BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0030.0010.p
         (760 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310040|ref|YP_003774132.1| organic solvent tolerance trans...  1331   0.0  

>gi|300310040|ref|YP_003774132.1| organic solvent tolerance
           transmembrane protein [Herbaspirillum seropedicae SmR1]
          Length = 766

 Score = 1331 bits (3444), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 655/768 (85%), Positives = 712/768 (92%), Gaps = 10/768 (1%)

Query: 1   MIRFSKSFTERTVNWRLARLFSSAMVVASASALLPLSVHAQMSMPTAAPATPPAQDQDAP 60
           MIRFSKS TERTVNWRLARLFSSAM VASASALLPLSV+AQM++P  AP   PAQDQDAP
Sbjct: 1   MIRFSKSLTERTVNWRLARLFSSAMAVASASALLPLSVYAQMTLP--APVAAPAQDQDAP 58

Query: 61  VNVSAEQMTGRPDRQVNLDRDVELVKAATTIKSDKAEYRIIQDEVEATGHVWMQRLQDQY 120
           VNVSAEQMTGRPDRQVNLDRDVE+VK  TTIKSDKAEYRIIQDEVEATGHVWM+RLQDQY
Sbjct: 59  VNVSAEQMTGRPDRQVNLDRDVEVVKGPTTIKSDKAEYRIIQDEVEATGHVWMRRLQDQY 118

Query: 121 TGDYAEMNMGTGAGYVTNPTYLIGKNGGRGKAERIDFLSDDQAKVTQGTYSTCEAPDPDW 180
           TGD+AEMNMGTGAGYV++PTYLIGKNGGRGKAERIDF+SDDQA VTQGTYSTCEAPDPDW
Sbjct: 119 TGDHAEMNMGTGAGYVSSPTYLIGKNGGRGKAERIDFISDDQALVTQGTYSTCEAPDPDW 178

Query: 181 YLRANTMDLDSGRDIGTMHGGLLYFKDVPILGSPWMTFPLSGERKSGVLPPTIGMTSTGG 240
           YLRANTMDLDSGRD+GT+ GGLLYFKDVPIL +PWM+F LSGERKSGVLPPTIG TSTGG
Sbjct: 179 YLRANTMDLDSGRDVGTVRGGLLYFKDVPILAAPWMSFALSGERKSGVLPPTIGATSTGG 238

Query: 241 AEISVPYYFNLAPNYDLTLVPKYIARRGLQMGAETRYIGDTYSGITNVEGIQDQLTGTGR 300
            E++VPYYFNLAPNYDLTLVPKYIARRGLQMGAETRY+G++Y+G+ N EGIQD++TGTGR
Sbjct: 239 FELAVPYYFNLAPNYDLTLVPKYIARRGLQMGAETRYMGESYNGVFNAEGIQDRVTGTGR 298

Query: 301 YSLSSIHNQTLAPGLQLAWNLNKASDNDYPSDFAHSITGSTQRLLPRNVSLSYASTYWSV 360
           YSLSS H+Q LAPGLQL WNLNKASDNDY SDF+HSITGST RLLPR  SL+Y+STYW++
Sbjct: 299 YSLSSRHSQILAPGLQLDWNLNKASDNDYASDFSHSITGSTTRLLPRTASLTYSSTYWTL 358

Query: 361 TGLVSKYQLLQDVNAPIGKPYDRVPQITFTGNRYDIGGFDFSTTADFTRFSSGDLIGGDR 420
            G VSKYQLLQDVNAPI KPYDRVPQITF G RYDIGGFDF+T ADFTRFSSG L+GGDR
Sbjct: 359 VGQVSKYQLLQDVNAPIVKPYDRVPQITFNGTRYDIGGFDFNTVADFTRFSSGALVGGDR 418

Query: 421 AYVTQSVSYPIIRPGYFITPKVILDATSYNLNNVSAGQPTNLTRTVPTVSLDSGLIFERD 480
           AY+TQSVSYPI+RPGYFITPKV LDATSY LNN++ GQ TNLTRTVPT+SLDSGLIFER+
Sbjct: 419 AYLTQSVSYPIVRPGYFITPKVTLDATSYKLNNLAPGQQTNLTRTVPTLSLDSGLIFERE 478

Query: 481 ANLFGRSVTQTLEPRLFYVRTPYRDQSQYPVFDSGLADFNFAQIFTENRFVGHDRISDAN 540
           ++L GRS+TQTLEPRLFYVRTPYRDQ++ P+FDSGLADFNFAQIF+ENRFVGHDRISDAN
Sbjct: 479 SDLLGRSMTQTLEPRLFYVRTPYRDQTKLPIFDSGLADFNFAQIFSENRFVGHDRISDAN 538

Query: 541 QLTAAVTSRLIEENGLERLRAAIGQRYYFADPKV--------AVSGTTTTTLGSKSDLLM 592
           QLTAAVTSRLIEENGLERLRA +GQRYYFADPKV        A + T++ +LGSKSDLL+
Sbjct: 539 QLTAAVTSRLIEENGLERLRATVGQRYYFADPKVDLTSTSTTATTTTSSGSLGSKSDLLL 598

Query: 593 ALGGQITREFSTDNTVQYSETLRQIVRSTFGIRWQPAPKRVLNLQYRLDRTYINPYTGVL 652
           ALGGQITREFSTDNTVQYSETLRQ+VRS FGIRWQPAPKRVLNLQYRLDRTY+NPYTGVL
Sbjct: 599 ALGGQITREFSTDNTVQYSETLRQMVRSNFGIRWQPAPKRVLNLQYRLDRTYVNPYTGVL 658

Query: 653 DPLKQVDVSGQWPITQRIYAVGRMNYSIPDRTVAEGLAGFEYKADCWVFRVVAQRIPTSS 712
           DPLKQVDVSGQWP++QRIYAVGR+NYSIPDRTVAEGL G EYKADCWVFRVVAQRIPTSS
Sbjct: 659 DPLKQVDVSGQWPLSQRIYAVGRINYSIPDRTVAEGLLGLEYKADCWVFRVVAQRIPTSS 718

Query: 713 TKASTGFFIQLELNGLSKIGSNPLDAIKSSVPGYQNVNLPDTMPGNNF 760
           TKASTGFF QLELNG S+IGSNP+DAIKSSVPGYQ VNLPD+ PGNNF
Sbjct: 719 TKASTGFFFQLELNGFSQIGSNPMDAIKSSVPGYQKVNLPDSTPGNNF 766


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.317    0.134    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 2,312,246
Number of extensions: 101542
Number of successful extensions: 190
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 188
Number of HSP's successfully gapped: 1
Length of query: 760
Length of database: 1,619,870
Length adjustment: 93
Effective length of query: 667
Effective length of database: 1,179,515
Effective search space: 786736505
Effective search space used: 786736505
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 134 (56.2 bits)