BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0051.p (442 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310169|ref|YP_003774261.1| methyl-accepting chemotaxis tra... 760 0.0 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 114 2e-26 gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis tra... 108 8e-25 gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 105 1e-23 gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis tra... 103 2e-23 >gi|300310169|ref|YP_003774261.1| methyl-accepting chemotaxis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 657 Score = 760 bits (1963), Expect = 0.0, Method: Compositional matrix adjust. Identities = 360/422 (85%), Positives = 394/422 (93%) Query: 1 MTVALVGIVFVIALMLITYSASRMVESRSIEQMGNDARGVANMVGIFDRSVNSQVDRFST 60 MTVALVGIVFVIAL+LITYSASRM+E RS+EQM ND RG+ANMVG+FDRSVNSQVDRFST Sbjct: 25 MTVALVGIVFVIALLLITYSASRMIEQRSVEQMRNDTRGIANMVGLFDRSVNSQVDRFST 84 Query: 61 MFDKEFGKLTLDESRTVQIGDRATPVLSNDGRELNLDFSIPDRFTAHTGVTATIFAKTGD 120 MFD EFGKL+LDE+RTVQIG+RATPVL NDG ELNLDFSIPDRFTA TGVTATIFAKTG+ Sbjct: 85 MFDSEFGKLSLDETRTVQIGERATPVLQNDGHELNLDFSIPDRFTAKTGVTATIFAKTGE 144 Query: 121 DFVRVSTSVKKENGERAVGTLLDRAHPGYAALKSDKTYSGVATLFGKQYITKYVPFKNDA 180 DFVR+STSVKKENGERA+GTLLDR+HPGYAALKSDK YSG+ATLFGKQYITKYVP KN+A Sbjct: 145 DFVRISTSVKKENGERAIGTLLDRSHPGYAALKSDKAYSGLATLFGKQYITKYVPVKNNA 204 Query: 181 GQIIAALYVGIDISADVKALKDQIKSLKIAETGNFYALNGREGKDQGVMIAGAPREGKEE 240 GQIIAALYVGIDIS+D+KALKDQIK+LKI +TGNF+ALN REGKD GVMIAGA REGKEE Sbjct: 205 GQIIAALYVGIDISSDIKALKDQIKALKIGDTGNFFALNAREGKDLGVMIAGAAREGKEE 264 Query: 241 GGNLIEAKDAGGNTYIKTLVQQKEGQLRYTGSDGRQKIAVFTHYAPWNWVIVGEAAVDEI 300 G +L+E KDA G+TYIKT++QQK+G L YT +DGR+KI FT+Y W+WVI GEAAVDEI Sbjct: 265 GSSLLELKDAAGDTYIKTMLQQKDGSLPYTAADGRKKILAFTYYPGWHWVIAGEAAVDEI 324 Query: 301 TREVNSMRNLYIGLGLLFLAVLGAILTVMINRMITRPLDAVCNAADRIAGGDLTTRLHVH 360 TREV+SMRNLYIGLGLLFLAVL AILTVM+NRM++RPLDAVC AADRIA GDLTTRLHVH Sbjct: 325 TREVSSMRNLYIGLGLLFLAVLAAILTVMVNRMVSRPLDAVCQAADRIASGDLTTRLHVH 384 Query: 361 SSDEIGRLMGAFNGISMGLSEVVSSVRAGTEEINTAASEIAAGNLNLSSRTEQQAGALEE 420 SSDEIGRLMGAFNGISMGLSEVVSSVRAGTEEINTAASEIA+GNLNLS RTEQQAGALEE Sbjct: 385 SSDEIGRLMGAFNGISMGLSEVVSSVRAGTEEINTAASEIASGNLNLSVRTEQQAGALEE 444 Query: 421 TA 422 TA Sbjct: 445 TA 446 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 114 bits (285), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 63/140 (45%), Positives = 90/140 (64%), Gaps = 2/140 (1%) Query: 283 HYAPWNWVIVGEAAVDEITREVNSMRNLYIGLGLLFLAVLGAILTVMINRMITRPLDAVC 342 YA + ++ + A I R+ S R++ + LG + + VLGA L ++ R ITRPL+ Sbjct: 163 RYADFQKQVIDQTAA-AINRDYRSGRSMLLALGAIEI-VLGAALAWLLTRSITRPLNQAV 220 Query: 343 NAADRIAGGDLTTRLHVHSSDEIGRLMGAFNGISMGLSEVVSSVRAGTEEINTAASEIAA 402 A+ +A GDLT+R+ S DE G+L+ A ++ L ++V VR GTE INTA+ EIA Sbjct: 221 TIAETVAAGDLTSRIDSSSRDETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIAT 280 Query: 403 GNLNLSSRTEQQAGALEETA 422 GNL+LS+RTE QAG+LEETA Sbjct: 281 GNLDLSARTEAQAGSLEETA 300 >gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 108 bits (270), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 59/115 (51%), Positives = 76/115 (66%), Gaps = 1/115 (0%) Query: 310 LYIGLGLLFLAVLGAI-LTVMINRMITRPLDAVCNAADRIAGGDLTTRLHVHSSDEIGRL 368 + + L AVL AI L ++ R I +PL A+ +A GDLTTR+ HS DE G+L Sbjct: 192 IEVTLACSMAAVLAAIILGYLMARSIVKPLTEAVRVAENVAAGDLTTRIEPHSRDETGQL 251 Query: 369 MGAFNGISMGLSEVVSSVRAGTEEINTAASEIAAGNLNLSSRTEQQAGALEETAG 423 M A ++ L E+VS VR GTE I TA+ EIA+GNL+LS+RTEQQAG+LEETA Sbjct: 252 MQALRKMNDNLIEIVSGVRRGTESITTASGEIASGNLDLSARTEQQAGSLEETAS 306 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 105 bits (261), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 58/127 (45%), Positives = 83/127 (65%), Gaps = 2/127 (1%) Query: 298 DEITREVNSMRNLYIGLGLLFLAVLGAI-LTVMINRMITRPLDAVCNAADRIAGGDLTTR 356 D++ +V L+I +GLL V+ A+ L VM+ R ++RPL+ A+R+A GDLT Sbjct: 179 DDMAEQVYRSATLWI-IGLLAAIVVIALGLAVMVARAVSRPLNEAVAVAERVAQGDLTMD 237 Query: 357 LHVHSSDEIGRLMGAFNGISMGLSEVVSSVRAGTEEINTAASEIAAGNLNLSSRTEQQAG 416 + S+DE G LM + ++ L ++V VR GT+ I TA+ EIA GNL+LSSRTE+QAG Sbjct: 238 IRATSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGNLDLSSRTEEQAG 297 Query: 417 ALEETAG 423 +LEETA Sbjct: 298 SLEETAS 304 >gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 579 Score = 103 bits (258), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 74/191 (38%), Positives = 102/191 (53%), Gaps = 28/191 (14%) Query: 235 REGK-EEGGNLIEAKDAGGNTYIKTLVQQ--KEGQLRYTGSDGRQKIAVFTHYAPWNWVI 291 R+GK EE NL + +T +QQ K + G+D K A + W+I Sbjct: 138 RQGKDEEARNL---QRGESTKAYRTSLQQIDKLAAVNDKGADISNKQADAIYAQARLWII 194 Query: 292 VGEAAVDEITREVNSMRNLYIGLGLLFLAVLGAILTVMINRMITRPLDAVCNAADRIAGG 351 V AA IGL +L L V ++R+I RPL+ + A R+AGG Sbjct: 195 VTVAAC--------------IGLAML--------LAVWVSRLIARPLEEAVSVAQRVAGG 232 Query: 352 DLTTRLHVHSSDEIGRLMGAFNGISMGLSEVVSSVRAGTEEINTAASEIAAGNLNLSSRT 411 DLTTR+ DE G+L+ A ++ L +V VR GT+ I TA++EIA+GNL+LSSRT Sbjct: 233 DLTTRIEPAGKDETGQLLVALKSMNDSLVGIVGQVRMGTQTITTASTEIASGNLDLSSRT 292 Query: 412 EQQAGALEETA 422 E+QA +LEETA Sbjct: 293 EEQASSLEETA 303 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,252,224 Number of extensions: 51582 Number of successful extensions: 301 Number of sequences better than 1.0e-08: 58 Number of HSP's gapped: 292 Number of HSP's successfully gapped: 58 Length of query: 442 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 354 Effective length of database: 1,203,190 Effective search space: 425929260 Effective search space used: 425929260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)