BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0029.0029.p
         (319 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311955|ref|YP_003776047.1| cobalamin biosynthesis CobD/Cbi...   447   e-127
gi|300310842|ref|YP_003774934.1| cobalamin biosynthesis transmem...    96   5e-21

>gi|300311955|ref|YP_003776047.1| cobalamin biosynthesis CobD/CbiB
           [Herbaspirillum seropedicae SmR1]
          Length = 317

 Score =  447 bits (1149), Expect = e-127,   Method: Compositional matrix adjust.
 Identities = 254/307 (82%), Positives = 271/307 (88%)

Query: 1   MLSGLSWPALAALMALGIALDLLLGEARRWHPLVGFGNLAKRIEGTLNHAPALRLRGVLA 60
           ML GLSWP LA LMALG+ALDL LGEARRWHPLVGFG LA+RIE  LN AP LRLRGVLA
Sbjct: 1   MLIGLSWPWLAVLMALGLALDLRLGEARRWHPLVGFGRLAQRIEAALNRAPGLRLRGVLA 60

Query: 61  WCLAVLPPVLACAWIIAVLPGWLAALAHAALLYFCIGLRSLREHTLPIAQALAQGDLPLA 120
           W LAVLPPVL  AW+IA  P WLAAL HA LLYFCIGLRSLREHTLPIAQAL  G+L  A
Sbjct: 61  WALAVLPPVLLAAWLIAQTPWWLAALLHAGLLYFCIGLRSLREHTLPIAQALGAGELAQA 120

Query: 121 RRLTSYIVSRDTRESGEAELAKAGVESLLENGNDAVFGTLFWFLVAGGPGALLFRLANTL 180
           RRLT++IVSRDTR + E ELAKAGVESLLENGNDAVFGTLFWFL+AGGPGALLFRL+NTL
Sbjct: 121 RRLTAWIVSRDTRSASEQELAKAGVESLLENGNDAVFGTLFWFLLAGGPGALLFRLSNTL 180

Query: 181 DAMWGYRNARFLFFGWSAARIDDGLNWLPARLTAFSYAWLGQRRQALDCWKAQAPAWSSP 240
           DAMWGYRNARFL+FGW+AARIDD LNW PARLTA SYA LGQRRQAL CWK QAPAWSSP
Sbjct: 181 DAMWGYRNARFLYFGWAAARIDDALNWCPARLTALSYAVLGQRRQALACWKEQAPAWSSP 240

Query: 241 NAGPVMAAGAGALGLALGGPAIYDGEVEERPPLGRGLPPQGPDIVRAWRLVAMSTALWVA 300
           NAGPVMAAGAGALGLALGG A+YDGEVE+RPPLG G+ PQG DIVRAWRLVAMS ALW+A
Sbjct: 241 NAGPVMAAGAGALGLALGGAAVYDGEVEQRPPLGCGVAPQGADIVRAWRLVAMSAALWMA 300

Query: 301 VVCALAV 307
           VV A+A+
Sbjct: 301 VVAAVAL 307


>gi|300310842|ref|YP_003774934.1| cobalamin biosynthesis
           transmembrane protein [Herbaspirillum seropedicae SmR1]
          Length = 319

 Score = 95.5 bits (236), Expect = 5e-21,   Method: Compositional matrix adjust.
 Identities = 78/259 (30%), Positives = 118/259 (45%), Gaps = 14/259 (5%)

Query: 38  NLAKRIEGTLNHAPALRLR-GVLAWCLAVLPPVLACAWIIAVLPGWLAALAHAALLYFCI 96
           + A  IEG  N       R G +A   A+  PV    W++  +    A   +  ++Y  +
Sbjct: 30  SFAATIEGWFNAGQVHHGRLGWVAMVGALTVPVALVYWLLLHVSLVAAFAWNVLVVYLTL 89

Query: 97  GLRSLREHTLPIAQALAQGDLPLARRLTSYIVSRDTRESGEAELAKAGVESLLENGNDAV 156
           G R    +   I  AL  GD   AR L +    +DT     +E+++   E  L   +  V
Sbjct: 90  GFRHYSHYFTSIQLALNSGDEMTARALLAEWTRQDTTGMDVSEISRIASERALITTHRHV 149

Query: 157 FGTLFWFLVAGGPG-ALLFRLANTLDAMWGY-RNARFLFFGWSAARIDDGLNWLPARLTA 214
           FG  FWFL+  GP  A+++R+A  L   W    + +F  FG  AA+    ++W+PARLTA
Sbjct: 150 FGVFFWFLMPVGPALAVMYRVAEYLSRAWNEPDHMKFEAFGRFAAQAFYWIDWIPARLTA 209

Query: 215 FSYAWLGQRRQALDCWKAQAPAWSSPNAGPVMAAGAGALGLALGG----------PAIYD 264
            ++A +G    A+  W+  A  W   +AG ++AAG GA+G+  G           PA   
Sbjct: 210 AAFAVVGNFEDAIYAWRNFASRWDDESAGIILAAGGGAMGVRFGTEKEAAAGIILPAD-A 268

Query: 265 GEVEERPPLGRGLPPQGPD 283
             V+  P    GLP   P 
Sbjct: 269 ATVDSMPEETDGLPGDTPS 287


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.325    0.139    0.457 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 899,370
Number of extensions: 35055
Number of successful extensions: 174
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 173
Number of HSP's successfully gapped: 2
Length of query: 319
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 234
Effective length of database: 1,217,395
Effective search space: 284870430
Effective search space used: 284870430
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 130 (54.7 bits)