BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0113.p (386 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-... 729 0.0 gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [... 137 2e-33 gi|300310978|ref|YP_003775070.1| alkanesulfonate monooxygenase [... 107 2e-24 gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-... 76 4e-15 gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirill... 61 1e-10 >gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 729 bits (1881), Expect = 0.0, Method: Compositional matrix adjust. Identities = 371/386 (96%), Positives = 376/386 (97%), Gaps = 1/386 (0%) Query: 1 MSQAPATTTSATPRTDAVKFAYWVPNVSGGLVVSTIPQRTDWSLEYNQRLAVAAEKAGFE 60 MSQAP+TT +AT RTDAVKFAYWVPNVSGGLVVSTIPQRTDWSLEYNQRLAV AEKAGFE Sbjct: 1 MSQAPSTTAAAT-RTDAVKFAYWVPNVSGGLVVSTIPQRTDWSLEYNQRLAVTAEKAGFE 59 Query: 61 YALSQIRFTAGYGAEYQHESVSFSQALLHATTKLKVLAAILPGPWHPAVVAKQIATIDHI 120 YALSQIRFTAGYGAEYQHESVSFSQALLHATTKLKVLAAILPGPWHPAVVAKQIATIDHI Sbjct: 60 YALSQIRFTAGYGAEYQHESVSFSQALLHATTKLKVLAAILPGPWHPAVVAKQIATIDHI 119 Query: 121 SQGRIAINVVSGWFKGEFQAIGEPWLEHDERYRRSNEFIRALKGIWTQDDFTFKGDFYRF 180 S GRIAINVVSGWFKGEFQAIGEPWLEHDERYRRSNEFIRALKGIWTQDDFTFKGDFYRF Sbjct: 120 SNGRIAINVVSGWFKGEFQAIGEPWLEHDERYRRSNEFIRALKGIWTQDDFTFKGDFYRF 179 Query: 181 NNYTLSPKPVQKPHPEIFQGGSSRAARDNAASVSDWYFTNGNTVEGLKAQIDDIQAKAAQ 240 NNYTLSPKPVQKPHPEIFQGGSSRAARDNAASVSDWYFTNGNT+EGLKAQIDDIQAKAAQ Sbjct: 180 NNYTLSPKPVQKPHPEIFQGGSSRAARDNAASVSDWYFTNGNTLEGLKAQIDDIQAKAAQ 239 Query: 241 NGHKVRIGVNAFVIARDTEEEAQAVLQDIIDHAHVEAVHAFGDAVKQAGKASPEGEGNWA 300 NGHKVRIGVNAFVIARDTEEEAQAVLQDIIDHAHVEAVHAFGDAVKQAGKASPEGEGNWA Sbjct: 240 NGHKVRIGVNAFVIARDTEEEAQAVLQDIIDHAHVEAVHAFGDAVKQAGKASPEGEGNWA 299 Query: 301 KSTFDDLVQYNDGFKTNLIGTPQQIAERIVALKAIGVDLVLTGFLHFIEEVEYFGQKVLP 360 KSTF+DLVQYNDGFKTNLIGTPQQIAERIVALKAIGVDLVLTGFLHFIEEVEYFGQKVLP Sbjct: 300 KSTFEDLVQYNDGFKTNLIGTPQQIAERIVALKAIGVDLVLTGFLHFIEEVEYFGQKVLP 359 Query: 361 LVRELEARQATAAAAPQQAQALEAVA 386 LVRELEARQA AA+P A LEAVA Sbjct: 360 LVRELEARQAATAASPAAAPVLEAVA 385 >gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 375 Score = 137 bits (344), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 104/331 (31%), Positives = 167/331 (50%), Gaps = 20/331 (6%) Query: 43 SLEYNQRLAVAAEKAGFEYALSQIRFTAGYGAEYQHESVSFSQALLHATTKLKVLAAILP 102 +LEY +++AV +E G++ L GA V + +L+ T ++K+L A+ Sbjct: 28 TLEYMRQIAVTSETLGYDGLLIPT------GASCLDPWV-VAASLVPVTQRIKLLVALRT 80 Query: 103 GPWHPAVVAKQIATIDHISQGRIAINVVSGWFKGEFQAIGEPWLEHDERYRRSNEFIRAL 162 P A+Q AT+D GR+ +NVV G E +A G +L+HDERY ++E++ Sbjct: 81 SLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADGV-FLKHDERYEAADEYLTIW 139 Query: 163 KGIWTQDDFTFKGDFYRFNNYTLSPKPVQKPHPEIFQGGSSRAARDNAASVSDWYFTNGN 222 + + + ++G +R N L VQ+P+P ++ GGSS AA D AA D Y T G Sbjct: 140 RKLLQGETVNYEGKHFRIRNGQLFHPAVQQPYPPLYFGGSSPAAHDLAAKHVDAYLTWGE 199 Query: 223 TVEGLKAQIDDIQAKAAQNGHKVRIGVNAFVIARDTEEEAQAVLQDII------DHAHVE 276 ++ +IDD++ +AA+ G VR GV I R+T EA + +I D A + Sbjct: 200 PPAAVRQKIDDVRERAARQGRTVRFGVRLHAIVRETTAEAWTAAERLISKLTDEDIARAQ 259 Query: 277 AVHAFGDAVKQAGKASPEG----EGNWAKSTFDDLVQYNDGFKTNLIGTPQQIAERIVAL 332 +A D+V Q A+ G + A + + + G T+L+G +AER+ Sbjct: 260 QNYARMDSVGQQRMAALHGGRRDKLEIAPNLWAGVGLVRGGAGTSLVGDATTVAERLQEY 319 Query: 333 KAIGVD-LVLTGFLHFIEEVEYFGQKVLPLV 362 +GVD VL+G+ H +EE F + V PL+ Sbjct: 320 ADLGVDTFVLSGYPH-LEEAIRFAELVFPLL 349 >gi|300310978|ref|YP_003775070.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 107 bits (267), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 105/343 (30%), Positives = 164/343 (47%), Gaps = 40/343 (11%) Query: 43 SLEYNQRLAVAAEKAGFEYALSQIRFTAGYGAEYQHESVSFSQALLHATTKLKVLAAILP 102 S +Y +++AVAA+ G++ L G E ++ + +L+ AT +LK L A+ P Sbjct: 26 SHDYMKQVAVAADTLGYDGVL----LPTGRSCE---DAWVAAASLIDATRRLKFLVAVRP 78 Query: 103 GPWHPAVVAKQIATIDHISQGRIAINVVSGWFKGEFQAIGEPWLEHDERYRRSNEFIRAL 162 G P + A+ AT D +S GR+ INVV+G E ++ G + H ERY S+EF++ Sbjct: 79 GLMAPTLAARMAATFDRLSGGRLLINVVTGGDPVEQESDGV-FGSHAERYEISDEFLKIW 137 Query: 163 KGIWTQDDFTFKGDFYRFNNYTLSPKPVQ-------KPHPEIFQGGSSRAARDNAASVSD 215 + + T+ T G+ F LS K + +P+P ++ GGSS A D AA D Sbjct: 138 RDVLTK---TRSGEPTHFQGKHLSVKGAKALYPPVQQPYPPLYFGGSSDEAIDLAAEQVD 194 Query: 216 WYFTNGNTVEGLKAQIDDIQAKAAQNGHKVRIGVNAFVIARDTEEEAQAVLQDIIDHAHV 275 Y T G + +I ++ +AA+ G +R G+ VI R+T E A ++I Sbjct: 195 VYLTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRETNEAAWRAADELISRLDD 254 Query: 276 EAVHAFGDAVKQAGKASPEGEGNWAKSTF-----DDL-VQYN---------DGFKTNLIG 320 E + A QA A + EG + D L V N G T L+G Sbjct: 255 ETI-----AKAQANFARMDSEGQRRMAALHGGRRDKLEVSPNLWAGVGLVRGGAGTALVG 309 Query: 321 TPQQIAERIVALKAIGVD-LVLTGFLHFIEEVEYFGQKVLPLV 362 +AER+ A+G+D +L+G+ H +EE F + V PL+ Sbjct: 310 DGPTVAERLQEYAALGIDTFILSGYPH-LEESYRFAELVFPLL 351 >gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 76.3 bits (186), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 70/307 (22%), Positives = 137/307 (44%), Gaps = 51/307 (16%) Query: 107 PAVVAKQIATIDHISQGRIAINVVSGWFKGEFQAIG-EPWLEHDERYRRSNEFIRALKGI 165 P + A++++T+DH+S+GR+ N+V+G+ +A+G LEHD+RY R++EF+ + + Sbjct: 113 PFLFARRMSTLDHLSRGRVGWNIVTGYLDSAARAMGLHEQLEHDQRYDRADEFMEVVYKL 172 Query: 166 WT---QDDFTFK-------------------GDFYRFNNYTLSPKPVQKPHPEIFQGGSS 203 W +D + GD+Y LS +P ++ P ++Q GSS Sbjct: 173 WEGSWEDGAVLRDRARRVYADPAKVHKIAHHGDYYHVEGIHLS-EPSRQRTPVLYQAGSS 231 Query: 204 RAARDNAASVSDWYFTNGNTVEGLKAQIDDIQAKAAQNGHK---VRIGVNAFVIARDTEE 260 + A ++ F + + + + + I+ A + G ++I + V+ TE+ Sbjct: 232 GRGQAFGARHAECVFISSQSKDATRKLVQSIRDGAVREGRDPDAIKIFMGLTVVTGRTEQ 291 Query: 261 EAQAVLQDIIDHAHVEA--VH--------------------AFGDAVKQAGKASPEGEGN 298 +AQ ++ +A EA H A +A++ K + E N Sbjct: 292 QAQERYREYARYASPEAGLAHYSAGTGIDFSQYELDEPIRPAKTNAIESLTKVVTQPELN 351 Query: 299 WAKSTFDDLVQYNDGFKTNLIGTPQQIAERIVALKA-IGVDLVLTGFLHFIEEVEYFGQK 357 W K ++ G +++G P ++A+ + + A G+D E E F + Sbjct: 352 WTKRKLLQQLELG-GRYASIVGAPDRVADELESWVADTGIDGYNLTRTVTPESFEDFVEL 410 Query: 358 VLPLVRE 364 V+P+++E Sbjct: 411 VIPVLQE 417 >gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirillum seropedicae SmR1] Length = 433 Score = 61.2 bits (147), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 70/270 (25%), Positives = 116/270 (42%), Gaps = 43/270 (15%) Query: 44 LEYNQRLAVAAEKAGFE--YALSQIRFTAGYGAEYQH------------ESVSFSQALLH 89 L+Y +A E+ F+ + ++ + YG + H + + AL Sbjct: 32 LDYYLHIARTLERGKFDLLFFADRLAVSTRYGDSHLHGLYIGDQDATRLDPLPILGALAA 91 Query: 90 ATTKLKVLAAILPGPWHPAVVAKQIATIDHISQGRIAINVVSGWFKGEFQAIG-EPWLEH 148 AT+ + + A P +A++ AT+DH+S GR A NVV+ +GE G + L H Sbjct: 92 ATSHIGLGATRSTTYSQPYSLAREFATLDHLSGGRAAWNVVTSVNQGEADNFGLKETLPH 151 Query: 149 DERYRRSNEFIRALKGIWTQ---DDFTFKGDFYRFNNYTLSPK----------PVQKP-- 193 D RY R++EF+ +W D + D Y + + P+ P Sbjct: 152 DVRYDRADEFLEVTHKLWHSWQPDALRLQQDRYADADKVAAIDHQGAHFSVRGPLNIPAS 211 Query: 194 ---HPEIFQGGSSRAARDNAASVSDWYFTNGNTVEGLKAQIDDIQAKAAQNGHK---VRI 247 HP I Q GSS +D AA ++ F + L+A D++ +A Q G + +RI Sbjct: 212 PQGHPVIIQAGSSDRGQDFAARWAEVVFNIQPDLPRLQAFYRDLKNRAQQFGRRPQDIRI 271 Query: 248 --GVNAFV-----IARDTEEEAQAVLQDII 270 V FV AR+ +E+A A+ ++ Sbjct: 272 LSAVMPFVGQSRQHARELQEQANALADPLV 301 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,114,954 Number of extensions: 46184 Number of successful extensions: 143 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 138 Number of HSP's successfully gapped: 5 Length of query: 386 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 299 Effective length of database: 1,207,925 Effective search space: 361169575 Effective search space used: 361169575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)