BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0029.0089.p
         (380 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311906|ref|YP_003775998.1| L-lactate dehydrogenase [Herbas...   756   0.0  
gi|300309586|ref|YP_003773678.1| FMN-dependent L-lactate dehydro...   241   5e-65

>gi|300311906|ref|YP_003775998.1| L-lactate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 380

 Score =  756 bits (1953), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 369/379 (97%), Positives = 379/379 (100%)

Query: 1   MPVMTCVEDFRLLAKKRVPKAFYDYADSGSYTESTYRANTRDLAALKLRQRVAINVDERS 60
           MPVMTCVEDFRLLAKKRVPKAFYDYADSGSYTESTYRAN++DLAALKLRQRVAINVDERS
Sbjct: 1   MPVMTCVEDFRLLAKKRVPKAFYDYADSGSYTESTYRANSKDLAALKLRQRVAINVDERS 60

Query: 61  TRSTMIGHDVTMPVAIAPTGLTGMQWANGEMLGAIAAEKFGIPFTLSTMSICSIEDVASV 120
           TR+TMIGHDVTMPVAIAPTGLTGMQWANGEMLGAIAAEKFGIPFTLSTMSICSIEDVASV
Sbjct: 61  TRTTMIGHDVTMPVAIAPTGLTGMQWANGEMLGAIAAEKFGIPFTLSTMSICSIEDVASV 120

Query: 121 TTKPFWFQLYVMRDRGFVQSLIDRAKAAKCSALVLTLDLQILGQRHKDLKNGMSVPPKLT 180
           TTKPFWFQLYVMRDRGFV+SLI+RAKAAKCSALVLTLDLQILGQRHKDLKNGMSVPPKLT
Sbjct: 121 TTKPFWFQLYVMRDRGFVKSLIERAKAAKCSALVLTLDLQILGQRHKDLKNGMSVPPKLT 180

Query: 181 LDTLLDLASKPGWALRALGGRKTFGNLAGHIKGGEGAGGVQTLSKWTASQFDPTLNWEDV 240
           L+TLLDLASKPGWALRALGGRKTFGNLAGHIKGGEGAGGVQTLSKWTASQFDPTLNW+DV
Sbjct: 181 LETLLDLASKPGWALRALGGRKTFGNLAGHIKGGEGAGGVQTLSKWTASQFDPTLNWDDV 240

Query: 241 AWIKQQWGGKLILKGILDVEDAKLAVQSGADAIVVSNHGGRQLDGAMSSIEALPAIAEAV 300
           AWIKQQWGGKLILKGILDVEDAKLAVQSGADAIVVSNHGGRQLDGAMSSIEALPAIA+AV
Sbjct: 241 AWIKQQWGGKLILKGILDVEDAKLAVQSGADAIVVSNHGGRQLDGAMSSIEALPAIAQAV 300

Query: 301 GDQIEVWFDGGIRSGQDVLKAVALGARGTMIGRAFLYSLGAMGGDGVSQMLEIMRKELDV 360
           GDQIEVWFDGGIRSGQDVLKAVALGARGTMIGRAFLYSLGAMGG+GVSQMLEIMRKELDV
Sbjct: 301 GDQIEVWFDGGIRSGQDVLKAVALGARGTMIGRAFLYSLGAMGGEGVSQMLEIMRKELDV 360

Query: 361 SMALTGTKDIKDVGPQILV 379
           SMALTGTKDIKDVGPQIL+
Sbjct: 361 SMALTGTKDIKDVGPQILI 379


>gi|300309586|ref|YP_003773678.1| FMN-dependent L-lactate
           dehydrogenase [Herbaspirillum seropedicae SmR1]
          Length = 413

 Score =  241 bits (616), Expect = 5e-65,   Method: Compositional matrix adjust.
 Identities = 146/383 (38%), Positives = 208/383 (54%), Gaps = 18/383 (4%)

Query: 7   VEDFRLLAKKRVPKAFYDYADSGSYTESTYRANTRDLAALKLRQRVAINVDERSTRSTMI 66
           +E+ R +A+KRVP   ++Y + G+  E + R N      +    R  ++V  R     + 
Sbjct: 37  IEELRAMARKRVPNFCFEYVEGGAEEEISLRHNREVFTRIGFLPRTLVDVSVRRQGRRLF 96

Query: 67  GHDVTMPVAIAPTGLTGMQWANGEMLGAIAAEKFGIPFTLSTMSICSIEDVASVTTKPFW 126
           G D+  P  I PTG +G+    G++  A AA   G+PF L+ +S  S+E+V   +    W
Sbjct: 97  GQDIASPFLIGPTGFSGLLAREGDVAMASAAASAGVPFVLTNVSTTSLEEVVRRSGAQVW 156

Query: 127 FQLYVMRDRGFVQSLIDRAKAAKCSALVLTLDLQILGQRHKDLKNGMSVPPKLTLDTLLD 186
            Q+Y+ RDR FV S+  RA+AA    LVLT D  + G+R  D +N  S P +L     LD
Sbjct: 157 QQVYLYRDRAFVASVAQRAQAAGIGVLVLTTDSAVYGKREWDARN-FSSPRRLDWRNKLD 215

Query: 187 LASKPGWALRAL--GGRKTFGNLAG-------HIKGGEGAGGVQTLSKWTASQFDPTLNW 237
           +   P W +  L   G   F NL          ++G   A   Q+LS          L+W
Sbjct: 216 VLRHPRWLIDILYPHGFPRFANLGDLLPPDQTSVRGAAAAILGQSLSA--------ALDW 267

Query: 238 EDVAWIKQQWGGKLILKGILDVEDAKLAVQSGADAIVVSNHGGRQLDGAMSSIEALPAIA 297
            DV W++  W GKL+LKG++ VEDA+ AV  G D IV+SNHGGRQLDGA+S+++ LP + 
Sbjct: 268 ADVQWLRGIWPGKLVLKGVMQVEDAQRAVALGVDGIVLSNHGGRQLDGALSTMDVLPEVV 327

Query: 298 EAVGDQIEVWFDGGIRSGQDVLKAVALGARGTMIGRAFLYSLGAMGGDGVSQMLEIMRKE 357
            AV  Q+ V  DGG R G D++KA+ALGA   ++GRA  Y L A G  G ++ LEI+R E
Sbjct: 328 AAVKGQLTVMLDGGFRRGADIVKAIALGADAVLLGRATTYGLAAGGQAGATRALEILRSE 387

Query: 358 LDVSMALTGTKDIKDVGPQILVR 380
           +D  +AL    DI  +    L R
Sbjct: 388 VDRVLALLACPDIDQLDASYLRR 410


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.135    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,129,643
Number of extensions: 45100
Number of successful extensions: 183
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 182
Number of HSP's successfully gapped: 2
Length of query: 380
Length of database: 1,619,870
Length adjustment: 87
Effective length of query: 293
Effective length of database: 1,207,925
Effective search space: 353922025
Effective search space used: 353922025
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 131 (55.1 bits)