BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0006.p (266 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310036|ref|YP_003774128.1| 2-octaprenyl-6-methoxyphenol hy... 438 e-124 gi|300313476|ref|YP_003777568.1| 2-polyprenyl-6-methoxyphenol hy... 66 3e-12 >gi|300310036|ref|YP_003774128.1| 2-octaprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 438 bits (1127), Expect = e-124, Method: Compositional matrix adjust. Identities = 224/256 (87%), Positives = 236/256 (92%), Gaps = 1/256 (0%) Query: 5 TARWPRLSREXPPPAIAPLGLTQMRPAEAQEITEEDDHVAVTLKDGCVLRAQLVIQAEGG 64 AR+ ++R AIAPLGL+ MRPAEAQ+I EED HVAVTLKDG +LRA LVIQAEGG Sbjct: 107 VARYGEVNRALAS-AIAPLGLSLMRPAEAQQIEEEDQHVAVTLKDGRILRAHLVIQAEGG 165 Query: 65 VFGEQQQKSLQRDYQQLGIIAHVQADGVIAHRAFERFTDEGPLALLPQDDGYALVWCVRP 124 VFGEQQ K+LQRDYQQLGIIAHVQADGVIAHRAFERFTDEGPLALLPQDDGYALVWCVRP Sbjct: 166 VFGEQQHKTLQRDYQQLGIIAHVQADGVIAHRAFERFTDEGPLALLPQDDGYALVWCVRP 225 Query: 125 ATGEALLALDDAAFLASLQRTFGERVGRFTRISARHAFPLGLNARPGQSRRVVAIGNAAQ 184 ATGE L+AL+DA FLA+LQRTFGERVGRFTRIS RHAFPLGLNARPGQSRRVVAIGNAAQ Sbjct: 226 ATGEQLMALEDAGFLAALQRTFGERVGRFTRISPRHAFPLGLNARPGQSRRVVAIGNAAQ 285 Query: 185 TLHPVAGQGLNLGLRDARVLADVLAREVSPEALQAFERQRGQDRGLTIRLTDLMARIFAG 244 TLHPVAGQGLNLGLRDARVLADVLARE+SP+ALQAFERQRGQDR LTIRLTDLMARIFAG Sbjct: 286 TLHPVAGQGLNLGLRDARVLADVLAREISPQALQAFERQRGQDRSLTIRLTDLMARIFAG 345 Query: 245 SPQGSPLQAALGLXGG 260 SPQGSPLQAALGL G Sbjct: 346 SPQGSPLQAALGLSLG 361 >gi|300313476|ref|YP_003777568.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 410 Score = 66.2 bits (160), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 71/230 (30%), Positives = 111/230 (48%), Gaps = 20/230 (8%) Query: 45 VTLKDGCVLRAQLVIQAEGGVFGEQQQKSLQRDYQQLG---IIAHVQADGVIAHR--AFE 99 +TL+ G L A LVI A+G + Q + DY+ G I+ + + + HR A++ Sbjct: 164 LTLESGQSLSASLVIGADGANSWVRGQCDIGLDYRAYGQNAIVTNFSCE--LPHRGVAYQ 221 Query: 100 RFT-DEGPLALLP-QDDGYALVWCVRPATGEALLALDDAAFLASLQRTFGERVGRFTRI- 156 F+ EG +ALLP + +LVW A + LL L +A L + +G T + Sbjct: 222 WFSPTEGVIALLPLPGNRVSLVWSAPQALADQLLKLPLSALAERLAQWAAPVLGALTPLQ 281 Query: 157 -SARHAFPLGLNARPGQ--SRRVVAIGNAAQTLHPVAGQGLNLGLRDARVLADVLAR--- 210 AFPL L +P + RV IG+AA +HP+AG G+NLG D L D+L + Sbjct: 282 PEVAKAFPLRL-IKPHAMIAPRVALIGDAAHVVHPMAGHGMNLGFGDVAALIDILLKREP 340 Query: 211 --EVSPEALQAFERQRGQDRGLTIRL-TDLMARIFAGSPQGSPLQAALGL 257 + E L + ++ L +++ TD + RIF+ + L LG+ Sbjct: 341 QYDCGDERLLRRYARARKEEVLLMQIATDGLHRIFSTEAEPVRLVRNLGM 390 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 855,410 Number of extensions: 35424 Number of successful extensions: 120 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 121 Number of HSP's successfully gapped: 2 Length of query: 266 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 182 Effective length of database: 1,222,130 Effective search space: 222427660 Effective search space used: 222427660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)