BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0371.p (226 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313180|ref|YP_003777272.1| phosphoglycolate phosphatase [H... 442 e-125 gi|300312992|ref|YP_003777084.1| phosphoglycolate phosphatase [H... 89 4e-19 gi|300313787|ref|YP_003777879.1| HAD-superfamily hydrolase [Herb... 64 2e-11 gi|300311212|ref|YP_003775304.1| phosphoglycolate phosphatase [H... 58 5e-10 >gi|300313180|ref|YP_003777272.1| phosphoglycolate phosphatase [Herbaspirillum seropedicae SmR1] Length = 226 Score = 442 bits (1136), Expect = e-125, Method: Compositional matrix adjust. Identities = 215/226 (95%), Positives = 220/226 (97%) Query: 1 MKNLTNIKAAIIDLDGTMLDTAADFHVAVNRMREELGLTPLSQETIVNFVGKGTENLIRR 60 M LTNIKAAIIDLDGTMLDTAADFHVAVNRMR++LGLTPLSQETIVNFVGKGTENLIRR Sbjct: 1 MSKLTNIKAAIIDLDGTMLDTAADFHVAVNRMRDDLGLTPLSQETIVNFVGKGTENLIRR 60 Query: 61 VLAVDYAEDEAAQYFQQALDSYTEHYLAINGDYSSLYPGVVEGLQALRAKGLRLACVTNK 120 VLAVDYAEDEAAQYFQQAL + TEHYLAINGDYSSLYPGV+EGLQALR KGLRLACVTNK Sbjct: 61 VLAVDYAEDEAAQYFQQALAACTEHYLAINGDYSSLYPGVLEGLQALRNKGLRLACVTNK 120 Query: 121 PLAFAVPLLQKKGLRDFFEIVYGGDSFPKKKPDPMPLLQVCEDFALQPAQVVAIGDSSND 180 PLAFAVPLLQKKGLRDFFEIVYGGDSFPKKKPDPMPLLQVC+DFAL PAQVVAIGDSSND Sbjct: 121 PLAFAVPLLQKKGLRDFFEIVYGGDSFPKKKPDPMPLLQVCQDFALAPAQVVAIGDSSND 180 Query: 181 AQAARAAGCRVLNVPYGYNHGESIHDVDSDGIVSTLVEAAQQISVD 226 AQAARAAGCRVLNVPYGYNHGESIHDVDSDGIVSTLVEAAQQISVD Sbjct: 181 AQAARAAGCRVLNVPYGYNHGESIHDVDSDGIVSTLVEAAQQISVD 226 >gi|300312992|ref|YP_003777084.1| phosphoglycolate phosphatase [Herbaspirillum seropedicae SmR1] Length = 218 Score = 88.6 bits (218), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 64/191 (33%), Positives = 87/191 (45%), Gaps = 5/191 (2%) Query: 8 KAAIIDLDGTMLDTAADFHVAVNRMREELGLTPLSQETIVNFVGKGTENLIRRVLAVDYA 67 +A + DLDGT+ DTA D A N +R++ GL E + G LI L + Sbjct: 7 RAILFDLDGTLADTAPDLAAAANFLRQQRGLEDAPYEQLRPVASAGARGLIGAALGIRPG 66 Query: 68 EDEAAQYFQQALDSYTEHYLAINGDYSSLYPGVVEGLQALRAKGLRLACVTNKPLAFAVP 127 EDE + + LD Y + +S L+ G+ E L L A+G+ VTNK F Sbjct: 67 EDEYEELRVRFLDRYQANMTT----HSRLFDGIPELLVQLEARGIAWGIVTNKAARFTDV 122 Query: 128 LLQKKGLRDFFEIVYGGDSFPKKKPDPMPLLQVCEDFALQPAQVVAIGDSSNDAQAARAA 187 L + GL IV GD+ P KP P PLL+ L P +GD D QA +AA Sbjct: 123 LAPQIGLGHAGCIV-SGDTTPHSKPHPAPLLEAARRLQLTPEDCWYVGDDLRDIQAGKAA 181 Query: 188 GCRVLNVPYGY 198 + +GY Sbjct: 182 AMPTIAAAWGY 192 >gi|300313787|ref|YP_003777879.1| HAD-superfamily hydrolase [Herbaspirillum seropedicae SmR1] Length = 278 Score = 63.5 bits (153), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 54/198 (27%), Positives = 82/198 (41%), Gaps = 17/198 (8%) Query: 3 NLTNIKAAIIDLDGTMLDTAADFHVAVNRMREELGLTPLSQETIVNFVGKGTENLIRRVL 62 +L I + DLDGT++D+ A H A N + + + +G + + Sbjct: 58 SLMPITHFLFDLDGTLMDSDALHHAAFNTILARWDRHIDVEYYKTHIMGASNAMIFGHLF 117 Query: 63 AVDYAE------DEAAQYFQQALDSYTEHYLAINGDYSSLYPGVVEGLQALRAKGLRLAC 116 AE +E F+ LD E PG+ L+ +R G R A Sbjct: 118 PGMPAEQYLPLAEEKESLFRSQLDQRVEPT-----------PGIERLLEHIRRIGGRSAV 166 Query: 117 VTNKPLAFAVPLLQKKGLRDFFEIVYGGDSFPKKKPDPMPLLQVCEDFALQPAQVVAIGD 176 VTN P A A +L+ GL F+ + GD + KPDP+P L + + VA D Sbjct: 167 VTNAPRANAELMLKATGLAGQFDTLVIGDELARAKPDPLPYLTGLQLLGGEAGAAVAFED 226 Query: 177 SSNDAQAARAAGCRVLNV 194 SS+ +AA +AG + Sbjct: 227 SSSGVKAASSAGIWTFGM 244 >gi|300311212|ref|YP_003775304.1| phosphoglycolate phosphatase [Herbaspirillum seropedicae SmR1] Length = 219 Score = 58.2 bits (139), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 49/189 (25%), Positives = 88/189 (46%), Gaps = 11/189 (5%) Query: 11 IIDLDGTMLDTAADFHVAVNRMREELGLTPLSQETIVNFV-GKGTENLIRRVLAVDYAED 69 + D DGT++D+ + + +LGL P+ ++ ++V G G ++ ++ + D Sbjct: 10 VFDWDGTLMDSTSTIVRCIQAAARDLGL-PIPDKSAASYVIGLGLQDAMQAAIP-----D 63 Query: 70 EAAQYFQQALDSYTEHYLAINGDYSSLYPGVVEGLQALRAKGLRLACVTNKPLAFAVPLL 129 +Y+ + ++ Y HYL + D + L+ GV E L L +G LA T K + Sbjct: 64 VDPKYYPRIVERYRHHYLGQDKDLT-LFEGVPEMLADLSQQGYFLAVATGKSRVGLNRAM 122 Query: 130 QKKGLRDFFEIVYGGD-SFPKKKPDPMPLLQVCEDFALQPAQVVAIGDSSNDAQAARAAG 188 GL F+ D +F KP P L ++ + + + IGD+++D Q A AG Sbjct: 123 NTTGLLSMFDASRCADETF--SKPHPAMLQELTRELGQDMHRTLMIGDTTHDLQMAINAG 180 Query: 189 CRVLNVPYG 197 + V +G Sbjct: 181 AAGVAVEFG 189 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 687,875 Number of extensions: 27634 Number of successful extensions: 105 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 103 Number of HSP's successfully gapped: 4 Length of query: 226 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 144 Effective length of database: 1,231,600 Effective search space: 177350400 Effective search space used: 177350400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)