BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0016.p (531 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane... 174 1e-44 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 173 3e-44 gi|300309883|ref|YP_003773975.1| GGDEF domain/EAL domain signal ... 171 2e-43 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 168 1e-42 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 163 3e-41 >gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 797 Score = 174 bits (442), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 94/227 (41%), Positives = 137/227 (60%), Gaps = 3/227 (1%) Query: 19 FWPALQPLVHLRSGRIAGFEMLARWTCPQRGVVSPIDFIDIAERGGVLDVLLLKLLDTVA 78 F LQP + L SG++ G E L RW P++G + P +FI AE G + +L L +++ VA Sbjct: 547 FMLYLQPKLELCSGQVVGAEALVRWIHPEKGFIGPDNFIPFAETTGFIRMLTLWMMEQVA 606 Query: 79 ---ATLRHWPPELRLAINLSPCQFLDGSLLDRLAAVMHQHGLPMSRLQLEITETVLVQDH 135 L+ +L+ ++NLS LD L AA++ +H L + L LEITE+ ++ D Sbjct: 607 RLSEQLQQQGVQLKFSVNLSTRDLLDQELPTAFAAILQRHRLQPAALCLEITESAIMDDP 666 Query: 136 ERTLQTILAARQAGASLSLDDFGTGYSSLTRLQSYPFDEIKIDASFVRTMDQNPDSFRIV 195 R T+ G +LS+DDFGTGYSSL L+ P DE+KID SFV M Q+ D +IV Sbjct: 667 ARAQNTLERLSNMGFALSIDDFGTGYSSLAYLKRLPVDELKIDKSFVMKMAQDQDDAKIV 726 Query: 196 LAVAGLGQSLKMHVVAEGIETEHHAHLLKRMGCEFGQGFHLGRPAPL 242 + LG +L + VVAEG+E+E LL++MGC+ GQG+ +G+P P+ Sbjct: 727 RSTVDLGHNLGLRVVAEGVESEEVWFLLRQMGCDQGQGYFMGKPMPV 773 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 173 bits (439), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 99/244 (40%), Positives = 146/244 (59%), Gaps = 5/244 (2%) Query: 4 LQPLVHLRSGRIAGXFWPALQPLVHLRSGRIAGFEMLARWTCPQRGVVSPIDFIDIAERG 63 L+ L++LR F QP + L+ GR+ G E L RW PQ+G+V P FI AE Sbjct: 440 LEMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWESPQQGMVPPDRFIPFAEEA 499 Query: 64 GVLDVLLLKLLDTVAATLRHWP----PELRLAINLSPCQFLDGSLLDRLAAVMHQHGLPM 119 G++ + +L T + W P + +A+NLSP Q L+ SL D +A V+ Q GL Sbjct: 500 GLIVPIGEWVLRTACRQNQAWQQAGLPPIPVAVNLSPRQ-LNQSLPDFVAGVLAQSGLAA 558 Query: 120 SRLQLEITETVLVQDHERTLQTILAARQAGASLSLDDFGTGYSSLTRLQSYPFDEIKIDA 179 S L+LEITE V+++D E+++ T+ A ++ G +S+DDFGTGYSSL+ L+ +P D +KID Sbjct: 559 SWLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSLSYLRRFPVDALKIDK 618 Query: 180 SFVRTMDQNPDSFRIVLAVAGLGQSLKMHVVAEGIETEHHAHLLKRMGCEFGQGFHLGRP 239 SFVR + ++ DS IV A+ L L + V+AEG+E E LK C+ QG++ G+P Sbjct: 619 SFVRDIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQHAFLKENACDEVQGYYFGKP 678 Query: 240 APLA 243 +A Sbjct: 679 MEVA 682 >gi|300309883|ref|YP_003773975.1| GGDEF domain/EAL domain signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 650 Score = 171 bits (432), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 95/239 (39%), Positives = 137/239 (57%), Gaps = 2/239 (0%) Query: 23 LQPLVHLRSGRIAGFEMLARWTCPQRGVVSPIDFIDIAERGGVLDVLLLKLLDTVAATLR 82 QP+V L G++ FE LARW +G VSP++FI AER G+++ L + LL A Sbjct: 414 FQPIVRLDDGQVIAFEALARWHSTVKGAVSPVEFIPAAERMGIINQLSVILLRKALAAAA 473 Query: 83 HWPPELRLAINLSPCQFLDGSLLDRLAAVMHQHGLPMSRLQLEITETVLVQDHERTLQTI 142 WP ++RL+ NLS + + ++ + G SRL LEITET L+QD E+T Q I Sbjct: 474 SWPAQIRLSFNLSANDINSSEGMAEIIKLIRESGFCPSRLDLEITETALLQDLEKTRQLI 533 Query: 143 LAARQAGASLSLDDFGTGYSSLTRLQSYPFDEIKIDASFVRTMDQNPDSFRIVLAVAGLG 202 + RQ G ++LDDFGTGY+SL+ L + P IKID SFV + Q+ S +IV ++ L Sbjct: 534 HSLRQLGCGITLDDFGTGYASLSHLHALPLTRIKIDKSFVADIGQDAVSLKIVKSLLALT 593 Query: 203 QSLKMHVVAEGIETEHHAHLLKRMGCEFGQGFHLGRPAPLADAARLIGERGVWSRHSAG 261 + + V EG+ET H +L+ +G +GQG+ RP D A + +R +R AG Sbjct: 594 LDMDLACVVEGLETPQHLAVLRELGAMYGQGYLYARPMAADDVAPWLAQRA--ARVQAG 650 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 168 bits (425), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 89/236 (37%), Positives = 135/236 (57%), Gaps = 4/236 (1%) Query: 19 FWPALQPLVHLRSGRIAGFEMLARWTCPQRGVVSPIDFIDIAERGGVLDVLLLKLLDTVA 78 F QP + L GRI G E L RW P RG++ P +FI +AE ++D + L +LD Sbjct: 858 FVVHYQPKIELAEGRIVGAEALVRWQHPLRGLLPPGEFITLAEETRLIDQIGLLVLDVAC 917 Query: 79 ---ATLRHWPPEL-RLAINLSPCQFLDGSLLDRLAAVMHQHGLPMSRLQLEITETVLVQD 134 +R R++INL+ QF D LLD + V+ +HG P S L+ EITE++++ + Sbjct: 918 RDIGLMRQQQMTCGRISINLTGSQFGDERLLDDIKEVVDRHGTPPSCLEFEITESMVMHN 977 Query: 135 HERTLQTILAARQAGASLSLDDFGTGYSSLTRLQSYPFDEIKIDASFVRTMDQNPDSFRI 194 ER + + R G ++S+DDFGTGYSSL L+ +P + +KID SF+ + NP+S I Sbjct: 978 RERAIAIMDGIRAEGFTISIDDFGTGYSSLAYLKRFPVNTLKIDKSFINDIPDNPNSSAI 1037 Query: 195 VLAVAGLGQSLKMHVVAEGIETEHHAHLLKRMGCEFGQGFHLGRPAPLADAARLIG 250 V A+ + +L + VV EG+ETE L+ G + QG++ +P P A +L+ Sbjct: 1038 VQAIIAMSHALNLKVVTEGVETEKQLQALREFGSDEYQGYYFSKPVPYAQFVQLLA 1093 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 163 bits (413), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 90/236 (38%), Positives = 132/236 (55%), Gaps = 4/236 (1%) Query: 10 LRSGRIAGXFWPALQPLVHLRSGRIAGFEMLARWTCPQRGVVSPIDFIDIAERGGVLDVL 69 LR+ G F QP V L SGR+ G E L RW PQ G+VSP FI +AE G++ + Sbjct: 698 LRNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQLGMVSPARFIGLAEETGLILPI 757 Query: 70 LLKLLDTVAATLRHWP----PELRLAINLSPCQFLDGSLLDRLAAVMHQHGLPMSRLQLE 125 +L R W ++ +++NLS QFL L+ +A V+ GLP L+LE Sbjct: 758 GAWVLRAACTQCRQWQLGGYADISVSVNLSVGQFLQKDLVSSIADVLEDTGLPPESLELE 817 Query: 126 ITETVLVQDHERTLQTILAARQAGASLSLDDFGTGYSSLTRLQSYPFDEIKIDASFVRTM 185 +TE++++ D E + + ++ G LS+DDFGTGYSSL L+ +P D +KID SFVR + Sbjct: 818 LTESLMMTDVEHAIGILAGLKKLGVRLSIDDFGTGYSSLAYLKRFPIDVLKIDRSFVRDI 877 Query: 186 DQNPDSFRIVLAVAGLGQSLKMHVVAEGIETEHHAHLLKRMGCEFGQGFHLGRPAP 241 + D I ++ L + LK+ V+AEG+ET+ L+ GC+ QG+ P P Sbjct: 878 TIDADDAAITASIISLARHLKLRVIAEGVETDEQLDYLRHYGCDEIQGYLFSPPVP 933 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.138 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,746,409 Number of extensions: 76864 Number of successful extensions: 292 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 270 Number of HSP's successfully gapped: 18 Length of query: 531 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 441 Effective length of database: 1,193,720 Effective search space: 526430520 Effective search space used: 526430520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)