BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0055.p (471 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum... 216 3e-57 gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) ... 70 5e-13 gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum s... 65 9e-12 gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirill... 63 5e-11 >gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 216 bits (550), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 152/463 (32%), Positives = 232/463 (50%), Gaps = 23/463 (4%) Query: 7 ILAIDAGSSSTRAAIVTADGLVSCLHRRPLRTHSARSGVVEQDAALIWQQTLTACRASLQ 66 +LA+D G+SS+R+ I G + ++ + G VE D +W L + L Sbjct: 4 LLALDQGTSSSRSIIFDQSGAIVATAQQEFPQIFPQPGWVEHDPMDLWHSQLQTAQKVLA 63 Query: 67 QARQKGLRVRAIGLCNQRASAVVWDTVSGEPLAPALSWQDSRHAAELAALAPHWDQRLRP 126 Q+ K + A+G+ NQR + VVW+ +G+P+ A+ WQD R AAL R Sbjct: 64 QSGIKASELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAAL--------RE 115 Query: 127 R-LGRPVGSRS-----PYLWAAHL---LRHQPRVARAWAQGRLGFGGIDTWLLWQLSGRR 177 R LG + ++ PY L L H + +G L FG +D+WL+W L+G R Sbjct: 116 RGLGEAIQKKTGLVLDPYFSGTKLRWILDHVDGLRARAERGELLFGTVDSWLVWNLTGGR 175 Query: 178 LLATSATNATACNAYVLDAHAYALDWLEALGFPATLLPALCQDADDYGRSDAAVLGAALP 237 L + +NA+ + + A+ + L+ LG PA +LP + A YG DA LGAA+ Sbjct: 176 LHVSDVSNASRTMLWNIHTGAWDEELLQWLGIPAAMLPQVQPSAHLYGEIDAQHLGAAVK 235 Query: 238 IRACAGDQLAAAIGQGCLEPGQALCQHGTGSFIDVLVGPRAALAPADSTAEASVVVTARR 297 I AGDQ +A GQ C+ PG A +GTG F+ + G + A + + A+ V ++ Sbjct: 236 IGGIAGDQQSALFGQACVRPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVDEQK 295 Query: 298 QNGVSHFALESYTATTGAALTWACQRLRWFPDARQLLALAASVSSAGGVSFLPALAGMQS 357 Q +ALE GA + W L+ + + LA SV +GGV F+P+ G+ + Sbjct: 296 Q-----YALEGSVFIGGAVVQWLRDGLKAIKTSTDVEGLACSVEDSGGVVFVPSFTGLGA 350 Query: 358 PQRQPAARAALQGLSLATGRAEVAHALVEGIAQSVTACVSASADTAGLPVSRIMSGGTLA 417 P P+A+ A+ GLS + A +A A +E IA TA ++A A P++ + G A Sbjct: 351 PYWNPSAKGAIVGLSRGSTVAHIARAALESIAFQSTALLNAMVRDAVSPITELRVDGGAA 410 Query: 418 SSDLLLQLQADLSGVTTCRTHQSAHASLRGIAFLAGSXKGGPR 460 +++LLLQ QADL G+ R + +L G A+LAG G R Sbjct: 411 ANNLLLQFQADLLGIPVIRPQVTETTAL-GAAYLAGIATGVYR 452 >gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) kinase [Herbaspirillum seropedicae SmR1] Length = 490 Score = 69.7 bits (169), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 116/441 (26%), Positives = 174/441 (39%), Gaps = 50/441 (11%) Query: 8 LAIDAGSSSTRAAIVTADGLVSCLHRRPLRTHSARSGVVEQDAALIWQQTL-TACRASLQ 66 L ID G+S +A ++ A G + L L EQ A WQ T T + Q Sbjct: 3 LGIDLGTSEVKAVVIDAQGSLVALAGSTLNVARPHPRWSEQAPADWWQATCDTVAKLRTQ 62 Query: 67 QARQKGLRVRAIGLCNQRASAVVWDTVSGEPLAPALSWQDSRHA---AELAALAPHWDQR 123 ++ +RAIGL Q AV+ D E L PA+ W DSR A AEL + AP Sbjct: 63 LGSERFGSIRAIGLSGQMHGAVLLDE-RDEVLRPAILWSDSRSAPECAELESRAPRL-HG 120 Query: 124 LRPRLGRPVGSRSPYLWAAHLLRHQPRVARAWAQGRLGFGGIDTWLLWQLSGRRLLATSA 183 + L P + LW A RH+P++ A L WL +++GR++ + Sbjct: 121 IAGNLAMPGFTAPKLLWVA---RHEPQLFARIATVLLP----KDWLRLKMTGRKV--SDP 171 Query: 184 TNATACNAYVLDAHAYALDWLEALGFPATLLPALCQDADDYGRSDAAVLGAALPIRACAG 243 ++A ++ ++ + L A G +PAL +AV G+ LP A A Sbjct: 172 SDAAGTLWLDVEGRNWSDELLAASGMRRDQMPALV--------DGSAVSGSLLPEVAQAW 223 Query: 244 DQLAAAI-------------GQGCLEPGQALCQHGTGSFIDVLVGPRAALAPADSTAEAS 290 + I G G ++PG GT + V V R P + Sbjct: 224 GLRSDVIVAGGAGDGAASAVGIGAVKPGDGFLSLGTSGVLFV-VNDRFRPNPGRAIHAFC 282 Query: 291 VVVTARRQNGVSHFALESYTATTGAALTWACQRLRWFPDARQLLALAASVSSAGGVS--- 347 + R S + + L W C+ D + LLA + + Sbjct: 283 HTLPQRWHQ-------MSVMLSAASCLRWFCRLCS--VDEKSLLAEIEQLDEQACNNAPL 333 Query: 348 FLPALAGMQSPQRQPAARAALQGLSLATGRAEVAHALVEGIAQSVTACVSASADTAGLPV 407 FLP L+G ++P P A GL+ RA + +A++EG+A + + A AG V Sbjct: 334 FLPYLSGERTPHNDPYATGVFHGLTPEHQRAALGYAVLEGVAFGMVDGLDA-LRAAGTDV 392 Query: 408 SRIMSGGTLASSDLLLQLQAD 428 + + G A S QL AD Sbjct: 393 AELSLVGGGARSAYWAQLLAD 413 >gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 65.5 bits (158), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 109/464 (23%), Positives = 175/464 (37%), Gaps = 82/464 (17%) Query: 7 ILAIDAGSSSTRAAIVTADGLVSCLHRRPLRTHSARSGVVEQDAALIWQQTLTACRASLQ 66 +L +D G++ST++ + T DG V H G+ EQD I L + +++ Sbjct: 10 MLGVDIGTTSTKSVVFTLDGKVVAQHAEEYPVLCTEPGMAEQDPEQIVAAALASIAGAVK 69 Query: 67 QARQKGLRVRAIGLCNQRASAVVWDTVSGEPLAPALSWQDSRHAAELAALAPHWDQRLRP 126 A+ + + S + D L+ +++W D R A W +R++ Sbjct: 70 AAKAAPQEIALLSFSAAMHSVIALDA-ENRLLSNSITWGDIR--------ASVWAERIKH 120 Query: 127 ---------RLGRPVGSRSP---YLWAAHLLRHQPRVARAWAQGRLGFGGIDTWLLWQLS 174 R G PV SP +W H +P V A+ F GI +LL+QL Sbjct: 121 EHDGNAIYRRTGTPVHPMSPLCKLMWMRH---EKPDVFHRAAR----FVGIKEYLLYQLF 173 Query: 175 GRRLLATSATNATAC---NAYVLDAHAYALDWLEALGFPATL-----LPALCQDADDYGR 226 G+ ++ S +AT D A AL + P + LPAL + Sbjct: 174 GQWVVDHSIASATGMFNLRELAWDQGALALLGVRPDQLPTPVPTTHRLPALSSEM----- 228 Query: 227 SDAAVLGAAL--PIRACAGDQLAAAIGQGCLEPGQALCQHGT-GSFIDVLVGPRAALAPA 283 A LG ++ P A D + + +G +E G GT G+ V+ PR Sbjct: 229 --AQRLGLSVDTPFIIGANDGVLSNLGVNAIEIGHVAVTIGTSGAMRTVIDEPR------ 280 Query: 284 DSTAEASVVVTARRQNGVSHFALESYTATTGAALTWACQRLRWFPDA------------- 330 Q + +AL G + RW D Sbjct: 281 -----------TDPQGRLFCYALTEKHWVVGGPVNNGGNIFRWVRDELATAEAAAAREEG 329 Query: 331 ----RQLLALAASV-SSAGGVSFLPALAGMQSPQRQPAARAALQGLSLATGRAEVAHALV 385 L +A V A G+ F P +AG ++P R + GL+L G+ + A + Sbjct: 330 VDPYEALTRIAEKVRPGAEGLLFHPFMAGERAPLWNADLRGSFFGLALHHGKHHMIRAAL 389 Query: 386 EGIAQSVTACVSASADTAGLPVSRIMSGGTLASSDLLLQLQADL 429 EG+ ++ + + A + G P R+M+ G A S L Q+ AD+ Sbjct: 390 EGVIFNLYSILPALEELVG-PTKRMMATGGFARSALWRQMMADI 432 >gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 63.2 bits (152), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 114/415 (27%), Positives = 159/415 (38%), Gaps = 76/415 (18%) Query: 8 LAIDAGSSSTRAAIVTADGLVSCLHRRPLRTHSARSGVVEQDAALIWQQTLTACRASLQQ 67 L ID G+S + + D V LR + EQ W TL A A Q Sbjct: 4 LGIDLGTSEVKLVLTDEDSNVIASTSARLRVDNPHPLWSEQAPQSWWNATLDAIAALRTQ 63 Query: 68 ARQKGLRVRAIGLCNQRASAVVWDTVSGEPLAPALSWQDSRHAAELAALAPHWDQRLRPR 127 A Q +R IGL Q A + D +G L PA+ W D R AE L + L P Sbjct: 64 APQAFQALRGIGLSGQMHGATLLDR-NGNVLRPAILWNDMRAHAECVEL-----EALVPD 117 Query: 128 LGRPVGSRS-PYLWAAHLL---RHQPRVARAWAQGRLGFGGIDTWLL------WQLSGRR 177 G+R+ P A LL +++P V RA ID LL W+L+G Sbjct: 118 AVAITGNRAMPGFTAPKLLWLSKYEPAVYRA----------IDKVLLPKDFLGWKLTGEF 167 Query: 178 LLATSATNATACNAYVLDAHAYALDWLE----ALGFPATLLPALCQDA------DDYGRS 227 + S A LD DW E A G + +P L + + D R Sbjct: 168 VSEMS----DAAGTLWLDVARR--DWSERMLGATGLDRSHMPRLVEGSAVAGQLRDELRR 221 Query: 228 DAAVLGAALPIRACAGDQLAAAIGQGCLEPGQALCQHGTGSFIDVLVGPRAALAPADSTA 287 + + G + + AGD A+A+G G + G A G+ VL A AP Sbjct: 222 EWGISGPVV-VAGGAGDNAASAVGIGVIRAGDAFLSLGSSG---VLFAATAHHAPNAQQG 277 Query: 288 -------------EASVVVTARRQNGVSHFALESYTATTGAALTWACQRLRWFPDARQLL 334 + SV+++A S L TA A L +RL A+ L Sbjct: 278 VHAFCHCLPGQWHQMSVILSA----AASLHWLSGVTARPVAELVSGAERLSAGQQAQAPL 333 Query: 335 ALAASVSSAGGVSFLPALAGMQSPQRQPAARAALQGLSLATGRAEVAHALVEGIA 389 FLP L G ++P AA+ L G++ A A +A+A++EG+A Sbjct: 334 -------------FLPYLNGERTPHNDAAAKGVLFGMTPAHESAHLAYAVMEGVA 375 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.130 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,389,277 Number of extensions: 57944 Number of successful extensions: 222 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 219 Number of HSP's successfully gapped: 4 Length of query: 471 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 382 Effective length of database: 1,198,455 Effective search space: 457809810 Effective search space used: 457809810 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)