BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0378.p (225 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 394 e-111 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 155 2e-39 gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 132 3e-32 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 103 1e-23 gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspiri... 99 2e-22 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 394 bits (1011), Expect = e-111, Method: Compositional matrix adjust. Identities = 201/224 (89%), Positives = 214/224 (95%) Query: 1 MSYENILVETHDKVGLIRLNRPKALNALSDDLMSELGTALLAFDAQDEIGCIIVTGSEKA 60 MSYENI VETH+KVGLIRL+RPKALNALSD LM+ELG ALLAFDAQ+EIGCII+TGSEKA Sbjct: 1 MSYENIQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEKA 60 Query: 61 FAAGADISVMAGFDYMDVFNGEFITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDFI 120 FAAGADIS MAG+DYMDVF GEFITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDFI Sbjct: 61 FAAGADISAMAGYDYMDVFKGEFITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDFI 120 Query: 121 IAADNAKFGQPEIKLGIIPGAGGTQRLPRAVSKSKAMDLALTARMMDAQEAERAGLVSRI 180 IAADNAKFGQPEIKLGI+PGAGGTQRLPRAVSK+KAMDLALT RMMDA+EAERAGLVSRI Sbjct: 121 IAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLVSRI 180 Query: 181 VPAEKLMEEAMAAAVTITEMPRQVAMMVKDSVNRAYETTLSEXL 224 V A+KL+EEAMAAA+ I EMPRQVAMMVKDSVNRAYETTLSE + Sbjct: 181 VAADKLLEEAMAAAIIIAEMPRQVAMMVKDSVNRAYETTLSEGM 224 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 155 bits (393), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 89/228 (39%), Positives = 133/228 (58%), Gaps = 6/228 (2%) Query: 1 MSYENILVETH-DKVGLIRLNRPKALNALSDDLMSELGTALLAFDAQDEIGCIIVTGSEK 59 ++YE++L H + V + LNRP NAL + + E+ AL + D + ++++G+EK Sbjct: 5 VAYEDLLAARHGEHVLHLTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVISGNEK 64 Query: 60 AFAAGADISVMAGFDYMDVFNGEFITR--NWETLRRIRKPVIAAVAGYALGGGCELAMMC 117 AFAAGAD++ M + D + R W + R KP++AAV GYALG GCEL M Sbjct: 65 AFAAGADLNEMI---HKDAIATQLDVRAQYWRAIARFPKPILAAVNGYALGAGCELLMHA 121 Query: 118 DFIIAADNAKFGQPEIKLGIIPGAGGTQRLPRAVSKSKAMDLALTARMMDAQEAERAGLV 177 D IAA AK GQPEI +G +PGAGGTQRL R V K AM + L+ + A +A +AGLV Sbjct: 122 DIAIAARGAKIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGLV 181 Query: 178 SRIVPAEKLMEEAMAAAVTITEMPRQVAMMVKDSVNRAYETTLSEXLL 225 + +V + +E +A A +I + + K+++ +++E L LL Sbjct: 182 AEVVDDDATLERTLALAHSIAQKSPLAVRLAKEAMLQSFELGLEAGLL 229 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 132 bits (331), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 84/231 (36%), Positives = 127/231 (54%), Gaps = 9/231 (3%) Query: 1 MSYENILVETHDKVGLIRLNRPKALNALSDDLMSELGTALLAFDAQDEIGCIIVTGSEKA 60 MS +IL+E D + +I LNRP LN+ + + EL A+ A + +++TG+ + Sbjct: 1 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG 60 Query: 61 FAAGADI---SVMAGFDYMDVFNGEFITRNW----ETLRRIRKPVIAAVAGYALGGGCEL 113 F AG D+ +V G D +D+ GE I + + ++LR + PVI AV G A G G L Sbjct: 61 FCAGQDLGDRAVKPGDDGVDL--GESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANL 118 Query: 114 AMMCDFIIAADNAKFGQPEIKLGIIPGAGGTQRLPRAVSKSKAMDLALTARMMDAQEAER 173 + CD ++AA +A F + KLG+IP GGT LPR V ++AM +AL + A++AER Sbjct: 119 PLACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAER 178 Query: 174 AGLVSRIVPAEKLMEEAMAAAVTITEMPRQVAMMVKDSVNRAYETTLSEXL 224 GL+ + V ++LMEEA A A P + K ++ + TL E L Sbjct: 179 WGLIWKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQL 229 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 103 bits (258), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 69/195 (35%), Positives = 104/195 (53%), Gaps = 3/195 (1%) Query: 2 SYENILVETHDKVGLIRLNRPKALNALSDDLMSELGTALLAFDAQDEIGCIIVTGS-EKA 60 +Y N+ +E ++ L+ P A N + +++L + +A +I ++VTG EK Sbjct: 3 TYTNLKLEKTGHTAVLTLSNPPA-NTWTRAALADLTRLVKELNADRDIYTLVVTGEGEKF 61 Query: 61 FAAGADISVMAGFDY-MDVFNGEFITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDF 119 F+AGAD+ + A D M +ETL R IAA+ GYA+GGG E A+ CD Sbjct: 62 FSAGADLKLFADGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALACDI 121 Query: 120 IIAADNAKFGQPEIKLGIIPGAGGTQRLPRAVSKSKAMDLALTARMMDAQEAERAGLVSR 179 IA + A+ PE +G++P AGGTQ LP V + A + L ++A+ A R GLV Sbjct: 122 RIAEEQAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVEE 181 Query: 180 IVPAEKLMEEAMAAA 194 +VP + E A+A A Sbjct: 182 VVPRGQARERALALA 196 >gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 99.4 bits (246), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 68/230 (29%), Positives = 110/230 (47%), Gaps = 23/230 (10%) Query: 7 LVETHDKVGL--IRLNRPKALNALSDDLMSELGTALLAFDAQDEIGCIIVTGSEKAFAAG 64 ++E D+ G+ + LNRP+ NALS+++++ L L + + C+I+ + KAF AG Sbjct: 8 ILEREDQQGITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAANGKAFCAG 67 Query: 65 ADISVMAGFDYMDVFNGEF--ITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDFIIA 122 D+ M + F + ++LR + PVIA V G A GC+L CD IA Sbjct: 68 HDLRQMQATPQQTYYEALFHRCSHLMQSLRELPVPVIAKVQGLATAAGCQLVATCDLAIA 127 Query: 123 ADNAKFGQPEIKLGII---PGAGGTQRLPRAVSKSKAMDLALTARMMDAQEAERAGLVSR 179 A++A+F I +G+ P T+ LP +A ++ +T + +DA A GL+++ Sbjct: 128 AESARFAVSGINVGLFCSTPAVALTRNLP----IKRAFEMLVTGKFIDAATAAAWGLINQ 183 Query: 180 IVPAEKLMEEAMAAAVTITEMP------------RQVAMMVKDSVNRAYE 217 VP ++L A A I P RQ M + D+ A E Sbjct: 184 AVPEQELDSATQALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAE 233 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 637,989 Number of extensions: 25567 Number of successful extensions: 100 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 91 Number of HSP's successfully gapped: 10 Length of query: 225 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 143 Effective length of database: 1,231,600 Effective search space: 176118800 Effective search space used: 176118800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)