BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0024.p (384 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311960|ref|YP_003776052.1| G3E family GTPase [Herbaspirill... 545 e-156 gi|300311859|ref|YP_003775951.1| CobW/P47K family protein [Herba... 84 2e-17 gi|300310263|ref|YP_003774355.1| cobalamin synthesis P47K protei... 82 7e-17 gi|300314043|ref|YP_003778135.1| cobalamin synthesis protein/P47... 75 6e-15 >gi|300311960|ref|YP_003776052.1| G3E family GTPase [Herbaspirillum seropedicae SmR1] Length = 377 Score = 545 bits (1405), Expect = e-156, Method: Compositional matrix adjust. Identities = 307/384 (79%), Positives = 323/384 (84%), Gaps = 12/384 (3%) Query: 3 SVVCTLP---PPLPPIPVTVVTGFLGAGKTTLLRGLVQRRQSRRLALLINEFGEVAIDGD 59 SV +LP P LPPIPVTVVTGFLGAGKTTLLRGL+QRRQSRRLALLINEFGEVAIDGD Sbjct: 2 SVALSLPELAPALPPIPVTVVTGFLGAGKTTLLRGLLQRRQSRRLALLINEFGEVAIDGD 61 Query: 60 LGPPVFXGDLLRAEGDAQDHVQIQDFAHGLIAYGDDEHFVPAMLALAARRAQIDHVLIET 119 L LRAEGDAQ VQIQDFAHGLIAYGDD HFVPAMLALAARRAQIDHVLIET Sbjct: 62 L---------LRAEGDAQGQVQIQDFAHGLIAYGDDAHFVPAMLALAARRAQIDHVLIET 112 Query: 120 SGLALPSAVMEVLQSPELAPHFVLDATLAVVDTPLLLEEAFASGDAVRDATAQLFRQQLE 179 SGLALPSAVMEVLQ E+A HFVLDATLAVVDTPLLLE+AFAS DAV ATAQLFRQQLE Sbjct: 113 SGLALPSAVMEVLQGAEMARHFVLDATLAVVDTPLLLEDAFASADAVGAATAQLFRQQLE 172 Query: 180 CADVVVLNKIDGMEEAPLLQAEETVRALAPNVRFLELAYGARLDIRLALGLRLHQAGSTV 239 CADVVVLNKIDGMEEAPLLQAE+ VRALAPNVRFLELAYGARLDIRLALGLRLHQAG+TV Sbjct: 173 CADVVVLNKIDGMEEAPLLQAEQAVRALAPNVRFLELAYGARLDIRLALGLRLHQAGATV 232 Query: 240 HHHYTPVGMPGERASVAADQWRFNGHAHSGLGAHSHGLSTHKHFHEQDPGWQSFTLRSQA 299 HHHYTPV MPGERA VA DQ RFNGH HSGLGAHSHGLSTHKHFHEQDPGWQSFTLRS A Sbjct: 233 HHHYTPVSMPGERAQVAPDQRRFNGHVHSGLGAHSHGLSTHKHFHEQDPGWQSFTLRSLA 292 Query: 300 AQAPESLRRAVVAVALDQPILRTKGFIATGEGSTQLLQAVRARAVVASAQGGKSAGGSEL 359 Q E LR+AV A AL +PILR KGF+ T +G+ + V S QGG G SEL Sbjct: 293 HQDAERLRQAVAAAALAEPILRAKGFVHTDQGARLVQAVRARVVVAESGQGGGRGGSSEL 352 Query: 360 VFIGYHPSRARVAQLLSSLTGTEW 383 VFIGYHPSRA+VA++LS TG +W Sbjct: 353 VFIGYHPSRAKVARILSETTGVDW 376 >gi|300311859|ref|YP_003775951.1| CobW/P47K family protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 84.0 bits (206), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 68/223 (30%), Positives = 111/223 (49%), Gaps = 29/223 (13%) Query: 15 IPVTVVTGFLGAGKTTLLRGLVQRRQSRRLALLINEFGEVAIDGDLGPPVFXG--DLLRA 72 +PVTV++GFLGAGKTTLL ++ R+ RR+A+++N+ EV ID L V G DL R Sbjct: 13 LPVTVLSGFLGAGKTTLLNHILHNREGRRVAVIVNDMSEVNIDAAL---VRDGGADLART 69 Query: 73 EGDAQDHVQIQDFAHGLIAYGDDEHFVPAMLALAARRAQIDHVLIETSGLALPSAVMEVL 132 E ++ + ++G I E + + L AR + D ++IE++G++ P V E Sbjct: 70 EE------KLVEMSNGCICCTLREDLLMEVRKL-ARERRFDQLVIESTGISEPLPVAETF 122 Query: 133 QSPE-----LAPHFVLDATLAVVDTPLLLEEAFASGDAV-----------RDATAQLFRQ 176 + L+ LD + VVD L++ ++S D++ L + Sbjct: 123 TFTDEDGASLSDVARLDTMVTVVDAFNFLKD-YSSQDSLDQRGQSMGEEDNRTVVDLLIE 181 Query: 177 QLECADVVVLNKIDGMEEAPLLQAEETVRALAPNVRFLELAYG 219 Q+E DV+VLNK+D + L + + L P R + +G Sbjct: 182 QIEFCDVIVLNKLDLVAADDLERLHAILHKLNPRARIVRAEFG 224 >gi|300310263|ref|YP_003774355.1| cobalamin synthesis P47K protein [Herbaspirillum seropedicae SmR1] Length = 413 Score = 82.0 bits (201), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 73/280 (26%), Positives = 136/280 (48%), Gaps = 38/280 (13%) Query: 15 IPVTVVTGFLGAGKTTLLRGLVQRRQSRRLALLINEFGEVAIDGDLGPPVFXGDLLRAEG 74 IPVTV++GFLG+GKTTLL +++ R ++A+++N+ EV +DGD D+ R Sbjct: 17 IPVTVLSGFLGSGKTTLLNHILRNRAGMKVAVIVNDMSEVNMDGD--------DVRRNTE 68 Query: 75 DAQDHVQIQDFAHGLIAYGDDEHFVPAMLALAARRAQIDHVLIETSGLALPSAVMEVL-- 132 + + ++ + ++G I + + AL AR + D++LIE++G++ P V E Sbjct: 69 LYRGNDELVEMSNGCICCTLRADLLEQVSAL-ARSGRFDYLLIESTGISEPIPVAETFAF 127 Query: 133 ---QSPELAPHFVLDATLAVVDTPLLLEE-------AFASGDAVRDATAQLFRQQLECAD 182 L+ LD + VV+ ++ A A G + R + L +Q+E A+ Sbjct: 128 LDSDGFSLSELARLDTLVTVVNGETFEQQLADHVAIAQADGASTR-PLSDLLIEQVEYAN 186 Query: 183 VVVLNKIDGMEEAPLLQAEETVRALAPNVRFLELAYGARLDIRLALGLRLHQAGSTVHHH 242 V++++++D ++ + + ++ L P R L +A+G +D+ L RL S Sbjct: 187 VILVSRVDLIDASAFASLKAVLQKLNPTARILPMAHG-EVDLDQVLDTRLFDLPSLAR-- 243 Query: 243 YTPVGMPGERASVAADQWRFNGHAHSGLGAHSHGLSTHKH 282 MPG ++ AH A ++G+S+ + Sbjct: 244 -----MPGWMQTMDG--------AHPPSEADTYGISSSVY 270 >gi|300314043|ref|YP_003778135.1| cobalamin synthesis protein/P47K family protein [Herbaspirillum seropedicae SmR1] Length = 388 Score = 75.5 bits (184), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 62/209 (29%), Positives = 101/209 (48%), Gaps = 22/209 (10%) Query: 14 PIPVTVVTGFLGAGKTTLLRGLVQRRQSRRLALLINEFGEVAIDGDLGPPVFXGDLLRAE 73 PIPV+++TGFLG+GKTTLL L+ + + + ++INEFGE+ +D LL A Sbjct: 11 PIPVSLLTGFLGSGKTTLLNHLITQPEMKDTLVIINEFGEIGLD----------HLLVAH 60 Query: 74 GDAQDHVQIQDFAHGLIAYGDDEHFVPAMLALAAR--RAQIDHVLIETSGLALPSAVMEV 131 + V++ GD + + + A R + + V+IET+GLA P+ ++ Sbjct: 61 SQEETIVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGRRKFNRVVIETTGLASPAPILHT 120 Query: 132 LQSPE-LAPHFVLDATLAVVDTPLLLEEAFASGDAVRDATAQLFRQQLECADVVVLNKID 190 L + +A + LD + VD +G + D + QQ AD ++L K D Sbjct: 121 LMTDAFIAERYRLDGVIVTVDA--------VNGMSTLDHHEEAV-QQAAVADRLLLTKTD 171 Query: 191 GMEEAPLLQAEETVRALAPNVRFLELAYG 219 ++ A L + +R L P R L+ G Sbjct: 172 LVDAATLAALQARLRTLNPGARQLQPQVG 200 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,049,987 Number of extensions: 41587 Number of successful extensions: 245 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 240 Number of HSP's successfully gapped: 4 Length of query: 384 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 297 Effective length of database: 1,207,925 Effective search space: 358753725 Effective search space used: 358753725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)