BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0051.p (290 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311938|ref|YP_003776030.1| hypothetical protein Hsero_2626... 515 e-147 gi|300312232|ref|YP_003776324.1| signal transduction protein [He... 240 1e-64 gi|300311595|ref|YP_003775687.1| two component response regulato... 67 2e-12 gi|300310564|ref|YP_003774656.1| regulator of the two-component ... 64 1e-11 >gi|300311938|ref|YP_003776030.1| hypothetical protein Hsero_2626 [Herbaspirillum seropedicae SmR1] Length = 290 Score = 515 bits (1327), Expect = e-147, Method: Compositional matrix adjust. Identities = 250/290 (86%), Positives = 269/290 (92%) Query: 1 MELKSLVDQPGKLPTIPKVVQQLMTSFNSEEVSAQDIAHQIAADPTLTAKLLRLANSAFF 60 MELKSLVDQPGKLPT+PKVVQQLM SFN+E++SA +IA QIA DP L+AK+LRLANSAFF Sbjct: 1 MELKSLVDQPGKLPTVPKVVQQLMASFNAEDISAHEIAQQIATDPALSAKVLRLANSAFF 60 Query: 61 HASRTIGTVDDALRMLGFIMVRNLVLGNGMVAAFKSTRGMELPQFWRHTLYTAVAARWLS 120 HASRTIGTVDDALR+LGFIMVRNLVLGNGMVAAFK+TRGM+L QFWRHTLYTAVAARWL+ Sbjct: 61 HASRTIGTVDDALRILGFIMVRNLVLGNGMVAAFKNTRGMDLHQFWRHTLYTAVAARWLA 120 Query: 121 MHAGIHGEDNGDAVFTVGMMHGIGQLQLHAVAAATVAPLDKQCNVLDADRAEREKQTWGF 180 MHAG+ GEDNGDAVFTVGMMHGIGQLQLHAVAA VAPLD+ NVLD+DRAEREK+ WGF Sbjct: 121 MHAGMVGEDNGDAVFTVGMMHGIGQLQLHAVAANAVAPLDRHSNVLDSDRAEREKEAWGF 180 Query: 181 HYADVSAELAAIWNFPEALVTALRSVPDPLAAGRLDRAAAWVHLGAWAARVTVLSLDDEA 240 HYADVSAELAAIW+FP+ALVTALRSVPDPL AGR DRAAAWVHLG WAARV +LS DDE Sbjct: 181 HYADVSAELAAIWHFPDALVTALRSVPDPLGAGRFDRAAAWVHLGGWAARVAILSQDDEN 240 Query: 241 ALATYPVAVGKKLGVDPAWMPVLVKHDPDKAERAMPSLTELAAGLDAMLE 290 A ATYP AVGKKLGVDPAWMP+LVKHDPDKAERAMPSL ELAAGLDAMLE Sbjct: 241 AQATYPAAVGKKLGVDPAWMPLLVKHDPDKAERAMPSLGELAAGLDAMLE 290 >gi|300312232|ref|YP_003776324.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 274 Score = 240 bits (612), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 128/289 (44%), Positives = 177/289 (61%), Gaps = 16/289 (5%) Query: 1 MELKSLVDQPGKLPTIPKVVQQLMTSFNSEEVSAQDIAHQIAADPTLTAKLLRLANSAFF 60 M L++L Q LP+IPKVVQ+++ SFN+E VS ++IA ++AAD L+AK+LRLANS+++ Sbjct: 1 MNLEALFQQQTALPSIPKVVQEVIESFNNESVSIEEIAKKLAADQVLSAKILRLANSSYY 60 Query: 61 HASRTIGTVDDALRMLGFIMVRNLVLGNGMVAAFKSTRGMELPQFWRHTLYTAVAARWLS 120 H SRTIGTVDDA+ MLGF+ VR LV+ GM +FK G++L FWR++L TA+ A+WL+ Sbjct: 61 HVSRTIGTVDDAVLMLGFMTVRTLVVSTGMAGSFKKLPGVDLKLFWRYSLDTALVAKWLA 120 Query: 121 MHAGIHGEDNGDAVFTVGMMHGIGQLQLHAVAAATVAPLDKQCNVLDADRAEREKQTWGF 180 G+ N D FTVG+MH IGQL +HA +DK LDA R + E++++G+ Sbjct: 121 K----RGKLNSDFAFTVGLMHAIGQLVMHAGMPEQALQVDKVAGPLDARRLDVERKSFGY 176 Query: 181 HYADVSAELAAIWNFPEALVTALRSVPDPLAAGRLDRAAAWVHLGAWAARVTVLSLDDEA 240 +ADV AELA W FP+ + + P PL G D AA VHL AW AR DE Sbjct: 177 DFADVGAELARRWRFPDEFAKVIEAFPQPL-QGEFDPFAAIVHLAAWRARAHENQYSDEE 235 Query: 241 ALATYPVAVGKKLGVDPAWMPVLVKHDPDKAERAMPSLTELAAGLDAML 289 AT+P V ++LGV + + MP L EL G+ ++ Sbjct: 236 LAATFPAEVAERLGV-----------RLETFTQEMPPLEELGEGMQELI 273 >gi|300311595|ref|YP_003775687.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 310 Score = 66.6 bits (161), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 60/253 (23%), Positives = 108/253 (42%), Gaps = 16/253 (6%) Query: 13 LPTIPKVVQQLMTSFNSEEVSAQDIAHQIAADPTLTAKLLRLANSAFFHASRTIGTVD-D 71 LP V ++M E+++ ++ Q+ DP L +L+++AN + R + +V+ + Sbjct: 19 LPAPSGVRLKIMQMCQQEDIALPELVAQVQGDPVLAGRLIQIANIPNINKGRMVASVNVE 78 Query: 72 ALRMLGFIMVRNLVLGNGMVAAFKSTRGMELPQ--FWRHTLYTAVAARWLS--MHAGIHG 127 L M+G VR + L + + + E FW + A A + L + Sbjct: 79 LLLMVGLHAVRQMALAISLTESARRGDCAEFDYDAFWSRSTAMACAGQALGDLLQVAPPA 138 Query: 128 EDNGDAVFTVGMMHGIGQLQLHAVAAATVAPLDKQCNVLDADRAEREKQTWGFHYADVSA 187 E +FT+G++ GIGQL L + L DA +E++++GF + ++A Sbjct: 139 E-----MFTIGLLAGIGQLGLASARPKRYGEL-LLAGAKDAQLRVQERESFGFDHLALAA 192 Query: 188 ELAAIWNFPEALVTALRSVPDPLAAG-----RLDRAAAWVHLGAWAARVTVLSLDDEAAL 242 + W+FP A+ DP A+G R + + L A V S AAL Sbjct: 193 AMMQDWHFPRIFCEAVLHHRDPAASGLEEDDRQQKVTRLLELAGCVADVCTASDAQRAAL 252 Query: 243 ATYPVAVGKKLGV 255 + +G+ + Sbjct: 253 LPRLLQLGQHFAL 265 >gi|300310564|ref|YP_003774656.1| regulator of the two-component system protein [Herbaspirillum seropedicae SmR1] Length = 317 Score = 64.3 bits (155), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 50/186 (26%), Positives = 87/186 (46%), Gaps = 13/186 (6%) Query: 36 DIAHQIAADPTLTAKLLRLANSAFFHASRTIGTVD-DALRMLGFIMVRNLVLGNGM--VA 92 ++ H + ADP L+ +++R+ N R I +V D+L ++G VR L L + Sbjct: 42 ELIHLVQADPALSGRIIRIVNVLNAQRVRPIASVSMDSLLLVGLQAVRQLALALSLAETR 101 Query: 93 AFKSTRGMELPQFWRHTLYTAVAARWLSMHAGIH--GEDNGDAVFTVGMMHGIGQLQLHA 150 A S LP FW H+ A +A+ ++ + GE +FT G++ IG+L L + Sbjct: 102 APASCANFSLPAFWAHSFAMACSAQAVTSQLRVAPLGE-----MFTTGLLANIGKLTLAS 156 Query: 151 VAAATVAPLDKQCNVLDAD-RAEREKQTWGFHYADVSAELAAIWNFPEALVTALRSVPDP 209 + L + LDA R E+ +GF++ +++A L W P+ A+ P Sbjct: 157 AQPTGYSALLTR--ALDAPARRAMEQSLFGFNHLNIAAALMQDWKIPDLFCDAVLHYETP 214 Query: 210 LAAGRL 215 + G + Sbjct: 215 VVIGEI 220 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.132 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 891,772 Number of extensions: 35306 Number of successful extensions: 147 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 141 Number of HSP's successfully gapped: 4 Length of query: 290 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 206 Effective length of database: 1,222,130 Effective search space: 251758780 Effective search space used: 251758780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)