BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0105.p (504 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Her... 196 5e-51 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 184 1e-47 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 178 9e-46 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 178 1e-45 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 172 6e-44 >gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 487 Score = 196 bits (497), Expect = 5e-51, Method: Compositional matrix adjust. Identities = 142/469 (30%), Positives = 231/469 (49%), Gaps = 23/469 (4%) Query: 24 IRSPRDGEVIAQLKAQTPADAEAAIAGAQQAFLAWREVPAPVRGELVRVLGEVLREHRDE 83 + P G + Q+ +P D A++ A A AW +P R ++ R + +H DE Sbjct: 30 VLEPATGRPLTQVGLASPDDVSASVELAIAAQAAWVAMPPRERADIFRRAAGLFHQHFDE 89 Query: 84 LGALVTQEAGKILSEGLGEVQEMIDICDFAVGLSRQLHGLTIASERPGHRMMETWHPLGV 143 L +V +E G +L +G EV+E I +C A G+ Q G + S PG + PLGV Sbjct: 90 LARMVARETGGVLFKGEHEVREAITLCHLAAGMPLQAQGQLLPST-PGRLSIARRAPLGV 148 Query: 144 VGVITAFNFPVAVWAWNAALALVCGNAVVWKPSEKTPVTA-LAVQALFAKAVARFSAQRP 202 +GVI+ FNFP+ + A AL GNAVV KP +TPV+ + +F +A Sbjct: 149 IGVISPFNFPLILTLRTVAPALAAGNAVVVKPDLRTPVSGGFMLARIFEQAG-------- 200 Query: 203 NLVPAQLCGLLIGGADLGETLSRSATVPLVSATGSVRMGRKVATVVAERLGRSLLELGGN 262 +PA L +L GGA+ GE L + VP+++ TGS +GR++ + L + LELGG Sbjct: 201 --LPAGLLHVLPGGAETGEALVTAPGVPMIAFTGSPAVGRRIGELAGRHLKKLSLELGGA 258 Query: 263 NAMIVAPSADLNLALRAITFSAVGTAGQRCTSLRRLFVHSSIHDQVVAQIKRIYASVKVG 322 NA+++ ADL++A + A GQ C + R+ VH+S+ + ++ ++ + VG Sbjct: 259 NALVILEDADLDVAASHAAWGAWLHQGQICMAANRILVHASLEEGLLQRLADKARHLPVG 318 Query: 323 DPLQGDTLVGPLIDQAAFDAMQAALKQARAQGGEVTGGERVDVMGQQSWYVRPALI--TM 380 D G +GP+ID+ + A ++++ A G ++ G R + + + +P ++ Sbjct: 319 DGASGQVALGPMIDERQLQRVHALVQESVAAGAQLVAGGRYEQL-----FYQPTVLGGVR 373 Query: 381 PGQTAIMHHETFAPILYIVKYDELGDAIAMNNAVPQGLSSAIFTNDMREAELFVSAVGSD 440 PG + E F P+ ++++D DAIA+ N GL++ + + + A A Sbjct: 374 PGMR-VFEEEIFGPVASVIRFDNDEDAIALANRHAGGLAAGVLSPSVGRA--MAVAARLR 430 Query: 441 CGLANVNIGTSGAEIGGAFGGEKETGGGRESGSDA-WKNYMRRATNTIN 488 G+ +VN T E FGG G G G A W+ Y + TI Sbjct: 431 QGMVHVNDQTVNDECVNPFGGPGIAGNGASVGGPADWEEYTQWRWTTIR 479 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 184 bits (468), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 128/467 (27%), Positives = 233/467 (49%), Gaps = 21/467 (4%) Query: 18 AGNDLVIRSPRDGEVIAQLKAQTPADAEAAIAGAQQAFLAWREVPAPVRGELVRVLGEVL 77 +G + + +P EVI ++ AD + A+ A + F WR + A R +++R ++L Sbjct: 18 SGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFERSKIMRRAADLL 77 Query: 78 REHRDELGALVTQEAGKILSEGLGEVQEMIDICDFAVGLSRQLHGLTIASERPGHRMMET 137 RE +E+ L+T E GK L+E E D D+ +R+ +G + + PG M Sbjct: 78 RERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLVPARVPGAYQMVI 137 Query: 138 WHPLGVVGVITAFNFPVAVWAWNAALALVCGNAVVWKPSEKTPVTALAVQALFAKAVARF 197 P+G V T +NFP+ + AL G +++ K E+TP + + +A A Sbjct: 138 KEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELIRAYADAG--- 194 Query: 198 SAQRPNLVPAQLCGLLIG-GADLGETLSRSATVPLVSATGSVRMGRKVATVVAERLGRSL 256 VPA + L+ G A++ E L + +S TGS +G+++A + + + R+ Sbjct: 195 -------VPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMKRAT 247 Query: 257 LELGGNNAMIVAPSADLNLALRAITFSAVGTAGQRCTSLRRLFVHSSIHDQVVAQIKRIY 316 +ELGG+ +V AD+++A + + + AGQ C S R V + + V + Sbjct: 248 MELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFVTYA 307 Query: 317 ASVKVGDPLQGDTLVGPLIDQAAFDAMQAALKQARAQGGEV-TGGERVDVMGQQSWYVRP 375 +KVG+ L+ +GP+ ++ A++A + A +QG ++ TGG+R+ G + ++ +P Sbjct: 308 EQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRI---GNKGYFFQP 364 Query: 376 ALIT-MPGQTAIMHHETFAPILYIVKYDELGDAIAMNNAVPQGLSSAIFTNDMREAELFV 434 ++T +P Q M+ E F P+ I ++ L + I N +P GL++ F Sbjct: 365 TVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHALA 424 Query: 435 SAVGSDCGLANVN-IGTSGAEIGGAFGGEKETGGGRESGSDAWKNYM 480 + V + G+ +N +G + E+ FGG K++G G E G++A + Y+ Sbjct: 425 ARV--ESGMLTINHLGLALPEL--PFGGIKDSGYGSEGGTEAIEAYI 467 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 178 bits (451), Expect = 9e-46, Method: Compositional matrix adjust. Identities = 129/463 (27%), Positives = 221/463 (47%), Gaps = 15/463 (3%) Query: 19 GNDLVIRSPRDGEVIAQLKAQTPADAEAAIAGAQQAFLAWREVPAPVRGELVRVLGEVLR 78 G L + +P +GE++ + A+ AI A A+ AW++ A R ++R E++ Sbjct: 26 GERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKERSAILRRWYELML 85 Query: 79 EHRDELGALVTQEAGKILSEGLGEVQEMIDICDFAVGLSRQLHGLTIASERPGHRMMETW 138 + D+L ++T E GK L+E GE+ ++ ++ +G TI S P +R++ Sbjct: 86 ANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTIPSPSPSNRIVVIK 145 Query: 139 HPLGVVGVITAFNFPVAVWAWNAALALVCGNAVVWKPSEKTPVTALAVQALFAKAVARFS 198 +GV IT +NFP A+ A AL G +V KP+E TP +ALA+ L Sbjct: 146 EAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALALAVL--------- 196 Query: 199 AQRPNLVPAQLCGLLIGGAD-LGETLSRSATVPLVSATGSVRMGRKVATVVAERLGRSLL 257 A+R +PA + ++ G +G ++ + V +S TGS +G+ + A + + L Sbjct: 197 AERAG-IPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKKLSL 255 Query: 258 ELGGNNAMIVAPSADLNLALRAITFSAVGTAGQRCTSLRRLFVHSSIHDQVVAQIKRIYA 317 ELGGN IV ADL+ A+ S AGQ C R++V ++D A++ Sbjct: 256 ELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEAVK 315 Query: 318 SVKVGDPLQGDTLVGPLIDQAAFDAMQAALKQARAQGGEVTGGERVDVMGQQSWYVRPAL 377 KVG + GPLI++ A ++ + A A+G V G + +G S++ L Sbjct: 316 KFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALG-HSFFEPTVL 374 Query: 378 ITMPGQTAIMHHETFAPILYIVKYDELGDAIAMNNAVPQGLSSAIFTNDMREAELFVSAV 437 + + ETF P+ + ++ + +A+ N GL+S ++ D+ ++ A Sbjct: 375 ADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDI--GRIWRVAE 432 Query: 438 GSDCGLANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKNYM 480 G + G+ +N G E+ FGG K++G GRE +Y+ Sbjct: 433 GLESGMVGINTGLISNEV-APFGGVKQSGLGREGSHYGIDDYL 474 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 178 bits (451), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 158/487 (32%), Positives = 238/487 (48%), Gaps = 37/487 (7%) Query: 10 LGADFSAYA-GNDLVIRSPRDGEVIAQLKAQTPADAEAAIAGAQQAFLAWREVPAPVRGE 68 +GAD+ A G L + P G+V A + ADA AA+ A AF AWR PA R Sbjct: 18 IGADWRGAADGRQLDVSDPATGQVFASVPDGGAADARAAVEAAVAAFAAWRATPAKQRAG 77 Query: 69 LVRVLGEVLREHRDELGALVTQEAGKILSEGLGEVQEMIDICDFAVGLSRQLHGLTIASE 128 +++ ++L H+D+LG L+++E GK L+E GEV ++ + + +G I + Sbjct: 78 IIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRANGDIIPAP 137 Query: 129 RPGHRMMETWHPLGVVGVITAFNFPVAVWAWNAALALVCGNAVVWKPSEKTPVTALAVQA 188 G RMM P+GVV IT +NFP A+ A A AL G VV KP+E TP+T+LA+ Sbjct: 138 VTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTPLTSLALVR 197 Query: 189 LFAKAVARFSAQRPNLVPAQLCGLLIGGADLGETLSRSAT------------VPLVSATG 236 L AQ G+ +G ++ T SR T V +S TG Sbjct: 198 L-----------------AQEAGVPVGVINI-VTASRERTPEVVDVWLADGRVRKISFTG 239 Query: 237 SVRMGRKVATVVAERLGRSLLELGGNNAMIVAPSADLNLALRAITFSAVGTAGQRCTSLR 296 S +G+ +A A+ L + LELGGN IV AD++ A+ + + GQ C S Sbjct: 240 STAVGKHLARHSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPN 299 Query: 297 RLFVHSSIHDQVVAQIKRIYASVKVGDPLQGDTLVGPLIDQAAFDAMQAALKQARAQGGE 356 R++V ++D V ++ A++KVG + +GP+I+ A + ++ A A+G Sbjct: 300 RIYVQEKVYDAFVDKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIARGAR 359 Query: 357 V-TGGERVD--VMGQQSWYVRPALITMPGQTAIMHHETFAPILYIVKYDELGDAIAMNNA 413 V TGG+R+ G ++Y L + G ETF P+ I ++ + IA NA Sbjct: 360 VITGGKRLQGPGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVIAAANA 419 Query: 414 VPQGLSSAIFTNDMREAELFVSAVGSDCGLANVNIGTSGAEIGGAFGGEKETGGGRESGS 473 P GL++ ++ D+R A+ S G+ VN G AE FGG KE+G GRE Sbjct: 420 TPFGLAAYFYSTDVRRIHRVTDALES--GIVGVNEGALAAE-AAPFGGVKESGYGREGSV 476 Query: 474 DAWKNYM 480 +Y+ Sbjct: 477 HGLDDYL 483 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 172 bits (436), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 138/442 (31%), Positives = 210/442 (47%), Gaps = 21/442 (4%) Query: 24 IRSPRDGEVIAQLKAQTPADAEAAIAGAQQAFLAWREVPAPVRGELVRVLGEVLREHRDE 83 +R P G+V+ +L AD E AI A +AF WR+V R +++R + RE Sbjct: 24 VRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWRQVSPLERSDILRRAAALARERAQA 83 Query: 84 LGALVTQEAGKILSEGLGEVQEMIDICDFAVGLSRQLHGLTIASERPGHRMMETWHPLGV 143 + +T + GK L++ + E+ + D+ R+L+G + + P R + P+GV Sbjct: 84 MAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRRLYGRVVGARHPEVRQLVVREPVGV 143 Query: 144 VGVITAFNFPVAVWAWNAALALVCGNAVVWKPSEKTPVTALAVQALFAKAVARFSAQRPN 203 I+ +NFP A A+ G VV K ++P + + LF A Sbjct: 144 CAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESPAAIVLMAQLFHDAG--------- 194 Query: 204 LVPAQLCGLLIG-GADLGETLSRSATVPLVSATGSVRMGRKVATVVAERLGRSLLELGGN 262 VPA + ++ G +++ + L S V +S TGSV +G K+A A + R +ELGG+ Sbjct: 195 -VPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQAALHMKRMSMELGGH 253 Query: 263 NAMIVAPSADLNLALRAITFSAVGTAGQRCTSLRRLFVHSSIHDQVVAQIKRIYASVKVG 322 ++V A++ A R + AGQ C S R +V I+ + V +KVG Sbjct: 254 APVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARFVTAFAEALLQIKVG 313 Query: 323 DPLQGDTLVGPLIDQAAFDAMQAALKQARAQGGE-VTGGERVDVMGQQSWYVRPALIT-M 380 + +GPLI F+A+Q + A AQG E VTGG R +GQ+ + P LIT + Sbjct: 314 PGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSELVTGGAR---LGQRGHFYAPTLITGV 370 Query: 381 PGQTAIMHHETFAPILYIVKYDELGDAIAMNNAVPQGLSSAIFTNDMREAELFVSAVGSD 440 P +M E F PI + + + +AIAM NA+P GL+S +FT + A V D Sbjct: 371 PPSARVMQEEPFGPIAAVTPFSQAEEAIAMANALPLGLASYVFTQSLDTAH----RVSRD 426 Query: 441 CGLANVNIGTSG-AEIGGAFGG 461 VNI SG A FGG Sbjct: 427 LQAGMVNINHSGMAHPELPFGG 448 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,387,190 Number of extensions: 53295 Number of successful extensions: 282 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 235 Number of HSP's successfully gapped: 19 Length of query: 504 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 415 Effective length of database: 1,198,455 Effective search space: 497358825 Effective search space used: 497358825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)