BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0075.p (676 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspi... 891 0.0 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 327 2e-90 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 298 1e-81 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 266 4e-72 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 258 1e-69 >gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 706 Score = 891 bits (2303), Expect = 0.0, Method: Compositional matrix adjust. Identities = 479/585 (81%), Positives = 526/585 (89%), Gaps = 1/585 (0%) Query: 1 MSASFHFLLLAFALLVASLSAYAALEVSERIVLQESQHRRLWLAIGAVVFGLGVWGAMLL 60 MS SFHFLLLAFA+LVASLSAYAALEVSER+V+QESQHRRLWL++GAVV GLGVW + L Sbjct: 1 MSGSFHFLLLAFAVLVASLSAYAALEVSERMVMQESQHRRLWLSVGAVVLGLGVWATLFL 60 Query: 61 TVASLDAPMPIAFDARMVVLTLLLCLSSAFYLMHLTGMRRPQPARLVIGGLAIGAAFNAA 120 TVA+L MP+ FD R+ VL LLLCL SAFYLMHL+G+RRP P+RL IGGL+IGAA NAA Sbjct: 61 TVAALGLSMPVGFDIRLTVLALLLCLGSAFYLMHLSGLRRPHPSRLAIGGLSIGAAINAA 120 Query: 121 FHIALKSMTLVPPLVYHTPLLIVALVVSEVVAVFIVTLFSASNGRKPMTGSLNSQQARRV 180 FH+AL +M L+P L+Y LL +AL+++E++ VFIV LFSASNGRKP GSLNSQQ RRV Sbjct: 121 FHMALAAMQLLPALLYQQVLLGLALLLTELIGVFIVALFSASNGRKPKAGSLNSQQTRRV 180 Query: 181 LAALLVGAGLVSSAAAGLRALLIDPQAQSLAGEGITREHLVNGLVFLAFLAMAMALVVSG 240 LAALLVG GLV++A AGLRAL+I P QSLA + REHLVNG+VFLAFLAMA+ALV+SG Sbjct: 181 LAALLVGLGLVAAATAGLRALVIAPHTQSLAVASLLREHLVNGIVFLAFLAMALALVISG 240 Query: 241 VQRNQVLKKIVGRTNDKLLHFATHDVLTGLPNRALLADRIQHAVEVARRNGKPFAVLFMD 300 VQRNQVLK+IVGRTNDKLLHFATHDVLTGLPNRALLADRIQHAVEVARRNGK FAVLFMD Sbjct: 241 VQRNQVLKRIVGRTNDKLLHFATHDVLTGLPNRALLADRIQHAVEVARRNGKTFAVLFMD 300 Query: 301 LDGFKAINDSLGHAVGDGLLVAVAQRIRACIRGEDMVARIGGDEFVVVMSNLSTPEVVEQ 360 LDGFKAINDSLGHAVGDGLLVAVAQRIR C+RGEDMVARIGGDEFVVV+ NLS+P+VVEQ Sbjct: 301 LDGFKAINDSLGHAVGDGLLVAVAQRIRQCLRGEDMVARIGGDEFVVVVGNLSSPDVVEQ 360 Query: 361 LSENILAELRLDFQIEDATLRVTSSIGIAVYPNSGESVDALMKNADAAMYEAKQSGRNTY 420 LSENILA LR DFQI+DATLRVTSSIGIAVYPNSG+SVDALMKNADAAMYEAKQSGRNTY Sbjct: 361 LSENILAALRQDFQIDDATLRVTSSIGIAVYPNSGDSVDALMKNADAAMYEAKQSGRNTY 420 Query: 421 RFFEPAMHASAMRHLQVRQALQQAIDQQQFRLHFQPKYRGAGKELTGLEALLRWHHPELG 480 RFFEPAMHASAMRHLQVRQALQQAID+QQFRLHFQPKYRGA KELTGLEALLRW HP+LG Sbjct: 421 RFFEPAMHASAMRHLQVRQALQQAIDEQQFRLHFQPKYRGASKELTGLEALLRWTHPQLG 480 Query: 481 EMAPSEFIPIAERSGQILCIGDWVLHAVCRQIVCWDAAGVKPVKVALNLSPLQMRSDLVE 540 EMAP+EFIPIAERSGQILCIGDWVL AVC QI WDAAG+KPVKVALNLSPLQMR+DLV Sbjct: 481 EMAPTEFIPIAERSGQILCIGDWVLQAVCEQIARWDAAGMKPVKVALNLSPLQMRTDLVA 540 Query: 541 RIIKVVSASGIAPQRLMFEITETAAMKDVEISXHARGARTAVAGF 585 RI+++V A+GIAPQRLMFEITETAAMKDVE S G A+ GF Sbjct: 541 RIVELVGAAGIAPQRLMFEITETAAMKDVERSQRVIGELQAL-GF 584 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 327 bits (838), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 212/569 (37%), Positives = 322/569 (56%), Gaps = 23/569 (4%) Query: 17 ASLSAYAALEVSERIVLQESQH---RRLWLAIGAVVFGLGVWGAMLLTVASLDAPMPIAF 73 A L++Y AL+++ERI SQH R WLA GAV GLG+W + + + P+ + + Sbjct: 18 AILASYTALDMAERINTTASQHGRAARWWLAGGAVAMGLGIWSMHFIGMLAFRLPIALGY 77 Query: 74 DARMVVLTLLLCLS-SAFYLMHLTGMRRPQ-PARLVIG-GLAIGAAFNAAFHIALKSMTL 130 DA + L+L++ ++ S F L +T RP+ P R ++G L +G + + ++ + Sbjct: 78 DAGITALSLVIAIAVSGFALWIVT---RPELPTRRLLGSALFMGIGIACMHYTGMAALRM 134 Query: 131 VPPLVYHTPLLIVALVVSEVVAVFIVTLFSASNGRKPMTGSLNSQQARRVLAALLVGAGL 190 P + Y L + ++ ++ + + + + P +A R A++++G + Sbjct: 135 QPGIAYDLFLFLASVAIAIAASAAALWIAFSLRRNTPHV------RAARGGASVVMGIAI 188 Query: 191 VSSAAAGLRALLIDPQAQSLAG-EGITREHLVNGLVFLAFLAMAMALVVSGVQ-----RN 244 G+ A + L+ EG+ L ++ + A+ +AL+ S + R Sbjct: 189 FGMHYTGMGAASFSRDSICLSAREGVAPGWLAVLIIVVTLAALTIALLTSVLDARLESRT 248 Query: 245 QVLKKIVGRTNDKLLHFATHDVLTGLPNRALLADRIQHAVEVARRNGKPFAVLFMDLDGF 304 L + + N++L HD LT LPNR LL DR+ A+ A R FA++FMDLDGF Sbjct: 249 AKLARSLAAANEELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFALMFMDLDGF 308 Query: 305 KAINDSLGHAVGDGLLVAVAQRIRACIRGEDMVARIGGDEFVVVMSNLSTPEVVEQLSEN 364 KAINDSLGH VGD LLV VAQR+ + R D VAR+GGDEFV+++ +L+TPE ++E Sbjct: 309 KAINDSLGHHVGDRLLVEVAQRLTSSSRANDTVARLGGDEFVILV-DLNTPEDAMGVAEK 367 Query: 365 ILAELRLDFQIEDATLRVTSSIGIAVYPNSGESVDALMKNADAAMYEAKQSGRNTYRFFE 424 ++ + F+++ LRV++S+GIA+YP G + L+ NADAAMY K+SGRN Y FFE Sbjct: 368 LVEAVNQPFKVDQHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGRNGYHFFE 427 Query: 425 PAMHASAMRHLQVRQALQQAIDQQQFRLHFQPKYRGAGKELTGLEALLRWHHPELGEMAP 484 P+M+A+A + LQ Q L+ A+++ QFRL +QPK+ + G EALLRW HP G +AP Sbjct: 428 PSMNANAQQQLQWLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHPVHGLVAP 487 Query: 485 SEFIPIAERSGQILCIGDWVLHAVCRQIVCWDAAGVKPVKVALNLSPLQMRS-DLVERII 543 +FI +AERSG IL IG WVL CRQ+ W A G +++NLS LQ L+E + Sbjct: 488 DQFIGLAERSGLILPIGAWVLDEACRQMREWFALGYVDWTMSVNLSALQFADPHLLEVVR 547 Query: 544 KVVSASGIAPQRLMFEITETAAMKDVEIS 572 + + P L E+TE+ AM D E S Sbjct: 548 TTLERHQLPPPCLTLEVTESTAMHDTEAS 576 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 298 bits (763), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 151/317 (47%), Positives = 209/317 (65%), Gaps = 1/317 (0%) Query: 257 KLLHFATHDVLTGLPNRALLADRIQHAVEVARRNGKPFAVLFMDLDGFKAINDSLGHAVG 316 ++ H ATHD LTGLPNR +L + +Q A+ VAR+ G AV F+DLD FK INDSLGH VG Sbjct: 261 EMRHQATHDALTGLPNRNMLHEHLQRALRVARQKGALTAVAFVDLDQFKFINDSLGHQVG 320 Query: 317 DGLLVAVAQRIRACIRGEDMVARIGGDEFVVVMSNLSTPEV-VEQLSENILAELRLDFQI 375 D LL VAQR++AC+R DMVAR GGDEFV+V+ N + E + ++ + ILA + +Q Sbjct: 321 DELLKTVAQRLQACLREADMVARQGGDEFVLVLQNQTEGEAGIAEVMQRILAAVARPWQA 380 Query: 376 EDATLRVTSSIGIAVYPNSGESVDALMKNADAAMYEAKQSGRNTYRFFEPAMHASAMRHL 435 D +VT+SIG++ YP G+ V++L+K+AD+AMY AK+ GRN ++FF P M + L Sbjct: 381 GDREFQVTASIGVSRYPADGKDVESLLKHADSAMYRAKEQGRNNFQFFAPWMDSQVSNRL 440 Query: 436 QVRQALQQAIDQQQFRLHFQPKYRGAGKELTGLEALLRWHHPELGEMAPSEFIPIAERSG 495 ++ L++A+DQ +F+L++QPK L G EAL+RW P+ G + P FIP AE +G Sbjct: 441 EMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWESPQQGMVPPDRFIPFAEEAG 500 Query: 496 QILCIGDWVLHAVCRQIVCWDAAGVKPVKVALNLSPLQMRSDLVERIIKVVSASGIAPQR 555 I+ IG+WVL CRQ W AG+ P+ VA+NLSP Q+ L + + V++ SG+A Sbjct: 501 LIVPIGEWVLRTACRQNQAWQQAGLPPIPVAVNLSPRQLNQSLPDFVAGVLAQSGLAASW 560 Query: 556 LMFEITETAAMKDVEIS 572 L EITE MKD E S Sbjct: 561 LELEITENVVMKDAEKS 577 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 266 bits (680), Expect = 4e-72, Method: Compositional matrix adjust. Identities = 138/323 (42%), Positives = 200/323 (61%), Gaps = 8/323 (2%) Query: 255 NDKLLHFATHDVLTGLPNRALLADRIQHAVEVARRNGKPFAVLFMDLDGFKAINDSLGHA 314 +++ A D LT LPNR LL DR++ A+ ++ R A+LF+DLD FK +ND++GH Sbjct: 592 EERIRELAFFDALTRLPNRTLLLDRLKQAIALSLRTKTCAALLFIDLDHFKTLNDTIGHE 651 Query: 315 VGDGLLVAVAQRIRACIRGEDMVARIGGDEFVVVMSNLST--PEVVEQ---LSENILAEL 369 GD LL VAQR+ A +R D VAR+GGDEFVVV+ L+ E Q + E IL L Sbjct: 652 KGDTLLKQVAQRLLANVRENDTVARVGGDEFVVVLEGLNELQQEAATQTRAIGEKILTAL 711 Query: 370 RLDFQIEDATLRVTSSIGIAVYPNSGESVDALMKNADAAMYEAKQSGRNTYRFFEPAMHA 429 +Q++D R T+SIG V+ SVD LMK AD AMY+AK++GRN RFF+PAM Sbjct: 712 GSTYQLDDVEYRTTASIGATVFLGRAASVDELMKQADLAMYKAKETGRNALRFFDPAMQT 771 Query: 430 SAMRHLQVRQALQQAIDQQQFRLHFQPKYRGAGKELTGLEALLRWHHPELGEMAPSEFIP 489 + + + L++AID Q LH+QP+ G+ +TG E L+RW HP+ G + P +FIP Sbjct: 772 AVLERATLEAGLRKAIDGNQLLLHYQPQVVEGGR-VTGAEVLVRWQHPQRGMVPPGDFIP 830 Query: 490 IAERSGQILCIGDWVLHAVCRQIVCWDAA-GVKPVKVALNLSPLQMRS-DLVERIIKVVS 547 +AE +G IL +G+WVL CRQ+ W A + + +A+N+S Q+R D V+ ++ + Sbjct: 831 LAEETGLILSLGNWVLETACRQLATWAAQPQMADLSIAVNVSAPQLREPDFVQTVLDTIE 890 Query: 548 ASGIAPQRLMFEITETAAMKDVE 570 +G P+RL E+TE+ + +VE Sbjct: 891 RTGANPRRLKLELTESLLVDNVE 913 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 258 bits (658), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 134/317 (42%), Positives = 194/317 (61%), Gaps = 4/317 (1%) Query: 262 ATHDVLTGLPNRALLADRIQHAVEVARRNGKPFAVLFMDLDGFKAINDSLGHAVGDGLLV 321 A+ D LTGL NR +L ++ A+ VA R + V+F+DLD FK +ND+LGH GD LL Sbjct: 519 ASRDTLTGLINRHMLRHHLEQAIGVAERQKQALWVVFVDLDRFKFVNDTLGHKAGDTLLR 578 Query: 322 AVAQRIRACIRGEDMVARIGGDEFVVVMSNLSTPEVVEQLSENILAELRLDFQIEDATLR 381 VA R+RA +R D VAR+GGDEF++++ + + + I+ + + TL Sbjct: 579 TVAGRLRAAVREADTVARLGGDEFIMILPEQDELTLDTRSLQRIMDAVAAPVTLGGHTLF 638 Query: 382 VTSSIGIAVYPNSGESVDALMKNADAAMYEAKQSGRNTYRFFEPAMHASAMRHLQVRQAL 441 +T S+G+A YPN G + L+K+AD AMY KQ+GRN ++F+ PAM+ A+ L++ L Sbjct: 639 MTCSVGVACYPNDGTDGETLIKHADIAMYRGKQTGRNNFQFYTPAMNEQALERLRIEGDL 698 Query: 442 QQAIDQQQFRLHFQPKYRGAGKELTGLEALLRWHHPELGEMAPSEFIPIAERSGQILCIG 501 + A+D +F LH+QPK A + G EAL+RW HP+LG ++P+ FI +AE +G IL IG Sbjct: 699 RNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQLGMVSPARFIGLAEETGLILPIG 758 Query: 502 DWVLHAVCRQIVCWDAAGVKPVKVALNLSPLQ-MRSDLVERIIKVVSASGIAPQRLMFEI 560 WVL A C Q W G + V++NLS Q ++ DLV I V+ +G+ P+ L E+ Sbjct: 759 AWVLRAACTQCRQWQLGGYADISVSVNLSVGQFLQKDLVSSIADVLEDTGLPPESLELEL 818 Query: 561 TETAAMKDVEISXHARG 577 TE+ M DVE HA G Sbjct: 819 TESLMMTDVE---HAIG 832 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.137 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,565,105 Number of extensions: 58332 Number of successful extensions: 415 Number of sequences better than 1.0e-08: 44 Number of HSP's gapped: 346 Number of HSP's successfully gapped: 44 Length of query: 676 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 584 Effective length of database: 1,184,250 Effective search space: 691602000 Effective search space used: 691602000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 134 (56.2 bits)