALADDGFAALAVGLRL 189 + P Q+ G + + A +++A + F R + G A ++D AA +G+ Sbjct: 131 SGAPFQIFGANVQPMQVLVVVGALLIMAAVELFNRKSIYGKAVVATSNDRDAAGLMGINT 190 Query: 190 PRILAAVWATAGLIALIAGL------LWGARLGVQFSLSLVVLKALPVLVLGGFDSVLGA 243 ++ +A + A AG+ L GA +G LKA V ++GG S +GA Sbjct: 191 SMVITFSYALSSATAAFAGVLVAPLTLTGATMGAALG-----LKAFAVAIIGGLTSGMGA 245 Query: 244 IVGGLLIGALEKLAEVYLGPLVGGGIEGWFAYVAALAFLLVRPSGLFGQRAWQR 297 IVGGL++G E L Y+ G + V L L V+P+GLFG+ A ++ Sbjct: 246 IVGGLILGVAETLTGFYIST----GYKEVPGLVLLLLVLAVKPAGLFGKTAIKK 295 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.144 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 602,915 Number of extensions: 16493 Number of successful extensions: 176 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 157 Number of HSP's successfully gapped: 20 Length of query: 298 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 213 Effective length of database: 1,217,395 Effective search space: 259305135 Effective search space used: 259305135 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)