BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0342.p (460 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 855 0.0 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 509 e-145 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 317 9e-88 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 239 3e-64 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 115 7e-27 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 855 bits (2209), Expect = 0.0, Method: Compositional matrix adjust. Identities = 431/441 (97%), Positives = 437/441 (99%) Query: 20 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSWAMI 79 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFS AMI Sbjct: 1 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 60 Query: 80 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 139 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA Sbjct: 61 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 120 Query: 140 LLGPVVGPLLGGFLTTYFHWRWVFLINIPFCFLGLYLAKRFIQNFKEDNPAPLDVKGFVL 199 LLGPVVGPLLGGF+TTYFHWRWVFLINIPFCFLGLYLA+RFIQNFKEDNPAPLDVKGFVL Sbjct: 121 LLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKGFVL 180 Query: 200 TSVGAAMIMLGLSLFGSQLVDGATPVAMCSIGAIALVMYYRHSTQIPYPLLDFRLLRIPT 259 TSVGAAMIMLGLSLFGS LVDGATPVAMCSIGAIALVMYYRHSTQI +PLLDFRLLRIPT Sbjct: 181 TSVGAAMIMLGLSLFGSHLVDGATPVAMCSIGAIALVMYYRHSTQIEFPLLDFRLLRIPT 240 Query: 260 LHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLASRVLR 319 LHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLA RVLR Sbjct: 241 LHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLAGRVLR 300 Query: 320 RYGFRTVLMVNAVFAGIALAAYGIFSMHTPYLLIVTIVLMGGVFPALQFTCLNSIAYADI 379 RYGFRTVLMVNAVFAGIALAAYGIFS+HTPYLLIV+IVLMGGVFPALQFTCLNSIAYADI Sbjct: 301 RYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPALQFTCLNSIAYADI 360 Query: 380 DSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHASIVEADFWPAFLVIGLFSV 439 DSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHA+IVEADFWPAFLVIGLFSV Sbjct: 361 DSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHATIVEADFWPAFLVIGLFSV 420 Query: 440 ASVPISGKLPHNAGLELSRGK 460 ASVPISGKLPHNAGLELSRGK Sbjct: 421 ASVPISGKLPHNAGLELSRGK 441 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 509 bits (1310), Expect = e-145, Method: Compositional matrix adjust. Identities = 249/438 (56%), Positives = 330/438 (75%) Query: 20 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSWAMI 79 MD+TV++TSLPALA D++ DPI+LKLA+T+Y+ +L VFIPISGWMAD+ G+ +F A+ Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 80 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 139 VFM GSILC VS +L FVAARFLQGIGGAMMVPVGR++I RSV+K LVKA+SYLT+PA Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 140 LLGPVVGPLLGGFLTTYFHWRWVFLINIPFCFLGLYLAKRFIQNFKEDNPAPLDVKGFVL 199 L GPV+GP LGGF+TTYFHWRW+F INIP LGLYLA RFI + K + APLD+KGF+L Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKGFLL 180 Query: 200 TSVGAAMIMLGLSLFGSQLVDGATPVAMCSIGAIALVMYYRHSTQIPYPLLDFRLLRIPT 259 +++G +++M+GL+ G LV G + G + L Y H+ + PL+D LL+IPT Sbjct: 181 SALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLKIPT 240 Query: 260 LHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLASRVLR 319 L A V GGS +R+G+GA+PFLLPL LQ G G T F +G +TCASA G++F++ +A+R LR Sbjct: 241 LRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTIAARFLR 300 Query: 320 RYGFRTVLMVNAVFAGIALAAYGIFSMHTPYLLIVTIVLMGGVFPALQFTCLNSIAYADI 379 RYGFR VL+VN + L A F+ TPYLL+V I+L+GG F +LQFT LN+I+YAD+ Sbjct: 301 RYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNAISYADV 360 Query: 380 DSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHASIVEADFWPAFLVIGLFSV 439 D + TS+ASV QQLS+G+GV +AG LQ+SN +QGHA++V DFWPAFLV+G + Sbjct: 361 DKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVAQDFWPAFLVVGAVAA 420 Query: 440 ASVPISGKLPHNAGLELS 457 ++ + +LPH+AG E++ Sbjct: 421 CALLMLRQLPHDAGAEMA 438 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 317 bits (813), Expect = 9e-88, Method: Compositional matrix adjust. Identities = 190/465 (40%), Positives = 286/465 (61%), Gaps = 16/465 (3%) Query: 6 LIPLIVGCALFMENMDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMA 65 ++P IV FME +D T++AT+LP +A+ P L L MTAY+ L VFIPISGW+A Sbjct: 31 MLPYIVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWVA 90 Query: 66 DKLGARKVFSWAMIVFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDK 125 D+ G+R VF A+ +F SILC S ++ F AAR LQG+GGAMMVPVGR+I+ RS K Sbjct: 91 DRFGSRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTGK 150 Query: 126 SELVKAVSYLTLPALLGPVVGPLLGGFLTTYFHWRWVFLINIPFCFLGLYLAKRFIQNFK 185 L+KA++ +T PA++ PVVGP +GGF+TTY W W+FL+N+PF ++N Sbjct: 151 DRLMKAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGLAAFTAVMMVVRNEH 210 Query: 186 EDNPAPLDVKGFVLTSVGAAMIMLGLSLFGSQLVDGATPVAMCSIGAIAL--VMYY---R 240 + PLD+ GF+L+ GAA+ L L+G+++ ++G +AL V+ Y R Sbjct: 211 GVSKRPLDLVGFLLS--GAALTSL---LYGTEIASHMESNIKVALGFVALGVVLGYGAWR 265 Query: 241 HSTQIPYPLLDFRLLRIPTLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSIT 300 H +I +PLLDF LRIPT +V+ GSL RIG+ AVP+L+PL Q G G+TAFQ+G + Sbjct: 266 HMQRIEHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLLL 325 Query: 301 CASAFGSLFIRLLASRVLRRYGFRTVLMVNAVFAGIALAAYGIFSMHTPYLLIVTIVLMG 360 A+A G+L ++ +R+L+R+GFR+V + NA AG + A G+ + TP +I+ ++ Sbjct: 326 LATALGNLGMKAFTTRILQRFGFRSVAVNNAAIAGGLIIACGLLTPATPLPVILGVIFCY 385 Query: 361 GVFPALQFTCLNSIAYADIDSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGH- 419 G+ ++QF+ L ++AYADI ++L SV QQ+++G+G+ + L+++ L+G Sbjct: 386 GLSRSMQFSTLATLAYADISDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAALRGEA 445 Query: 420 -----ASIVEADFWPAFLVIGLFSVASVPISGKLPHNAGLELSRG 459 A DF AF + G+ ++ SV L +AG + G Sbjct: 446 IDASGAHFTLGDFQWAFAMAGVVTLISVIGYRGLARDAGSNIGGG 490 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 239 bits (610), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 147/442 (33%), Positives = 246/442 (55%), Gaps = 9/442 (2%) Query: 10 IVGCALFMENMDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLG 69 IV FM+ +D T++ T+LPA+AR L + + + + AY + P SGW+AD+ G Sbjct: 57 IVASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFG 116 Query: 70 ARKVFSWAMIVFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELV 129 R+++ A+++F GS+LCA++ SL +AAR +QG+GG+M++PVGR+ + R+V ++ + Sbjct: 117 TRRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYI 176 Query: 130 KAVSYLTLPALLGPVVGPLLGGFLTTYFHWRWVFLINIPFCFLGLYLAKRFIQNFKEDNP 189 A++ +++ LGPVVGP+LGG+L W W+FLIN+P LG+ F+ + Sbjct: 177 AALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLAGT 236 Query: 190 APLDVKGFVLTSVGAAMIMLGLSL---FGSQLVDGATPVAMCSIGAIALVMYYRHSTQIP 246 D+ G VL S A M+ L+L F S + +A+ + A+ ++ Y+RH++ P Sbjct: 237 PRFDLLGCVLLS--ACMVAFSLALDVPFDSHRTGWS--IALFVVAALTVLGYWRHASVHP 292 Query: 247 YPLLDFRLLRIPTLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFG 306 PL L P + G + RIG AVPFLLPL LQ LG + AG + +A Sbjct: 293 RPLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPAAIA 352 Query: 307 SLFIRLLASRVLRRYGFRTVLMVNAVFAGIALAAYGIFSMHTPYLLIVTIVLMGGVFPAL 366 + + +++R+G+ L+VN G A+ ++ + + P + + + G ++ Sbjct: 353 GTIAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIFGASNSM 412 Query: 367 QFTCLNSIAYADIDSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHASIVEAD 426 QF +NS+ +D RDAG SL S++Q L++GLGVT+ G ++ L G A+ A Sbjct: 413 QFAAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVNLFKSEVGTAT--NAS 470 Query: 427 FWPAFLVIGLFSVASVPISGKL 448 F F ++G ++AS + K+ Sbjct: 471 FQITFAIVGAITLASAWVFRKM 492 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 115 bits (288), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 82/295 (27%), Positives = 147/295 (49%), Gaps = 4/295 (1%) Query: 9 LIVGCALFMENMDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKL 68 +++ A F+ +D T+ S+ +A L +T+Y A + +P++GW+A++ Sbjct: 36 ILLAAANFIAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRF 95 Query: 69 GARKVFSWAMIVFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSEL 128 G +VF +M++F S LC ++ SL +A R LQG+ G ++P+ + ++ R K + Sbjct: 96 GTVRVFILSMVMFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKA 155 Query: 129 VKAVSYLTLPALLGPVVGPLLGGFLTTYFHWRWVFLINIPFCFLGLYLAKRFIQNFKED- 187 AV + L+ P+ GP+LGG L + W ++F IN+P + Y+ R ++ ++ Sbjct: 156 PAAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGWRMLKRYETKM 215 Query: 188 NPAPLDVKGFVLTSVGAAMIMLGLSLFGSQLVDGATPVAMCSIGAIA-LVMYYRHSTQIP 246 + P+D G VL V A + L L + +T + + ++ A+ + + Sbjct: 216 SKLPIDRVGLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTER 275 Query: 247 YPLLDFRLLRIPTLHASVVGGSL-FRIGLGAVPFLLPLALQEGLGMTAFQAGSIT 300 P++D R+ R ASV L F G+V L PL LQ +G TA +G I+ Sbjct: 276 NPIVDLRVFRHRGFTASVTTICLAFGAFFGSV-VLTPLWLQGYMGYTATWSGCIS 329 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.143 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,353,886 Number of extensions: 56621 Number of successful extensions: 353 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 337 Number of HSP's successfully gapped: 18 Length of query: 460 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 372 Effective length of database: 1,203,190 Effective search space: 447586680 Effective search space used: 447586680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)