BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0053.p (220 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310171|ref|YP_003774263.1| DedA DSG-1 transmembrane protei... 410 e-116 gi|300312735|ref|YP_003776827.1| DedA transmembrane protein [Her... 197 6e-52 gi|300310491|ref|YP_003774583.1| transmembrane protein [Herbaspi... 58 7e-10 >gi|300310171|ref|YP_003774263.1| DedA DSG-1 transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 220 Score = 410 bits (1055), Expect = e-116, Method: Compositional matrix adjust. Identities = 204/220 (92%), Positives = 210/220 (95%) Query: 1 MDYLQWLDVLVHVDKYLGVLIEQYGPLIYAVLFLIVFCETGLVVLPFLPGDSLLFIAGTF 60 MDYLQ LDVLVHVDKYLGVLIEQYG LIYAVLFLIVFCETGLVVLPFLPGDSLLFIAGTF Sbjct: 1 MDYLQLLDVLVHVDKYLGVLIEQYGALIYAVLFLIVFCETGLVVLPFLPGDSLLFIAGTF 60 Query: 61 CALGAMDIWLLMGLLIAAAVAGNTLNYWIGGWVGQRVYTHNYRWLNPDALRKTHTFYEKH 120 CA GAMDIWLLM LL+ AAVAGNTLNYWIGGWVGQRVYTHNYRWLNP+ALRKTH FYEKH Sbjct: 61 CATGAMDIWLLMTLLVVAAVAGNTLNYWIGGWVGQRVYTHNYRWLNPEALRKTHAFYEKH 120 Query: 121 GGKTIVIARFTPIVRTFAPFIAGVSAMSFARFQLFNVLGAFLWVVSLVVLGYLFGNIPVV 180 GGKTIVIARFTPIVRTFAPFIAGVSAMSFARFQLFNVLGAFLWVV LVVLGYLFGNIPVV Sbjct: 121 GGKTIVIARFTPIVRTFAPFIAGVSAMSFARFQLFNVLGAFLWVVGLVVLGYLFGNIPVV 180 Query: 181 RENLNTLVLIGIVGAIVPIVLGALWQLWRKLRGKRKVSNA 220 ++NLNTLVLIGIV A+ PIVLG LWQ+WRKLRGKRKVSNA Sbjct: 181 QQNLNTLVLIGIVAAVFPIVLGGLWQIWRKLRGKRKVSNA 220 >gi|300312735|ref|YP_003776827.1| DedA transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 213 Score = 197 bits (501), Expect = 6e-52, Method: Compositional matrix adjust. Identities = 108/218 (49%), Positives = 149/218 (68%), Gaps = 9/218 (4%) Query: 1 MDYLQWL-DVLVHVDKYLGVLIEQYGPLIYAVLFLIVFCETGLVVLPFLPGDSLLFIAGT 59 MD L +L D +VH+D++L L YGP ++ +LFLI+FCETGLVV P LPGDSLLF+ G Sbjct: 1 MDILLFLVDFIVHIDRHLAELAASYGPWLFLILFLIIFCETGLVVTPILPGDSLLFVTGA 60 Query: 60 FCALGAMDIWLLMGLLIAAAVAGNTLNYWIGGWVGQRVYTH-NYRWLNPDALRKTHTFYE 118 A GA DI L++ LI AA+ G++ NY IG +G +V+ N R + L KTH F+E Sbjct: 61 LAATGAYDINLMVLTLIVAAILGDSTNYQIGKMLGIKVFDKPNSRIFKKEYLDKTHAFFE 120 Query: 119 KHGGKTIVIARFTPIVRTFAPFIAGVSAMSFARFQLFNVLGAFLWVVSLVVLGYLFGNIP 178 KHGGK I+IARF PIVRTFAPF+AGV AM++ RF L+NV+G WV S GY FGN+P Sbjct: 121 KHGGKAIIIARFAPIVRTFAPFVAGVGAMTYTRFFLYNVVGGIAWVASFAYAGYFFGNMP 180 Query: 179 VVRENLNTLVLIGIVGAIVPIVLGALWQLWRKLRGKRK 216 +V++NL+ L++ ++ +I+P ++ +R KR+ Sbjct: 181 IVKKNLSLLIVAIVILSILPGII-------EYIRHKRR 211 >gi|300310491|ref|YP_003774583.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 307 Score = 57.8 bits (138), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 56/206 (27%), Positives = 102/206 (49%), Gaps = 13/206 (6%) Query: 20 LIEQYGPLIYAVLFLIVFCETGLVVLPFLPGDSLLFIAGTFCALGAMDIWLLMGLLIAAA 79 LIEQYG LI +F+ V E +P P L + G A G +L+ + + AA Sbjct: 7 LIEQYGLLI---VFVNVLVEQLGAPVPAYP---TLILTGALSAAGRYSPAVLLLVAVCAA 60 Query: 80 VAGNTLNYWIGGWVGQRVYTHNYR-WLNPDA-LRKTHTFYEKHGGKTIVIARFTPIVRTF 137 + + + Y G G+++ + R L+PD+ +R+T + Y + G +++++A+F P + Sbjct: 61 LLADYVWYLAGRRYGRQIMSRLCRISLSPDSCVRQTESIYLRWGARSLLLAKFIPGFASI 120 Query: 138 APFIAGVSAMSFARFQLFNVLGAFLWVVSLVVLGYLFGNIPVVRENLNTLVLIGIVGAI- 196 A ++G F L++ G+ LW + LG LF + V + LN L +G G + Sbjct: 121 ASALSGTMGTRRRSFLLWDAAGSALWAGLGIFLGSLFSS--AVDDLLNVLAELGHYGMLM 178 Query: 197 VPIVLGALW--QLWRKLRGKRKVSNA 220 + + L A + W++ R ++ + A Sbjct: 179 IAVALAAFVANKWWQRRRFRKSLEMA 204 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.331 0.147 0.479 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 788,938 Number of extensions: 34739 Number of successful extensions: 173 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 171 Number of HSP's successfully gapped: 3 Length of query: 220 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 138 Effective length of database: 1,231,600 Effective search space: 169960800 Effective search space used: 169960800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 129 (54.3 bits)