BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0045.p (282 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310066|ref|YP_003774158.1| hypothetical protein Hsero_0731... 385 e-108 >gi|300310066|ref|YP_003774158.1| hypothetical protein Hsero_0731 [Herbaspirillum seropedicae SmR1] Length = 281 Score = 385 bits (988), Expect = e-108, Method: Compositional matrix adjust. Identities = 179/253 (70%), Positives = 212/253 (83%) Query: 30 DQITWGKYNVPPYMIRSGEFAHQGIFDLTLNVLKKEMPGYEHVELEAPFPRINSEIRKGS 89 +QI WG+++VPPYMIR GE+A +GIFDLTL V+ +P Y+HV+L APFPR+ SEIRKGS Sbjct: 29 NQIIWGRHHVPPYMIREGEWAGRGIFDLTLGVVTSHLPQYQHVDLAAPFPRVISEIRKGS 88 Query: 90 HWCFNGMLKTPEREHYGYLSLPMSIFLPLRIIVRRDRVEEFRHRTSLTGLLQDPRLTTSV 149 HWC+ G L+T ERE Y Y SLP SIFLPLR+IVRRDR+ +F H SL LLQ+ +L TSV Sbjct: 89 HWCYIGALRTSEREAYAYFSLPTSIFLPLRVIVRRDRLAQFSHPQSLKSLLQNRQLVTSV 148 Query: 150 MRDRSYSPTVDKLLATYPPRENYSEQMEAIGMLLAGRLDYMVELPLLAFDQARAMGHAGE 209 MRDRSYSPTVD+LLA YP RENYSEQ+EAI MLLAGR+DYM+ELPLLAF QAR +GHA E Sbjct: 149 MRDRSYSPTVDRLLAEYPARENYSEQIEAISMLLAGRIDYMIELPLLAFYQARLLGHADE 208 Query: 210 LVALPMREANEVVFNRVMCPHNEWGRQVVEQVNKVLLAERAKPSYRAIVEKWHDPESVAE 269 L A+ EA+EVVFNRVMCP N WGRQV++QVN+VLLAER P+YRA+VE+WHDPES AE Sbjct: 209 LAAVRTEEADEVVFNRVMCPRNAWGRQVIDQVNQVLLAERPTPAYRAMVERWHDPESTAE 268 Query: 270 IRKIYDKVFLKIP 282 IR++YDKVFLK P Sbjct: 269 IRRLYDKVFLKTP 281 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.138 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 820,965 Number of extensions: 30968 Number of successful extensions: 101 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 101 Number of HSP's successfully gapped: 1 Length of query: 282 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 198 Effective length of database: 1,222,130 Effective search space: 241981740 Effective search space used: 241981740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 130 (54.7 bits)