BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0103.p (467 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312605|ref|YP_003776697.1| FAD/FMN-containing dehydrogenas... 238 6e-64 gi|300313123|ref|YP_003777215.1| D-lactate dehydrogenase [Herbas... 124 2e-29 gi|300313121|ref|YP_003777213.1| glycolate oxidase (FAD-linked s... 97 2e-21 gi|300313120|ref|YP_003777212.1| glycolate oxidase iron-sulfur s... 76 7e-15 gi|300313341|ref|YP_003777433.1| FAD-dependent D-lactate dehydro... 66 6e-12 >gi|300312605|ref|YP_003776697.1| FAD/FMN-containing dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 238 bits (608), Expect = 6e-64, Method: Compositional matrix adjust. Identities = 165/435 (37%), Positives = 235/435 (54%), Gaps = 13/435 (2%) Query: 34 RYGAGQALCVARPANVAEAAELMRLCAAENIRLIPQGANTGLVGAASPDSSGLQLILSME 93 RY G+AL V RPA+ AE A ++RLCA + L+PQG NTGLV + PD G ++LS+ Sbjct: 41 RY-TGRALAVLRPADSAEVAAVVRLCAQFAVPLVPQGGNTGLVLGSVPDQQGNAVVLSLR 99 Query: 94 RIKGVIDIDPVDGVIQAWAGTRLSDLNQALAPHGLCFPIDLGADPS--VGGMLAANTGGA 151 R+ + +D ++ I +G L L + A G FP+ L A+ S +GG L+ N GG Sbjct: 100 RLNRIRAVDALNNTITVESGCVLQHLQEQAAAAGRLFPLSLAAEGSCTIGGNLSTNAGGT 159 Query: 152 RLIRYGDVRHNTLGLQALLMQPPGELLEMGNRLHKNNTGPDWKQLFIGTAGSYGIVTQAV 211 ++RYG+ R LGL+ + Q GE++ L K+NTG D + LFIG G+ G++T AV Sbjct: 160 GVLRYGNTRELCLGLEVVTAQ--GEIMSSLKGLRKDNTGYDLRDLFIGAEGTLGVITAAV 217 Query: 212 LRAHPLPRQRATALIVPSSLEAGLRLLQDAQAQFADFLTAFEGISRNALASVLRHLPQVN 271 ++ P PR + TAL L+LLQ AQA LT FE +S L V +H P+ Sbjct: 218 MKLFPQPRAQVTALAALPHPAQALQLLQLAQAHCGPALTGFELMSDFCLQLVRKHFPEQR 277 Query: 272 APFD-PLPDYALLVELSSSSAASEHFD--LDQLFMAWLERHF-DDA-IDAVVDKPETLWR 326 PFD P P Y LL ELS S + EH + L LE+ DDA I A + + LWR Sbjct: 278 LPFDRPHPQYVLL-ELSDSE-SEEHARALFEGLIGTALEQGLVDDAVIAASLAQSRALWR 335 Query: 327 LRHAISDSVKQEGRIVAFDIAVSRSRMPAFRAEALALVERDFAGAEVFDFGHWGDGGVHF 386 LR +IS + EG+ + DI+V SR+ F A LV++ G + FGH GDG +H+ Sbjct: 336 LRESISSAQAHEGKNIKHDISVPISRIAEFIAATDVLVQQAAPGCRMVTFGHLGDGNLHY 395 Query: 387 NLAIPPAQQPQFPPERIEALRTALYDLVAKSYQGSFSAEHGIGPFNQHYYQRYTQPEQRA 446 N++ P + R A+ ++D V + + GS SAEHG+G + RY P + Sbjct: 396 NVSPPEGVADRDFIARQAAINRVVHDSVDR-FGGSISAEHGLGALKREEITRYKSPVELQ 454 Query: 447 LASRVQAVFDPHCLL 461 L ++ DP L+ Sbjct: 455 LMRAIKLALDPQQLM 469 >gi|300313123|ref|YP_003777215.1| D-lactate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 471 Score = 124 bits (310), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 114/428 (26%), Positives = 185/428 (43%), Gaps = 46/428 (10%) Query: 51 EAAELMRLCAAENIRLIPQGANTGLVGAASPDSSGLQLILSMERIKGVIDIDPVDGVIQA 110 E A ++LC+ + +IP GA T L G G+ + LS ++ V+ + D Sbjct: 63 EVAAFVKLCSQYDTPIIPYGAGTSLEGHVLALQGGVTVDLS--QMNQVLAVHAEDLTATV 120 Query: 111 WAGTRLSDLNQALAPHGLCFPIDLGADPSVGGMLAANTGGARLIRYGDVRHNTLGLQALL 170 AG LNQ + GL FPID GAD S+GGM + G +RYG ++ NTL L + Sbjct: 121 QAGVTRKQLNQEIKDTGLFFPIDPGADASLGGMASTRASGTNAVRYGTMKENTLTLTVVT 180 Query: 171 MQPPGELLEMGNRLHKNNTGPDWKQLFIGTAGSYGIVTQAVLRAHPLPRQRATALI-VPS 229 Q GE+++ G R K++ G D ++++G+ G+ GI+T+ +R +P P + A+ P+ Sbjct: 181 AQ--GEIIKTGTRAKKSSAGYDLTRVYVGSEGTLGIITEVTVRLYPQPEAISAAICSFPT 238 Query: 230 SLEAGLRLLQDAQAQFADFLTAFEGISRNALASVLRHLPQVNAPFDPLPDYALLVEL-SS 288 +A ++Q Q L E + N + ++ H +++ P +PL LL E S Sbjct: 239 VADAVNTVIQTIQMGVP--LARVELLDENGVRAINAH-DKLSLPVNPL----LLFEFHGS 291 Query: 289 SSAASEHFDLDQ--------LFMAWLERHFDDAIDAVVDKPETLWRLRHAISDSVKQ--- 337 + E +L Q L W R D LW RH ++ Q Sbjct: 292 ENGVKEQAELVQDIAKEFHALGFEWATRPEDRT---------RLWTARHNAYFALLQLRP 342 Query: 338 EGRIVAFDIAVSRSRMPAFRAEALALVERDFA--GAEVFDFGHWGDGGVHFNLAIPPAQQ 395 R ++ D V SR+ AE + + D G GH GDG H + + P Sbjct: 343 GARAISTDCCVPISRL----AECVLATKADCEEQGLTHAIIGHVGDGNFHVQMMVDPND- 397 Query: 396 PQFPPERIEALRTALYDLVAKS--YQGSFSAEHGIGPFNQHYYQRYTQPEQRALASRVQA 453 P I +VA++ G+ + EHG+G + + A+ ++ Sbjct: 398 ----PADIARAEGVNQRMVARAIGMDGTCTGEHGVGLHKMDFLIQEHGEGAIAVMRAIKH 453 Query: 454 VFDPHCLL 461 DP ++ Sbjct: 454 ALDPKNIM 461 >gi|300313121|ref|YP_003777213.1| glycolate oxidase (FAD-linked subunit) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 97.4 bits (241), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 106/427 (24%), Positives = 185/427 (43%), Gaps = 23/427 (5%) Query: 41 LCVARPANVAEAAELMRLCAAENIRLIPQGANTGLVGAASPDSSGLQLILSMERIKGVID 100 + V P N A+ +M+ C A ++++P+GA TGL G A+P + G +++S + ++ Sbjct: 53 MVVVLPENEAQVVAIMKTCHALQVQIVPRGAGTGLSGGATPIADG--VVVSTAKFNQILQ 110 Query: 101 IDPVDGVIQAWAGTRLSDLNQALAPHGLCFPIDLGAD--PSVGGMLAANTGGARLIRYGD 158 +D G R +++A APHGL + D + S+GG +A N+GG ++YG Sbjct: 111 MDKYSRTAVVQPGVRNLAISEAAAPHGLYYAPDPSSQIACSIGGNVAENSGGVHCLKYGL 170 Query: 159 VRHNTLGLQALLMQPPGELLEMGNRLHKNNTGPDWKQLFIGTAGSYGIVTQAVLRAHPLP 218 HN L ++ + ++ GE++E+G+ + G D +FIG+ G G+VT+ ++ P P Sbjct: 171 TVHNVLRVRMVTIE--GEVVELGSG-ALDAPGLDLLAVFIGSEGMLGVVTEVTVKLVPKP 227 Query: 219 RQRATALIVPSSLEAGLRLLQDAQAQFADFLTAFEGISRNALASVLRHLPQVNAPFDPLP 278 + + + G + + A A + A G+ S P V A +D Sbjct: 228 QAARVIMASFDDVVKGGNAVANVIA--AGIIPA--GLEMMDKTSSRMVEPFVKAGYDIDA 283 Query: 279 DYALLVELSSSSAASEHFDLDQLFMAWLERHFDDAIDAVVDKPETLWRLRHAISDSVKQE 338 + LL E S E + L AI + E L R ++ Sbjct: 284 EAILLCE--SDGTVEEVEEEIGRMTDVLNASGATAIACSQSEAERL-RFWSGRKNAFPAA 340 Query: 339 GRI----VAFDIAVSRSRMPAFRAEALALVERDFAGAEVFDFGHWGDGGVHFNLAIPPAQ 394 GRI D + R ++ A +A +E+ + G + H GDG +H + Sbjct: 341 GRISPDYYCMDGTIPRKKL-AQVLLGIAEMEKKY-GLRCANVFHAGDGNLHPLILFDANI 398 Query: 395 QPQFPPERIEALRTALYDLVAKSYQGSFSAEHGIGPFNQHYYQRYTQPEQRALASRVQAV 454 +F R E + +L + G+ + EHG+G + P +R +++ Sbjct: 399 ADEF--HRAELFGAEILELCVE-VGGTITGEHGVGIEKINSMCVQFSPAERETFFKLKRA 455 Query: 455 FDPHCLL 461 FDP LL Sbjct: 456 FDPAFLL 462 >gi|300313120|ref|YP_003777212.1| glycolate oxidase iron-sulfur subunit oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 75.9 bits (185), Expect = 7e-15, Method: Compositional matrix adjust. Identities = 55/159 (34%), Positives = 78/159 (49%), Gaps = 6/159 (3%) Query: 89 ILSMERIKGVIDIDPVDGVIQAWAGTRLSDLNQALAPHGLCF---PIDLGADPSVGGMLA 145 +L G+ID +P + VI A GT LS+++ ALA H P G +VGGM+A Sbjct: 45 LLDTRAYSGIIDYEPTELVITARCGTPLSEIDAALAQHNQMLAWEPPSFGGAATVGGMVA 104 Query: 146 ANTGGARLIRYGDVRHNTLGLQALLMQPPGELLEMGNRLHKNNTGPDWKQLFIGTAGSYG 205 A G G VR LG A+LM GE L G ++ KN G D +L G+ G+ G Sbjct: 105 AGLSGPARASAGAVRDFVLG--AVLMDAQGEPLHFGGQVMKNVAGYDISRLLAGSLGTLG 162 Query: 206 IVTQAVLRAHPLPRQRATALIVPSSLEAGLRLLQDAQAQ 244 ++ + L+ P P +T + + E LRLL + Q Sbjct: 163 LILEVSLKVLPRPIASSTR-VFEINQEGALRLLNQSAGQ 200 >gi|300313341|ref|YP_003777433.1| FAD-dependent D-lactate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 587 Score = 66.2 bits (160), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 43/158 (27%), Positives = 83/158 (52%), Gaps = 11/158 (6%) Query: 6 TLARLQDLLGNAGLITSAPERQPYEHGPRYGAGQALCVARPANVAEAAELMRLCAAENIR 65 +++L+ ++G+ ++T + + + G RYG G+ L V RP + E ++++C A N+ Sbjct: 14 VISKLEAIVGSKHVLTGDAQTRRFRQGLRYGGGEVLAVVRPGRLLEMWRVLQVCHAANMV 73 Query: 66 LIPQGANTGLVGAASPDSSGLQ---LILSMERIKGVIDIDPVDGVIQAWAGTRLSDLNQA 122 +I Q ANTGL G ++P + G ++++ R+ + +D V+ + G L L + Sbjct: 74 IICQAANTGLTGGSTPIAEGYDRPVVLINTMRLTRLELLDQGQQVL-SQPGVTLDQLERR 132 Query: 123 LA-----PHGLCFPIDLGADPSVGGMLAANTGGARLIR 155 L PH + +GA S+ G + N+GG+ + R Sbjct: 133 LKPLGREPHSVIGSSCIGA--SITGGICNNSGGSLIQR 168 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,516,805 Number of extensions: 64626 Number of successful extensions: 233 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 224 Number of HSP's successfully gapped: 5 Length of query: 467 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 378 Effective length of database: 1,198,455 Effective search space: 453015990 Effective search space used: 453015990 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)