BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0060.p (704 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310177|ref|YP_003774269.1| C4-dicarboxylate transport sens... 1274 0.0 gi|300313806|ref|YP_003777898.1| PAS/PAC HisKA ATPase_c domains ... 154 3e-38 gi|300310677|ref|YP_003774769.1| two component transmembrane sen... 139 9e-34 gi|300312655|ref|YP_003776747.1| C4-dicarboxylate transport sens... 114 3e-26 gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine... 106 6e-24 >gi|300310177|ref|YP_003774269.1| C4-dicarboxylate transport sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 674 Score = 1275 bits (3298), Expect = 0.0, Method: Compositional matrix adjust. Identities = 627/656 (95%), Positives = 646/656 (98%) Query: 46 SQLQASGRKTTMRWLIPFLLVCLFLATLIWLPRQAQQMEASERLEQLIADSLWVEQAIRF 105 SQL+ASGRKTTMRWLIPFLLVCLFLATLIWLPRQAQQMEASERLEQLIADSLWVEQAIRF Sbjct: 17 SQLRASGRKTTMRWLIPFLLVCLFLATLIWLPRQAQQMEASERLEQLIADSLWVEQAIRF 76 Query: 106 QLSRDDESMRIIGREISNGRLHQDHFRSRLNALLRNNREFYRVTWFNAEGHPVASTDDLP 165 QLSRDDESMRIIGREI+NGRLHQ+HFRSRLNALLRNNREFYRVTWFNAEG PVASTDD P Sbjct: 77 QLSRDDESMRIIGREITNGRLHQEHFRSRLNALLRNNREFYRVTWFNAEGRPVASTDDFP 136 Query: 166 ISRKDLSQPSLMTADRARQMRRPLYSPPAIPPGIAEPMLMDYHVPLFHDNHYIGSLVASY 225 I+R DLS+ SLMT +RARQMRRPLYSPPA+PPGIAEPMLMDYHVPLFHDNHY+GSLVASY Sbjct: 137 ITRADLSKSSLMTGERARQMRRPLYSPPAVPPGIAEPMLMDYHVPLFHDNHYVGSLVASY 196 Query: 226 AVARILNEMVPWWFAQDNEISLTDTDDNVVARRTSGGPGLNVYTHRRELELPGLTLVLHT 285 AVARILNEMVPWWFAQDNEISLTDTDDNVVARRTSGGPGLNVYTHRRELELPGLTLVLHT Sbjct: 197 AVARILNEMVPWWFAQDNEISLTDTDDNVVARRTSGGPGLNVYTHRRELELPGLTLVLHT 256 Query: 286 NSTKSAPKLLPNLLVGSVIVLSLGLLWSLWALWRDINRRTKAEKALLNEAVFRAAMENSL 345 NSTKSAPKLLPNLLVGSVIVLSLGLLWSLWALWRDINRRTKAEKALLNEAVFRAAMENSL Sbjct: 257 NSTKSAPKLLPNLLVGSVIVLSLGLLWSLWALWRDINRRTKAEKALLNEAVFRAAMENSL 316 Query: 346 VTGMRARDLQGRITYVNPAFCKMVGFAAEELVGKIPPMPYWAPEAMDEYQERFSNVLAGR 405 VTGMRARDL+GRITYVNPAFCKMVGFAAEELVGKIPPMPYWAPEAMDEYQERFSNVLAGR Sbjct: 317 VTGMRARDLEGRITYVNPAFCKMVGFAAEELVGKIPPMPYWAPEAMDEYQERFSNVLAGR 376 Query: 406 ATPQFETIFQRRDGERFPVLIFESPLVDQDGKQTGWMGSILDISERKRAEDLNRTQEEKL 465 ATPQFETIFQRRDGERFPVLIFESPLVDQDGKQTGWMGSILDISERKRAEDLNRTQEEKL Sbjct: 377 ATPQFETIFQRRDGERFPVLIFESPLVDQDGKQTGWMGSILDISERKRAEDLNRTQEEKL 436 Query: 466 QASARLASMGEIASTLAHELNQPLAAISSYTTGALNMMQQGQADPKALQPALEKIHTQAQ 525 QASARLASMGEIASTLAHELNQPLAAISSYTTGALN+MQQGQADPKALQPALEKIH QAQ Sbjct: 437 QASARLASMGEIASTLAHELNQPLAAISSYTTGALNLMQQGQADPKALQPALEKIHAQAQ 496 Query: 526 RAGHVIRSVHQFVKKREPARQRLQIRQLVDNVAPLIELQAQQFFVVLQWQVPKSLPPVLG 585 RAGHVIRSVHQFVKKREPARQR+Q+RQL+DNVAPLIELQAQQFFVVLQ QVP+SLPPVLG Sbjct: 497 RAGHVIRSVHQFVKKREPARQRVQMRQLIDNVAPLIELQAQQFFVVLQLQVPRSLPPVLG 556 Query: 586 DQVLLEQVILNLTRNGIQAMAAVPSARRILRVTAELTRNDDGAESVTVSVIDQGHGIAEE 645 DQ+LLEQVILNLTRNGIQAMAAVPSARRILRV AE+TRN+DGAESVTVSV DQGHGI ++ Sbjct: 557 DQILLEQVILNLTRNGIQAMAAVPSARRILRVAAEVTRNEDGAESVTVSVTDQGHGIPDD 616 Query: 646 VAARLFSPFFSTKAEGMGMGLNICRTTIEFHGGTLTYTDNPAGGTIFRFSLPAQSG 701 VAARLFSPFFSTKAEGMGMGLNICRTTIEFHGGTLTYTDNPAGGTIFRFSLPAQSG Sbjct: 617 VAARLFSPFFSTKAEGMGMGLNICRTTIEFHGGTLTYTDNPAGGTIFRFSLPAQSG 672 >gi|300313806|ref|YP_003777898.1| PAS/PAC HisKA ATPase_c domains containing sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 524 Score = 154 bits (388), Expect = 3e-38, Method: Compositional matrix adjust. Identities = 100/341 (29%), Positives = 170/341 (49%), Gaps = 5/341 (1%) Query: 358 ITYVNPAFCKMVGFA-AEELVGKIPPMPYWAPEAMDEYQERFSNVLAGRATPQFETIFQR 416 + Y + C++ G A + P+ E E + +A T + E R Sbjct: 185 LMYCSDELCRIYGLAPGRNCITYEEFAAIMHPDDRQEVVETVNAAVAMGGTIRVEHRICR 244 Query: 417 RDGERFPVLIFESPLVDQDGKQTGWMGSILDISERKRAEDLNRTQEEKLQASARLASMGE 476 +D L + +DG T ++G+ DI+ R++ E R + ++ +R ++G+ Sbjct: 245 QDTGEVRYLSGIGKPIWRDGTFTEYVGTATDITVRRQNEYAIRAAQIDMERVSRANTVGQ 304 Query: 477 IASTLAHELNQPLAAISSYTTGALNMMQQGQADPKALQPALEKIHTQAQRAGHVIRSVHQ 536 + +++AHE+NQPL +I + +L + + D + + L I + QRAG +I + Sbjct: 305 LTASIAHEINQPLMSIVANAGASLRWLDRPVPDLEHARTGLRDIIAEGQRAGGIISGLRD 364 Query: 537 FVKKREPARQRLQIRQLVDNVAPLIELQAQQFFVVLQWQVPKSLPPVLGDQVLLEQVILN 596 + P +R+ + ++ ++ L + ++ V L + + VLGDQV L+QV+LN Sbjct: 365 LTRNAAPVCERVNLHAVIRHILTLSRSELERREVSLLLSLTPADAFVLGDQVQLQQVMLN 424 Query: 597 LTRNGIQAMAAVPSARRILRVTAELTRNDDGAESVTVSVIDQGHGIAEEVAARLFSPFFS 656 L N I+AM+++P R+L +TA + D+ + + V V D G GIA E R+F F++ Sbjct: 425 LVVNAIEAMSSMPEHARVLSITA--SSCDE--QRIEVKVQDSGEGIAPEAMRRVFDAFYT 480 Query: 657 TKAEGMGMGLNICRTTIEFHGGTLTYTDNPAGGTIFRFSLP 697 TK GMGMGL ICR+ IE H G L G +F F LP Sbjct: 481 TKDSGMGMGLAICRSIIENHRGRLRVAAAQPHGALFSFDLP 521 >gi|300310677|ref|YP_003774769.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 508 Score = 139 bits (349), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 109/349 (31%), Positives = 173/349 (49%), Gaps = 18/349 (5%) Query: 356 GRITYVNPAFCKMVGFAAEELVGKIPPMPY---WAPEAMDEYQERFSNVLAGRATPQFET 412 G I Y++ A K++G G+ PP + P++ +E QE + L + Sbjct: 165 GGIEYLSDAGRKLLGVRP----GQNPPKNFVEQCLPDSQEELQELLTLALPDAISFPLRY 220 Query: 413 IFQRRDG-ERFPVLIFESPLVDQDGKQTGWMGSILDISERKRAEDLNRTQEEKLQASARL 471 R+DG R+ + ++ D K G + DI+++ AE+ R + L+ AR Sbjct: 221 RIARKDGLVRWHHAVCRYIEIENDLK--GHLLHFHDITDQVEAEEARRRDQAALEYLARH 278 Query: 472 ASMGEIASTLAHELNQPLAAISSYTTGA---LNMMQQGQADPKALQPALEKIHTQAQRAG 528 +MGE+A+ +AHEL+QPLAAI ++ G+ LN + + + LE Q A Sbjct: 279 NAMGEMATAIAHELSQPLAAIRNFLEGSVLRLNHSEALREQAGQIIWGLESAGRQVDHAA 338 Query: 529 HVIRSVHQFVKKREPARQRLQIRQLVDNVAPLIELQAQQFFVVLQWQVPKSLPPVLGDQV 588 +I+SV ++V K E + + + +++ V IE++A + V L+ Q + V ++V Sbjct: 339 AIIKSVREYVVKLEQSEGLIDLNEVLAEVRWFIEMRAAEGRVQLEVQASEEALVVSCEKV 398 Query: 589 LLEQVILNLTRNGIQAMAAVPSARRILRVTAELTRNDDGAESVTVSVIDQGHGIAEEVAA 648 L+ QVILNL N I+ M+ P +R RV L R D A ++V D+G GI Sbjct: 399 LIGQVILNLAFNAIEEMSHFPENQR--RVCISLARKDQQA---LLTVADEGRGIDAGHRE 453 Query: 649 RLFSPFFSTKAEGMGMGLNICRTTIEFHGGTLTYTDNPAGGTIFRFSLP 697 RLF FFS+K G G+GL +C+ I H G + GT F F+LP Sbjct: 454 RLFDGFFSSKVSGNGIGLALCKNIIARHRGDIWAQPAQVRGTRFCFTLP 502 >gi|300312655|ref|YP_003776747.1| C4-dicarboxylate transport sensor kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 600 Score = 114 bits (284), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 84/284 (29%), Positives = 149/284 (52%), Gaps = 13/284 (4%) Query: 417 RDGERFPVLIFESPLVDQDGKQTGWMGSILDISER-KRAEDLNRTQEEKLQASARLASMG 475 RD R E + ++ G+ T ++ E+ ++ E L RT + +L + +LA +G Sbjct: 321 RDALRRAAAELEQRIAERTGQLTAANQALEARYEKLQQTESLLRTTQNELVQAGKLAMLG 380 Query: 476 EIASTLAHELNQPLAAISSYTTGALNMMQQGQADPKALQPALEKIHTQAQRAGHVIRSVH 535 ++A+ + HELNQPL AI ++ A+ + +GQ+ +A++ LE I + + G +I + Sbjct: 381 QMAAGVTHELNQPLTAIRAFADNAVKFLARGQS-AQAVEN-LEYISSASATMGKIIAQLK 438 Query: 536 QFVKKREPARQRLQIRQLVDNVAPLIELQAQQFFVVLQWQVPKSLPPVLGDQVLLEQVIL 595 F +K A + + Q ++ + L+ + Q+ + + ++ V GD V EQV++ Sbjct: 439 GFARKSGEAVACVDLSQAIEASSLLLASECQKLGATISIDI-RAAACVTGDVVRTEQVLV 497 Query: 596 NLTRNGIQAMAAVPSARRILRVTAELTRNDDGAESVTVSVIDQGHGIAEEVAARLFSPFF 655 NL RN + A+ R + V E+ G ++ V + D G GIA+EVA LF PFF Sbjct: 498 NLLRNALDAVEEAEHKR--IAVVLEVA----GGYAL-VRIRDSGGGIADEVAPHLFEPFF 550 Query: 656 STK--AEGMGMGLNICRTTIEFHGGTLTYTDNPAGGTIFRFSLP 697 +TK ++G+G+GL I + ++ G L+ ++ GG F LP Sbjct: 551 TTKSSSKGLGLGLAISSSIVQAMNGQLSAHNHEEGGAEFVVRLP 594 >gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 552 Score = 106 bits (265), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 90/360 (25%), Positives = 160/360 (44%), Gaps = 31/360 (8%) Query: 358 ITYVNPAFCKMVGFAAEELVGKIPPMPYWAPEAMDEYQERFSNVLAGRATPQFETIFQRR 417 I +VN AF M G+++ E+VGK PE +A R E + R+ Sbjct: 68 IVFVNNAFINMTGYSSAEVVGKNCRF-LQGPETDRSVVAEVRRAVAERREIATELLNYRK 126 Query: 418 DGERFPVLIFESPLVDQDGKQTGWMGSILDISERKRAEDLNRTQEEKLQASARLASMGEI 477 +G F +F SP+ DQ G + S LDIS R+ AE + L + ++ ++G++ Sbjct: 127 NGSTFWNALFISPVYDQQGNLKYFFSSQLDISRRRDAE-------QALGQARKMEALGQL 179 Query: 478 ASTLAHELNQPLAAISSYTTGALNMMQQGQADPKALQPALEKIHTQAQRAGHVIRSVHQF 537 +AH+ N L I+ Y ++ + D + + + I + +A + + + F Sbjct: 180 TGGIAHDFNNLLQVITGYLDMINLALRSDKPDLARVARSADSIRKASGKAAMLTQQLLAF 239 Query: 538 VKKREPARQRLQIRQLVDNVAPLIELQAQQFFVVLQWQVPKSLPPVLGDQVLLEQVILNL 597 +K+ + + + L + L+ + + +Q ++ + L D +E +LN+ Sbjct: 240 ARKQRLEGRTINLNSLTEGFTDLVS-RTLGGDITVQTELARGLWNCRLDPTQMEVALLNV 298 Query: 598 TRNGIQAMAAVPSARRILRVTAELTRNDDGAE-------------SVTVSVIDQGHGIAE 644 N AM + R +R+ T N+D A+ V+++V D G GIA Sbjct: 299 LINARDAM----NGRGRVRIR---TANEDLADPDRAVQLGLKEGLYVSIAVTDDGPGIAP 351 Query: 645 EVAARLFSPFFSTKAE--GMGMGLNICRTTIEFHGGTLTYTDNPAGGTIFRFSLPAQSGQ 702 ++ R+ PFF+TK E G G+GL++ ++ GG++ T GGT PA Q Sbjct: 352 DILPRVMDPFFTTKDEGKGTGLGLSMVYGFVKQSGGSVRITSGAEGGTTVTIYFPATQDQ 411 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,106,621 Number of extensions: 85086 Number of successful extensions: 418 Number of sequences better than 1.0e-08: 24 Number of HSP's gapped: 398 Number of HSP's successfully gapped: 24 Length of query: 704 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 612 Effective length of database: 1,184,250 Effective search space: 724761000 Effective search space used: 724761000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)