BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0052.p (998 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310170|ref|YP_003774262.1| diguanylate cyclase/phosphodies... 1755 0.0 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 372 e-104 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 371 e-103 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 367 e-102 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 343 3e-95 >gi|300310170|ref|YP_003774262.1| diguanylate cyclase/phosphodiesterase A protein [Herbaspirillum seropedicae SmR1] Length = 1002 Score = 1755 bits (4546), Expect = 0.0, Method: Compositional matrix adjust. Identities = 848/984 (86%), Positives = 908/984 (92%), Gaps = 1/984 (0%) Query: 13 DHQRLREEEREALLGALWRSMAILEFAADGSILDANDKFLGMLGYQRHEVVGQHHRMLCD 72 DHQRL ER+ALL ALWRSMAILEF+ADG++L+AN+ FL MLGY+R +VVGQHHRMLCD Sbjct: 12 DHQRLSGSERDALLAALWRSMAILEFSADGTVLEANENFLAMLGYRRADVVGQHHRMLCD 71 Query: 73 PLSVETEDYQRFWQHLRQGEVIAGTLQRRRIDGTIVWLEALCSPILDGSGQLRKVISVAA 132 P V+T+ YQ+FW+HLR+GE+IAGTLQRR + G VWLEALCSPILD GQL KVISVAA Sbjct: 72 PAYVQTDAYQQFWEHLRKGELIAGTLQRRHVGGATVWLEALCSPILDAQGQLLKVISVAA 131 Query: 133 DISSHIDDSHHSNNVLSALNRSLGVVELSPQGMILAANRNYLKTMEYLADDLIGQPHSVL 192 DIS+HIDDS HSN++LSALNRSLGVVELSP+G+ILAAN NYLKTMEYLA+DL+GQPHS+L Sbjct: 132 DISAHIDDSRHSNSILSALNRSLGVVELSPEGVILAANGNYLKTMEYLAEDLLGQPHSIL 191 Query: 193 CPPGYADSEEYAIFWRDVCSGRFFSGRFPRRTRSGRTIWWEATYSPLLDPQGKAERVICI 252 C YA SEEY FWRDV GRFFSGRFPRRTRSGRTIWWEATYSPLLD GKAERVICI Sbjct: 192 CEADYAASEEYTCFWRDVSLGRFFSGRFPRRTRSGRTIWWEATYSPLLDAHGKAERVICI 251 Query: 253 GNDVTYRVTREKSDREHLHLLSLGINETDNAVLITDEQNRIVFHNTGFTRMLGFQSQQTI 312 G DVTYRVTREKSDREHLHLLSLGINETDNAVLITD+QNRIVFHNTGFTRMLGF+SQQ I Sbjct: 252 GTDVTYRVTREKSDREHLHLLSLGINETDNAVLITDDQNRIVFHNTGFTRMLGFESQQVI 311 Query: 313 GKLPRELFASHPTFVKSIERCHGELLQGHSFHTEELIHDLHEQPVWISAVVNPILDVQGK 372 G+LP ELFAS PTFVKS+ERCHGEL+QG S+HTEELIHD+ EQPVWISAVVNPILD GK Sbjct: 312 GRLPGELFASRPTFVKSLERCHGELVQGRSYHTEELIHDMQEQPVWISAVVNPILDGAGK 371 Query: 373 LINAVCVMTDITNTKVHEVLQYRVLNAMARDSSLAEQMNLLCEEVERVAPEVIASVLRVD 432 LI+AVCVMTDITNTKVHEVLQYRVLNAMARDSSL+EQMNLLCEEVERVAPEVIASVLRVD Sbjct: 372 LIHAVCVMTDITNTKVHEVLQYRVLNAMARDSSLSEQMNLLCEEVERVAPEVIASVLRVD 431 Query: 433 EQGRLHTLAAPSLPEAYTRAIEGIQIGPLVGSCGSAAYTGESVVSEEIATDPHWADYKEI 492 E G L TLAAPSLPEAY++AI+G+ +GP+VG CGSAAYTG++V+SE+I TDPHW DYK + Sbjct: 432 EHGCLRTLAAPSLPEAYSKAIDGLHVGPMVGCCGSAAYTGQTVISEDIQTDPHWNDYKHL 491 Query: 493 ALSHGLQACWSTPIKSADGKVIGTFGFYYRQPRAPSAFHHLLVDAGLHLCALAIEREASR 552 AL HGL+ACWSTPIKSA G+VIGTFGFYYR P PSAFHHLLV+AGLHLCALAIERE +R Sbjct: 492 ALPHGLRACWSTPIKSASGQVIGTFGFYYRHPGQPSAFHHLLVEAGLHLCALAIEREDAR 551 Query: 553 ARIHQLAFYDSLTGLPNRSLLLVQAGQAIAAAERNRSMVAVMFIDLDRFKQVNDSLGHQA 612 ARIHQLAFYDSLTGLPNRSLLLVQAGQAIAAAERN S VAVMFIDLDRFKQVNDSLGHQA Sbjct: 552 ARIHQLAFYDSLTGLPNRSLLLVQAGQAIAAAERNCSTVAVMFIDLDRFKQVNDSLGHQA 611 Query: 613 GDELLRTVATRLQSQARRSDIVGRLSGDEFVVVLTQYDTDHLTEVVKRIQERLAMPCHIN 672 GDELLRTVATRLQSQARRSDIVGRLSGDEFVVVLTQYDT HLTEVVKRIQERLAMPCHIN Sbjct: 612 GDELLRTVATRLQSQARRSDIVGRLSGDEFVVVLTQYDTQHLTEVVKRIQERLAMPCHIN 671 Query: 673 GTPLNPSASIGVAIFPDNGHDIETLLHRADIAMYQAKAVNRGRFSFFSEEMNSLAQERLV 732 GTPLNPSASIGVAIFPDNG+DIETLLHRADIAMYQAKAVNRGRFSFFSEEMNS+AQERL Sbjct: 672 GTPLNPSASIGVAIFPDNGNDIETLLHRADIAMYQAKAVNRGRFSFFSEEMNSMAQERLA 731 Query: 733 LEGALRDALRGGSFELHFQPQINLHDLSLHGVEALARWYDPQLGQISPGRFIPIAEECGL 792 LE ALRDALRGG EL+FQPQI LHD SLHGVEALARW+ PQLGQISPGRFIPIAEECGL Sbjct: 732 LESALRDALRGGDLELNFQPQIKLHDQSLHGVEALARWHHPQLGQISPGRFIPIAEECGL 791 Query: 793 IGELGKWALEEACFQLADWRRRGIQVPSVSVNLSATNFHDLDLPQFLTGLLRRYSLAPTD 852 IGELGKWALEEACFQL+DWRRRGIQVPS+SVNLSATNFHDLDLPQF+TGLLRRY+L P+D Sbjct: 792 IGELGKWALEEACFQLSDWRRRGIQVPSISVNLSATNFHDLDLPQFVTGLLRRYALEPSD 851 Query: 853 LTLEITETVLMDTNPSTNQTIAAVHALGIPLAMDDFGTGYSSLGYLRKLPVSELKLDQSF 912 LTLEITETVLMDTNPST+QTIAAVHALGIPLAMDDFGTGYSSLGYLRKLPVSELKLDQSF Sbjct: 852 LTLEITETVLMDTNPSTSQTIAAVHALGIPLAMDDFGTGYSSLGYLRKLPVSELKLDQSF 911 Query: 913 VRDMAKDEAVSALTNAVIRIGESLKLTVVAEGVEDADQYRLLISQGCDVAQGYYFSRPMS 972 VRDMAKD AVSALTNAVIRIGESLKLTVVAEGVEDA+QY LLISQGCDVAQGY FSRPMS Sbjct: 912 VRDMAKDPAVSALTNAVIRIGESLKLTVVAEGVEDAEQYALLISQGCDVAQGYLFSRPMS 971 Query: 973 ADQLESWVRDYRGENAAPPGATGP 996 AD LE+WVRD +P G P Sbjct: 972 ADALENWVRDLHA-GGSPDGRWAP 994 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 372 bits (955), Expect = e-104, Method: Compositional matrix adjust. Identities = 199/424 (46%), Positives = 266/424 (62%), Gaps = 1/424 (0%) Query: 562 DSLTGLPNRSLLLVQAGQAIAAAERNRSMVAVMFIDLDRFKQVNDSLGHQAGDELLRTVA 621 D+LTGL NR +L QAI AER + + V+F+DLDRFK VND+LGH+AGD LLRTVA Sbjct: 522 DTLTGLINRHMLRHHLEQAIGVAERQKQALWVVFVDLDRFKFVNDTLGHKAGDTLLRTVA 581 Query: 622 TRLQSQARRSDIVGRLSGDEFVVVLTQYDTDHL-TEVVKRIQERLAMPCHINGTPLNPSA 680 RL++ R +D V RL GDEF+++L + D L T ++RI + +A P + G L + Sbjct: 582 GRLRAAVREADTVARLGGDEFIMILPEQDELTLDTRSLQRIMDAVAAPVTLGGHTLFMTC 641 Query: 681 SIGVAIFPDNGHDIETLLHRADIAMYQAKAVNRGRFSFFSEEMNSLAQERLVLEGALRDA 740 S+GVA +P++G D ETL+ ADIAMY+ K R F F++ MN A ERL +EG LR+A Sbjct: 642 SVGVACYPNDGTDGETLIKHADIAMYRGKQTGRNNFQFYTPAMNEQALERLRIEGDLRNA 701 Query: 741 LRGGSFELHFQPQINLHDLSLHGVEALARWYDPQLGQISPGRFIPIAEECGLIGELGKWA 800 L G F LH+QP+++L + G EAL RW PQLG +SP RFI +AEE GLI +G W Sbjct: 702 LDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQLGMVSPARFIGLAEETGLILPIGAWV 761 Query: 801 LEEACFQLADWRRRGIQVPSVSVNLSATNFHDLDLPQFLTGLLRRYSLAPTDLTLEITET 860 L AC Q W+ G SVSVNLS F DL + +L L P L LE+TE+ Sbjct: 762 LRAACTQCRQWQLGGYADISVSVNLSVGQFLQKDLVSSIADVLEDTGLPPESLELELTES 821 Query: 861 VLMDTNPSTNQTIAAVHALGIPLAMDDFGTGYSSLGYLRKLPVSELKLDQSFVRDMAKDE 920 ++M +A + LG+ L++DDFGTGYSSL YL++ P+ LK+D+SFVRD+ D Sbjct: 822 LMMTDVEHAIGILAGLKKLGVRLSIDDFGTGYSSLAYLKRFPIDVLKIDRSFVRDITIDA 881 Query: 921 AVSALTNAVIRIGESLKLTVVAEGVEDADQYRLLISQGCDVAQGYYFSRPMSADQLESWV 980 +A+T ++I + LKL V+AEGVE +Q L GCD QGY FS P+ AD+ E + Sbjct: 882 DDAAITASIISLARHLKLRVIAEGVETDEQLDYLRHYGCDEIQGYLFSPPVPADKFEQIL 941 Query: 981 RDYR 984 R R Sbjct: 942 RQDR 945 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 371 bits (953), Expect = e-103, Method: Compositional matrix adjust. Identities = 196/438 (44%), Positives = 271/438 (61%), Gaps = 3/438 (0%) Query: 547 EREASRARIHQLAFYDSLTGLPNRSLLLVQAGQAIAAAERNRSMVAVMFIDLDRFKQVND 606 ER+A A + A +D+LTGLPNR++L +A+ A + ++ AV F+DLD+FK +ND Sbjct: 254 ERKAHEAEMRHQATHDALTGLPNRNMLHEHLQRALRVARQKGALTAVAFVDLDQFKFIND 313 Query: 607 SLGHQAGDELLRTVATRLQSQARRSDIVGRLSGDEFVVVLTQYDTDH--LTEVVKRIQER 664 SLGHQ GDELL+TVA RLQ+ R +D+V R GDEFV+VL + EV++RI Sbjct: 314 SLGHQVGDELLKTVAQRLQACLREADMVARQGGDEFVLVLQNQTEGEAGIAEVMQRILAA 373 Query: 665 LAMPCHINGTPLNPSASIGVAIFPDNGHDIETLLHRADIAMYQAKAVNRGRFSFFSEEMN 724 +A P +ASIGV+ +P +G D+E+LL AD AMY+AK R F FF+ M+ Sbjct: 374 VARPWQAGDREFQVTASIGVSRYPADGKDVESLLKHADSAMYRAKEQGRNNFQFFAPWMD 433 Query: 725 SLAQERLVLEGALRDALRGGSFELHFQPQINLHDLSLHGVEALARWYDPQLGQISPGRFI 784 S RL + LR AL F+L++QP+++L D L G EAL RW PQ G + P RFI Sbjct: 434 SQVSNRLEMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWESPQQGMVPPDRFI 493 Query: 785 PIAEECGLIGELGKWALEEACFQLADWRRRGIQVPSVSVNLSATNFHDLDLPQFLTGLLR 844 P AEE GLI +G+W L AC Q W++ G+ V+VNLS + LP F+ G+L Sbjct: 494 PFAEEAGLIVPIGEWVLRTACRQNQAWQQAGLPPIPVAVNLSPRQLNQ-SLPDFVAGVLA 552 Query: 845 RYSLAPTDLTLEITETVLMDTNPSTNQTIAAVHALGIPLAMDDFGTGYSSLGYLRKLPVS 904 + LA + L LEITE V+M + T+ A+ LG+ +++DDFGTGYSSL YLR+ PV Sbjct: 553 QSGLAASWLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSLSYLRRFPVD 612 Query: 905 ELKLDQSFVRDMAKDEAVSALTNAVIRIGESLKLTVVAEGVEDADQYRLLISQGCDVAQG 964 LK+D+SFVRD+ +D +A+ A+I + L L V+AEGVED +Q+ L CD QG Sbjct: 613 ALKIDKSFVRDIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQHAFLKENACDEVQG 672 Query: 965 YYFSRPMSADQLESWVRD 982 YYF +PM +W+ + Sbjct: 673 YYFGKPMEVAVFTAWMSE 690 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 367 bits (941), Expect = e-102, Method: Compositional matrix adjust. Identities = 221/652 (33%), Positives = 345/652 (52%), Gaps = 16/652 (2%) Query: 354 EQPVWISAVVNPILDVQGKLINA--------VCVMTDITNTKVHEVLQY---RVLNAMAR 402 +Q W++ N I+ L A ++ DI+ K E+LQ RVL+ + Sbjct: 453 QQFEWVTVRRNKIIHTDVTLNRAEFERGYAIFAILRDISARKRAELLQSEQNRVLHMVMA 512 Query: 403 DSSLAEQMNLLCEEVERVAPEVIASVLRVDEQG-RLHTLAAPSLPEAYTRAIEGIQIGPL 461 D L + + + P SV+ V+EQ ++ + P RA++G+ I Sbjct: 513 DDELDGVLYEIVNFADAQIPYGACSVMLVNEQASHFTSVLSHRYPPRLIRALQGMPIRHG 572 Query: 462 VGSCGSAAYTGESVVSEEIATDPHWADYKEIALSHGLQACWSTPIKSADGKVIGTFGFYY 521 G AA + IA DP + + + +C + PI +G+++G F Sbjct: 573 CGVGAEAALRRGPCIVANIAQDPITEHLRPLIDTEIYPSCGAWPIIGKEGQLLGVFAALL 632 Query: 522 RQPRAPSAFHHLLVDAGLHLCALAIEREASRARIHQLAFYDSLTGLPNRSLLLVQAGQAI 581 ++ +APS + L + L ++AIE + RI LA YD LTGLPNR L AI Sbjct: 633 KENQAPSNEYLQLANIAADLASVAIESRRADERIRHLAHYDELTGLPNRFLCTQHVSNAI 692 Query: 582 AAAERNRSMVAVMFIDLDRFKQVNDSLGHQAGDELLRTVATRLQSQARRSDIVGRLSGDE 641 AE MVAV+ +DLDRFK +ND+ GH+ G+ +L+ +A R ++ R DI+ R+ GDE Sbjct: 693 THAEHRNGMVAVILMDLDRFKNINDTFGHETGEAVLQEIAQRFRNSLRELDILARVGGDE 752 Query: 642 FVVVLTQYDTD-HLTEVVKRIQERLAMPCHINGTPLNPSASIGVAIFPDNGHDIETLLHR 700 F+V++ +D L E+ +++ P I+G S SIG+A +P +G + +TL+ Sbjct: 753 FIVLVDDFDDPVQLGEIAQKLLTEARKPFIIDGQEAMLSVSIGIATYPGDGDNAQTLIKN 812 Query: 701 ADIAMYQAKAVNRGRFSFFSEEMNSLAQERLVLEGALRDALRGGSFELHFQPQINLHDLS 760 ADIAMY+AK + + FFS+E+N+ ER+ LE LR A+ F +H+QP+I L + Sbjct: 813 ADIAMYRAKHHGKDDYRFFSDEINTNTVERIALEAELRRAIERAEFVVHYQPKIELAEGR 872 Query: 761 LHGVEALARWYDPQLGQISPGRFIPIAEECGLIGELGKWALEEACFQLADWRRRGIQVPS 820 + G EAL RW P G + PG FI +AEE LI ++G L+ AC + R++ + Sbjct: 873 IVGAEALVRWQHPLRGLLPPGEFITLAEETRLIDQIGLLVLDVACRDIGLMRQQQMTCGR 932 Query: 821 VSVNLSATNFHDLDLPQFLTGLLRRYSLAPTDLTLEITETVLMDTNPSTNQTIAAVHALG 880 +S+NL+ + F D L + ++ R+ P+ L EITE+++M + + A G Sbjct: 933 ISINLTGSQFGDERLLDDIKEVVDRHGTPPSCLEFEITESMVMHNRERAIAIMDGIRAEG 992 Query: 881 IPLAMDDFGTGYSSLGYLRKLPVSELKLDQSFVRDMAKDEAVSALTNAVIRIGESLKLTV 940 +++DDFGTGYSSL YL++ PV+ LK+D+SF+ D+ + SA+ A+I + +L L V Sbjct: 993 FTISIDDFGTGYSSLAYLKRFPVNTLKIDKSFINDIPDNPNSSAIVQAIIAMSHALNLKV 1052 Query: 941 VAEGVEDADQYRLLISQGCDVAQGYYFSRPMSADQLESWVRDYRGENAAPPG 992 V EGVE Q + L G D QGYYFS+P+ Q +V+ G+ P G Sbjct: 1053 VTEGVETEKQLQALREFGSDEYQGYYFSKPVPYAQ---FVQLLAGQQDRPQG 1101 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 343 bits (881), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 180/444 (40%), Positives = 261/444 (58%), Gaps = 8/444 (1%) Query: 547 EREASRARIHQLAFYDSLTGLPNRSLLLVQAGQAIAAAERNRSMVAVMFIDLDRFKQVND 606 +R+ + RI +LAF+D+LT LPNR+LLL + QAIA + R ++ A++FIDLD FK +ND Sbjct: 587 DRKLAEERIRELAFFDALTRLPNRTLLLDRLKQAIALSLRTKTCAALLFIDLDHFKTLND 646 Query: 607 SLGHQAGDELLRTVATRLQSQARRSDIVGRLSGDEFVVVL------TQYDTDHLTEVVKR 660 ++GH+ GD LL+ VA RL + R +D V R+ GDEFVVVL Q + ++ Sbjct: 647 TIGHEKGDTLLKQVAQRLLANVRENDTVARVGGDEFVVVLEGLNELQQEAATQTRAIGEK 706 Query: 661 IQERLAMPCHINGTPLNPSASIGVAIFPDNGHDIETLLHRADIAMYQAKAVNRGRFSFFS 720 I L ++ +ASIG +F ++ L+ +AD+AMY+AK R FF Sbjct: 707 ILTALGSTYQLDDVEYRTTASIGATVFLGRAASVDELMKQADLAMYKAKETGRNALRFFD 766 Query: 721 EEMNSLAQERLVLEGALRDALRGGSFELHFQPQINLHDLSLHGVEALARWYDPQLGQISP 780 M + ER LE LR A+ G LH+QPQ+ + + G E L RW PQ G + P Sbjct: 767 PAMQTAVLERATLEAGLRKAIDGNQLLLHYQPQV-VEGGRVTGAEVLVRWQHPQRGMVPP 825 Query: 781 GRFIPIAEECGLIGELGKWALEEACFQLADWRRRGIQVP-SVSVNLSATNFHDLDLPQFL 839 G FIP+AEE GLI LG W LE AC QLA W + S++VN+SA + D Q + Sbjct: 826 GDFIPLAEETGLILSLGNWVLETACRQLATWAAQPQMADLSIAVNVSAPQLREPDFVQTV 885 Query: 840 TGLLRRYSLAPTDLTLEITETVLMDTNPSTNQTIAAVHALGIPLAMDDFGTGYSSLGYLR 899 + R P L LE+TE++L+D Q + A+ A G+ ++DDFGTGYSSL YL+ Sbjct: 886 LDTIERTGANPRRLKLELTESLLVDNVEDIIQKMFALKAYGVEFSLDDFGTGYSSLSYLK 945 Query: 900 KLPVSELKLDQSFVRDMAKDEAVSALTNAVIRIGESLKLTVVAEGVEDADQYRLLISQGC 959 +LP+++LK+DQSFVRD+ D +++ ++ + +SL L V+AEGVE Q L GC Sbjct: 946 RLPLNQLKIDQSFVRDVLIDPNDASIAKTIVNLAQSLGLGVIAEGVETDAQRSFLADAGC 1005 Query: 960 DVAQGYYFSRPMSADQLESWVRDY 983 QGY+F RP++ + E + +++ Sbjct: 1006 HAYQGYFFCRPVTVEAFEKFAQEF 1029 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 3,058,649 Number of extensions: 126649 Number of successful extensions: 524 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 454 Number of HSP's successfully gapped: 45 Length of query: 998 Length of database: 1,619,870 Length adjustment: 95 Effective length of query: 903 Effective length of database: 1,170,045 Effective search space: 1056550635 Effective search space used: 1056550635 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 135 (56.6 bits)