BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0109.p (505 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311699|ref|YP_003775791.1| cation transport P-type ATPase ... 622 e-179 gi|300312503|ref|YP_003776595.1| cation transport P-type ATPase ... 164 2e-41 gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPas... 145 8e-36 gi|300310937|ref|YP_003775029.1| heavy metal translocating P-typ... 134 2e-32 gi|300313072|ref|YP_003777164.1| high-affinity K+ transport syst... 89 1e-18 >gi|300311699|ref|YP_003775791.1| cation transport P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 734 Score = 622 bits (1603), Expect = e-179, Method: Compositional matrix adjust. Identities = 342/505 (67%), Positives = 407/505 (80%), Gaps = 3/505 (0%) Query: 1 VVATPCPLILAVPVALVCAMSRCAQQGILIKHGGALEKLAQLHTLFVDKTGTLTTGKAHL 60 VVATPCPLILAVPVA+VCAMSRCAQ+G+LIKHGGALEKLAQ+ TLF DKTGTLT GKA L Sbjct: 233 VVATPCPLILAVPVAIVCAMSRCAQRGVLIKHGGALEKLAQVRTLFFDKTGTLTAGKARL 292 Query: 61 MALHPAPGIGADELLRLSASLEQMSVHAIAQAVVSAALERGLDLALPEQVRESPGAGLSG 120 M + P I ADELLRL+ASLEQMS H IAQAVVSAA+ER L L++P+QV+E PGAGLSG Sbjct: 293 MNMVTTPSIQADELLRLAASLEQMSNHVIAQAVVSAAIERALVLSIPDQVQEQPGAGLSG 352 Query: 121 RVEGRALRVGTLDHVLAGAALPAWAVPLNQRIGYEGVAGVYVAIEGRFAGVLLLADEVRL 180 RV+G+ +RVG + +V+ +A P W L R+ YEG AGV VA++G+ GVL LAD++RL Sbjct: 353 RVDGKLVRVGAMQYVIGPSAAPPWTSGLRDRLKYEGGAGVCVAVDGQLCGVLYLADDIRL 412 Query: 181 EAPRALRLLRTLGAQRIVMLTGDRHDVATTIGRALGVDEILPEQSPAAKLAAITAARQQG 240 + PRALR LR LGA+RIVMLTGDR DVA TIG +LG D+++PEQSPA+KLAAI A ++G Sbjct: 413 DTPRALRQLRALGAKRIVMLTGDRRDVAMTIGSSLGFDDVMPEQSPASKLAAIKQASREG 472 Query: 241 VTLMVGDGVNDAPALAAADIGVAMGARGAAAAAESAQVVLLVDRLDRLVFAVQLAQQTRR 300 VT+MVGDG+NDAPALAAADIGVAMGARGAAAAAESAQVVLLVDRLDRL FAV++AQQ R+ Sbjct: 473 VTMMVGDGINDAPALAAADIGVAMGARGAAAAAESAQVVLLVDRLDRLAFAVRMAQQARK 532 Query: 301 IALQSVMAGMGLSVLAMLVAAFGYLPPVAGAVLQEGIDVAVILNALRVLTLQKDVLRSRL 360 IALQSV+AGMGLS AML AAFGYLPPVAGA+LQE IDVAVILNALRVL +++ L RL Sbjct: 533 IALQSVVAGMGLSGCAMLAAAFGYLPPVAGAILQEFIDVAVILNALRVLYVERGNLSDRL 592 Query: 361 PAGERERLQQEHAALVPILDRLLWLAGHSDVLTVSALREALQSLNGLLKTRLLPHETEDD 420 A + + L++EH L PI+DRL+WLA + L+ +A+RE L L+ L+ LL HE +DD Sbjct: 593 SAEDCQTLREEHVRLQPIIDRLVWLADRVEDLSSAAMREELVLLSAQLQGNLLQHEAQDD 652 Query: 421 ARLYPRLATLIGGRDPMAAMSSTHREIHRLSRTIERIAVQLGTSPADPSLAPATAQELRH 480 LYPRLA LIGG DPM AMSS HREI+RLSRT++R+A Q +P D A E+R Sbjct: 653 GVLYPRLAQLIGGEDPMGAMSSMHREIYRLSRTVDRLATQ---APDDEQQDDAFMLEVRR 709 Query: 481 ALYALEAILRLHIAQEEELFHGLSS 505 LYAL A+LRLH AQEEE+FHGLS+ Sbjct: 710 TLYALHAVLRLHFAQEEEIFHGLSA 734 >gi|300312503|ref|YP_003776595.1| cation transport P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 839 Score = 164 bits (414), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 126/351 (35%), Positives = 183/351 (52%), Gaps = 11/351 (3%) Query: 1 VVATPCPLILAVPVALVCAMSRCAQQGILIKHGGALEKLAQLHTLFVDKTGTLTTGKAHL 60 VV+ PC L LA P AL A R + G LI G LE L + T+ DKTGTLT G+ L Sbjct: 473 VVSCPCALSLATPSALAAATDRLLRAGTLIVRGNVLETLQRADTIVFDKTGTLTQGRPQL 532 Query: 61 MALHPAPGIGADELLRLSASLEQMSVHAIAQAVVSAALERGLDLALPEQVRESPGAGLSG 120 AL + L ++A++E+ S+H +A+A+V R +D EQ+ GAG+ G Sbjct: 533 TALW--SDVDCQRSLAIAAAMERGSLHPLAKALVEEGARRQVDALEVEQLTSVTGAGMQG 590 Query: 121 RVEGRALRVGTLDHV--LAGAALPAWAVPLNQRIGYEGVAGVYVAIEGRFAGVLLLADEV 178 +EG R+G+ V LAG ALP P+ + G VY+ + LAD + Sbjct: 591 MIEGDLYRIGSRSFVAGLAGTALP----PVLLQSAASGAGSVYLGSSDGWLARFDLADAL 646 Query: 179 RLEAPRALRLLRTLGAQRIVMLTGDRHDVATTIGRALGVDEILPEQSPAAKLAAITAARQ 238 R +A + R LG R ++L+GD+ +V + A G+ E+ +P KL + ++ Sbjct: 647 RTDAVATVAAFRALGL-RTILLSGDQPEVCAEVAAAAGISEVQAACTPQGKLDYVRNLQR 705 Query: 239 QG-VTLMVGDGVNDAPALAAADIGVAMGARGAAAAAESAQVVLLVDRLDRLVFAVQLAQQ 297 G V +VGDG+NDA L A D+ AMG +GAA A SA V++ DRL + +AQ+ Sbjct: 706 DGAVVAIVGDGINDAAMLRAGDVSFAMG-KGAALAQVSADAVIMSDRLQAVADCAAMAQR 764 Query: 298 TRRIALQSVMAGMGLSVLAMLVAAFGYLPPVAGAVLQEGIDVAVILNALRV 348 T I Q+++ + LA+ AAFG L P AV + V+ NALR+ Sbjct: 765 TMHIVRQNLVWAATYNFLAIPAAAFGLLDPWMSAVGMSLSSLLVVGNALRL 815 >gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 778 Score = 145 bits (366), Expect = 8e-36, Method: Compositional matrix adjust. Identities = 123/360 (34%), Positives = 191/360 (53%), Gaps = 22/360 (6%) Query: 1 VVATPCPLILAVPVALVCAMSRCAQQGILIKHGGALEKLAQLHTLFVDKTGTLTTGK--- 57 V+A PC L+++ PV +V ++ A+ GIL+K G LE+ QL L +DKTGTLT GK Sbjct: 420 VIACPCALVISTPVTIVSGLASAARHGILVKGGVYLEQGRQLRALALDKTGTLTFGKPRV 479 Query: 58 AHLMALHPAPGIGADE--LLRLSASLEQMSVHAIAQAVVSAALERGLDLALPEQVRESPG 115 +++L AD+ LL+L+ SL S H +++A+ AA E + + E G Sbjct: 480 TDVVSLQAQ----ADQQALLQLAVSLAARSDHPVSRAI--AAQEEIATVEVSE-FAALAG 532 Query: 116 AGLSGRVEGRALRVGT--LDHVLAGAALPAWAVPLNQRIGYEGVAGVYVAIEGRFAGVLL 173 G+ GR+ G+ L +G L H L G P L Q +G + ++G+ ++ Sbjct: 533 RGVQGRINGQLLHLGNHRLVHEL-GQCSPELEARL-QAFEKQGKTTTVLCLDGQPQLLVA 590 Query: 174 LADEVRLEAPRALRLLRTLGAQRIVMLTGDRHDVATTIGRALGVDEILPEQSPAAKLAAI 233 +AD VR + +A+ LR LG + ++MLTGD A I G+ + +Q P KL AI Sbjct: 591 VADTVRPSSRQAVAELRALGVE-VIMLTGDNSHTAQAIAAQTGISDARGDQLPEDKLKAI 649 Query: 234 TAARQQ----GVTLMVGDGVNDAPALAAADIGVAMGARGAAAAAESAQVVLLVDRLDRLV 289 Q+ GV MVGDG+NDAPALA ADIG AMGA G A E+A V L+ D L ++ Sbjct: 650 EELSQRHGVRGVG-MVGDGINDAPALARADIGFAMGAAGTDTALETADVALMDDDLRKIP 708 Query: 290 FAVQLAQQTRRIALQSVMAGMGLSVLAMLVAAFGYLPPVAGAVLQEGIDVAVILNALRVL 349 ++L+++ + +Q++ +G+ + + +A G G + V+ N LR++ Sbjct: 709 DFIRLSRKAHAVLVQNISIALGIKAVFLGLALVGMSSLWMAVFADMGASLIVVFNGLRLV 768 >gi|300310937|ref|YP_003775029.1| heavy metal translocating P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 134 bits (337), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 129/358 (36%), Positives = 185/358 (51%), Gaps = 20/358 (5%) Query: 1 VVATPCPLILAVPVALVCAMSRCAQQGILIKHGGALEKLAQLHTLFVDKTGTLTTGKAHL 60 V+A PC L LA P A++ A+ GILIK ALE +++ + DKTGTLT G L Sbjct: 380 VIACPCALGLATPTAIMAGTGVAARHGILIKDAEALEIAHRVNAVAFDKTGTLTLGHPRL 439 Query: 61 MALHPAPGIGADELLRLSASLEQMSVHAIAQAVVSAALERGLDLALPEQVRESPGAGLSG 120 + L P DELL L+A+L+ S H + +AV AA + EQVR G G+ G Sbjct: 440 LELEATPSTQPDELLALAAALQAHSEHPLGRAVREAAAQSAQAARQAEQVRSVAGLGIEG 499 Query: 121 RVEGRALRVGT---LDHVLAGA-ALPAWAVPLNQR------IGYEGVAGVYVAIEGRFAG 170 VEGR L +G+ + + A A AL A A L + + +GV V G Sbjct: 500 VVEGRQLMLGSSRWMQQLQAHAPALQARAEQLQAQGRTLSWLARRNDSGVEV------LG 553 Query: 171 VLLLADEVRLEAPRALRLLRTLGAQRIVMLTGDRHDVATTIGRALGVDEILPEQSPAAKL 230 +L D+++ A A+ L +G R ML+GD A + LG+ E+ E PA K Sbjct: 554 LLAFGDQIKPGAQAAIATLHRMGV-RSAMLSGDNLGAAQQVASELGIQEVRAEVLPADKA 612 Query: 231 AAITAARQQG--VTLMVGDGVNDAPALAAADIGVAMGARGAAAAAESAQVVLLVDRLDRL 288 A+TA RQ G V MVGDGVNDAPALAAAD+G+AM + G A ++A V L+ + Sbjct: 613 EAVTALRQGGAQVVAMVGDGVNDAPALAAADVGMAM-STGTDVAMQAAGVTLMHGDPGLV 671 Query: 289 VFAVQLAQQTRRIALQSVMAGMGLSVLAMLVAAFGYLPPVAGAVLQEGIDVAVILNAL 346 A+ ++++T Q++ +++ + +AA G L P+ V+V+ NAL Sbjct: 672 ADAIDISRRTYAKIRQNLFWAFIYNLVGIPLAALGLLNPMLAGAAMAFSSVSVVSNAL 729 >gi|300313072|ref|YP_003777164.1| high-affinity K+ transport system ATPase [Herbaspirillum seropedicae SmR1] Length = 730 Score = 88.6 bits (218), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 119/376 (31%), Positives = 166/376 (44%), Gaps = 49/376 (13%) Query: 17 VCAMSRCAQQGILIKHGGALEKLAQLHTLFVDKTGTLTTGKAHLMALHPAPGIGADELLR 76 V MSR Q ++ G A+E + L +DKTGT+T G A PAPG+ EL Sbjct: 312 VAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQAAAFMPAPGVTERELAD 371 Query: 77 LS--ASLEQMSVHAIAQAVVSAALERGLDLALPEQVRESPGAGL-----SGRVEG----- 124 ++ ASL + +++V A R L E+ + GA RV G Sbjct: 372 VAQLASLADETPE--GRSIVVLAKNR---FNLREREMANLGAEFIPFSAQTRVSGIDLGH 426 Query: 125 RALRVGTLDHV--LAGAA---LPAWAVPLNQRIGYEGVAGVYVAIEG------------- 166 R +R G D + L AA LPA + I G V IE Sbjct: 427 RRIRKGAADAIRKLTRAADQRLPAQVESDIESIARRG-GTPLVVIEWHAEAAMINQGAPI 485 Query: 167 RFAGVLLLADEVRLEAPRALRLLRTLGAQRIVMLTGDRHDVATTIGRALGVDEILPEQSP 226 R G + L D V+ LR +G + VM+TGD A I GVD+ L E +P Sbjct: 486 RILGTVELKDIVKGGIKERFGELRRMGI-KTVMITGDNRLTAAAIAAEAGVDDFLAEATP 544 Query: 227 AAKLAAITAARQQG-VTLMVGDGVNDAPALAAADIGVAMGARGAAAAAESAQVVLLVDRL 285 KLA I + +G + M GDG NDAPALA AD+ VAM + G AA E+ +V L Sbjct: 545 EQKLALIRQHQAEGKLVAMTGDGTNDAPALAQADVAVAMNS-GTQAAKEAGNMVDLDSNP 603 Query: 286 DRLVFAVQLAQQ---TR----RIALQSVMAGMGLSVLAMLVAAF---GYLPPVAGAVLQE 335 +L+ V++ +Q TR ++ + +A + AM V ++ G L + A Sbjct: 604 TKLIEIVEIGKQMLMTRGSLTTFSIANDVAKYFAIIPAMFVGSYPQLGALNVMHLASPDS 663 Query: 336 GIDVAVILNALRVLTL 351 I AVI NAL ++ L Sbjct: 664 AIMSAVIFNALVIVAL 679 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,507,631 Number of extensions: 62902 Number of successful extensions: 371 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 358 Number of HSP's successfully gapped: 6 Length of query: 505 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 416 Effective length of database: 1,198,455 Effective search space: 498557280 Effective search space used: 498557280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)