BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0345.p (464 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysi... 836 0.0 gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmemb... 117 2e-27 gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirill... 100 3e-22 gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter prote... 72 1e-13 >gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 836 bits (2160), Expect = 0.0, Method: Compositional matrix adjust. Identities = 417/435 (95%), Positives = 427/435 (98%) Query: 4 MSIFTSLLLILLLVVVSAFLSCAEISIAASRKIRLEMMSKEGVHNADRVLSLQAQPGNFF 63 MSIFTSLLLILLLVVVSAFLSCAEISIAASRKIRLEMMSKEGVHNADRVLSLQAQPGNFF Sbjct: 1 MSIFTSLLLILLLVVVSAFLSCAEISIAASRKIRLEMMSKEGVHNADRVLSLQAQPGNFF 60 Query: 64 TVVQIGLNAVAILGGVVGEPALTPHMREIVSTFYVGPLLENISFAMSFLVVTAFFILFAD 123 TVVQIGLNAVAILGGVVGEPALTPHMREI+S+FY+GP LEN SFAMSFLVVTAFFILFAD Sbjct: 61 TVVQIGLNAVAILGGVVGEPALTPHMREIISSFYIGPWLENASFAMSFLVVTAFFILFAD 120 Query: 124 LMPKRLAMAAPERAAVALVQPMLLLETLFRPLVWFFNSLSKWIFTLFGLPQVRQDVITPS 183 LMPKRLAM APERAAVALV+PMLLLETLF+PLVWFFNSLSKWIFTLFGLPQVRQ+VITP+ Sbjct: 121 LMPKRLAMVAPERAAVALVKPMLLLETLFKPLVWFFNSLSKWIFTLFGLPQVRQEVITPN 180 Query: 184 EIHAMVDAGAQAGVLLTQEHHLIENVFELESRYVPSAMTPRDSIVYFLLTDSEDMIRQKI 243 EIHAMVDAGAQAGVLLTQEHHLIENVFELESRYVPSAMTPRDSIVYFLLTDSE IRQKI Sbjct: 181 EIHAMVDAGAQAGVLLTQEHHLIENVFELESRYVPSAMTPRDSIVYFLLTDSEATIRQKI 240 Query: 244 IDHPHSRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLADKGLVHTPLSIPDSLNLWEV 303 IDHPHSRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLADKGLVH PLSIPDSLNLWEV Sbjct: 241 IDHPHSRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLADKGLVHPPLSIPDSLNLWEV 300 Query: 304 LERMKAMDEDLAIVVNEYALVVGIISITDVTGVLMGNLLAITEEEQQIVRRDENSWLMDG 363 LERMKAMDEDLAIVVNEYALVVGIIS TDVTGVLMGNLLAITEEEQQIVRRDENSWLMDG Sbjct: 301 LERMKAMDEDLAIVVNEYALVVGIISTTDVTGVLMGNLLAITEEEQQIVRRDENSWLMDG 360 Query: 364 LTPLNDVMNELDIDEYPNPVGYETLSGFIMYMLRKIPKKTDFVLFSDFKFEVVDIEGNRI 423 LTPLNDVM+ELDIDEYPNPVGYETLSGFIMYMLRKIPKKTDFVLF++FKFEVVDIEGNRI Sbjct: 361 LTPLNDVMSELDIDEYPNPVGYETLSGFIMYMLRKIPKKTDFVLFAEFKFEVVDIEGNRI 420 Query: 424 NQLLVTRFKQDKGEK 438 NQLLVTRFKQDK EK Sbjct: 421 NQLLVTRFKQDKSEK 435 >gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 434 Score = 117 bits (293), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 104/438 (23%), Positives = 207/438 (47%), Gaps = 37/438 (8%) Query: 1 MNNMSIFTSLLLILLLVVVSAFLSCAEISIAASRKIRLEMMSKEGVHNADRVLSLQAQPG 60 M + I+ +L + +L+++SAF S E ++ A+ + RL ++K+G A +L L + Sbjct: 1 METVPIWVQVLALFILILLSAFFSMTETALMAANRHRLRHLAKQGNRAAITILWLLDRTE 60 Query: 61 NFFTVVQIG---LNAVAI-LGGVVGEPALTPHMREIVSTFYVGPLLENISFAMSFLVVTA 116 ++V I +NA+A L + A H R I + + V A Sbjct: 61 RLLSLVLIANTAINALATALVTALAIAAFGNHQRVITA---------------ATACVAA 105 Query: 117 FFILFADLMPKRLAMAAPERAAVA---LVQPMLLLETLFRPLVWFFNSLSKWIFTLFGL- 172 I+FA+++PK + PER ++ +++P++ + +PL+WF N I L + Sbjct: 106 LLIIFAEIVPKVIGATYPERISLFTGFILKPLM---RVAKPLIWFVNLFVSAILRLLRIK 162 Query: 173 --PQVRQDVITPSEIHAMVDAGAQAGVLLTQEHH-LIENVFELESRYVPSAMTPRDSIVY 229 + + I+P E+ ++V G G + Q+H ++ N+F+LE V MTPR + Sbjct: 163 TGARAAEQRISPEELRSIVLEG---GSFIPQKHKSILLNLFDLEKISVEDVMTPRSQVEA 219 Query: 230 FLLTDSEDMIRQKIIDHPHSRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLAD-KGLV 288 L D I ++ H++ V +G I+ ++G + + + + E+ S D + L+ Sbjct: 220 LNLAAPVDDILHQLATCYHNKLPVFEGEINHIVGILHVRKTVSLFRREEEISHDDIRELL 279 Query: 289 HTPLSIPDSLNLWEVLERMKAMDEDLAIVVNEYALVVGIISITDVTGVLMGNLLAIT--E 346 P +P +++ L+ + E L +VV+EY V G++++ D+ ++G + Sbjct: 280 SAPYYVPVETDVFTQLQYFQENHERLGVVVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGS 339 Query: 347 EEQQIVRRDENSWLMDGLTPLNDVMNELDIDEYPNPVGYETLSGFIMYMLRKIPKKTDFV 406 + + + L++G T L D+ L + + P G +T++G ++ L+ IP+ + V Sbjct: 340 SGEGFGWDKDGACLLEGSTTLRDINKRLGL-QLPLD-GPKTINGLLLEYLQDIPEASVAV 397 Query: 407 LFSDFKFEVVDIEGNRIN 424 E+V ++ I Sbjct: 398 KMDGCVIEIVQVQNQAIR 415 >gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 100 bits (248), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 96/426 (22%), Positives = 189/426 (44%), Gaps = 18/426 (4%) Query: 10 LLLILLLVVVSAFLSCAEISIAASRKIRLEMMSK-EGVHNADRVLSL-QAQPGNFFTVVQ 67 +++ LV ++ F AE S+ R+ R+ ++K +G+ R+L++ Q + + Q Sbjct: 7 IVVAFFLVALNGFFVAAEFSLVKLRQTRIRAIAKTQGMRG--RILAVVHNQLDAYLSACQ 64 Query: 68 IGLNAVAILGGVVGEPALTPHMREIVSTFYVG--PLLENISFAMSFLVVTAFFILFADLM 125 +G+ ++ G +GEPA + + S V L+ +SF +F V++ I+ +L Sbjct: 65 LGITLASLGLGWIGEPAFARILEPLFSLAGVTNQELIHGVSFVFAFFVISFLHIVAGELA 124 Query: 126 PKRLAMAAPERAAVALVQPMLLLETLFRPLVWFFNSLSKWIFTLFGL--PQVRQDVITPS 183 PK +A+ +PE+ + P+ PL+W N+ S W+ + GL + Sbjct: 125 PKSMAIRSPEKLGLWCAMPLYGFYWGMYPLIWVLNASSNWLLRVAGLGAGHGHDAHYSSD 184 Query: 184 EIHAMVDAGAQA---GVLLTQEHHLIENVFELESRYVPSAMTPRDSIVYFLLTDSEDMIR 240 E+ ++ AG ++ G E +++ V M P IV S + Sbjct: 185 ELKLILRAGNKSGKNGKFTRDEWNVLTQSLNFAELDVADIMRPASEIVALGDDKSLEENL 244 Query: 241 QKIIDHPHSRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLAD-KGLVHTPLSIPDSLN 299 I + +SR+ D VLG + KDV L + + ++A+ K + I +L Sbjct: 245 DIIYRNRYSRYPYYDAERQQVLGLVHLKDVF--LAQQDGRAIANLKDYLRPVQYISPALP 302 Query: 300 LWEVLERMKAMDEDLAIVVNEYALVVGIISITDVTGVLMGNLL-AITEEEQQIVRRDENS 358 ++L R + A++ + G I++ ++ +L+G + + R+D+ + Sbjct: 303 ALDLLRRFRTGSPHFAVIGKKGQPPAGFITLDNMLSLLVGEIRDEFRHNTGEWTRQDDGT 362 Query: 359 WLMDGLTPLNDVMNELDID-EYPNPVGYETLSGFIMYMLRKIPKKTDFVLFSDFKFEVVD 417 L G P+ + N L ID EY + + +++ G +M L +PK+ + F F + Sbjct: 363 LLGKGSLPIVTLENMLGIDIEYDDSI--DSVGGLVMEKLGDLPKEGQKIDFPAFDVVIKR 420 Query: 418 IEGNRI 423 + G +I Sbjct: 421 MSGPKI 426 >gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter protein [Herbaspirillum seropedicae SmR1] Length = 292 Score = 72.0 bits (175), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 62/245 (25%), Positives = 114/245 (46%), Gaps = 34/245 (13%) Query: 205 LIENVFELESRYVPSAMTPRDSIVYFLLTDSEDMIRQKIIDHPHSRFLVCDGHIDAVLGY 264 +IE VF++ V M PR + ++ + +++ HSRF DG D V+G Sbjct: 56 MIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSHSRFPAVDGERDKVVGI 115 Query: 265 IDSKDVLKRLLKGEQFSLADKGLVHTPLSIPDSLNLWEVLERMKAMDEDLAIVVNEYALV 324 + +KD+L R E F + D ++ + IP+S L +L +A +A+VV+EY V Sbjct: 116 LLAKDLL-RYYAEESFDVRD--MLRPAIFIPESKRLNVLLRDFRANRNHMAVVVDEYGGV 172 Query: 325 VGIISITDVTGVLMGNLLAITEEEQQIVRRDEN-----------SWLMDGLTPLNDVMNE 373 G+I+I DV ++G++ E+E ++N W + LT Sbjct: 173 AGLITIEDVLEQIVGDI----EDEYDFDEAEDNVLALKDGPHGARWRVKALT-------- 220 Query: 374 LDIDEYPNPVG-------YETLSGFIMYMLRKIPKKTDFVLFSDFKFEVVDIEGNRINQL 426 +I+++ VG +T+ G + + ++P+K D FEV+ + +++ L Sbjct: 221 -EIEQFNEEVGSRFSDDDADTIGGLVANHVGRVPRKGDEFDIDGLHFEVLRADARQVHVL 279 Query: 427 LVTRF 431 LV++ Sbjct: 280 LVSQL 284 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,236,666 Number of extensions: 51750 Number of successful extensions: 152 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 146 Number of HSP's successfully gapped: 4 Length of query: 464 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 375 Effective length of database: 1,198,455 Effective search space: 449420625 Effective search space used: 449420625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)