BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0099.p (466 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313713|ref|YP_003777805.1| membrane efflux protein [Herbas... 170 2e-43 gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 142 7e-35 gi|300312966|ref|YP_003777058.1| major facilitator superfamily p... 125 8e-30 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 102 7e-23 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 100 2e-22 >gi|300313713|ref|YP_003777805.1| membrane efflux protein [Herbaspirillum seropedicae SmR1] Length = 147 Score = 170 bits (430), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 92/121 (76%), Positives = 104/121 (85%) Query: 296 FIPCGLGFFLGPLMTPAAARRFGRWTPVLGMSLEVIGALLLSAVVAMARDGQAPALAGVI 355 F+PCGLGFF+GPL TPAA RRFG+W VLGM+LE++GAL+LSAVVA GQAPAL V+ Sbjct: 5 FVPCGLGFFVGPLTTPAATRRFGQWVLVLGMALEIVGALVLSAVVATTPAGQAPALFDVV 64 Query: 356 VALGLIGVGQGIAVPTLVRSVIERAPADGSGMVAGIVNSALQISAALGVAVIGSVFFSLA 415 VAL LIG GQGIAVPTLVRS+I+ AP G+GMVAGIVNSALQI+A LGVAV GSVFFSLA Sbjct: 65 VALWLIGFGQGIAVPTLVRSLIDCAPTGGAGMVAGIVNSALQINAVLGVAVTGSVFFSLA 124 Query: 416 G 416 G Sbjct: 125 G 125 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 142 bits (357), Expect = 7e-35, Method: Compositional matrix adjust. Identities = 140/422 (33%), Positives = 207/422 (49%), Gaps = 23/422 (5%) Query: 1 LLVGAFLPPLDFFIVNVALPSIRNGLGADPAEVQLVISGYAAVYAVFLITGGRLGDLLGR 60 L +G + LD +VNV L +I L A + + ++ GY +A L+ GG L D G Sbjct: 35 LAIGFVMAMLDVTVVNVGLSNIAGSLDAPLSTLVWIVDGYTLTFAAMLLVGGALADRFGA 94 Query: 61 KRVFLAGLAGFGLASTLCGVASSPAMLIAGRLLQGLSAAIMAPQGLASIQALFP-ERERA 119 +R++L GL F LAS LCG A + LI RLLQGL AA P L+ + ++ +R RA Sbjct: 95 RRLYLTGLFLFVLASLLCGAAPNGPFLIVARLLQGLGAAFFMPSSLSLLTHVYEDDRVRA 154 Query: 120 RALSMYGATIGLAAIVAQAFGGLLIAANLGDLQWRIIFLINLPIVAIVFLVGIPLLPDTR 179 R L ++ AT+GLAA V GG+LI L WR +FLIN+P+ + ++ L+P Sbjct: 155 RMLGVWSATVGLAAAVGPLVGGVLIHW----LGWRSVFLINVPVGLVGLVMARKLIPQVG 210 Query: 180 SPHAARLDWMGVLLCALTLGLLVVPLVKGPEDGWPWWTCLMLIASPLFA-MAFWRHEQRL 238 HA L+ L + L L L++GP GW L +A L A A RHE Sbjct: 211 G-HARALNLSSHLAGVVMLAGLSFVLIQGPVYGWTSPRILAGLAVALSAGAALIRHE--- 266 Query: 239 TQRGATPLVSPKLARVPGLVVGL-VGVLFFYVVSAFFLTFSIYLQEAIGMSALATGLMFI 297 RG PL+ L P +G L + V +++LQ+ G AL TGL + Sbjct: 267 -LRGIAPLLPKALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGRGADALQTGLQLV 325 Query: 298 PCGLGFFLGPLMTPAAARRFGRWTP-VLGMSLEVIGALLLSAVVAMARDGQAPALAGVIV 356 P F G LM+ + +G P +LG+S+ + A+A G +PA ++ Sbjct: 326 PMMAVIFFGNLMSGRMSAHWGPRVPLLLGLSVGTV-------FTALAM-GFSPATPYWLL 377 Query: 357 ALGLIGVGQGI--AVPTLVRSVIERAPADGSGMVAGIVNSALQISAALGVAVIGSVFFSL 414 AL GI A+P + V++ A + A +N+ QI A +GVA++G++ S+ Sbjct: 378 ALVCASANLGISTAIPAMTTVVMQVAGKAHANSAAAALNANRQIGALVGVALMGAILHSV 437 Query: 415 AG 416 G Sbjct: 438 TG 439 >gi|300312966|ref|YP_003777058.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 544 Score = 125 bits (314), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 126/425 (29%), Positives = 202/425 (47%), Gaps = 27/425 (6%) Query: 1 LLVGAF---LPPLDFFIVNVALPSIRNGLGADPAEVQLVISGYAAVYAVFLITGGRLGDL 57 LL G+F + LD +V + LP++ LGA ++++ V+S Y +A L+ G D Sbjct: 38 LLTGSFGCAMTVLDTNVVGIILPTVARDLGASFSQIEWVVSSYVLCFAALLLPAGATADR 97 Query: 58 LGRKRVFLAGLAGFGLASTLCGVASSPAMLIAGRLLQGLSAAIMAPQGLASI-QALFPER 116 GR+RV L G+A F L S C +A S L R LQG+ AA + LA I A E Sbjct: 98 YGRRRVLLGGIALFALTSLACALAPSAQALYGARALQGVGAAFLLAPALAVIGHAFHDED 157 Query: 117 ERARALSMYGATIGLAAIVAQAFGGLLIAANLGDLQWRIIFLINLPIVAIVFLVGIPLLP 176 +R RA +++G +GL ++A GG++ L WR F +NLP ++ + +P Sbjct: 158 QRERAWALWGGIMGLTMVLAPLIGGVVNTL----LGWRWAFAVNLPACLLLAVAVWRHVP 213 Query: 177 DTRSPHAARLDWMGVLLCALTLGLLVVPLVKGPEDGWPWWTCLMLIASPLFAMAFWRHEQ 236 ++R+P LD +G+L + + LL L+ GPE GW C+ + + Q Sbjct: 214 ESRNPLPRPLDPLGILSSSSAVFLLTWALITGPEHGWTSVACVARGLGGALLLVLFIAWQ 273 Query: 237 RLTQRGATPLVSPKLARVPGLVVGLVGVLFFYVVSAFFLT-FSIYLQEAIGMSALATGLM 295 R R A P++ L R G V + + + + + ++LQ A G+SA TGL Sbjct: 274 R---RAAHPMLELSLFRHAGFVAAVAAMFAYAASAQVMASLLPLFLQNARGLSAWQTGLA 330 Query: 296 FIPCGLGFFLGPLMTPAAARRFGRW--TP---VLGMSLEVIGALLLSAVVAMARDGQAPA 350 +P F L L+ P R RW +P LG+ + +G LL+ + A G A Sbjct: 331 MLP----FALAMLLLPQVGSRLARWLSSPQMLALGLGVTALGNLLM---IQAALSGAA-- 381 Query: 351 LAGVIVALGLIGVGQGIAVPTLVRSVIERAPADGSGMVAGIVNSALQISAALGVAVIGSV 410 A + + ++G G G+ ++++ P + +GM +GI +A G A +G+V Sbjct: 382 -AWTALGMAVLGSGGGLLNGQTQKAIMGNVPRERAGMASGISTTARFTGVLAGFAGLGAV 440 Query: 411 FFSLA 415 A Sbjct: 441 LADTA 445 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 102 bits (254), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 99/340 (29%), Positives = 153/340 (45%), Gaps = 20/340 (5%) Query: 4 GAFLPPLDFFIVNVALPSIRNGLGADPAEVQLVISGYAAVYAVFLITGGRLGDLLGRKRV 63 G F+ LD IVN ALP++ LG + V+ Y A G L D G +R+ Sbjct: 61 GFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFGTRRM 120 Query: 64 FLAGLAGFGLASTLCGVASSPAMLIAGRLLQGLSAAIMAPQGLASIQALFPERERARALS 123 + A + F L S LC +ASS ML+A R++QGL +++ P G ++ P + AL+ Sbjct: 121 YFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIAALA 180 Query: 124 MYGATIGLAAIVAQAFGGLLIAANLGDLQWRIIFLINLPIVAIVFLVGIPLLPDTRSPHA 183 L +V GG L+ + L W IFLINLP+ + + LPD Sbjct: 181 SISIAGQLGPVVGPVLGGWLVES----LSWHWIFLINLPVGVLGMGAVLYFLPDDSLAGT 236 Query: 184 ARLDWMGVLL---CALTLGL-LVVPLVKGPEDGWPWWTCLMLIASPLFAMAFWRHEQRLT 239 R D +G +L C + L L VP + W+ + + + L + +WRH Sbjct: 237 PRFDLLGCVLLSACMVAFSLALDVPF----DSHRTGWSIALFVVAALTVLGYWRHASVHP 292 Query: 240 QRGATPLVSPKLARVPGLVVGLVGVLFFYV-VSAFFLTFSIYLQEAIGMSALATGLMFIP 298 + PL L P +GL G L + SA + LQ +G S L G++ +P Sbjct: 293 R----PLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLP 348 Query: 299 CGL-GFFLGPLMTPAAARRFGRWTPVLGMSLEVIGALLLS 337 + G P + P +RFG + L ++ ++GA ++S Sbjct: 349 AAIAGTIAKPWIAP-LVKRFG-YDRFLLVNTAIVGAAIMS 386 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 100 bits (250), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 84/313 (26%), Positives = 138/313 (44%), Gaps = 14/313 (4%) Query: 10 LDFFIVNVALPSIRNGLGADPAEVQLVISGYAAVYAVFLITGGRLGDLLGRKRVFLAGLA 69 +D +++ +LP++ + DP ++L ++ Y AVF+ G + D G +F + + Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 70 GFGLASTLCGVASSPAMLIAGRLLQGLSAAIMAPQGLASIQALFPERERARALSMYGATI 129 F L S LCGV+S+ +A R LQG+ A+M P G I + +ALS Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 130 GLAAIVAQAFGGLLIAANLGDLQWRIIFLINLPIVAIVFLVGIPLLPDTRSPHAARLDWM 189 ++ GG + WR IF IN+PI + + +PD ++ A LD Sbjct: 121 LFGPVLGPPLGGFITTY----FHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLK 176 Query: 190 GVLLCALTLGLLVVPLVKGPED---GWPWWTCLMLIASPLFAMAFWRHEQRLTQRGATPL 246 G LL AL LL++ L G TC++ A +F A+ H +R + PL Sbjct: 177 GFLLSALGCSLLMMGLATAGRHLVSGEVALTCVL--AGLVFLAAYVWH----ARRASAPL 230 Query: 247 VSPKLARVPGLVVGLVGVLFFYV-VSAFFLTFSIYLQEAIGMSALATGLMFIPCGLGFFL 305 + L ++P L G+ G F+ V + A + LQ G + +G++ G Sbjct: 231 IDLALLKIPTLRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMF 290 Query: 306 GPLMTPAAARRFG 318 + RR+G Sbjct: 291 MKTIAARFLRRYG 303 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.141 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,257,950 Number of extensions: 47399 Number of successful extensions: 447 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 425 Number of HSP's successfully gapped: 16 Length of query: 466 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 377 Effective length of database: 1,198,455 Effective search space: 451817535 Effective search space used: 451817535 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)