BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0032.p (547 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310055|ref|YP_003774147.1| methyl-accepting chemotaxis tra... 853 0.0 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 423 e-119 gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis pro... 400 e-112 gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis pro... 395 e-111 gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 394 e-111 >gi|300310055|ref|YP_003774147.1| methyl-accepting chemotaxis transducer transmembrane protein I [Herbaspirillum seropedicae SmR1] Length = 547 Score = 853 bits (2205), Expect = 0.0, Method: Compositional matrix adjust. Identities = 465/547 (85%), Positives = 500/547 (91%) Query: 1 MKNLKIGHRLGLGFAAVLVLLVAVATFGLQQVSRLNDRIVFLTSVDEGKLEALSKVQFAV 60 MKNLKIGHRLGLGFAAVL+LLVAVA FGLQQVSRLNDRIVFLTSVDEGKLEALSKVQFAV Sbjct: 1 MKNLKIGHRLGLGFAAVLILLVAVAAFGLQQVSRLNDRIVFLTSVDEGKLEALSKVQFAV 60 Query: 61 GLRAIAARNLTLVNGAAEQKGDLDLVGSAQKDIESGMAELTRLMQDPATTPQERDMLEQL 120 GLRAIAARNLTLV AA+QK DLDLVGS+Q+DI++GMAEL RLMQ PA++PQER+MLE+L Sbjct: 61 GLRAIAARNLTLVQSAADQKNDLDLVGSSQQDIDAGMAELGRLMQHPASSPQEREMLEKL 120 Query: 121 RKLEAQYLPVAVNIVKLATSQQNEAARTSLVKDCMPILNRVIAHVEQFNKLLRKNSAANV 180 R+LEAQYLPVAVNIVKLATSQQ EAAR SLVKDCMPILNRVIA VEQFNKLLRKNS ANV Sbjct: 121 RQLEAQYLPVAVNIVKLATSQQTEAARASLVKDCMPILNRVIAQVEQFNKLLRKNSEANV 180 Query: 181 DAAEAAYAMAKWTMMGASMAALVLGGLIAWLLTRSISRPLAQAVSVAQSVQSGDLGSRIE 240 + AEAAY +AKWTM+ AS+AAL LGG++AWLLTRSISRPLAQAVSVAQSVQSGDLGSRIE Sbjct: 181 EEAEAAYTLAKWTMLAASLAALTLGGMVAWLLTRSISRPLAQAVSVAQSVQSGDLGSRIE 240 Query: 241 VTSKDELGQLMAALKGMNDSLVAIVGEVRHGTDTIAVASDEIKRGNMDLSHRTEQQAGSL 300 VTSKDELGQLM+ALKGMNDSLV IVGEVR GTDTIAVASDEIK+GNMDLS+RTEQQAGSL Sbjct: 241 VTSKDELGQLMSALKGMNDSLVNIVGEVRQGTDTIAVASDEIKQGNMDLSNRTEQQAGSL 300 Query: 301 QETASAMEQLTATVKQTSDNAREAKRLADNASDVATKGGAAVNRVVETMHAISTSSHKIV 360 QETASAMEQLTATVKQ +DNAREA RLADNAS VA +GGAAV RVV+TM++IS SSHKIV Sbjct: 301 QETASAMEQLTATVKQNADNAREANRLADNASQVAARGGAAVGRVVDTMNSISASSHKIV 360 Query: 361 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAVAAKEIEALIN 420 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSAVAAKEIEALIN Sbjct: 361 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSAVAAKEIEALIN 420 Query: 421 ESVAKVATGEKQVSEAGSTMDEVVRSIQSVTHIVQDISAASTEQTAGLEQINTAVARIDE 480 ESVAKV TGEKQVSEAGSTM EVV SI+SVTHI+ DISAAS EQT+GLEQIN AVA+IDE Sbjct: 421 ESVAKVVTGEKQVSEAGSTMHEVVESIKSVTHIMHDISAASAEQTSGLEQINRAVAQIDE 480 Query: 481 TTQQNAALVEQAAAAAASMQQQSVALTDVVSIFKLTAEQAGPTAVRGPQQAAVQYSQDTA 540 TQQNAALVEQAAAAAASMQQQSVALT+VVSIFKL+ Q P +R ++ S Sbjct: 481 ATQQNAALVEQAAAAAASMQQQSVALTEVVSIFKLSRAQNAPQHLRSAEREDAAVSGPVH 540 Query: 541 MPALSYA 547 M ALSYA Sbjct: 541 MEALSYA 547 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 423 bits (1087), Expect = e-119, Method: Compositional matrix adjust. Identities = 269/523 (51%), Positives = 354/523 (67%), Gaps = 20/523 (3%) Query: 1 MKNLKIGHRLGLGFAAVLVLLVAVATFGLQQVSRLNDRIVFLTSVDEGKLEALSKVQFAV 60 MKNLKIG RL +GF A+L+L+ + G+ ++ + D +T + AL K + AV Sbjct: 1 MKNLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRI------ALKKERDAV 54 Query: 61 GLRAIAARNLTLVNGAAEQKGDLD-LVGSAQKDIESGMAELTRLMQD---PATTPQERDM 116 A N V A K D D QK I++ A +++L ++ T +E+ + Sbjct: 55 QWHAAIKENG--VRTFAVMKSDDDAFQAYFQKQIDAQSARISQLQKEVEAAITDAEEKRL 112 Query: 117 LEQLRKLEAQYLPVAVNIVKLATSQQNEAAR----TSLVKDCMPILNRVIAHVEQFNKLL 172 +++ KL A Y +I + T+ + AR T+LVK + V+ + + F K + Sbjct: 113 FDEVGKLRAAYRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLRYAD-FQKQV 171 Query: 173 RKNSAANVDAAEAAYAMAKWTMMGASMAALVLGGLIAWLLTRSISRPLAQAVSVAQSVQS 232 +AA ++ Y + ++ +VLG +AWLLTRSI+RPL QAV++A++V + Sbjct: 172 IDQTAAAINRD---YRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAA 228 Query: 233 GDLGSRIEVTSKDELGQLMAALKGMNDSLVAIVGEVRHGTDTIAVASDEIKRGNMDLSHR 292 GDL SRI+ +S+DE G+L+AALK MN +L+ IVG VR GT+TI AS EI GN+DLS R Sbjct: 229 GDLTSRIDSSSRDETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSAR 288 Query: 293 TEQQAGSLQETASAMEQLTATVKQTSDNAREAKRLADNASDVATKGGAAVNRVVETMHAI 352 TE QAGSL+ETASAMEQLT+TVKQ +DNAR+A LA +AS VAT+GGA V++VV TM AI Sbjct: 289 TEAQAGSLEETASAMEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAI 348 Query: 353 STSSHKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAVAA 412 SS KI DII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA AA Sbjct: 349 HASSQKIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAA 408 Query: 413 KEIEALINESVAKVATGEKQVSEAGSTMDEVVRSIQSVTHIVQDISAASTEQTAGLEQIN 472 KEI+ LI++SV KV G + V+ AG TM EVV+S+ VT +V++I+ AS EQ+ G+EQ+N Sbjct: 409 KEIKQLIDDSVEKVDDGSRLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIEQVN 468 Query: 473 TAVARIDETTQQNAALVEQAAAAAASMQQQSVALTDVVSIFKL 515 A+ IDETTQQNAALVEQAAAAA SMQ Q+ +LTD VS+F+L Sbjct: 469 LAITHIDETTQQNAALVEQAAAAARSMQDQAASLTDAVSVFRL 511 >gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 583 Score = 400 bits (1028), Expect = e-112, Method: Compositional matrix adjust. Identities = 247/536 (46%), Positives = 356/536 (66%), Gaps = 15/536 (2%) Query: 1 MKNLKIGHRLGLGFAAVLVLLVAVATFGLQQVSRLNDRIVFLTSVDEGKLEALSKVQFAV 60 NL I RL + F+ +++++ + GL +++ +N + + + K+ ++ + V Sbjct: 3 FNNLSIATRLAIAFSILILVIAGIVALGLARLADINKALDLVVNDRYKKIAIINTISGKV 62 Query: 61 GLRAIAARNLTLVNGAAEQKGDLDLVGSAQKDIESGMAELTRLMQDPA---TTPQERDML 117 AI+ RN L+ A+ + + QK IES AE T+L + P+ + L Sbjct: 63 DDVAISLRNQLLMTDPAQ-------IAAEQKTIESLSAENTKLYDELTRLVANPEAKAKL 115 Query: 118 EQLRKLEAQYLPVAVNIVKLATSQQNEAARTSLVKDCMPILNRVIAHVEQFNKLLRKNSA 177 +++ + Y + I KL +S +AA ++ + +P+ + +++ + +K Sbjct: 116 DKVTETRKSYAALRKQIDKLQSSGDKDAAIKLMLSEMVPLQKIYFSQLDELSDTQQKLMD 175 Query: 178 ANVDAAEAAYAMAKWTMMGASMAALVLGGLIAWLLTRSISRPLAQAVSVAQSVQSGDLGS 237 +VD AEAAY + M+ A + A IAW ++RSI+RPL++AV VA++V +GDL + Sbjct: 176 QSVDQAEAAYETTRMIMVIAGVLAATFAAFIAWTISRSITRPLSRAVEVAETVAAGDLTA 235 Query: 238 RIEVTSKDELGQLMAALKGMNDSLVAIVGEVRHGTDTIAVASDEIKRGNMDLSHRTEQQA 297 I S DE G+L+ AL MN L AIV EVR GTD++ AS +I GN+DLS RTE+QA Sbjct: 236 VIAAHSNDETGRLLQALAAMNQKLKAIVLEVRRGTDSMVTASTQIATGNLDLSSRTEEQA 295 Query: 298 GSLQETASAMEQLTATVKQTSDNAREAKRLADNASDVATKGGAAVNRVVETMHAISTSSH 357 +L+ETAS++E+LT++VKQ +D+ R++ LA NAS VA +GG AV +VV+TM AI+ SSH Sbjct: 296 SALEETASSLEELTSSVKQNADHTRKSSELAANASQVANQGGLAVKQVVQTMGAINDSSH 355 Query: 358 KIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAVAAKEIEA 417 KIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR LAQRSA AAKEI+ Sbjct: 356 KIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRGLAQRSATAAKEIKE 415 Query: 418 LINESVAKVATGEKQVSEAGSTMDEVVRSIQSVTHIVQDISAASTEQTAGLEQINTAVAR 477 LIN+SV +V +G + V++AGSTMD VV SI+ V+HIV +ISAA+ EQ+ G+EQ+N A+ + Sbjct: 416 LINDSVNQVTSGTELVAQAGSTMDAVVNSIEQVSHIVGEISAATREQSDGIEQVNQAIMQ 475 Query: 478 IDETTQQNAALVEQAAAAAASMQQQSVALTDVVSIFKLTAEQAGPTAVRGPQQAAV 533 +D TTQQNAALVE+AAAAA ++Q Q+ L D+V +F+L ++ G + GP +AV Sbjct: 476 MDNTTQQNAALVEEAAAAAQALQDQAQRLLDLVGVFRL--DEVGGS---GPAPSAV 526 >gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 576 Score = 395 bits (1016), Expect = e-111, Method: Compositional matrix adjust. Identities = 267/553 (48%), Positives = 365/553 (66%), Gaps = 21/553 (3%) Query: 1 MKNLKIGHRLGLGFAAVLVLLVAVATFGLQQ---VSRLNDRIVFLTSVDEGKLEALSKVQ 57 + ++KI RLG FAA++V++ +A G++ VS D IV V K+ +++ Sbjct: 4 ISDMKISVRLGACFAALIVVMCVIAGVGVKNLLMVSDATDEIVNDRYV---KVALGTQIT 60 Query: 58 FAVGLRAIAARNLTLVNGAAEQKGDLDLVGSAQKDIESGMAELTRLMQDPATTPQERDML 117 V A + RN + EQK LD + + + +AE M+ TP+ ++L Sbjct: 61 DKVNTGARSLRNAIMARNPEEQKKYLDRMDTYTSQVSEHLAE----MEKRTNTPRGSELL 116 Query: 118 EQLRKLEAQYLPVAVNIVKLATSQQNEAARTSLVKDCMPILNRVIAHVEQF----NKLLR 173 L++ A Y + +V L Q+ + A L +P+ ++ A V++ + L+ Sbjct: 117 AALKQARADYSKERIKVVDLIHQQKRDEATAHLFDKTIPVQDKYFAAVKEMVSFQSSLMD 176 Query: 174 KNSAANVDAAEAAYAMAKWTMMGASMAALVLGGLIAWLLTRSISRPLAQAVSVAQSVQSG 233 K++ A+ +A + M+ S A++L L A L+TRSI+RPL +AV VA +V +G Sbjct: 177 KSAERASAASSSAINI----MLVLSGVAVLLSALCAMLITRSITRPLNEAVQVATAVAAG 232 Query: 234 DLGSRIEVTSKDELGQLMAALKGMNDSLVAIVGEVRHGTDTIAVASDEIKRGNMDLSHRT 293 DL +IE TSKDE G L+ ALK MND+L IV EVR GTDTI AS EI GN+DLS RT Sbjct: 233 DLTVQIEATSKDETGALLGALKSMNDNLHRIVAEVRAGTDTINTASREIATGNLDLSSRT 292 Query: 294 EQQAGSLQETASAMEQLTATVKQTSDNAREAKRLADNASDVATKGGAAVNRVVETMHAIS 353 EQQAG+L+ETASAME+LT+TV+Q +DNAR+A LA+ AS VA +GG+ V VV+TM I+ Sbjct: 293 EQQAGALEETASAMEELTSTVRQNADNARQANTLAETASQVAIQGGSVVGEVVQTMGQIN 352 Query: 354 TSSHKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAVAAK 413 +S KIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSA AAK Sbjct: 353 DASRKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSAAAAK 412 Query: 414 EIEALINESVAKVATGEKQVSEAGSTMDEVVRSIQSVTHIVQDISAASTEQTAGLEQINT 473 EI++LI+ SV +V +G + V +AG+TM+EVV S++ VT +V +I+AAS EQ+ G+EQIN Sbjct: 413 EIKSLIDASVERVESGSRLVGQAGATMEEVVASVKRVTDVVAEITAASGEQSDGIEQINQ 472 Query: 474 AVARIDETTQQNAALVEQAAAAAASMQQQSVALTDVVSIFKLTAEQAG-PTAVRGPQQAA 532 A+ ++DE TQQNAALVEQAAAAA S+Q+QS L+D V IF+L + A P+ + P Q + Sbjct: 473 AIVQMDEVTQQNAALVEQAAAAAQSLQEQSGRLSDTVGIFRLGNDHAHQPSPLSAPVQRS 532 Query: 533 VQYSQDTAMPALS 545 VQ + A P L+ Sbjct: 533 VQVA--PARPVLA 543 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 394 bits (1013), Expect = e-111, Method: Compositional matrix adjust. Identities = 250/522 (47%), Positives = 341/522 (65%), Gaps = 18/522 (3%) Query: 1 MKNLKIGHRLGLGFAAVLVLLVAVATFGLQQVSRLNDRIVFLTSVDEGKLEALSKVQFAV 60 NLKI +L + FAAV+VL V + F ++++ +N + + ++A +Q ++ Sbjct: 4 FNNLKIAKKLIVSFAAVIVLTVILGLFAIRELKAVNQASTDMATNWLPTIKAAQSIQASL 63 Query: 61 GLRAIAARNLTLVNGAAEQ-------KGDLDLVGSAQKDIESGMAELTRLMQDPATTPQE 113 I+ + AE+ K LD++ + E+ ++E +E Sbjct: 64 PRMRISELQMVTAATPAEREDALKAIKSRLDILAKQRAAYEAQISE-----------AEE 112 Query: 114 RDMLEQLRKLEAQYLPVAVNIVKLATSQQNEAARTSLVKDCMPILNRVIAHVEQFNKLLR 173 + + E+ K A YL + + +++ + E AR D +I ++++ K+ Sbjct: 113 KVLYEKFSKSFATYLEIDKELARISAEGKEEEARALFKGDSNKTFRSMIENMDEIIKVND 172 Query: 174 KNSAANVDAAEAAYAMAKWTMMGASMAALVLGGLIAWLLTRSISRPLAQAVSVAQSVQSG 233 S + D AE Y A ++G A +V+ +A ++ R++SRPL +AV+VA+ V G Sbjct: 173 AGSKRSDDMAEQVYRSATLWIIGLLAAIVVIALGLAVMVARAVSRPLNEAVAVAERVAQG 232 Query: 234 DLGSRIEVTSKDELGQLMAALKGMNDSLVAIVGEVRHGTDTIAVASDEIKRGNMDLSHRT 293 DL I TS DE G LM +L+ MNDSL IVGEVR GTDTI AS EI GN+DLS RT Sbjct: 233 DLTMDIRATSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGNLDLSSRT 292 Query: 294 EQQAGSLQETASAMEQLTATVKQTSDNAREAKRLADNASDVATKGGAAVNRVVETMHAIS 353 E+QAGSL+ETASAME+LTATVKQ +DNAR+A +LA +ASD+AT+GGA V +VV+TM I+ Sbjct: 293 EEQAGSLEETASAMEELTATVKQNADNARQANQLAASASDIATQGGAVVGQVVQTMEGIT 352 Query: 354 TSSHKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAVAAK 413 SS++I DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA AAK Sbjct: 353 ESSNRIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAK 412 Query: 414 EIEALINESVAKVATGEKQVSEAGSTMDEVVRSIQSVTHIVQDISAASTEQTAGLEQINT 473 EI+ LI++SV KV G + V AG+TM EVV S++ VT +V +ISAAS EQ+ G+E+IN Sbjct: 413 EIKQLIDDSVEKVGAGSQLVERAGATMSEVVASVRRVTDVVAEISAASNEQSTGIEEINR 472 Query: 474 AVARIDETTQQNAALVEQAAAAAASMQQQSVALTDVVSIFKL 515 A+ ++DE TQQNAALVE+AAAAA S+Q Q+ L+ VVS+FKL Sbjct: 473 AITQMDEVTQQNAALVEEAAAAAQSLQDQAARLSQVVSVFKL 514 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.124 0.320 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,148,916 Number of extensions: 37040 Number of successful extensions: 528 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 265 Number of HSP's successfully gapped: 70 Length of query: 547 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 457 Effective length of database: 1,193,720 Effective search space: 545530040 Effective search space used: 545530040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)