BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0100.p (252 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 226 2e-60 gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogena... 214 4e-57 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 115 5e-27 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 113 2e-26 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 108 4e-25 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 226 bits (575), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 126/250 (50%), Positives = 160/250 (64%), Gaps = 7/250 (2%) Query: 1 MSSKFLNHVSVVTGGSTGIGLAIAQGLLAEGARRVYVTGRSRQSLEAAVAVLGEAAVAVV 60 M+ +F + V VVTGGS GIGLA A+ AEGAR VYV+GR LE AV +G AV V Sbjct: 3 MAKRFEDKVVVVTGGSEGIGLATAKLFAAEGAR-VYVSGRRADKLEQAVREIGNGAVGVP 61 Query: 61 SDVSSLADLEYLRAEIEKRGDRLDSIFANAGIC--EKHPFGSTTEADYDRSFDINVKGVF 118 D + L DL+ L A ++ RLD +FANAG E P G E D+D F++NV+G+ Sbjct: 62 GDAAKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRGLL 121 Query: 119 FTVQTLLPLLRDGGAVILTSSICSGNGMAELSVYNASKAAVRSFARTWTNDLRERKIRVN 178 F+VQ LPL+ GGA++L S+ G S+YNASKAAVRSFAR+WT DL+ER IRVN Sbjct: 122 FSVQKALPLMSQGGAIVLNGSVAGVKGFPGQSLYNASKAAVRSFARSWTADLKERGIRVN 181 Query: 179 ALSPGFTRTPLMDNSLKLDHDQIDRLKEHVAQITPLGYMADPAEIASAGLFLASADARYI 238 ++PG T T LM + L+ D LK Q+ PLG + +P EI A LFLASA++ YI Sbjct: 182 VVAPGGTETRLMRDYLEARPGMEDVLK----QVVPLGRLGEPDEIGRAVLFLASAESSYI 237 Query: 239 TGIELTVDGG 248 G+EL VDGG Sbjct: 238 AGVELFVDGG 247 >gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 214 bits (546), Expect = 4e-57, Method: Compositional matrix adjust. Identities = 125/252 (49%), Positives = 170/252 (67%), Gaps = 3/252 (1%) Query: 1 MSSKFLNHVSVVTGGSTGIGLAIAQGLLAEGARRVYVTGRSRQSLEAAVAVLGEAAVAVV 60 M+ + +++VTGG++GIGLA AQ L A+GA+ V++TGR + L+AAVA +G AA + Sbjct: 1 MNKQLNGKIALVTGGTSGIGLATAQELAAQGAQ-VFITGRRQAELDAAVASIGAAATGIR 59 Query: 61 SDVSSLADLEYLRAEIEKRGDRLDSIFANAGICEKHPFGSTTEADYDRSFDINVKGVFFT 120 +D S L++L+ + A+I K RLD +FANAG + P G+ TE +DR F NV+GV FT Sbjct: 60 ADASVLSELDTVYAQIAKSAGRLDILFANAGGGDMMPLGAITEEHFDRIFGTNVRGVLFT 119 Query: 121 VQTLLPLLRDGGAVILTSSICSGNGMAELSVYNASKAAVRSFARTWTNDLRERKIRVNAL 180 VQ LPLL DG +VILTSS S G A SVY+ASKAAVR+FAR+W DL++R IRVN + Sbjct: 120 VQKALPLLVDGASVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLKDRAIRVNVV 179 Query: 181 SPGFTRTPLMDNSLKLDHDQIDRLKEHVAQITPLGYMADPAEIASAGLFLASADARYITG 240 SPG RTP + + D Q L +++A PLG + +P EI A FLAS A ++ G Sbjct: 180 SPGPIRTPGLGELVPDDARQ--GLFDYLASQVPLGRLGEPQEIGKAVAFLASDAASFVNG 237 Query: 241 IELTVDGGLSQI 252 IEL VDGG++Q+ Sbjct: 238 IELFVDGGMAQV 249 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 115 bits (287), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 76/249 (30%), Positives = 131/249 (52%), Gaps = 13/249 (5%) Query: 7 NHVSVVTGGS--TGIGLAIAQGLLAEGARRVYVTGRSRQSLEAAVAVLGEAAVAVVSDVS 64 + V+++TGG+ G+G + A+ + A+GAR + + R A A LG + +V+DV+ Sbjct: 5 DKVAIITGGAGQNGLGFSTARLMAAQGAR-IVILDLERAEPAGAAARLGAQHLGLVADVT 63 Query: 65 SLADLEYLRAEIEKRGDRLDSIFANAGICEKHPFGSTTEADYDRSFDINVKGVFFTVQTL 124 A E A + K+ R+D + NAG+ + T DYDR D++++G + Q + Sbjct: 64 DKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTLYMSQAV 123 Query: 125 LPLL--RDGGAVILTSSICS--GNGMAELSVYNASKAAVRSFARTWTNDLRERKIRVNAL 180 LP + + G+++ SS+ + G G+ Y+A+KA V AR + + IRVN + Sbjct: 124 LPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQGIRVNCI 183 Query: 181 SPGFTRTPLMDNSLKLDHDQIDRLKEHVAQITPLGYMADPAEIASAGLFLASADARYITG 240 +PG T + + L + K +AQ PLG + P ++ +FLAS ++Y TG Sbjct: 184 TPGLISTDINKDILSAEK------KAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTG 237 Query: 241 IELTVDGGL 249 + L V+GG+ Sbjct: 238 VTLDVNGGM 246 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 113 bits (282), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 82/249 (32%), Positives = 131/249 (52%), Gaps = 13/249 (5%) Query: 7 NHVSVVTGGS--TGIGLAIAQGLLAEGARRVYVTGRSRQSLEAAVAVLGEAAVAVVSDVS 64 + V+V+TGG+ G+G A A+ L+AE RV V ++ AA A +G + +V+DV+ Sbjct: 5 DKVAVITGGAGINGLGYATAR-LMAEQGARVVVLDLAQADPAAAAARIGSQHLGLVADVT 63 Query: 65 SLADLEYLRAEIEKRGDRLDSIFANAGICEKHPFGSTTEADYDRSFDINVKGVFFTVQTL 124 A E A I ++ R+D + NAGI + T ADYDR D++++G + Q + Sbjct: 64 DKASCETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRGTLYMSQAV 123 Query: 125 LPLLR--DGGAVILTSSICS--GNGMAELSVYNASKAAVRSFARTWTNDLRERKIRVNAL 180 LP +R G++I SS+ + G G+ Y+A+KA V AR + IRVN++ Sbjct: 124 LPAMRAQQNGSIICISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPEGIRVNSI 183 Query: 181 SPGFTRTPLMDNSLKLDHDQIDRLKEHVAQITPLGYMADPAEIASAGLFLASADARYITG 240 +PG T ++ L + K +A+ PL + +I A +FL S ++Y TG Sbjct: 184 TPGLIGTDIIKGKLT------EEKKAEIAETIPLARLGRADDIGGACVFLGSDLSQYCTG 237 Query: 241 IELTVDGGL 249 I L V+GG+ Sbjct: 238 ITLDVNGGM 246 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 108 bits (271), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 84/248 (33%), Positives = 122/248 (49%), Gaps = 8/248 (3%) Query: 4 KFLNHVSVVTGGSTGIGLAIAQGLLAEGARRVYVTGRSRQSLEAAVAVLG-EAAVAVVSD 62 +F V +VTG ++GIG A A+ EGAR V + R +L L E A +D Sbjct: 11 RFEGKVVIVTGAASGIGEATARRFSDEGAR-VLLADRDAAALGKVFDSLPPERTAARETD 69 Query: 63 VSSLADLEYLRAEIEKRGDRLDSIFANAGICEKHPFGSTTEADYDRSFDINVKGVFFTVQ 122 VS + L +R +LD + ++AG+ + + D+ R NV GVF+ + Sbjct: 70 VSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVNGVFYGAR 129 Query: 123 TLLPLL-RDGGAVILTSSICSGNGMAELSVYNASKAAVRSFARTWTNDLRERKIRVNALS 181 LP L + G ++ +S+ LS YNASK AV + R D + +R+NA+ Sbjct: 130 EALPHLEKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRINAVC 189 Query: 182 PGFTRTPLMDNSLKLDHDQIDRLKEHVAQITPLGYMADPAEIASAGLFLASADARYITGI 241 P T T M + D +D+ E +A LG ADP EIA+ FLAS DA ++ G+ Sbjct: 190 PSLTHT-AMTADMADDPPLLDKFAERIA----LGRGADPLEIAAVITFLASPDASFVNGV 244 Query: 242 ELTVDGGL 249 L VDGGL Sbjct: 245 NLPVDGGL 252 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 649,217 Number of extensions: 23636 Number of successful extensions: 212 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 158 Number of HSP's successfully gapped: 45 Length of query: 252 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 169 Effective length of database: 1,226,865 Effective search space: 207340185 Effective search space used: 207340185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)