BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0031.p (421 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter prote... 426 e-120 gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmemb... 95 1e-20 gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirill... 70 5e-13 gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysi... 62 6e-11 >gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter protein [Herbaspirillum seropedicae SmR1] Length = 292 Score = 426 bits (1094), Expect = e-120, Method: Compositional matrix adjust. Identities = 211/251 (84%), Positives = 219/251 (87%), Gaps = 1/251 (0%) Query: 1 LHDAHERNLIDADALSMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSH 60 LHDAHERNLIDADALSMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSH Sbjct: 40 LHDAHERNLIDADALSMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSH 99 Query: 61 SRFPAVDGERDKVIGILLAKDLLRYYAEESFDVRDMLRPAIFIPESKRLNVLLRDFRANR 120 SRFPAVDGERDKV+GILLAKDLLRYYAEESFDVRDMLRPAIFIPESKRLNVLLRDFRANR Sbjct: 100 SRFPAVDGERDKVVGILLAKDLLRYYAEESFDVRDMLRPAIFIPESKRLNVLLRDFRANR 159 Query: 121 NHMAVVVDEYGGVAGLITIEDVLEQIVGDIEDEYDFDEAEDNVLALKDGPHGARWRVKAL 180 NHMAVVVDEYGGVAGLITIEDVLEQIVGDIEDEYDFDEAEDNVLALKDGPHGARWRVKAL Sbjct: 160 NHMAVVVDEYGGVAGLITIEDVLEQIVGDIEDEYDFDEAEDNVLALKDGPHGARWRVKAL 219 Query: 181 TEIEQFNAEVGSRFSDEDADTIGGLVAAASPTKTPTPSADCAPMRARYTCCWCPSCRRHR 240 TEIEQFN EVGSRFSD+DADTIGGLV A + P + + + + H Sbjct: 220 TEIEQFNEEVGSRFSDDDADTIGGLV-ANHVGRVPRKGDEFDIDGLHFEVLRADARQVHV 278 Query: 241 WTTSELPDLPA 251 S+LP PA Sbjct: 279 LLVSQLPPAPA 289 >gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 434 Score = 94.7 bits (234), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 41/138 (29%), Positives = 85/138 (61%), Gaps = 4/138 (2%) Query: 16 SMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSHSRFPAVDGERDKVIG 75 S++ +F + ++V D+M PRSQ++ ++++ P++ + ++ H++ P +GE + ++G Sbjct: 194 SILLNLFDLEKISVEDVMTPRSQVEALNLAAPVDDILHQLATCYHNKLPVFEGEINHIVG 253 Query: 76 ILLAKDLLRYYA-EESF---DVRDMLRPAIFIPESKRLNVLLRDFRANRNHMAVVVDEYG 131 IL + + + EE D+R++L ++P + L+ F+ N + VVVDEYG Sbjct: 254 ILHVRKTVSLFRREEEISHDDIRELLSAPYYVPVETDVFTQLQYFQENHERLGVVVDEYG 313 Query: 132 GVAGLITIEDVLEQIVGD 149 V GL+T+ED++E+++G+ Sbjct: 314 EVQGLVTLEDIIEEMIGE 331 >gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 69.7 bits (169), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 55/193 (28%), Positives = 95/193 (49%), Gaps = 19/193 (9%) Query: 22 FQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSHSRFPAVDGERDKVIGILLAKD 81 ++L V DIM P S++ + K +E + + +SR+P D ER +V+G++ KD Sbjct: 214 LNFAELDVADIMRPASEIVALGDDKSLEENLDIIYRNRYSRYPYYDAERQQVLGLVHLKD 273 Query: 82 LLRYYAEES----FDVRDMLRPAIFIPESKRLNVLLRDFRANRNHMAVVVDEYGGVAGLI 137 + + A++ +++D LRP +I + LLR FR H AV+ + AG I Sbjct: 274 V--FLAQQDGRAIANLKDYLRPVQYISPALPALDLLRRFRTGSPHFAVIGKKGQPPAGFI 331 Query: 138 TIEDVLEQIVGDIEDEYDFDEAE----DNVLALKDGPHGARWRVKALTEIEQFNAEVGSR 193 T++++L +VG+I DE+ + E D+ L G + L + + E Sbjct: 332 TLDNMLSLLVGEIRDEFRHNTGEWTRQDDGTLLGKGS----LPIVTLENMLGIDIE---- 383 Query: 194 FSDEDADTIGGLV 206 D+ D++GGLV Sbjct: 384 -YDDSIDSVGGLV 395 >gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 62.4 bits (150), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 43/137 (31%), Positives = 72/137 (52%), Gaps = 3/137 (2%) Query: 17 MIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSHSRFPAVDGERDKVIGI 76 +IE VF++ V M PR + ++ T +++ HSRF DG D V+G Sbjct: 202 LIENVFELESRYVPSAMTPRDSIVYFLLTDSEATIRQKIIDHPHSRFLVCDGHIDAVLGY 261 Query: 77 LLAKDLL-RYYAEESFDVRD--MLRPAIFIPESKRLNVLLRDFRANRNHMAVVVDEYGGV 133 + +KD+L R E F + D ++ P + IP+S L +L +A +A+VV+EY V Sbjct: 262 IDSKDVLKRLLKGEQFSLADKGLVHPPLSIPDSLNLWEVLERMKAMDEDLAIVVNEYALV 321 Query: 134 AGLITIEDVLEQIVGDI 150 G+I+ DV ++G++ Sbjct: 322 VGIISTTDVTGVLMGNL 338 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.135 0.462 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,194,237 Number of extensions: 47230 Number of successful extensions: 181 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 178 Number of HSP's successfully gapped: 4 Length of query: 421 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 333 Effective length of database: 1,203,190 Effective search space: 400662270 Effective search space used: 400662270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 132 (55.5 bits)