BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0362.p (494 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 879 0.0 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 347 9e-97 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 332 3e-92 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 246 4e-66 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 121 1e-28 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 879 bits (2270), Expect = 0.0, Method: Compositional matrix adjust. Identities = 464/494 (93%), Positives = 473/494 (95%), Gaps = 1/494 (0%) Query: 1 MVQPETTEAKNTVGINDTSLPDARVRMLPYIVAATFFMEYLDTTIIATALPQMAQSFGVG 60 M PE+ +N V ND SL DARVRMLPYIVAATFFMEYLDTTIIATALPQMAQSFGVG Sbjct: 6 MEHPES-RGENNVATNDNSLTDARVRMLPYIVAATFFMEYLDTTIIATALPQMAQSFGVG 64 Query: 61 PNELSLGMTAYMLTLAVFIPISGWVADRFGSRTVFGIAIAIFTLASVLCGYSQNVWEFTA 120 PNELSLGMTAYMLTLAVFIPISGWVADRFGSRTVFG+AIAIFTLAS+LCG+SQNVWEFTA Sbjct: 65 PNELSLGMTAYMLTLAVFIPISGWVADRFGSRTVFGMAIAIFTLASILCGFSQNVWEFTA 124 Query: 121 ARVLQGLGGAMMVPVGRMIVVRSTGKDRLMKAIATITWPAIVAPVVGPSIGGFITTYASW 180 ARVLQGLGGAMMVPVGRMIVVRSTGKDRLMKAIATITWPAIVAPVVGPSIGGFITTYASW Sbjct: 125 ARVLQGLGGAMMVPVGRMIVVRSTGKDRLMKAIATITWPAIVAPVVGPSIGGFITTYASW 184 Query: 181 HWIFLMNVPFGFAAFIAVMRVVRNEHGHSKRPLDVIGFLLSGAALTSLLYGTEIASHMES 240 HWIFLMNVPFG AAF AVM VVRNEHG SKRPLD++GFLLSGAALTSLLYGTEIASHMES Sbjct: 185 HWIFLMNVPFGLAAFTAVMMVVRNEHGVSKRPLDLVGFLLSGAALTSLLYGTEIASHMES 244 Query: 241 NIKVAAGFVALGVVLGYCAWRHMQRIEHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPY 300 NIKVA GFVALGVVLGY AWRHMQRIEHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPY Sbjct: 245 NIKVALGFVALGVVLGYGAWRHMQRIEHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPY 304 Query: 301 LMPLMFQIGFGLTAFQSGLLLLATALGNLGMKAFTTRILQRFGFRSVAVNNAAIAGCLII 360 LMPLMFQIGFGLTAFQSGLLLLATALGNLGMKAFTTRILQRFGFRSVAVNNAAIAG LII Sbjct: 305 LMPLMFQIGFGLTAFQSGLLLLATALGNLGMKAFTTRILQRFGFRSVAVNNAAIAGGLII 364 Query: 361 ACGLLTPGTPLPVILGVIFCYGLSRSMQFSTLATLAYADISDARKSSASTLWSVAQQMTI 420 ACGLLTP TPLPVILGVIFCYGLSRSMQFSTLATLAYADISDARKSSASTLWSVAQQMTI Sbjct: 365 ACGLLTPATPLPVILGVIFCYGLSRSMQFSTLATLAYADISDARKSSASTLWSVAQQMTI 424 Query: 421 GMGIAFGALALRIAAAIRGEEIDATGAHFTLGDFQWAFAMAGVVTLISVIGYRGLARDAG 480 GMGIAFGALALRIAAA+RGE IDA+GAHFTLGDFQWAFAMAGVVTLISVIGYRGLARDAG Sbjct: 425 GMGIAFGALALRIAAALRGEAIDASGAHFTLGDFQWAFAMAGVVTLISVIGYRGLARDAG 484 Query: 481 SNIGGGMRAAQSKR 494 SNIGGGMRAA KR Sbjct: 485 SNIGGGMRAAPGKR 498 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 347 bits (891), Expect = 9e-97, Method: Compositional matrix adjust. Identities = 188/446 (42%), Positives = 272/446 (60%), Gaps = 8/446 (1%) Query: 41 LDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWVADRFGSRTVFGIAIA 100 +D+T+I+T+LP +A V P L L +T+Y+++LAVFIPISGW+ADRFGS T+F AI Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 101 IFTLASVLCGYSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTGKDRLMKAIATITWPA 160 +F L S+LCG S + +F AAR LQG+GGAMMVPVGR++++RS K L+KA++ +T PA Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 161 IVAPVVGPSIGGFITTYASWHWIFLMNVPFGFAAFIAVMRVVRNEHGHSKRPLDVIGFLL 220 + PV+GP +GGFITTY W WIF +N+P R + + PLD+ GFLL Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKGFLL 180 Query: 221 SGAALTSLLYGTEIASHMESNIKVAAGFVALGVV-LGYCAWRHMQRIEHPLLDFSNLRIP 279 S + L+ G A + +VA V G+V L W H +R PL+D + L+IP Sbjct: 181 SALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVW-HARRASAPLIDLALLKIP 239 Query: 280 TYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLLLLATALGNLGMKAFTTRIL 339 T V GS R+G+ A+P+L+PLM Q+GFG T F SG+L A+A G + MK R L Sbjct: 240 TLRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTIAARFL 299 Query: 340 QRFGFRSVAVNNAAIAGCLIIACGLLTPGTPLPVILGVIFCYGLSRSMQFSTLATLAYAD 399 +R+GFR V + N A+ + A TP TP +++ ++ G RS+QF++L ++YAD Sbjct: 300 RRYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNAISYAD 359 Query: 400 ISDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAAIRGEEIDATGAHFTLGDFQWAFA 459 + R S A+++ SVAQQ++IGMG+ AL+++ I+G A DF AF Sbjct: 360 VDKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGH------ATLVAQDFWPAFL 413 Query: 460 MAGVVTLISVIGYRGLARDAGSNIGG 485 + G V +++ R L DAG+ + G Sbjct: 414 VVGAVAACALLMLRQLPHDAGAEMAG 439 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 332 bits (852), Expect = 3e-92, Method: Compositional matrix adjust. Identities = 181/447 (40%), Positives = 272/447 (60%), Gaps = 8/447 (1%) Query: 41 LDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWVADRFGSRTVFGIAIA 100 +D T++AT+LP +A+ P L L MTAY+ L VFIPISGW+AD+ G+R VF A+ Sbjct: 1 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 60 Query: 101 IFTLASVLCGYSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTGKDRLMKAIATITWPA 160 +F S+LC S ++ F AAR LQG+GGAMMVPVGR+I+ RS K L+KA++ +T PA Sbjct: 61 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 120 Query: 161 IVAPVVGPSIGGFITTYASWHWIFLMNVPFGFAAFIAVMRVVRNEHGHSKRPLDVIGFLL 220 ++ PVVGP +GGFITTY W W+FL+N+PF F R ++N + PLDV GF+L Sbjct: 121 LLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKGFVL 180 Query: 221 SGAALTSLLYGTEI-ASHMESNIKVAAGFVALGVVLGYCAWRHMQRIEHPLLDFSNLRIP 279 + ++ G + SH+ A ++G + +RH +IE PLLDF LRIP Sbjct: 181 TSVGAAMIMLGLSLFGSHLVDGATPVA-MCSIGAIALVMYYRHSTQIEFPLLDFRLLRIP 239 Query: 280 TYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLLLLATALGNLGMKAFTTRIL 339 T +V+ GSL RIG+ AVP+L+PL Q G G+TAFQ+G + A+A G+L ++ R+L Sbjct: 240 TLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLAGRVL 299 Query: 340 QRFGFRSVAVNNAAIAGCLIIACGLLTPGTPLPVILGVIFCYGLSRSMQFSTLATLAYAD 399 +R+GFR+V + NA AG + A G+ + TP +I+ ++ G+ ++QF+ L ++AYAD Sbjct: 300 RRYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPALQFTCLNSIAYAD 359 Query: 400 ISDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAAIRGEEIDATGAHFTLGDFQWAFA 459 I ++L SV QQ+++G+G+ + L+++ ++G A DF AF Sbjct: 360 IDSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGH------ATIVEADFWPAFL 413 Query: 460 MAGVVTLISVIGYRGLARDAGSNIGGG 486 + G+ ++ SV L +AG + G Sbjct: 414 VIGLFSVASVPISGKLPHNAGLELSRG 440 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 246 bits (627), Expect = 4e-66, Method: Compositional matrix adjust. Identities = 157/452 (34%), Positives = 249/452 (55%), Gaps = 13/452 (2%) Query: 26 RMLPYIVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWV 85 R L +IVA+ FFM+ LDTTI+ TALP MA+S G + + AY LT+A P SGW+ Sbjct: 52 RALLWIVASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWL 111 Query: 86 ADRFGSRTVFGIAIAIFTLASVLCGYSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTG 145 ADRFG+R ++ AI +F+L S+LC + ++ AARV+QGLGG+M++PVGR+ V+R+ Sbjct: 112 ADRFGTRRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVP 171 Query: 146 KDRLMKAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGFAAFIAVMRVVRNE 205 + + A+A+I+ + PVVGP +GG++ SWHWIFL+N+P G AV+ + ++ Sbjct: 172 GAQYIAALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDD 231 Query: 206 HGHSKRPLDVIGFLLSGAALT--SLLYGTEIASHMESNIKVAAGFVALGVVLGYCAWRHM 263 D++G +L A + SL SH + +A VA VLGY WRH Sbjct: 232 SLAGTPRFDLLGCVLLSACMVAFSLALDVPFDSH-RTGWSIALFVVAALTVLGY--WRHA 288 Query: 264 QRIEHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLLLLA 323 PL P +S+ + + RIG AVP+L+PL+ Q+ G + +G++LL Sbjct: 289 SVHPRPLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLP 348 Query: 324 TALGNLGMKAFTTRILQRFGFRSVAVNNAAIAGCLIIACGLLTPGTPLPVILGVIFCYGL 383 A+ K + +++RFG+ + N AI G I++ L+TP PL + + +G Sbjct: 349 AAIAGTIAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIFGA 408 Query: 384 SRSMQFSTLATLAYADISDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAAIRGEEID 443 S SMQF+ + ++ + S ++L+S+ Q + IG+G+ G + + + G + Sbjct: 409 SNSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVNLFKSEVGTATN 468 Query: 444 ATGAHFTLGDFQWAFAMAGVVTLISVIGYRGL 475 A+ FQ FA+ G +TL S +R + Sbjct: 469 AS--------FQITFAIVGAITLASAWVFRKM 492 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 121 bits (303), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 113/425 (26%), Positives = 191/425 (44%), Gaps = 15/425 (3%) Query: 31 IVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWVADRFG 90 ++AA F+ LDTTI ++ +A + G ++ + +T+Y + A+ +P++GW+A+RFG Sbjct: 37 LLAAANFIAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFG 96 Query: 91 SRTVFGIAIAIFTLASVLCGYSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTGKDRLM 150 + VF +++ +F L S LCG + ++ A RVLQGL G ++P+ + +++R K++ Sbjct: 97 TVRVFILSMVMFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAP 156 Query: 151 KAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGF-AAFIAVMRVVRNEHGHS 209 A+ +VAP+ GP +GG + SW +IF +NVP +I + R E S Sbjct: 157 AAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGWRMLKRYETKMS 216 Query: 210 KRPLDVIGFLLS---GAALTSLLYGTEIASHMESNIKVAAGFVALGVVLGYCAWRHMQRI 266 K P+D +G +L AAL +L + S V A+ + + W +R Sbjct: 217 KLPIDRVGLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTER- 275 Query: 267 EHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLLLLATAL 326 +P++D R ++ +V T L L PL Q G TA SG + T + Sbjct: 276 -NPIVDLRVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCISALTGI 334 Query: 327 GNLGMKAFTTRILQRFGFRSVAVNNAAIAGCLIIACGLLTPGTPLPVILGVIFCYGLSRS 386 + ++ R + G + T I +F G+ Sbjct: 335 LAVMTAPMVAKMSTTIDARKLVCIGVTWLGFITFLRAFNTTDMSFFQIGWPLFLQGIGLP 394 Query: 387 MQFSTLATLAYADISDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAAIRGEEIDATG 446 + F L LA + +++ +SA+ L S + M GA+A I E DAT Sbjct: 395 LFFVPLTGLALSSVNEEETASAAGLMSFCRTMA-------GAIATSIVNT--SWENDATY 445 Query: 447 AHFTL 451 H L Sbjct: 446 FHAEL 450 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.138 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,314,370 Number of extensions: 48608 Number of successful extensions: 335 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 321 Number of HSP's successfully gapped: 17 Length of query: 494 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 405 Effective length of database: 1,198,455 Effective search space: 485374275 Effective search space used: 485374275 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)